Published in Sensors (Basel) on December 10, 2010
Bioluminescence in the sea. Ann Rev Mar Sci (2010) 3.15
The crystal structure of the photoprotein aequorin at 2.3 A resolution. Nature (2000) 2.77
Cloning and sequence analysis of cDNA for the luminescent protein aequorin. Proc Natl Acad Sci U S A (1985) 2.42
A versatile ELISA-PCR assay for mRNA quantitation from a few cells. Biotechniques (1993) 1.31
Structure of the Ca2+-regulated photoprotein obelin at 1.7 A resolution determined directly from its sulfur substructure. Protein Sci (2000) 1.29
Recombinant obelin: cloning and expression of cDNA purification, and characterization as a calcium indicator. Methods Enzymol (2000) 1.29
Light-emitters involved in the luminescence of coelenterazine. Luminescence (2000) 1.06
Biotechnological applications of bioluminescence and chemiluminescence. Trends Biotechnol (2004) 1.06
Apoptosis in mycobacterium tuberculosis infection in mice exhibiting varied immunopathology. J Pathol (2000) 0.98
Two excited states in aequorin bioluminescence induced by tryptophan modification. FEBS Lett (1992) 0.95
Obelin from the bioluminescent marine hydroid Obelia geniculata: cloning, expression, and comparison of some properties with those of other Ca2+-regulated photoproteins. Biochemistry (2002) 0.94
Red-shifted aequorin-based bioluminescent reporters for in vivo imaging of Ca2 signaling. Mol Imaging (2007) 0.94
Luminometry: a novel bioluminescent immunoassay enhances the quantitation of mucosal and systemic antibody responses. J Immunol Methods (1996) 0.93
Sequence of the cDNA encoding the Ca(2+)-activated photoprotein obelin from the hydroid polyp Obelia longissima. Gene (1995) 0.92
Aequorin variants with improved bioluminescence properties. Protein Eng Des Sel (2009) 0.90
Spectral tuning of photoproteins by partnering site-directed mutagenesis strategies with the incorporation of chromophore analogs. Protein Eng Des Sel (2008) 0.90
Bioluminescence immunoassay for cortisol using recombinant aequorin as a label. Anal Biochem (2002) 0.89
Quantitative, competitive PCR assay for HIV-1 using a microplate-based detection system. Biotechniques (1998) 0.89
Engineering bioluminescent proteins: expanding their analytical potential. Anal Chem (2009) 0.86
Bacterial expression of in vivo-biotinylated aequorin for direct application to bioluminometric hybridization assays. Anal Biochem (2002) 0.84
Violet bioluminescence and fast kinetics from W92F obelin: structure-based proposals for the bioluminescence triggering and the identification of the emitting species. Biochemistry (2003) 0.84
A flash-type bioluminescent immunoassay that is more sensitive than radioimaging: quantitative detection of cytokine cDNA in activated and resting human cells. J Immunol Methods (1998) 0.83
Cytokine mRNA expression and serum cortisol evaluation during murine lung inflammation induced by Mycobacterium tuberculosis. Comb Chem High Throughput Screen (2000) 0.83
An immunoassay for Leu-enkephalin based on a C-terminal aequorin-peptide fusion. Anal Chem (2001) 0.82
High-level expression and purification of apoaequorin. Protein Expr Purif (1992) 0.82
Aequorin-based homogeneous cortisol immunoassay for analysis of saliva samples. Bioconjug Chem (2007) 0.81
Evaluation of electrochemiluminescence- and bioluminescence-based assays for quantitating specific DNA. J Clin Lab Anal (1996) 0.81
Spectral tuning of obelin bioluminescence by mutations of Trp92. FEBS Lett (2003) 0.81
Application of a liposomal bioluminescent label in the development of a flow injection immunoanalytical system. Anal Chem (2005) 0.81
Quantitation of Chlamydia trachomatis 16S rRNA using NASBA amplification and a bioluminescent microtiter plate assay. Comb Chem High Throughput Screen (2000) 0.80
Quantitative and bioluminescent assay to measure efficacy of conventional and DNA vaccinations against Helicobacter pylori. Comb Chem High Throughput Screen (2000) 0.80
Bioluminescent immunoassay using a fusion protein of protein A and the photoprotein aequorin. Biochem Biophys Res Commun (1990) 0.79
A flexible bioluminescent-quantitative polymerase chain reaction assay for analysis of competitive PCR amplicons. J Clin Lab Anal (1999) 0.79
Competitive RT-PCR ELISA: a rapid, sensitive and non-radioactive method to quantitate cytokine mRNA. J Immunol Methods (1994) 0.79
Bioluminescence DNA hybridization assay for Plasmodium falciparum based on the photoprotein aequorin. Anal Chem (2007) 0.78
C-terminal and n-terminal fusions of aequorin with small peptides in immunoassay development. Bioconjug Chem (2001) 0.78
Determination of prostacyclin in plasma through a bioluminescent immunoassay for 6-keto-prostaglandin F1alpha: implication of dosage in patients with primary pulmonary hypertension. Anal Chem (2002) 0.78
Bioluminescent immunoassay of thyrotropin and thyroxine using obelin as a label. Anal Biochem (2004) 0.78
Use of photoproteins as intracellular calcium indicators. Environ Health Perspect (1990) 0.78
Recombinant aequorin with a reactive cysteine residue for conjugation with maleimide-activated antibody. Anal Biochem (2008) 0.78
Modulating the bioluminescence emission of photoproteins by in vivo site-directed incorporation of non-natural amino acids. ACS Chem Biol (2010) 0.78
Expression and secretion of aequorin as a chimeric antibody by means of a mammalian expression vector. Proc Natl Acad Sci U S A (1990) 0.78
Quantitation of RT-PCR amplified cytokine mRNA by aequorin-based bioluminescence immunoassay. J Immunol Methods (1996) 0.77
Patterns of expression of viral and cytokine gene transcripts during mouse polyoma virus infection. Comb Chem High Throughput Screen (2000) 0.77
Violet and greenish photoprotein obelin mutants for reporter applications in dual-color assay. Anal Bioanal Chem (2008) 0.77
Genetically modified semisynthetic bioluminescent photoprotein variants: simultaneous dual-analyte assay in a single well employing time resolution of decay kinetics. Anal Chem (2008) 0.77
Highly sensitive and rapid tandem bioluminescent immunoassay using aequorin labeled Fab fragment and biotinylated firefly luciferase. Anal Chim Acta (2007) 0.77
Barnase-barstar high affinity interaction phenomenon as the base for the heterogenous bioluminescence pseudorabies virus' immunoassay. J Biochem Biophys Methods (2007) 0.76
Quantitation of polymerase chain reaction products by hybridization-based assays with fluorescent, colorimetric, or chemiluminescent detection. Anal Biochem (1992) 0.76
Bioluminescent signal system: bioluminescence immunoassay of pathogenic organisms. Luminescence (2007) 0.76
Quantification of prostate-specific antigen mRNA by coamplification with a recombinant RNA internal standard and microtiter well-based hybridization. Clin Chem (1998) 0.76
Dual analyte detection using tandem flash luminescence. Bioorg Med Chem Lett (2002) 0.76
Quadruple-allele chemiluminometric assay for simultaneous genotyping of two single-nucleotide polymorphisms. Analyst (2009) 0.76
[Conjugates of the Ca2+ -regulated photoprotein obelin with immunoglobulins: synthesis and use as labels in bioluminescent immunoassay]. Bioorg Khim (2004) 0.76
Site-specifically labeled photoprotein-thyroxine conjugates using aequorin mutants containing unique cysteine residues: applications for binding assays (Part II). Bioconjug Chem (2000) 0.76
High-throughput double quantitative competitive polymerase chain reaction for determination of genetically modified organisms. Anal Chem (2005) 0.76
Use of recombinant biotinylated aequorin in microtiter and membrane-based assays: purification of recombinant apoaequorin from Escherichia coli. Biochemistry (1992) 0.76
Use of proZZ-obelin fusion protein in bioluminescent immunoassay. Biochem Biophys Res Commun (1996) 0.76
[A highly sensitive and rapid method for the detection of DNA fragments using the photoprotein obelin as a reporter]. Bioorg Khim (2008) 0.76
Bioluminescent quantitation and detection of gene expression during infectious disease. Comb Chem High Throughput Screen (2000) 0.76
Bioluminescent immunoassay using a monomeric Fab'-photoprotein aequorin conjugate. Biochem Biophys Res Commun (1991) 0.76
AquaLite bioluminescence assay of thyrotropin in serum evaluated. Clin Chem (1995) 0.75