Modulation of the microenvironment surrounding the active site of penicillin G acylase immobilized on acrylic carriers improves the enzymatic synthesis of cephalosporins.

Uricase alkaline enzymosomes with enhanced stabilities and anti-hyperuricemia effects induced by favorable microenvironmental changes. Sci Rep (2016) 0.75

New active site oriented glyoxyl-agarose derivatives of Escherichia coli penicillin G acylase. BMC Biotechnol (2007) 0.90

Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins. Microb Cell Fact (2013) 0.83

Immobilization and stabilization of recombinant multimeric uridine and purine nucleoside phosphorylases from Bacillus subtilis. Biomacromolecules (2004) 0.78

Improvement of catalytic properties of Escherichia coli penicillin G acylase immobilized on glyoxyl agarose by addition of a six-amino-acid tag. Appl Environ Microbiol (2005) 0.77

Enzymatic reduction of acetophenone derivatives with a benzil reductase from Pichia glucozyma (KRED1-Pglu): electronic and steric effects on activity and enantioselectivity. Org Biomol Chem (2016) 0.76

Regioselective monodeprotection of peracetylated carbohydrates. Nat Protoc (2012) 0.76

Stabilization of thymidine phosphorylase from Escherichia coli by immobilization and post immobilization techniques. Enzyme Microb Technol (2011) 0.75

Characterization and study of the orientation of immobilized enzymes by tryptic digestion and HPLC-MS: design of an efficient catalyst for the synthesis of cephalosporins. Biomacromolecules (2010) 0.75

Immobilization of the acylase from Escherichia coli on glyoxyl-agarose gives efficient catalyst for the synthesis of cephalosporins. Enzyme Microb Technol (2007) 0.75

New N-alkyl-1,2-dihydro-2-thioxo-3-pyridinecarbothioamides as antituberculous agents with improved pharmacokinetics. Bioorg Med Chem Lett (2002) 0.75

Enzymatic synthesis of cephalosporins. The immobilized acylase from Arthrobacter viscosus: a new useful biocatalyst. Appl Microbiol Biotechnol (2007) 0.75

1,2-dithiolan-3-ones and derivatives structurally related to leinamycin. Synthesis and biological evaluation. Farmaco (2003) 0.75

Efficient biocatalyst for large-scale synthesis of cephalosporins, obtained by combining immobilization and site-directed mutagenesis of penicillin acylase. Appl Microbiol Biotechnol (2012) 0.75

Assessment of immobilized PGA orientation via the LC-MS analysis of tryptic digests of the wild type and its 3K-PGA mutant assists in the rational design of a high-performance biocatalyst. Anal Bioanal Chem (2012) 0.75