Transpositional transgenesis with piggyBac.

PubWeight™: 0.82‹?›

🔗 View Article (PMID 23956948)

Published in Mob Genet Elements on May 24, 2013

Authors

Johann Urschitz1, Stefan Moisyadi

Author Affiliations

1: Department of Anatomy, Biochemistry and Physiology; John A. Burns School of Medicine; Honolulu, HI USA.

Articles cited by this

Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science (2002) 17.71

Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell (1997) 8.68

Efficient transposition of the piggyBac (PB) transposon in mammalian cells and mice. Cell (2005) 7.29

Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci U S A (1980) 5.47

Chromosomal transposition of PiggyBac in mouse embryonic stem cells. Proc Natl Acad Sci U S A (2008) 4.98

piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells. Proc Natl Acad Sci U S A (2006) 3.35

Generation of an inducible and optimized piggyBac transposon system. Nucleic Acids Res (2007) 3.09

PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice. Science (2010) 2.31

Mammalian transgenesis by intracytoplasmic sperm injection. Science (1999) 2.28

A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes. J Bacteriol (1997) 1.62

Mobilization of giant piggyBac transposons in the mouse genome. Nucleic Acids Res (2011) 1.49

Lentiviral vectors: are they the future of animal transgenesis? Physiol Genomics (2007) 1.42

Helper-independent piggyBac plasmids for gene delivery approaches: strategies for avoiding potential genotoxic effects. Proc Natl Acad Sci U S A (2010) 1.25

Quantitative microinjection of oocytes, eggs, and embryos. Methods Cell Biol (2004) 1.21

Construction and characterization of new piggyBac vectors for constitutive or inducible expression of heterologous gene pairs and the identification of a previously unrecognized activator sequence in piggyBac. BMC Biotechnol (2007) 1.14

Retrovirus silencing and vector design: relevance to normal and cancer stem cells? Curr Gene Ther (2005) 1.12

Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis. Proc Natl Acad Sci U S A (2012) 1.10

Predicting preferential DNA vector insertion sites: implications for functional genomics and gene therapy. Genome Biol (2007) 1.08

Evaluating risks of insertional mutagenesis by DNA transposons in gene therapy. Transl Res (2013) 1.01

Theoretical mechanisms in targeted and random integration of transgene DNA. Reprod Nutr Dev (2002) 0.96

Paternal DNA damage resulting from various sperm treatments persists after fertilization and is similar before and after DNA replication. J Androl (2011) 0.91

Cis-acting gene regulatory activities in the terminal regions of sleeping beauty DNA transposon-based vectors. Hum Gene Ther (2007) 0.89

Expression of foreign DNA is associated with paternal chromosome degradation in intracytoplasmic sperm injection-mediated transgenesis in the mouse. Biol Reprod (2002) 0.89

Efficient non-viral integration and stable gene expression in multipotent adult progenitor cells. Stem Cells Int (2011) 0.78

Articles by these authors

piggyBac is a flexible and highly active transposon as compared to sleeping beauty, Tol2, and Mos1 in mammalian cells. Proc Natl Acad Sci U S A (2006) 3.35

Helper-independent piggyBac plasmids for gene delivery approaches: strategies for avoiding potential genotoxic effects. Proc Natl Acad Sci U S A (2010) 1.25

Hyperactive self-inactivating piggyBac for transposase-enhanced pronuclear microinjection transgenesis. Proc Natl Acad Sci U S A (2012) 1.10

Chimeric piggyBac transposases for genomic targeting in human cells. Nucleic Acids Res (2012) 1.09

Transcription activator like effector (TALE)-directed piggyBac transposition in human cells. Nucleic Acids Res (2013) 1.01

Transgenic over-expression of growth differentiation factor 11 propeptide in skeleton results in transformation of the seventh cervical vertebra into a thoracic vertebra. Mol Reprod Dev (2010) 0.94

Regulation and effects of modulation of telomerase reverse transcriptase expression in primordial germ cells during development. Biol Reprod (2006) 0.92

Active integration: new strategies for transgenesis. Transgenic Res (2007) 0.91

Expression of foreign DNA is associated with paternal chromosome degradation in intracytoplasmic sperm injection-mediated transgenesis in the mouse. Biol Reprod (2002) 0.89

Tn5 transposase-mediated mouse transgenesis. Biol Reprod (2005) 0.88

Generation of transgenic pigs by cytoplasmic injection of piggyBac transposase-based pmGENIE-3 plasmids. Biol Reprod (2014) 0.86

Genetic vasectomy-overexpression of Prm1-EGFP fusion protein in elongating spermatids causes dominant male sterility in mice. Genesis (2010) 0.85

Pig transgenesis by piggyBac transposition in combination with somatic cell nuclear transfer. Transgenic Res (2013) 0.84

Adjuvants may reduce in vivo transfection levels for DNA vaccination in mice leading to reduced antigen-specific CD8+ T cell responses. Hum Vaccin Immunother (2015) 0.83

PhiC31/PiggyBac modified stromal stem cells: effect of interferon γ and/or tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) on murine melanoma. Mol Cancer (2014) 0.83

Production of inbred and hybrid transgenic mice carrying large (> 200 kb) foreign DNA fragments by intracytoplasmic sperm injection. Mol Reprod Dev (2005) 0.83

Lactate and adenosine triphosphate in the extender enhance the cryosurvival of rat epididymal sperm. J Am Assoc Lab Anim Sci (2010) 0.81

Extracellular ATP and dibutyryl cAMP enhance the freezability of rat epididymal sperm. J Am Assoc Lab Anim Sci (2010) 0.78

Effective Targeted Gene Knockdown in Mammalian Cells Using the piggyBac Transposase-based Delivery System. Mol Ther Nucleic Acids (2013) 0.77

Recombinase-mediated mouse transgenesis by intracytoplasmic sperm injection. Theriogenology (2005) 0.75