Ecto-Fc MS identifies ligand-receptor interactions through extracellular domain Fc fusion protein baits and shotgun proteomic analysis.

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Published in Nat Protoc on August 07, 2014

Authors

Jeffrey N Savas1, Joris De Wit2, Davide Comoletti3, Roland Zemla4, Anirvan Ghosh5, John R Yates6

Author Affiliations

1: 1] Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA. [2].
2: 1] Vlaams Instituut voor Biotechnologie (VIB) Center for the Biology of Disease, Leuven, Belgium. [2] Center for Human Genetics, Katholieke Universiteit (KU) Leuven, Leuven, Belgium. [3] Neurobiology Section, Division of Biology, University of California San Diego, La Jolla, California, USA. [4].
3: Child Health Institute of New Jersey and Department of Neuroscience and Cell Biology, Robert Wood Johnson Medical School, Rutgers University, New Brunswick, New Jersey, USA.
4: New York University School of Medicine, New York, New York, USA.
5: 1] Neurobiology Section, Division of Biology, University of California San Diego, La Jolla, California, USA. [2] Neuroscience Discovery, F. Hoffman-La Roche, Basel, Switzerland.
6: Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California, USA.

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