Published in FEMS Microbiol Lett on June 15, 1990
NADH dehydrogenase defects confer isoniazid resistance and conditional lethality in Mycobacterium smegmatis. J Bacteriol (1998) 2.02
Xylose (glucose) isomerase gene from the thermophile Thermus thermophilus: cloning, sequencing, and comparison with other thermostable xylose isomerases. J Bacteriol (1991) 1.51
Structure of the phenylalanyl-tRNA synthetase genes from Thermus thermophilus HB8 and their expression in Escherichia coli. Nucleic Acids Res (1992) 0.95
Molecular cloning of the pyrE gene from the extreme thermophile Thermus flavus. J Bacteriol (1995) 0.83
Tetrameric malate dehydrogenase from a thermophilic Bacillus: cloning, sequence and overexpression of the gene encoding the enzyme and isolation and characterization of the recombinant enzyme. Biochem J (1996) 0.81
Catalytic-rate improvement of a thermostable malate dehydrogenase by a subtle alteration in cofactor binding. Biochem J (1995) 0.78
The pMTL nic- cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene (1988) 5.38
Improved plasmid vectors for the isolation of translational lac gene fusions. Gene (1984) 4.08
Characterization of a short, cis-acting DNA sequence which conveys cell cycle stage-dependent transcription in Saccharomyces cerevisiae. Mol Cell Biol (1991) 2.91
A specific, highly active malate dehydrogenase by redesign of a lactate dehydrogenase framework. Science (1988) 2.63
Multiple active varieties of Neurospora glutamate dehydrogenase formed by hybridization between two inactive mutant proteins in vivo and in vitro. J Mol Biol (1966) 2.20
A specific endonuclease from Bacillus caldolyticus. Nucleic Acids Res (1978) 2.18
Identification of functional regions in the transforming protein of Fujinami sarcoma virus by in-phase insertion mutagenesis. Cell (1984) 2.12
Recent advances in the genetics of the clostridia. FEMS Microbiol Rev (1989) 2.04
Design and synthesis of new enzymes based on the lactate dehydrogenase framework. Philos Trans R Soc Lond B Biol Sci (1991) 2.02
Resisting and promoting new technologies in clinical practice: the case of telepsychiatry. Soc Sci Med (2001) 1.84
Binding and hydrolysis of nucleotides in the chaperonin catalytic cycle: implications for the mechanism of assisted protein folding. Biochemistry (1993) 1.78
The complete amino acid sequence of the Clostridium botulinum type A neurotoxin, deduced by nucleotide sequence analysis of the encoding gene. Eur J Biochem (1990) 1.78
Cloning, expression and complete nucleotide sequence of the Bacillus stearothermophilus L-lactate dehydrogenase gene. Gene (1986) 1.76
Physical characterisation of the replication region of the Streptococcus faecalis plasmid pAM beta 1. Gene (1990) 1.73
Molecular evolution of bacterial cell-surface proteins. Trends Biochem Sci (1993) 1.72
Anaerobic bacteria as a gene delivery system that is controlled by the tumor microenvironment. Gene Ther (1997) 1.71
Anaerobic bacteria as a delivery system for cancer gene therapy: in vitro activation of 5-fluorocytosine by genetically engineered clostridia. Gene Ther (1996) 1.66
The bacterial oxidation of vitamin B6. VII. Purification, properties, and mechanism of action of an oxygenase which cleaves the 3-hydroxypyridine ring. J Biol Chem (1969) 1.63
A simple procedure for gel electrophoresis and northern blotting of RNA. Nucleic Acids Res (1995) 1.58
The complete nucleotide sequence of the Pseudomonas gene coding for carboxypeptidase G2. Gene (1984) 1.57
Molecular cloning of the Pseudomonas carboxypeptidase G2 gene and its expression in Escherichia coli and Pseudomonas putida. J Bacteriol (1983) 1.54
Molecular cloning of multiple xylanase genes from Pseudomonas fluorescens subsp. cellulosa. J Gen Microbiol (1988) 1.53
Anaplerotic CO2 fixation in mesophilic and thermophilic bacilli. Biochim Biophys Acta (1969) 1.52
Chromosomal location of a gene defining nicotinamide deamidase in Escherichia coli. J Bacteriol (1970) 1.49
Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence. Appl Environ Microbiol (1992) 1.47
Biosynthesis of nicotinamide-adenine dinucleotide in Escherichia coli. Biochim Biophys Acta (1967) 1.45
Site-directed mutagenesis reveals role of mobile arginine residue in lactate dehydrogenase catalysis. Nature (1987) 1.43
The purification of restriction endonuclease EcoRI by precipitation involving polyethyleneimine. FEBS Lett (1977) 1.42
Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production. Metab Eng (2009) 1.41
Anticancer efficacy of systemically delivered anaerobic bacteria as gene therapy vectors targeting tumor hypoxia/necrosis. Gene Ther (2002) 1.37
Identification of the promoter of the Pseudomonas gene coding for carboxypeptidase G2. J Mol Appl Genet (1985) 1.32
Nucleotide sequence of the Erwinia chrysanthemi NCPPB 1066 L-asparaginase gene. Gene (1986) 1.31
The complete amino acid sequence of the Clostridium botulinum type-E neurotoxin, derived by nucleotide-sequence analysis of the encoding gene. Eur J Biochem (1992) 1.30
An investigation of the contribution made by the carboxylate group of an active site histidine-aspartate couple to binding and catalysis in lactate dehydrogenase. Biochemistry (1988) 1.25
Complete nucleotide sequence of the Rhodosporidium toruloides gene coding for phenylalanine ammonia-lyase. Gene (1987) 1.24
Triazine-dye affinity; chromatography. Biochem Soc Trans (1981) 1.24
Malate dehydrogenase from thermophilic and mesophilic bacteria. Molecular size, subunit structure, amino acid composition, immunochemical homology, and catalytic activity. Biochemistry (1980) 1.24
Regeneration of protoplasts of Clostridium pasteurianum ATCC 6013. J Bacteriol (1983) 1.20
Properties and regulation of pyruvate carboxylase from Bacillus stearothermophilus. Proc R Soc Lond B Biol Sci (1970) 1.19
Repeated cycles of Clostridium-directed enzyme prodrug therapy result in sustained antitumour effects in vivo. Br J Cancer (2006) 1.19
The use of genetically engineered tryptophan to identify the movement of a domain of B. stearothermophilus lactate dehydrogenase with the process which limits the steady-state turnover of the enzyme. Biochem Biophys Res Commun (1988) 1.18
A new rapid procedure for the preparation of plasmid DNA. Anal Biochem (1986) 1.17
Physiological role of pyruvate carboxylase in a thermophilic bacillus. J Bacteriol (1973) 1.15
Isolation and partial characterization of four plasmids from antibiotic-resistant thermophilic bacilli. J Gen Microbiol (1979) 1.11
Characterization of a region of the Enterococcus faecalis plasmid pAM beta 1 which enhances the segregational stability of pAM beta 1-derived cloning vectors in Bacillus subtilis. Plasmid (1991) 1.11
Expression of the bacterial nitroreductase enzyme in mammalian cells renders them selectively sensitive to killing by the prodrug CB1954. Eur J Cancer (1995) 1.09
Synthesis of pyruvate carboxylase from its apoenzyme and (+)-biotin in Bacillus stearothermophilus. Purification and properties of the apoenzyme and the holoenzyme synthetase. Biochem J (1971) 1.08
The energetics and cooperativity of protein folding: a simple experimental analysis based upon the solvation of internal residues. Biochemistry (1993) 1.08
Regulation of pyruvate carboxylase formation from the apo-enzyme and biotin in a thermophilic bacillus. Nature (1969) 1.07
Isolation and characterization of isocitrate lyase from a thermophilic Bacillus sp. Biochem J (1978) 1.06
Physical characterisation of the Escherichia coli B gene encoding nitroreductase and its over-expression in Escherichia coli K12. FEMS Microbiol Lett (1994) 1.06
From analysis to synthesis: new ligand binding sites on the lactate dehydrogenase framework. Part II. Trends Biochem Sci (1989) 1.06
Introduction of genes for leucine biosynthesis from Clostridium pasteurianum into C. acetobutylicum by cointegrate conjugal transfer. Mol Gen Genet (1988) 1.05
A strong carboxylate-arginine interaction is important in substrate orientation and recognition in lactate dehydrogenase. Biochim Biophys Acta (1987) 1.05
The replication proteins of plasmids pE194 and pLS1 have N-terminal homology. Nucleic Acids Res (1988) 1.05
Affinity of chaperonin-60 for a protein substrate and its modulation by nucleotides and chaperonin-10. Biochem J (1994) 1.05
Cloning and complete nucleotide sequence of the Bacillus stearothermophilus tryptophanyl tRNA synthetase gene. Gene (1986) 1.04
Transient NMDA receptor inactivation provides long-term protection to cultured cortical neurons from a variety of death signals. J Neurosci (2000) 1.04
Sequential 1H NMR assignments and secondary structure of an IgG-binding domain from protein G. Biochemistry (1991) 1.03
From analysis to synthesis: new ligand binding sites on the lactate dehydrogenase framework. Part I. Trends Biochem Sci (1989) 1.03
Nucleotide sequence analysis of the gene for protein A from Staphylococcus aureus Cowan 1 (NCTC8530) and its enhanced expression in Escherichia coli. Gene (1987) 1.03
The influence of changes in tension on protein synthesis and prostaglandin release in isolated rabbit muscles. Biochem J (1983) 1.03
Genetic characterisation of the botulinum toxin complex of Clostridium botulinum strain NCTC 2916. FEMS Microbiol Lett (1996) 1.02
Affinity labelling of enzymes with triazine dyes. Isolation of a peptide in the catalytic domain of horse-liver alcohol dehydrogenase using Procion blue MX-R as a structural probe. Eur J Biochem (1982) 1.01
The stability and hydrophobicity of cytosolic and mitochondrial malate dehydrogenases and their relation to chaperonin-assisted folding. FEBS Lett (1994) 1.01
Cloning and sequence analysis of the genes encoding phosphotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052. Gene (1993) 1.01
The social costs of unemployment: implications for social support. J Health Soc Behav (1986) 1.00
Nucleotide sequence of the thymidylate synthetase gene (thyP3) from the Bacillus subtilis phage phi 3T. Gene (1985) 1.00
Characterization of Bacillus stearothermophilus plasmid pab124 and construction of deletion variants. J Gen Microbiol (1980) 1.00
Designs for a broad substrate specificity keto acid dehydrogenase. Biochemistry (1990) 1.00
Mechanism of pyruvate carboxylase formation from the apo-enzyme and biotin in a thermophilic bacillus. Nature (1970) 0.98
The importance of arginine 102 for the substrate specificity of Escherichia coli malate dehydrogenase. Biochem Biophys Res Commun (1992) 0.98
Detection and characterization of intermediates in the folding of large proteins by the use of genetically inserted tryptophan probes. Biochemistry (1991) 0.97
Cloning and overexpression of Lactobacillus helveticus D-lactate dehydrogenase gene in Escherichia coli. Eur J Biochem (1992) 0.97
Expression in Escherichia coli of a synthetic gene coding for horse heart myoglobin. Protein Eng (1991) 0.96
Energetics of Bacillus stearothermophilus growth: molar growth yield and temperature effects on growth efficiency. J Bacteriol (1975) 0.96
A gene system for glucitol transport and metabolism in Clostridium beijerinckii NCIMB 8052. Appl Environ Microbiol (1998) 0.95
The effect of lactation length on the circulating concentrations of progesterone and oestradiol in the early weaned sow. Theriogenology (1981) 0.95
Pyruvate carboxylase from a thermophilic Bacillus. Studies on the specificity of activation by acyl derivatives of coenzyme A and on the properties of catalysis in the absence of activator. Biochem J (1978) 0.94
Isolation and characterisation of the glycerol dehydrogenase from Bacillus stearothermophilus. Biochim Biophys Acta (1989) 0.94
Cloning and expression of the Erwinia chrysanthemi asparaginase gene in Escherichia coli and Erwinia carotovora. J Gen Microbiol (1986) 0.94
Development of a transformation and gene reporter system for group II, non-proteolytic Clostridium botulinum type B strains. J Mol Microbiol Biotechnol (2000) 0.93
Simple efficient methods for the isolation of malate dehydrogenase from thermophilic and mesophilic bacteria. Biochem J (1979) 0.93
Enhanced plasma persistence of therapeutic enzymes by coupling to soluble dextran. Biochem J (1977) 0.93
The purification and characterization of glucokinase from the thermophile Bacillus stearothermophilus. Biochem J (1986) 0.90
A single amino acid substitution deregulates a bacterial lactate dehydrogenase and stabilizes its tetrameric structure. Biochim Biophys Acta (1987) 0.90
Erwinia chrysanthemi L-asparaginase: epitope mapping and production of antigenically modified enzymes. Biochem J (1994) 0.90
Modifying an immunogenic epitope on a therapeutic protein: a step towards an improved system for antibody-directed enzyme prodrug therapy (ADEPT). Br J Cancer (2004) 0.89
An outbreak of avian malaria in captive yellowheads/mohua (Mohoua ochrocephala). N Z Vet J (2008) 0.89
Cloning, sequencing, and expression in Escherichia coli of the Clostridium tetanomorphum gene encoding beta-methylaspartase and characterization of the recombinant protein. Biochemistry (1992) 0.89
Myo-inositol catabolism in Salmonella typhimiurium: enzyme repression dependent on growth history of organism. J Gen Microbiol (1972) 0.89