Single-cell gel electrophoresis (SCG)-A review and discussion.

PubWeight™: 0.85‹?›

🔗 View Article (PMC 2723433)

Published in Environ Health Prev Med on July 01, 1997

Authors

Y Lu1, T Takeshita, K Morimoto

Author Affiliations

1: Department of Hygiene and Preventive Medicine, Osaka University School of Medicine, 2-2 Yamadaoka, Suita, 565, Osaka, Japan.

Articles cited by this

A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res (1988) 17.88

The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutat Res (1995) 1.61

Antioxidant supplementation decreases oxidative DNA damage in human lymphocytes. Cancer Res (1996) 1.46

bcl-2 protein inhibits etoposide-induced apoptosis through its effects on events subsequent to topoisomerase II-induced DNA strand breaks and their repair. Cancer Res (1993) 1.43

The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutat Res (1994) 1.41

Gel electrophoresis of individual cells to quantify hypoxic fraction in human breast cancers. Cancer Res (1993) 1.25

Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int J Radiat Biol (1992) 1.16

Radiation-induced apoptosis measured in TK6 human B lymphoblast cells using the comet assay. Radiat Res (1993) 1.13

DNA fragmentation during apoptosis is caused by frequent single-strand cuts. Nucleic Acids Res (1993) 1.07

Detection of hypoxic cells in a murine tumor with the use of the comet assay. J Natl Cancer Inst (1992) 1.05

A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides. Mutat Res (1991) 1.03

Detection of DNA-crosslinking agents with the alkaline comet assay. Environ Mol Mutagen (1996) 1.00

The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment. Mutagenesis (1995) 0.98

Detection of apoptosis induced DNA cleavage in scrapie-infected sheep brain. FEMS Microbiol Lett (1994) 0.97

Comparative investigations of the genotoxic effects of metals in the single cells gel (SCG) assay and the sister chromatid exchange (SCE) test. Environ Mol Mutagen (1994) 0.95

Hypoxic fractions measured in murine tumors and normal tissues using the comet assay. Int J Radiat Oncol Biol Phys (1994) 0.95

Genotoxic activity of potassium permanganate in acidic solutions. Mutat Res (1991) 0.94

The single cell gel electrophoresis/comet assay: a potential tool for detecting radiation-induced DNA damage in humans. Stem Cells (1995) 0.94

Comet assay reveals DNA strand breaks induced by ultrasonic cavitation in vitro. Ultrasound Med Biol (1995) 0.94

Endogenous nitric oxide induced by interleukin-1 beta in rat islets of Langerhans and HIT-T15 cells causes significant DNA damage as measured by the 'comet' assay. FEBS Lett (1993) 0.92

Does physical activity induce DNA damage? Mutagenesis (1994) 0.90

Wavelength dependence of laser-induced DNA damage in lymphocytes observed by single-cell gel electrophoresis. J Photochem Photobiol B (1995) 0.90

Comparative studies by comet test and SCE analysis in human lymphocytes from 200 healthy subjects. Mutat Res (1995) 0.90

Herbicide-induced DNA damage in human lymphocytes evaluated by the single-cell gel electrophoresis (SCGE) assay. Mutat Res (1995) 0.89

Detection of hypoxic fractions in murine tumors by comet assay: comparison with other techniques. Radiat Res (1995) 0.89

Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutat Res (1994) 0.89

Assessment of genotoxic effects by lindane. Food Chem Toxicol (1993) 0.88

Radiation-induced DNA double-strand breaks produced in histone-depleted tumor cell nuclei measured using the neutral comet assay. Radiat Res (1995) 0.87

Effect of diet and vitamin C on DNA strand breakage in freshly-isolated human white blood cells. Mutat Res (1994) 0.86

The alkaline comet test on plant cells: a new genotoxicity test for DNA strand breaks in Vicia faba root cells. Mutat Res (1996) 0.86

Assessment of radiation-induced DNA damage in human blood lymphocytes using the single-cell gel electrophoresis technique. Mutat Res (1992) 0.86

An analysis of gamma-ray-induced DNA damage in human blood leukocytes, lymphocytes and granulocytes. Mutat Res (1993) 0.84

Vitamin E prevents exercise-induced DNA damage. Mutat Res (1995) 0.84

Response of fibroblast cultures from ataxia-telangiectasia patients to reactive oxygen species generated during inflammatory reactions. Environ Mol Mutagen (1994) 0.84

DNA damage following combination of radiation with the bioreductive drug AQ4N: possible selective toxicity to oxic and hypoxic tumour cells. Br J Cancer (1996) 0.83

Alkaline single cell gel (comet) assay and genotoxicity monitoring using bullheads and carp. Environ Mol Mutagen (1995) 0.83

Comet assay studies of radiation-induced DNA damage and repair in various tumour cell lines. Int J Radiat Biol (1994) 0.82

Biomarkers of styrene exposure in lamination workers: levels of O6-guanine DNA adducts, DNA strand breaks and mutant frequencies in the hypoxanthine guanine phosphoribosyltransferase gene in T-lymphocytes. Carcinogenesis (1995) 0.82

In vitro toxicity of 1,2-dibromo-3-chloropropane (DBCP) in different testicular cell types from rats. Reprod Toxicol (1996) 0.81

Differences in basal and induced DNA single-strand breaks between human peripheral monocytes and lymphocytes. Mutat Res (1995) 0.81

High-dose combination alkylating agents with autologous bone-marrow support in patients with breast cancer: preliminary assessment of DNA damage in individual peripheral blood lymphocytes using the single cell gel electrophoresis assay. Mutat Res (1992) 0.81

Analysis of single-cell gel electrophoresis using laser-scanning microscopy. Mutat Res (1993) 0.80

Hypersensitivity of human lymphocytes to UV-B and solar irradiation. Cancer Res (1993) 0.80

Reproducibility of basal and induced DNA single-strand breaks detected by the single-cell gel electrophoresis assay in human peripheral mononuclear leukocytes. Int Arch Occup Environ Health (1995) 0.79

Early effects of benzene exposure in mice. Hematological versus genotoxic effects. Arch Toxicol (1994) 0.79

Comet assay to measure DNA damage in apoptotic cells. J Biochem Biophys Methods (1995) 0.79

Detection of DNA damaging agents in environmental water samples. Bull Environ Contam Toxicol (1994) 0.79

Single cell gel electrophoresis assay in the earthworm for the detection of genotoxic compounds in soils. Bull Environ Contam Toxicol (1995) 0.79

Styrene oxide-induced HPRT mutations, DNA adducts and DNA strand breaks in cultured human lymphocytes. Carcinogenesis (1995) 0.79

Benzene-induced genotoxicity in mice in vivo detected by the alkaline comet assay: reduction by CYP2E1 inhibition. Mutat Res (1996) 0.78

Measuring the repair of H2O2-induced DNA single strand breaks using the single cell gel assay. Cytobios (1993) 0.78

A sensitivity to oxidative stress is linked to chromosome 11 but is not due to a difference in single strand DNA breakage or repair. Mutat Res (1993) 0.78

Induction of genotoxicity by salted deep-fried fish and mutton. Mutat Res (1994) 0.78

Cytotoxic and clastogenic effects of benzyl isothiocyanate towards cultured mammalian cells. Food Chem Toxicol (1995) 0.78

Rat mesothelial and tracheal epithelial cells show equal DNA sensitivity to hydrogen peroxide-induced oxidant injury. Am J Physiol (1995) 0.78

Assessment of smoking-induced DNA damage in lymphocytes of smoking mothers of newborn infants using the alkaline single-cell gel electrophoresis technique. Mutat Res (1995) 0.78

An investigation of the DNA-damaging ability of benzene and its metabolites in human lymphocytes, using the comet assay. Environ Mol Mutagen (1995) 0.78

Genotoxicity of vanadium pentoxide evaluate by the single cell gel electrophoresis assay in human lymphocytes. Mutat Res (1996) 0.78

Assessment of DNA damage in canine peripheral blood and bone marrow after total body irradiation using the single-cell gel electrophoresis technique. Mutat Res (1996) 0.77

Cytogenetic studies of rodents exposed to styrene by inhalation. IARC Sci Publ (1993) 0.77

Single-cell gel technique supports hit probability calculations. Health Phys (1993) 0.77

The role of oxygenation in embryotoxic mechanisms of three bioreducible agents. Teratology (1993) 0.76

Reduction of benzene toxicity by toluene. Environ Mol Mutagen (1994) 0.76

A single cell gel electrophoresis technique for the detection of DNA damage induced by ACNU, an alkylating agent or irradiation in murine glioma cell lines. Neurol Res (1994) 0.76

UV-B-laser-induced DNA damage in lymphocytes observed by single-cell gel electrophoresis. J Photochem Photobiol B (1994) 0.76

Ultraviolet-light exposure induces a heritable sensitivity to the induction of SCE by mitomycin-C. Mutat Res (1985) 0.76

Laser scanning microscopic analysis of DNA damage in frozen tissues. Cancer Lett (1994) 0.76

Determination of radiation-induced damage in lymphocytes using the micronucleus and microgel electrophoresis 'Comet' assays. Eur J Cancer (1995) 0.76

Application of confocal laser scanning microscopy to analysis of H2O2-induced DNA damage in human cells. Scanning (1994) 0.76

DNA-damage detection in man after radiation exposure--the comet assay--its possible application for human biomonitoring. Stem Cells (1995) 0.76

Use of the comet assay to detect hypoxic cells in murine tumours and normal tissues exposed to bioreductive drugs. Acta Oncol (1995) 0.76

Demonstration of chlorobenzene-induced DNA damage in mouse lymphocytes using the single cell gel electrophoresis assay. Toxicology (1995) 0.76

Cytogenetic analyses of the in vitro and in vivo responses of murine cells to peroxyacetyl nitrate (PAN). Mutat Res (1995) 0.76

Comet assay analysis of repair of DNA strand breaks in normal and deficient human cells exposed to radiations and chemicals. Evidence for a repair pathway specificity of DNA ligation. Radiat Res (1995) 0.76

Genotoxicity of m-phenylenediamine and 2-aminofluorene in Salmonella typhimurium and human lymphocytes with and without plant activation. Environ Mol Mutagen (1995) 0.76

Aflatoxin B1, 2-aminoanthracene, and 7,12-dimethylbenz[a]anthracene-induced frameshift mutations in human APRT. Environ Mol Mutagen (1995) 0.75

Articles by these authors

Sharing of the interleukin-2 (IL-2) receptor gamma chain between receptors for IL-2 and IL-4. Science (1993) 4.39

Cyclosporine induces cancer progression by a cell-autonomous mechanism. Nature (1999) 3.93

Tissue pressure measurements as a determinant for the need of fasciotomy. Clin Orthop Relat Res (1976) 3.32

Calcium current-dependent and voltage-dependent inactivation of calcium channels in Helix aspersa. J Physiol (1981) 3.23

Induction of CD8+ cytotoxic T cells by immunization with purified HIV-1 envelope protein in ISCOMs. Nature (1990) 3.15

Functional participation of the IL-2 receptor gamma chain in IL-7 receptor complexes. Science (1994) 2.78

The interleukin-2 receptor gamma chain: its role in the multiple cytokine receptor complexes and T cell development in XSCID. Annu Rev Immunol (1996) 2.50

Validation study of the in vitro micronucleus test in a Chinese hamster lung cell line (CHL/IU). Mutagenesis (1999) 2.16

Collaboration of antibody and inflammation in clearance of rabies virus from the central nervous system. J Virol (1998) 2.15

Hrs is associated with STAM, a signal-transducing adaptor molecule. Its suppressive effect on cytokine-induced cell growth. J Biol Chem (1997) 2.03

Activation of natural killer cells via the p75 interleukin 2 receptor. J Exp Med (1989) 1.95

Helper-cytotoxic T lymphocyte (CTL) determinant linkage required for priming of anti-HIV CD8+ CTL in vivo with peptide vaccine constructs. J Immunol (1994) 1.87

Visiting a forest, but not a city, increases human natural killer activity and expression of anti-cancer proteins. Int J Immunopathol Pharmacol (2008) 1.76

Interaction between calcium ions and surface charge as it relates to calcium currents. J Membr Biol (1983) 1.76

Association between Fc epsilon RI beta and atopic disorder in a Japanese population. Lancet (1996) 1.67

Substructure of the thick filament of vertebrate striated muscle. J Mol Biol (1974) 1.61

A forest bathing trip increases human natural killer activity and expression of anti-cancer proteins in female subjects. J Biol Regul Homeost Agents (2008) 1.61

Isolation and composition of thick filaments from rabbit skeletal muscle. J Mol Biol (1973) 1.61

Search for supersymmetry using final states with one lepton, jets, and missing transverse momentum with the ATLAS detector in √s=7 TeV pp collisions. Phys Rev Lett (2011) 1.60

Association between genetic variants of mast-cell chymase and eczema. Lancet (1996) 1.57

Differential expression of the IL-2 receptor subunits, p55 and p75 on various populations of primary peripheral blood mononuclear cells. J Immunol (1989) 1.55

Serum angiotensin-1 converting enzyme activity processes a human immunodeficiency virus 1 gp160 peptide for presentation by major histocompatibility complex class I molecules. J Exp Med (1992) 1.46

The significance of specific IgG4 antibodies to methyltetrahydrophthalic anhydride in occupationally exposed subjects. Clin Exp Allergy (1998) 1.44

Association of ALDH(2) genotypes and alcohol consumption with periodontitis. J Dent Res (2004) 1.43

Observation of a centrality-dependent dijet asymmetry in lead-lead collisions at sqrt[S(NN)] =2.76 TeV with the ATLAS detector at the LHC. Phys Rev Lett (2010) 1.41

Genotypic and phenotypic diversity of rabies virus variants involved in human rabies: implications for postexposure prophylaxis. J Hum Virol (2000) 1.41

Possible involvement of a novel STAM-associated molecule "AMSH" in intracellular signal transduction mediated by cytokines. J Biol Chem (1999) 1.41

Reconstitution of functional interleukin 2 receptor complexes on fibroblastoid cells: involvement of the cytoplasmic domain of the gamma chain in two distinct signaling pathways. Proc Natl Acad Sci U S A (1993) 1.39

Sharing of the IL-2 receptor gamma chain with the functional IL-9 receptor complex. Int Immunol (1995) 1.39

Cryptic dimer interface and domain organization of the extracellular region of metabotropic glutamate receptor subtype 1. J Biol Chem (2000) 1.39

Measurement of dijet azimuthal decorrelations in pp collisions at sqrt(s)=7  TeV. Phys Rev Lett (2011) 1.39

Grf40, A novel Grb2 family member, is involved in T cell signaling through interaction with SLP-76 and LAT. J Exp Med (1999) 1.39

The common gamma-chain for multiple cytokine receptors. Adv Immunol (1995) 1.38

Hgs (Hrs), a FYVE domain protein, is involved in Smad signaling through cooperation with SARA. Mol Cell Biol (2000) 1.37

Propagation of human parvovirus B19 in primary culture of erythroid lineage cells derived from fetal liver. J Virol (1989) 1.37

Evaluation of a simple loop-mediated isothermal amplification test kit for the diagnosis of tuberculosis. Int J Tuberc Lung Dis (2011) 1.37

Healthy lifestyles are associated with higher natural killer cell activity. Prev Med (1992) 1.35

Determination of the strange-quark density of the proton from ATLAS measurements of the W→ℓν and Z→ℓℓ cross sections. Phys Rev Lett (2012) 1.34

A simple method for tissue pressure determination. Arch Surg (1975) 1.34

Forest bathing enhances human natural killer activity and expression of anti-cancer proteins. Int J Immunopathol Pharmacol (2007) 1.34

Behavioral lifestyle and mental health status of Japanese factory workers. Prev Med (1994) 1.33

Search for new particles in two-jet final states in 7 TeV proton-proton collisions with the ATLAS detector at the LHC. Phys Rev Lett (2010) 1.32

The relationship between pregnancy outcome and smooth endoplasmic reticulum clusters in MII human oocytes. Hum Reprod (2004) 1.29

Minimal requirements for peptide mediated activation of CD8+ CTL. Mol Immunol (1994) 1.28

Observation of associated near-side and away-side long-range correlations in sqrt[s(NN)]=5.02 TeV proton-lead collisions with the ATLAS detector. Phys Rev Lett (2013) 1.27

Evidence for structural changes in vertebrate thick filaments induced by calcium. J Mol Biol (1974) 1.26

Activation of peripheral blood T cells via the p75 interleukin 2 receptor. J Exp Med (1989) 1.25

Characterization of the three genotypes of low Km aldehyde dehydrogenase in a Japanese population. Hum Genet (1994) 1.24

Reconstitution of Ca2+-sensitive gelation of actin filaments with filamin, caldesmon and calmodulin. FEBS Lett (1982) 1.22

Excess beta 2 microglobulin promoting functional peptide association with purified soluble class I MHC molecules. Nature (1991) 1.22

Interleukin 2 (IL-2)-induced tyrosine phosphorylation of IL-2 receptor p75. J Exp Med (1990) 1.20

Search for dark matter candidates and large extra dimensions in events with a photon and missing transverse momentum in pp collision data at sqrt[s]=7 TeV with the ATLAS detector. Phys Rev Lett (2013) 1.19

The mouse prostaglandin E receptor EP2 subtype: cloning, expression, and northern blot analysis. FEBS Lett (1995) 1.16

Association of EXT1 and EXT2, hereditary multiple exostoses gene products, in Golgi apparatus. Biochem Biophys Res Commun (2000) 1.15

Occupational asthma from cobalt sensitivity in workers exposed to hard metal dust. Chest (1989) 1.15

The central nervous system inflammatory response to neurotropic virus infection is peroxynitrite dependent. J Immunol (2001) 1.14

Partial purification and properties of urokinase inhibitor from human placenta. J Biochem (1970) 1.14

The effect of prolonged euglycemic hyperinsulinemia on lean body mass after severe burn. Surgery (2002) 1.14

Search for a supersymmetric partner to the top quark in final states with jets and missing transverse momentum at sqrt[s] = 7 TeV with the ATLAS detector. Phys Rev Lett (2012) 1.13

Serum antibodies to carbonic anhydrase IV in patients with autoimmune pancreatitis. Gut (2005) 1.13

The importance of dominant negative effects of amino acid side chain substitution in peptide-MHC molecule interactions and T cell recognition. J Immunol (1993) 1.12

Caldesmon, a calmodulin-binding, F actin-interacting protein, is present in aorta, uterus and platelets. FEBS Lett (1983) 1.11

Overexpression of cytochrome C by a recombinant rabies virus attenuates pathogenicity and enhances antiviral immunity. J Virol (2001) 1.09

Isolation and physical chemical properties of an M-line protein from skeletal muscle. J Biol Chem (1972) 1.09

Exercise and aspirin increase levels of circulating gliadin peptides in patients with wheat-dependent exercise-induced anaphylaxis. Clin Exp Allergy (2005) 1.09

Urokinase inhibitor in human placenta. Nature (1968) 1.08

A review of the genotoxicity of 1-ethyl-1-nitrosourea. Mutat Res (1993) 1.08

STAM2, a new member of the STAM family, binding to the Janus kinases. FEBS Lett (2000) 1.08

IL-2 receptor subunit, p75: direct demonstration of its IL-2 binding ability by using a novel monoclonal antibody. Int Immunol (1989) 1.08

Search for tb resonances in proton-proton collisions at √s=7 TeV with the ATLAS detector. Phys Rev Lett (2012) 1.07

Biotransformation of digitoxigenin by cultured Strophanthus hybrid cells. Phytochemistry (1998) 1.07

Brain magnetic resonance imaging in 23 patients with mucopolysaccharidoses and the effect of bone marrow transplantation. Ann Neurol (2001) 1.05

Cloning and characterization of the Salmonella typhimurium ada gene, which encodes O6-methylguanine-DNA methyltransferase. J Bacteriol (1991) 1.05

Association between atopic asthma and a coding variant of Fc epsilon RI beta in a Japanese population. Hum Mol Genet (1996) 1.05

Dimerization and activity of chymotrypsin at pH4. Biochemistry (1967) 1.05

Cloning of the mouse interleukin 2 receptor gamma chain: demonstration of functional differences between the mouse and human receptors. Biochem Biophys Res Commun (1993) 1.05

[Studies on the physical surface area of Japanese. 18. Calculation formulas in three stages over all ages]. Nihon Eiseigaku Zasshi (1968) 1.05

Search for a light Higgs boson decaying to long-lived weakly interacting particles in proton-proton collisions at sqrt[s] = 7 TeV with the ATLAS detector. Phys Rev Lett (2012) 1.05

Personal health practices and attitudes toward nonsmokers' legal rights in Japan. Soc Sci Med (1991) 1.04

Intramolecular epitope spreading among anti-caspase-8 autoantibodies in patients with silicosis, systemic sclerosis and systemic lupus erythematosus, as well as in healthy individuals. Clin Exp Immunol (2002) 1.03

Progesterone-induced smooth muscle-like cells in the subperitoneal nodules produced by estrogen. Experimental approach to leiomyomatosis peritonealis disseminata. Am J Obstet Gynecol (1981) 1.03

Protein phosphorylation mediated by IL-2/IL-2 receptor beta-chain interaction. J Immunol (1988) 1.03

Actin polymerization induced by calspectin, a calmodulin-binding spectrin-like protein. FEBS Lett (1982) 1.03

Crocidolite asbestos increased 8-hydroxydeoxyguanosine levels in cellular DNA of a human promyelocytic leukemia cell line, HL60. Carcinogenesis (1994) 1.02

[Relationship between working-life satisfaction, health practices and primary symptoms/problems]. Nihon Eiseigaku Zasshi (1991) 1.01

Lifestyle and periodontal health status of Japanese factory workers. Ann Periodontol (1998) 1.01

Increased formation of 8-hydroxydeoxyguanosine, an oxidative DNA damage, in lymphoblasts from Fanconi's anemia patients due to possible catalase deficiency. Carcinogenesis (1993) 1.01

Resource use of insect seed predators during general flowering and seeding events in a Bornean dipterocarp rain forest. Bull Entomol Res (2003) 1.00

Application of surface-coated liposomes for oral delivery of peptide: effects of coating the liposome's surface on the GI transit of insulin. J Pharm Sci (1999) 1.00

Expression of the IL-2 receptor gamma chain on various populations in human peripheral blood. Int Immunol (1994) 1.00

Search for supersymmetry in events with three leptons and missing transverse momentum in √[s]=7 TeV pp collisions with the ATLAS detector. Phys Rev Lett (2012) 0.99

Fixed drug eruption induced by ciprofloxacin followed by ofloxacin. Contact Dermatitis (1994) 0.99

Self-reported alcohol-associated symptoms and drinking behavior in three ALDH2 genotypes among Japanese university students. Alcohol Clin Exp Res (1999) 0.99

Immunohistochemical investigation of cerebral ischemia in gerbils. J Neuropathol Exp Neurol (1985) 0.99

The contribution of polymorphism in the alcohol dehydrogenase beta subunit to alcohol sensitivity in a Japanese population. Hum Genet (1996) 0.98

Mutation of human retinal fascin gene (FSCN2) causes autosomal dominant retinitis pigmentosa. Invest Ophthalmol Vis Sci (2001) 0.98