High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire.

PubWeight™: 2.81‹?› | Rank: Top 1%

🔗 View Article (PMC 3910347)

Published in Nat Biotechnol on January 20, 2013

Authors

Brandon J DeKosky1, Gregory C Ippolito, Ryan P Deschner, Jason J Lavinder, Yariv Wine, Brandon M Rawlings, Navin Varadarajan, Claudia Giesecke, Thomas Dörner, Sarah F Andrews, Patrick C Wilson, Scott P Hunicke-Smith, C Grant Willson, Andrew D Ellington, George Georgiou

Author Affiliations

1: Department of Chemical Engineering, University of Texas at Austin, Austin, Texas, USA.

Articles citing this

Single-cell sequencing-based technologies will revolutionize whole-organism science. Nat Rev Genet (2013) 4.03

Developmental pathway for potent V1V2-directed HIV-neutralizing antibodies. Nature (2014) 3.73

The promise and challenge of high-throughput sequencing of the antibody repertoire. Nat Biotechnol (2014) 2.54

Human responses to influenza vaccination show seroconversion signatures and convergent antibody rearrangements. Cell Host Microbe (2014) 1.57

Identification and characterization of the constituent human serum antibodies elicited by vaccination. Proc Natl Acad Sci U S A (2014) 1.50

Personalized, sequencing-based immune profiling spurs startups. Nat Biotechnol (2013) 1.44

Vidjil: A Web Platform for Analysis of High-Throughput Repertoire Sequencing. PLoS One (2016) 1.39

High-resolution antibody dynamics of vaccine-induced immune responses. Proc Natl Acad Sci U S A (2014) 1.39

The past, present, and future of immune repertoire biology - the rise of next-generation repertoire analysis. Front Immunol (2013) 1.22

Beyond model antigens: high-dimensional methods for the analysis of antigen-specific T cells. Nat Biotechnol (2014) 1.22

Sequencing the functional antibody repertoire--diagnostic and therapeutic discovery. Nat Rev Rheumatol (2014) 1.20

Sequence analysis of T-cell repertoires in health and disease. Genome Med (2013) 1.17

Unifying immunology with informatics and multiscale biology. Nat Immunol (2014) 1.15

Barcode-enabled sequencing of plasmablast antibody repertoires in rheumatoid arthritis. Arthritis Rheumatol (2014) 1.14

High-throughput sequencing of natively paired antibody chains provides evidence for original antigenic sin shaping the antibody response to influenza vaccination. Clin Immunol (2014) 1.12

The shape of the lymphocyte receptor repertoire: lessons from the B cell receptor. Front Immunol (2013) 1.07

De novo identification of VRC01 class HIV-1-neutralizing antibodies by next-generation sequencing of B-cell transcripts. Proc Natl Acad Sci U S A (2013) 1.06

Identification of antigen-specific B cell receptor sequences using public repertoire analysis. J Immunol (2014) 1.06

Characterizing immune repertoires by high throughput sequencing: strategies and applications. Trends Immunol (2014) 1.03

Scalable microfluidics for single-cell RNA printing and sequencing. Genome Biol (2015) 1.02

A capture-sequencing strategy identifies IRF8, EBF1, and APRIL as novel IGH fusion partners in B-cell lymphoma. Blood (2013) 1.00

Antibody phage display: technique and applications. J Invest Dermatol (2014) 1.00

Practical guidelines for B-cell receptor repertoire sequencing analysis. Genome Med (2015) 0.99

HIV broadly neutralizing antibody targets. Curr Opin HIV AIDS (2015) 0.98

Single-cell and deep sequencing of IgG-switched macaque B cells reveal a diverse Ig repertoire following immunization. J Immunol (2014) 0.96

In-Depth Assessment of Within-Individual and Inter-Individual Variation in the B Cell Receptor Repertoire. Front Immunol (2015) 0.95

The origin of CDR H3 structural diversity. Structure (2015) 0.94

Designing vaccines for the twenty-first century society. Front Immunol (2014) 0.92

Impact of new sequencing technologies on studies of the human B cell repertoire. Curr Opin Immunol (2013) 0.92

Quantitative assessment of the robustness of next-generation sequencing of antibody variable gene repertoires from immunized mice. BMC Immunol (2014) 0.91

The application of next generation sequencing to the understanding of antibody repertoires. Front Immunol (2013) 0.90

Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput. Nat Methods (2017) 0.90

Large-scale sequence and structural comparisons of human naive and antigen-experienced antibody repertoires. Proc Natl Acad Sci U S A (2016) 0.90

Simulation of B cell affinity maturation explains enhanced antibody cross-reactivity induced by the polyvalent malaria vaccine AMA1. J Immunol (2014) 0.89

Analysis of B Cell Repertoire Dynamics Following Hepatitis B Vaccination in Humans, and Enrichment of Vaccine-specific Antibody Sequences. EBioMedicine (2015) 0.89

The Diversity and Molecular Evolution of B-Cell Receptors during Infection. Mol Biol Evol (2016) 0.89

Accurate and predictive antibody repertoire profiling by molecular amplification fingerprinting. Sci Adv (2016) 0.89

Identifying functional anti-Staphylococcus aureus antibodies by sequencing antibody repertoires of patient plasmablasts. Clin Immunol (2014) 0.88

A bioinformatic framework for immune repertoire diversity profiling enables detection of immunological status. Genome Med (2015) 0.86

SORTCERY-A High-Throughput Method to Affinity Rank Peptide Ligands. J Mol Biol (2014) 0.86

Quantifying evolutionary constraints on B-cell affinity maturation. Philos Trans R Soc Lond B Biol Sci (2015) 0.85

High-throughput sequencing of B- and T-lymphocyte antigen receptors in hematology. Blood (2013) 0.85

Interrogating the relationship between naïve and immune antiviral T cell repertoires. Curr Opin Virol (2013) 0.85

Current status and recent advances of next generation sequencing techniques in immunological repertoire. Genes Immun (2016) 0.84

Proteomic identification of monoclonal antibodies from serum. Anal Chem (2014) 0.84

A population biological approach to understanding the maintenance and loss of the T-cell repertoire during aging. Immunology (2014) 0.83

Advances in antiviral vaccine development. Immunol Rev (2013) 0.82

Direct high-throughput amplification and sequencing of immunoglobulin genes from single human B cells. Eur J Immunol (2015) 0.82

Error analysis of deep sequencing of phage libraries: peptides censored in sequencing. Comput Math Methods Med (2013) 0.82

Systematic characterization and comparative analysis of the rabbit immunoglobulin repertoire. PLoS One (2014) 0.82

Serology in the 21st century: the molecular-level analysis of the serum antibody repertoire. Curr Opin Immunol (2015) 0.82

Comprehensive analysis of the T-cell receptor beta chain gene in rhesus monkey by high throughput sequencing. Sci Rep (2015) 0.81

IgE-associated IGHV genes from venom and peanut allergic individuals lack mutational evidence of antigen selection. PLoS One (2014) 0.81

A Public Database of Memory and Naive B-Cell Receptor Sequences. PLoS One (2016) 0.80

Adaptive Immunity and Antigen-Specific Activation in Obesity-Associated Insulin Resistance. Mediators Inflamm (2015) 0.80

Deep sequencing in library selection projects: what insight does it bring? Curr Opin Struct Biol (2015) 0.80

sciReptor: analysis of single-cell level immunoglobulin repertoires. BMC Bioinformatics (2016) 0.80

Facile Discovery of a Diverse Panel of Anti-Ebola Virus Antibodies by Immune Repertoire Mining. Sci Rep (2015) 0.79

Deep sequencing and human antibody repertoire analysis. Curr Opin Immunol (2016) 0.78

Rhesus Macaque B-Cell Responses to an HIV-1 Trimer Vaccine Revealed by Unbiased Longitudinal Repertoire Analysis. MBio (2015) 0.78

Hybridization-based antibody cDNA recovery for the production of recombinant antibodies identified by repertoire sequencing. MAbs (2013) 0.78

Tissue-specific expressed antibody variable gene repertoires. PLoS One (2014) 0.78

Next-Generation Sequencing Reveals Restriction and Clonotypic Expansion of Treg Cells in Juvenile Idiopathic Arthritis. Arthritis Rheumatol (2016) 0.77

Likelihood-Based Inference of B Cell Clonal Families. PLoS Comput Biol (2016) 0.77

Old questions, new tools: does next-generation sequencing hold the key to unraveling intestinal B-cell responses? Mucosal Immunol (2014) 0.77

Rapid and continuous magnetic separation in droplet microfluidic devices. Lab Chip (2015) 0.77

Deep sequencing methods for protein engineering and design. Curr Opin Struct Biol (2016) 0.76

Statistical inference of a convergent antibody repertoire response to influenza vaccine. Genome Med (2016) 0.76

Accurate Structure Prediction of CDR H3 Loops Enabled by a Novel Structure-Based C-Terminal Constraint. J Immunol (2016) 0.76

Predominant structural configuration of natural antibody repertoires enables potent antibody responses against protein antigens. Sci Rep (2015) 0.76

Unique Features of Fish Immune Repertoires: Particularities of Adaptive Immunity Within the Largest Group of Vertebrates. Results Probl Cell Differ (2015) 0.76

High-Throughput Sequencing-Based Immune Repertoire Study during Infectious Disease. Front Immunol (2016) 0.76

Platform for high-throughput antibody selection using synthetically-designed antibody libraries. N Biotechnol (2015) 0.76

Antibodyomics: bioinformatics technologies for understanding B-cell immunity to HIV-1. Immunol Rev (2017) 0.76

Immune Repertoire Diversity Correlated with Mortality in Avian Influenza A (H7N9) Virus Infected Patients. Sci Rep (2016) 0.75

Multiplexed Affinity-Based Separation of Proteins and Cells Using Inertial Microfluidics. Sci Rep (2016) 0.75

Comparative Analysis of Immune Repertoires between Bactrian Camel's Conventional and Heavy-Chain Antibodies. PLoS One (2016) 0.75

Discovery of high affinity anti-ricin antibodies by B cell receptor sequencing and by yeast display of combinatorial VH:VL libraries from immunized animals. MAbs (2016) 0.75

High-throughput sequencing of immune repertoires in multiple sclerosis. Ann Clin Transl Neurol (2016) 0.75

Generating High-Accuracy Peptide-Binding Data in High Throughput with Yeast Surface Display and SORTCERY. Methods Mol Biol (2016) 0.75

BRILIA: Integrated Tool for High-Throughput Annotation and Lineage Tree Assembly of B-Cell Repertoires. Front Immunol (2017) 0.75

VDJviz: a versatile browser for immunogenomics data. BMC Genomics (2016) 0.75

Temporal stability and molecular persistence of the bone marrow plasma cell antibody repertoire. Nat Commun (2016) 0.75

Scaling by shrinking: empowering single-cell 'omics' with microfluidic devices. Nat Rev Genet (2017) 0.75

Identifying T Cell Receptors from High-Throughput Sequencing: Dealing with Promiscuity in TCRα and TCRβ Pairing. PLoS Comput Biol (2017) 0.75

The Cloning and Expression of Human Monoclonal Antibodies: Implications for Allergen Immunotherapy. Curr Allergy Asthma Rep (2016) 0.75

Robust estimates of overall immune-repertoire diversity from high-throughput measurements on samples. Nat Commun (2016) 0.75

Improving the developability of an anti-EphA2 single-chain variable fragment for nanoparticle targeting. MAbs (2016) 0.75

Investigating the effect of AS03 adjuvant on the plasma cell repertoire following pH1N1 influenza vaccination. Sci Rep (2016) 0.75

Single-cell TCRseq: paired recovery of entire T-cell alpha and beta chain transcripts in T-cell receptors from single-cell RNAseq. Genome Med (2016) 0.75

Progress Report on the Generation of Polyfunctional Microscale Particles for Programmed Self-Assembly. Chem Mater (2014) 0.75

Sequence analysis of feline immunoglobulin mRNAs and the development of a felinized monoclonal antibody specific to feline panleukopenia virus. Sci Rep (2017) 0.75

Novel in vitro booster vaccination to rapidly generate antigen-specific human monoclonal antibodies. J Exp Med (2017) 0.75

Assessment of antibody library diversity through next generation sequencing and technical error compensation. PLoS One (2017) 0.75

Accurate immune repertoire sequencing reveals malaria infection driven antibody lineage diversification in young children. Nat Commun (2017) 0.75

A Haystack Heuristic for Autoimmune Disease Biomarker Discovery Using Next-Gen Immune Repertoire Sequencing Data. Sci Rep (2017) 0.75

Using high-throughput barcode sequencing to efficiently map connectomes. Nucleic Acids Res (2017) 0.75

Articles cited by this

Predominant autoantibody production by early human B cell precursors. Science (2003) 13.14

Rapid cloning of high-affinity human monoclonal antibodies against influenza virus. Nature (2008) 10.80

Sequence and structural convergence of broad and potent HIV antibodies that mimic CD4 binding. Science (2011) 9.00

Focused evolution of HIV-1 neutralizing antibodies revealed by structures and deep sequencing. Science (2011) 7.92

IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis. Nucleic Acids Res (2008) 6.91

Measurements of the true affinity constant in solution of antigen-antibody complexes by enzyme-linked immunosorbent assay. J Immunol Methods (1985) 6.11

High-throughput sequencing of the zebrafish antibody repertoire. Science (2009) 4.46

Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen. Nat Protoc (2009) 3.30

Pandemic H1N1 influenza vaccine induces a recall response in humans that favors broadly cross-reactive memory B cells. Proc Natl Acad Sci U S A (2012) 3.27

High-throughput microfluidic single-cell RT-qPCR. Proc Natl Acad Sci U S A (2011) 2.57

A proteomics approach for the identification and cloning of monoclonal antibodies from serum. Nat Biotechnol (2012) 2.51

Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells. Nat Biotechnol (2010) 2.41

Isolation of human antibody repertoires with preservation of the natural heavy and light chain pairing. J Mol Biol (2006) 2.05

Rep-Seq: uncovering the immunological repertoire through next-generation sequencing. Immunology (2012) 1.99

IMGT/3Dstructure-DB and IMGT/DomainGapAlign: a database and a tool for immunoglobulins or antibodies, T cell receptors, MHC, IgSF and MhcSF. Nucleic Acids Res (2009) 1.94

Blood-borne human plasma cells in steady state are derived from mucosal immune responses. Blood (2008) 1.91

Naive antibody gene-segment frequencies are heritable and unaltered by chronic lymphocyte ablation. Proc Natl Acad Sci U S A (2011) 1.74

Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns. Nat Protoc (2012) 1.53

Sequencing antibody repertoires: the next generation. MAbs (2011) 1.52

Tools to therapeutically harness the human antibody response. Nat Rev Immunol (2012) 1.30

Frequent occurrence of identical heavy and light chain Ig rearrangements. Int Immunol (1997) 1.29

Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cells. J Immunol Methods (2008) 1.28

Antibody internalization studied using a novel IgG binding toxin fusion. J Immunol Methods (2007) 1.26

Secondary immunization generates clonally related antigen-specific plasma cells and memory B cells. J Immunol (2010) 1.20

Differential regulation of self-reactivity discriminates between IgG+ human circulating memory B cells and bone marrow plasma cells. Proc Natl Acad Sci U S A (2011) 1.14

Antibody repertoires in humanized NOD-scid-IL2Rγ(null) mice and human B cells reveals human-like diversification and tolerance checkpoints in the mouse. PLoS One (2012) 1.09

Proteomics-directed cloning of circulating antiviral human monoclonal antibodies. Nat Biotechnol (2012) 1.08

Systems analysis of adaptive immunity by utilization of high-throughput technologies. Curr Opin Biotechnol (2011) 0.97

Single-cell analysis of T-cell receptor repertoire of HTLV-1 Tax-specific cytotoxic T cells in allogeneic transplant recipients with adult T-cell leukemia/lymphoma. Cancer Res (2010) 0.92

V-gene amplification revisited - An optimised procedure for amplification of rearranged human antibody genes of different isotypes. N Biotechnol (2010) 0.91

Articles by these authors

Rapid cloning of high-affinity human monoclonal antibodies against influenza virus. Nature (2008) 10.80

Broadly cross-reactive antibodies dominate the human B cell response against 2009 pandemic H1N1 influenza virus infection. J Exp Med (2011) 7.29

Competence and competition: the challenge of becoming a long-lived plasma cell. Nat Rev Immunol (2006) 5.22

Synthetic biology: engineering Escherichia coli to see light. Nature (2005) 4.58

New approaches to nanofabrication: molding, printing, and other techniques. Chem Rev (2005) 4.33

Polyreactivity increases the apparent affinity of anti-HIV antibodies by heteroligation. Nature (2010) 3.96

Isolation of engineered, full-length antibodies from libraries expressed in Escherichia coli. Nat Biotechnol (2007) 3.56

Developmental and species-divergent globin switching are driven by BCL11A. Nature (2009) 3.49

Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen. Nat Protoc (2009) 3.30

Pandemic H1N1 influenza vaccine induces a recall response in humans that favors broadly cross-reactive memory B cells. Proc Natl Acad Sci U S A (2012) 3.27

A synthetic genetic edge detection program. Cell (2009) 3.20

Generation of migratory antigen-specific plasma blasts and mobilization of resident plasma cells in a secondary immune response. Blood (2004) 3.17

Virus-based toolkit for the directed synthesis of magnetic and semiconducting nanowires. Science (2004) 3.08

Protection against anthrax toxin by recombinant antibody fragments correlates with antigen affinity. Nat Biotechnol (2002) 2.77

Aptamer mediated siRNA delivery. Nucleic Acids Res (2006) 2.77

Lineage structure of the human antibody repertoire in response to influenza vaccination. Sci Transl Med (2013) 2.71

Applications of aptamers as sensors. Annu Rev Anal Chem (Palo Alto Calif) (2009) 2.45

Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells. Nat Biotechnol (2010) 2.41

Widespread reorganization of metabolic enzymes into reversible assemblies upon nutrient starvation. Proc Natl Acad Sci U S A (2009) 2.39

Correction of sickle cell disease in adult mice by interference with fetal hemoglobin silencing. Science (2011) 2.36

Correlation between circulating CD27high plasma cells and disease activity in patients with systemic lupus erythematosus. Arthritis Rheum (2003) 2.32

Folding quality control in the export of proteins by the bacterial twin-arginine translocation pathway. Proc Natl Acad Sci U S A (2003) 2.26

Aptamer:toxin conjugates that specifically target prostate tumor cells. Cancer Res (2006) 2.23

Joint European League Against Rheumatism and European Renal Association-European Dialysis and Transplant Association (EULAR/ERA-EDTA) recommendations for the management of adult and paediatric lupus nephritis. Ann Rheum Dis (2012) 2.21

Human immunoglobulin selection associated with class switch and possible tolerogenic origins for C delta class-switched B cells. J Clin Invest (2004) 2.21

The many faces of glutathione in bacteria. Antioxid Redox Signal (2006) 2.20

Mature B cells class switched to IgD are autoreactive in healthy individuals. J Clin Invest (2007) 2.18

The bacterial twin-arginine translocation pathway. Annu Rev Microbiol (2006) 2.13

Efficacy and safety of rituximab treatment in patients with antineutrophil cytoplasmic antibody-associated vasculitides: results from a German registry (GRAID). J Rheumatol (2012) 2.12

Marburg I polymorphism of factor VII-activating protease is associated with idiopathic venous thromboembolism. Blood (2004) 2.05

Bacterial biosynthesis of cadmium sulfide nanocrystals. Chem Biol (2004) 2.02

Protein-dependent ribozymes report molecular interactions in real time. Nat Biotechnol (2002) 2.00

Viral assembly of oriented quantum dot nanowires. Proc Natl Acad Sci U S A (2003) 2.00

Activated memory B cell subsets correlate with disease activity in systemic lupus erythematosus: delineation by expression of CD27, IgD, and CD95. Arthritis Rheum (2008) 2.00

Memory B and memory plasma cells. Immunol Rev (2010) 1.95

Limited efficacy of inactivated influenza vaccine in elderly individuals is associated with decreased production of vaccine-specific antibodies. J Clin Invest (2011) 1.94

Updated consensus statement on the use of rituximab in patients with rheumatoid arthritis. Ann Rheum Dis (2011) 1.93

Blood-borne human plasma cells in steady state are derived from mucosal immune responses. Blood (2008) 1.91

Functional anergy in a subpopulation of naive B cells from healthy humans that express autoreactive immunoglobulin receptors. J Exp Med (2008) 1.88

Intricate targeting of immunoglobulin somatic hypermutation maximizes the efficiency of affinity maturation. J Exp Med (2005) 1.85

Long-lived autoreactive plasma cells drive persistent autoimmune inflammation. Nat Rev Rheumatol (2011) 1.82

In-depth determination and analysis of the human paired heavy- and light-chain antibody repertoire. Nat Med (2014) 1.81

Circulating proteasomes are markers of cell damage and immunologic activity in autoimmune diseases. J Rheumatol (2002) 1.80

Rapid and massive virus-specific plasmablast responses during acute dengue virus infection in humans. J Virol (2012) 1.74

Genetic analysis of the twin arginine translocator secretion pathway in bacteria. J Biol Chem (2002) 1.74

FOXP1: a potential therapeutic target in cancer. Expert Opin Ther Targets (2007) 1.72

Plasma cell differentiation and survival. Curr Opin Immunol (2008) 1.71

Aptamer therapeutics advance. Curr Opin Chem Biol (2006) 1.69

Ribozyme catalysis of metabolism in the RNA world. Chem Biodivers (2007) 1.66

Involvement of inducible costimulator in the exaggerated memory B cell and plasma cell generation in systemic lupus erythematosus. Arthritis Rheum (2004) 1.66

Dysregulation of chemokine receptor expression and function by B cells of patients with primary Sjögren's syndrome. Arthritis Rheum (2005) 1.65

Anti-CD20 therapy in patients with rheumatoid arthritis: predictors of response and B cell subset regeneration after repeated treatment. Arthritis Rheum (2008) 1.64

Immunopathogenic mechanisms of systemic autoimmune disease. Lancet (2013) 1.63

Aptamer database. Nucleic Acids Res (2004) 1.60

Inhibition of cell proliferation by an anti-EGFR aptamer. PLoS One (2011) 1.59

Evolutionarily repurposed networks reveal the well-known antifungal drug thiabendazole to be a novel vascular disrupting agent. PLoS Biol (2012) 1.58

Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab. Blood (2010) 1.57

Characterization of a late transitional B cell population highly sensitive to BAFF-mediated homeostatic proliferation. J Exp Med (2008) 1.55

Acute intravascular hemolysis after transfusion of a chimeric RBC unit. Transfusion (2003) 1.55

Anchored periplasmic expression, a versatile technology for the isolation of high-affinity antibodies from Escherichia coli-expressed libraries. Proc Natl Acad Sci U S A (2004) 1.54

Mechanisms of B cell autoimmunity in SLE. Arthritis Res Ther (2011) 1.53

Patterns of spinal sensory-motor connectivity prescribed by a dorsoventral positional template. Cell (2011) 1.51

Detection of anthrax toxin in the serum of animals infected with Bacillus anthracis by using engineered immunoassays. Clin Vaccine Immunol (2006) 1.50

Direct measurement of the reaction front in chemically amplified photoresists. Science (2002) 1.50

Identification and characterization of the constituent human serum antibodies elicited by vaccination. Proc Natl Acad Sci U S A (2014) 1.50

Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods. Nucleic Acids Res (2011) 1.48

Functional studies of BCL11A: characterization of the conserved BCL11A-XL splice variant and its interaction with BCL6 in nuclear paraspeckles of germinal center B cells. Mol Cancer (2006) 1.48

Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity. Proc Natl Acad Sci U S A (2005) 1.48

Immune history shapes specificity of pandemic H1N1 influenza antibody responses. J Exp Med (2013) 1.47

Why high-error-rate random mutagenesis libraries are enriched in functional and improved proteins. J Mol Biol (2005) 1.47

Diminished peripheral blood memory B cells and accumulation of memory B cells in the salivary glands of patients with Sjögren's syndrome. Arthritis Rheum (2002) 1.47

Effects of codon usage versus putative 5'-mRNA structure on the expression of Fusarium solani cutinase in the Escherichia coli cytoplasm. Protein Expr Purif (2003) 1.46

Immunopathogenesis of primary Sjögren's syndrome: implications for disease management and therapy. Curr Opin Rheumatol (2005) 1.46

DNA hybridization and discrimination of single-nucleotide mismatches using chip-based microbead arrays. Anal Chem (2003) 1.46

Modulating the innate immune response by combinatorial engineering of endotoxin. Proc Natl Acad Sci U S A (2013) 1.45

Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1. Proc Natl Acad Sci U S A (2007) 1.45

Treat-to-target in systemic lupus erythematosus: recommendations from an international task force. Ann Rheum Dis (2014) 1.45

Small RNA profiling reveals antisense transcription throughout the KSHV genome and novel small RNAs. RNA (2010) 1.44

Isolation and expression of recombinant antibody fragments to the biological warfare pathogen Brucella melitensis. J Immunol Methods (2003) 1.44

High abundance of plasma cells secreting transglutaminase 2-specific IgA autoantibodies with limited somatic hypermutation in celiac disease intestinal lesions. Nat Med (2012) 1.44

Antibody pressure by a human monoclonal antibody targeting the 2009 pandemic H1N1 virus hemagglutinin drives the emergence of a virus with increased virulence in mice. MBio (2012) 1.43

Foxp1 is an essential transcriptional regulator for the generation of quiescent naive T cells during thymocyte development. Blood (2009) 1.42

Detecting lifetime alcohol problems in individuals referred for liver transplantation for nonalcoholic liver failure. Liver Transpl (2008) 1.40

Probing prokaryotic social behaviors with bacterial "lobster traps". MBio (2010) 1.39

High-resolution antibody dynamics of vaccine-induced immune responses. Proc Natl Acad Sci U S A (2014) 1.39

A serologic marker for fetal risk of congenital heart block. Arthritis Rheum (2002) 1.38

Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates. Biosens Bioelectron (2006) 1.38

Human antibody against shiga toxin 2 administered to piglets after the onset of diarrhea due to Escherichia coli O157:H7 prevents fatal systemic complications. Infect Immun (2005) 1.38

Aptamer-based sensor arrays for the detection and quantitation of proteins. Anal Chem (2004) 1.37

EULAR Sjogren's Syndrome Patient Reported Index (ESSPRI): development of a consensus patient index for primary Sjogren's syndrome. Ann Rheum Dis (2011) 1.37

Selection of fluorescent aptamer beacons that light up in the presence of zinc. Anal Bioanal Chem (2007) 1.37