Correlative cryo-electron tomography and optical microscopy of cells.

PubWeight™: 0.98‹?› | Rank: Top 15%

🔗 View Article (PMC 3812453)

Published in Curr Opin Struct Biol on August 17, 2013

Authors

Peijun Zhang1

Author Affiliations

1: Department of Structural Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15260, USA. Electronic address: pez7@pitt.edu.

Articles cited by this

Imaging intracellular fluorescent proteins at nanometer resolution. Science (2006) 38.97

Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM). Nat Methods (2006) 30.70

Nonlinear structured-illumination microscopy: wide-field fluorescence imaging with theoretically unlimited resolution. Proc Natl Acad Sci U S A (2005) 12.21

Toward fluorescence nanoscopy. Nat Biotechnol (2003) 8.63

Molecular architecture of native HIV-1 gp120 trimers. Nature (2008) 8.04

Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. Science (2008) 6.47

Multicolor and electron microscopic imaging of connexin trafficking. Science (2002) 5.60

Super-resolution fluorescence microscopy. Annu Rev Biochem (2009) 5.47

A guide to super-resolution fluorescence microscopy. J Cell Biol (2010) 4.71

Software for bead-based registration of selective plane illumination microscopy data. Nat Methods (2010) 4.71

Macromolecular crowding: an important but neglected aspect of the intracellular environment. Curr Opin Struct Biol (2001) 4.27

Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B. subtilis. Science (2011) 4.26

A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. PLoS Biol (2011) 4.07

Nuclear pore complex structure and dynamics revealed by cryoelectron tomography. Science (2004) 3.61

Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria. Science (2011) 3.47

Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. EMBO J (2007) 3.17

Snapshots of nuclear pore complexes in action captured by cryo-electron tomography. Nature (2007) 2.99

The bacterial actin MreB rotates, and rotation depends on cell-wall assembly. Proc Natl Acad Sci U S A (2011) 2.61

Structure and assembly of immature HIV. Proc Natl Acad Sci U S A (2009) 2.51

Protein localization in electron micrographs using fluorescence nanoscopy. Nat Methods (2010) 2.33

Correlative microscopy: bridging the gap between fluorescence light microscopy and cryo-electron tomography. J Struct Biol (2007) 2.29

Cryo-electron microscopy of vitreous sections. EMBO J (2004) 2.03

Focused-ion-beam thinning of frozen-hydrated biological specimens for cryo-electron microscopy. Nat Methods (2007) 1.92

Visualizing cells at the nanoscale. Trends Biochem Sci (2008) 1.91

Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography. Proc Natl Acad Sci U S A (2012) 1.89

Dissecting the molecular architecture of integrin adhesion sites by cryo-electron tomography. Nat Cell Biol (2010) 1.72

Toward detecting and identifying macromolecules in a cellular context: template matching applied to electron tomograms. Proc Natl Acad Sci U S A (2000) 1.67

Cryo-fluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching. J Microsc (2007) 1.59

The helical MreB cytoskeleton in Escherichia coli MC1000/pLE7 is an artifact of the N-Terminal yellow fluorescent protein tag. J Bacteriol (2012) 1.58

Direct visualization of receptor arrays in frozen-hydrated sections and plunge-frozen specimens of E. coli engineered to overproduce the chemotaxis receptor Tsr. J Microsc (2004) 1.56

Cryo-electron tomography of bacteria: progress, challenges and future prospects. Nat Rev Microbiol (2009) 1.51

Micromachining tools and correlative approaches for cellular cryo-electron tomography. J Struct Biol (2010) 1.49

Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections. Nat Methods (2008) 1.40

Integrated fluorescence and transmission electron microscopy. J Struct Biol (2008) 1.40

Cryo electron tomography of native HIV-1 budding sites. PLoS Pathog (2010) 1.39

Long helical filaments are not seen encircling cells in electron cryotomograms of rod-shaped bacteria. Biochem Biophys Res Commun (2011) 1.35

Tools for correlative cryo-fluorescence microscopy and cryo-electron tomography applied to whole mitochondria in human endothelial cells. Eur J Cell Biol (2009) 1.33

Correlative 3D imaging of whole mammalian cells with light and electron microscopy. J Struct Biol (2011) 1.33

Correlative cryo-light microscopy and cryo-electron tomography: from cellular territories to molecular landscapes. Curr Opin Biotechnol (2009) 1.32

Multiscale imaging of neurons grown in culture: from light microscopy to cryo-electron tomography. J Struct Biol (2007) 1.32

Electron cryotomography: a new view into microbial ultrastructure. Curr Opin Microbiol (2009) 1.26

The three-dimensional organization of polyribosomes in intact human cells. Mol Cell (2010) 1.26

Concatenated metallothionein as a clonable gold label for electron microscopy. J Struct Biol (2007) 1.20

Direct visualization of HIV-1 with correlative live-cell microscopy and cryo-electron tomography. Structure (2011) 1.14

Visualizing cellular processes at the molecular level by cryo-electron tomography. J Cell Sci (2010) 1.12

Dynamics of podosome stiffness revealed by atomic force microscopy. Proc Natl Acad Sci U S A (2010) 1.10

Live cell imaging of the HIV-1 life cycle. Trends Microbiol (2008) 1.08

Correlative VIS-fluorescence and soft X-ray cryo-microscopy/tomography of adherent cells. J Struct Biol (2011) 1.06

Electron tomography of vitreous sections from cultured mammalian cells. J Struct Biol (2007) 1.06

Molecular structures of trimeric HIV-1 Env in complex with small antibody derivatives. Proc Natl Acad Sci U S A (2012) 1.06

Cryo electron tomography of herpes simplex virus during axonal transport and secondary envelopment in primary neurons. PLoS Pathog (2011) 1.05

From dynamic live cell imaging to 3D ultrastructure: novel integrated methods for high pressure freezing and correlative light-electron microscopy. PLoS One (2010) 1.02

3D structure determination of native mammalian cells using cryo-FIB and cryo-electron tomography. J Struct Biol (2012) 1.01

The actin-like MreB proteins in Bacillus subtilis: a new turn. Front Biosci (Schol Ed) (2012) 0.97

Microtubule organization in the final stages of cytokinesis as revealed by cryo-electron tomography. J Cell Sci (2011) 0.95

Fully hydrated yeast cells imaged with electron microscopy. Biophys J (2011) 0.93

Localization of fluorescently labeled structures in frozen-hydrated samples using integrated light electron microscopy. J Struct Biol (2012) 0.88

Correlative microscopy methods that maximize specimen fidelity and data completeness, and improve molecular localization capabilities. J Struct Biol (2013) 0.85

Rotate into shape: MreB and bacterial morphogenesis. EMBO J (2011) 0.80

Correlative microscopy for 3D structural analysis of dynamic interactions. J Vis Exp (2013) 0.80

Low-cost cryo-light microscopy stage fabrication for correlated light/electron microscopy. J Vis Exp (2011) 0.79