Cationic Nanogel-mediated Runx2 and Osterix siRNA Delivery Decreases Mineralization in MC3T3 Cells.

PubWeight™: 0.80‹?›

🔗 View Article (PMID 25448327)

Published in Clin Orthop Relat Res on December 02, 2014

Authors

Arun R Shrivats1, Eric Hsu, Saadyah Averick, Molly Klimak, April C S Watt, Marlene DeMaio, Krzysztof Matyjaszewski, Jeffrey O Hollinger

Author Affiliations

1: Department of Biomedical Engineering, Carnegie Mellon University, 700 Technology Drive, Pittsburgh, PA, 15219, USA.

Articles cited by this

Unlocking the potential of the human genome with RNA interference. Nature (2004) 6.70

Pharmacological and biochemical demonstration of the role of cyclooxygenase 2 in inflammation and pain. Proc Natl Acad Sci U S A (1994) 4.21

Heterotopic ossification. J Am Acad Orthop Surg (2004) 2.96

Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. J Bone Miner Res (1999) 2.94

Differential temporal expression of members of the transforming growth factor beta superfamily during murine fracture healing. J Bone Miner Res (2002) 2.62

Cyclooxygenase-2 (COX-2) in inflammatory and degenerative brain diseases. J Neuropathol Exp Neurol (2004) 2.45

Runx2-mediated regulation of the zinc finger Osterix/Sp7 gene. Gene (2006) 2.16

BMP2 commitment to the osteogenic lineage involves activation of Runx2 by DLX3 and a homeodomain transcriptional network. J Biol Chem (2006) 2.15

Heterotopic ossification. J Nucl Med (2002) 2.13

The silent treatment: siRNAs as small molecule drugs. Gene Ther (2006) 2.12

Dlx3 transcriptional regulation of osteoblast differentiation: temporal recruitment of Msx2, Dlx3, and Dlx5 homeodomain proteins to chromatin of the osteocalcin gene. Mol Cell Biol (2004) 1.99

VEGF improves, whereas sFlt1 inhibits, BMP2-induced bone formation and bone healing through modulation of angiogenesis. J Bone Miner Res (2005) 1.96

BMP-2 and insulin-like growth factor-I mediate Osterix (Osx) expression in human mesenchymal stem cells via the MAPK and protein kinase D signaling pathways. J Biol Chem (2005) 1.92

Critical molecular switches involved in BMP-2-induced osteogenic differentiation of mesenchymal cells. Gene (2005) 1.91

Structural coupling of Smad and Runx2 for execution of the BMP2 osteogenic signal. J Biol Chem (2008) 1.84

In vitro osteogenesis assays: influence of the primary cell source on alkaline phosphatase activity and mineralization. Pathol Biol (Paris) (2008) 1.77

Observations on the induction of bone in soft tissues. J Bone Joint Surg Br (1975) 1.68

BMP-2 induces Osterix expression through up-regulation of Dlx5 and its phosphorylation by p38. J Biol Chem (2007) 1.65

Heterotopic ossification following traumatic and combat-related amputations. Prevalence, risk factors, and preliminary results of excision. J Bone Joint Surg Am (2007) 1.58

Oxygen radical-mediated pulmonary toxicity induced by some cationic liposomes. Pharm Res (2000) 1.57

Bone morphogenetic protein 2 (BMP-2) enhances BMP-3, BMP-4, and bone cell differentiation marker gene expression during the induction of mineralized bone matrix formation in cultures of fetal rat calvarial osteoblasts. Calcif Tissue Int (1997) 1.51

Osx transcriptional regulation is mediated by additional pathways to BMP2/Smad signaling. J Cell Biochem (2005) 1.48

BMP treatment of C3H10T1/2 mesenchymal stem cells induces both chondrogenesis and osteogenesis. J Cell Biochem (2003) 1.48

Examination of mineralized nodule formation in living osteoblastic cultures using fluorescent dyes. Biotechnol Prog (2006) 1.43

Biodegradable nanogels prepared by atom transfer radical polymerization as potential drug delivery carriers: synthesis, biodegradation, in vitro release, and bioconjugation. J Am Chem Soc (2007) 1.38

Bone morphogenetic proteins: an update on basic biology and clinical relevance. J Orthop Res (1999) 1.34

Transcriptional regulation of bone formation by the osteoblast-specific transcription factor Osx. J Orthop Surg Res (2010) 1.27

Engineered bone development from a pre-osteoblast cell line on three-dimensional scaffolds. Tissue Eng (2000) 1.23

Periarticular heterotopic ossification after total hip arthroplasty. Risk factors and consequences. Clin Orthop Relat Res (1991) 1.20

Regulation of bone morphogenetic protein-2 expression by endogenous prostaglandin E2 in human mesenchymal stem cells. J Cell Physiol (2004) 1.19

Heterotopic ossification in wartime wounds. J Surg Orthop Adv (2010) 1.16

Heterotopic ossification of the elbow in patients with burns. Results after early excision. J Bone Joint Surg Br (2004) 1.15

Expression and regulation of Runx2/Cbfa1 and osteoblast phenotypic markers during the growth and differentiation of human osteoblasts. J Cell Biochem (2001) 1.10

Runx2 integrates estrogen activity in osteoblasts. J Biol Chem (2003) 1.10

Prevention of heterotopic ossification after spinal cord injury with indomethacin. Spinal Cord (2001) 1.09

Runx2/Cbfa1 stimulates transdifferentiation of primary skeletal myoblasts into a mineralizing osteoblastic phenotype. Exp Cell Res (2004) 1.06

Inverse miniemulsion ATRP: a new method for synthesis and functionalization of well-defined water-soluble/cross-linked polymeric particles. J Am Chem Soc (2006) 1.03

Macromolecular engineering by atom transfer radical polymerization. J Am Chem Soc (2014) 1.02

Adenovirus-mediated transfer of siRNA against Runx2/Cbfa1 inhibits the formation of heterotopic ossification in animal model. Biochem Biophys Res Commun (2006) 1.01

Animal models of typical heterotopic ossification. J Biomed Biotechnol (2010) 1.00

Blast injuries and heterotopic ossification. Bone Joint Res (2012) 0.99

Osteopontin is a negative regulator of proliferation and differentiation in MC3T3-E1 pre-osteoblastic cells. Bone (2004) 0.97

Early excision and late excision of heterotopic ossification after traumatic brain injury are equivalent: a systematic review of the literature. J Neurotrauma (2007) 0.97

Heterotopic ossification in the residual limbs of traumatic and combat-related amputees. J Am Acad Orthop Surg (2006) 0.96

A comparison of heterotopic ossification treatment within the traumatic brain and spinal cord injured population: An evidence based systematic review. NeuroRehabilitation (2011) 0.94

Osteosarcoma following single fraction radiation prophylaxis for heterotopic ossification. Radiat Oncol (2012) 0.93

Synthesis and biodegradation of nanogels as delivery carriers for carbohydrate drugs. Biomacromolecules (2007) 0.91

Heterotopic ossification and peripheral nerve entrapment: early diagnosis and excision. Arch Phys Med Rehabil (1991) 0.91

Runt domain factor (Runx)-dependent effects on CCAAT/ enhancer-binding protein delta expression and activity in osteoblasts. J Biol Chem (2000) 0.90

Radiation prophylaxis for heterotopic ossification about the hip joint--a multicenter study. Int J Radiat Oncol Biol Phys (2001) 0.89

Heterotopic ossification following burn injury: the role of stem cells. J Burn Care Res (2012) 0.89

Prevention of heterotopic ossification after spinal cord injury with COX-2 selective inhibitor (rofecoxib). Spinal Cord (2004) 0.89

Heterotopic ossification following traumatic brain injury and spinal cord injury: insight into the etiology and pathophysiology. J Musculoskelet Neuronal Interact (2012) 0.89

Delivery of siRNA silencing Runx2 using a multifunctional polymer-lipid nanoparticle inhibits osteogenesis in a cell culture model of heterotopic ossification. Integr Biol (Camb) (2012) 0.88

The sequential production profiles of growth factors and their relations to bone volume in ossifying bone marrow explants. Tissue Eng Part A (2010) 0.85

Heterotopic ossification after 2-incision total hip arthroplasty. J Arthroplasty (2009) 0.83

Characterization of heterotopic ossification in burn patients. J Burn Care Res (2014) 0.82

PGE₂ and BMP-2 in bone and cartilage metabolism: 2 intertwining pathways. Can J Physiol Pharmacol (2012) 0.81

Cationic nanostructured polymers for siRNA delivery in murine calvarial pre-osteoblasts. J Biomed Nanotechnol (2014) 0.81

Risk factors for developing heterotopic ossification in patients with traumatic brain injury. Brain Inj (2013) 0.79

Preparation of cationic nanogels for nucleic acid delivery. Biomacromolecules (2012) 0.79

Calcification of primary human osteoblast cultures under flow conditions using polycaprolactone scaffolds for intravascular applications. J Tissue Eng Regen Med (2011) 0.77

Heterotopic ossification in combat amputees from Afghanistan and Iraq wars: five case histories and results from a small series of patients. J Rehabil Res Dev (2011) 0.76

Articles by these authors

Adsorption-induced scission of carbon-carbon bonds. Nature (2006) 2.20

Design and preparation of porous polymers. Chem Rev (2012) 1.64

Permanent, non-leaching antibacterial surface--2: how high density cationic surfaces kill bacterial cells. Biomaterials (2007) 1.48

Osx transcriptional regulation is mediated by additional pathways to BMP2/Smad signaling. J Cell Biochem (2005) 1.48

The design and use of animal models for translational research in bone tissue engineering and regenerative medicine. Tissue Eng Part B Rev (2010) 1.47

Biodegradable nanogels prepared by atom transfer radical polymerization as potential drug delivery carriers: synthesis, biodegradation, in vitro release, and bioconjugation. J Am Chem Soc (2007) 1.38

Synthesis of molecular brushes by "grafting onto" method: combination of ATRP and click reactions. J Am Chem Soc (2007) 1.36

Fracture healing in the elderly patient. Exp Gerontol (2006) 1.32

Synthesis of uniform protein-polymer conjugates. Biomacromolecules (2005) 1.30

Context-dependent role of angiopoietin-1 inhibition in the suppression of angiogenesis and tumor growth: implications for AMG 386, an angiopoietin-1/2-neutralizing peptibody. Mol Cancer Ther (2010) 1.27

Application of histomorphometric methods to the study of bone repair. J Bone Miner Res (2005) 1.26

Demineralized bone matrix in bone repair: history and use. Adv Drug Deliv Rev (2012) 1.22

Synthesis and in vitro biocompatibility of injectable polyurethane foam scaffolds. Tissue Eng (2006) 1.22

Atom transfer radical addition and polymerization reactions catalyzed by ppm amounts of copper complexes. Chem Soc Rev (2008) 1.21

Light-induced reversible formation of polymeric micelles. Angew Chem Int Ed Engl (2007) 1.19

ATRP in the design of functional materials for biomedical applications. Prog Polym Sci (2011) 1.15

Ionic strength and composition affect the mobility of surface-modified Fe0 nanoparticles in water-saturated sand columns. Environ Sci Technol (2008) 1.14

Influence of the degree of methacrylation on hyaluronic acid hydrogels properties. Biomaterials (2008) 1.12

Permanent, nonleaching antibacterial surfaces. 1. Synthesis by atom transfer radical polymerization. Biomacromolecules (2004) 1.10

Marrying click chemistry with polymerization: expanding the scope of polymeric materials. Chem Soc Rev (2009) 1.10

One-pot synthesis of robust core/shell gold nanoparticles. J Am Chem Soc (2008) 1.09

"Green" atom transfer radical polymerization: from process design to preparation of well-defined environmentally friendly polymeric materials. Chem Rev (2007) 1.08

Real-time intersession and interrater reliability of the functional movement screen. J Strength Cond Res (2012) 1.07

Synthesis of biocompatible segmented polyurethanes from aliphatic diisocyanates and diurea diol chain extenders. Acta Biomater (2005) 1.05

Synthesis, mechanical properties, biocompatibility, and biodegradation of polyurethane networks from lysine polyisocyanates. Biomaterials (2008) 1.03

Activators regenerated by electron transfer for atom-transfer radical polymerization of (meth)acrylates and related block copolymers. Angew Chem Int Ed Engl (2006) 1.03

Inverse miniemulsion ATRP: a new method for synthesis and functionalization of well-defined water-soluble/cross-linked polymeric particles. J Am Chem Soc (2006) 1.03

Low polydispersity star polymers via cross-linking macromonomers by ATRP. J Am Chem Soc (2006) 1.01

Flexible particle array structures by controlling polymer graft architecture. J Am Chem Soc (2010) 1.00

Osteoblast differentiation in vitro and in vivo promoted by Osterix. J Biomed Mater Res A (2007) 1.00

Nanostructured hybrid hydrogels prepared by a combination of atom transfer radical polymerization and free radical polymerization. Biomaterials (2009) 0.98

Understanding atom transfer radical polymerization: effect of ligand and initiator structures on the equilibrium constants. J Am Chem Soc (2008) 0.98

Highly active copper-based catalyst for atom transfer radical polymerization. J Am Chem Soc (2006) 0.98

Porous polymer scaffolds surface-modified with arginine-glycine-aspartic acid enhance bone cell attachment and differentiation in vitro. J Biomed Mater Res A (2003) 0.96

Genetically encoded initiator for polymer growth from proteins. J Am Chem Soc (2010) 0.96

Synthesis, characterization, and in vitro cell culture viability of degradable poly(N-isopropylacrylamide-co-5,6-benzo-2-methylene-1,3-dioxepane)-based polymers and crosslinked gels. J Biomed Mater Res A (2008) 0.96

Repeatable photoinduced self-healing of covalently cross-linked polymers through reshuffling of trithiocarbonate units. Angew Chem Int Ed Engl (2011) 0.95

Nonleaching antibacterial glass surfaces via "Grafting Onto": the effect of the number of quaternary ammonium groups on biocidal activity. Langmuir (2008) 0.93

Augment bone graft products compare favorably with autologous bone graft in an ovine model of lumbar interbody spine fusion. Spine (Phila Pa 1976) (2012) 0.93

Commercially available demineralized bone matrix compositions to regenerate calvarial critical-sized bone defects. Plast Reconstr Surg (2006) 0.93

On the shape of bottle-brush macromolecules: systematic variation of architectural parameters. J Chem Phys (2005) 0.93

Preparation of homopolymers and block copolymers in miniemulsion by ATRP using activators generated by electron transfer (AGET). J Am Chem Soc (2005) 0.92

Fabrication of poly(alpha-hydroxy acid) foam scaffolds using multiple solvent systems. J Biomed Mater Res (2002) 0.92

Synthesis, biodegradability, and biocompatibility of lysine diisocyanate-glucose polymers. Tissue Eng (2002) 0.92

rhBMP-2 delivered in a calcium phosphate cement accelerates bridging of critical-sized defects in rabbit radii. J Bone Joint Surg Am (2006) 0.92

Synthesis and characterization of segmented poly(esterurethane urea) elastomers for bone tissue engineering. Acta Biomater (2007) 0.92

Electrochemically mediated atom transfer radical polymerization. Science (2011) 0.91

Synthesis and biodegradation of nanogels as delivery carriers for carbohydrate drugs. Biomacromolecules (2007) 0.91

Thermoresponsive hydrogel scaffolds with tailored hydrophilic pores. Chem Asian J (2011) 0.91

Bone regeneration in a rabbit critical-sized calvarial model using tyrosine-derived polycarbonate scaffolds. Tissue Eng Part A (2012) 0.90

Photo-cross-linkable thermoresponsive star polymers designed for control of cell-surface interactions. Biomacromolecules (2010) 0.90

Osteogenic differentiation of pre-osteoblasts on biomimetic tyrosine-derived polycarbonate scaffolds. Biomacromolecules (2011) 0.90

BaBa-xy16: robust and broadband homonuclear DQ recoupling for applications in rigid and soft solids up to the highest MAS frequencies. J Magn Reson (2011) 0.90

Composition options for tissue-engineered bone. Tissue Eng (2002) 0.90

Determination of equilibrium constants for atom transfer radical polymerization. J Am Chem Soc (2006) 0.90

Perfect mixing of immiscible macromolecules at fluid interfaces. Nat Mater (2013) 0.90

A silver bullet: elemental silver as an efficient reducing agent for atom transfer radical polymerization of acrylates. J Am Chem Soc (2015) 0.89

A biodegradable polyurethane-ascorbic acid scaffold for bone tissue engineering. J Biomed Mater Res A (2003) 0.89

Templated synthesis of nitrogen-enriched nanoporous carbon materials from porogenic organic precursors prepared by ATRP. Angew Chem Int Ed Engl (2014) 0.89

Synthesis of well-defined microporous carbons by molecular-scale templating with polyhedral oligomeric silsesquioxane moieties. J Am Chem Soc (2014) 0.89

Synthesis of monofunctional curcumin derivatives, clicked curcumin dimer, and a PAMAM dendrimer curcumin conjugate for therapeutic applications. Org Lett (2007) 0.89

Rapid-prototyped PLGA/β-TCP/hydroxyapatite nanocomposite scaffolds in a rabbit femoral defect model. Biofabrication (2012) 0.88

Well-defined carbon nanoparticles prepared from water-soluble shell cross-linked micelles that contain polyacrylonitrile cores. Angew Chem Int Ed Engl (2004) 0.88

Antibacterial polypropylene via surface-initiated atom transfer radical polymerization. Biomacromolecules (2007) 0.88

Autotransfecting short interfering RNA through facile covalent polymer escorts. J Am Chem Soc (2013) 0.86

Long-range ordered thin films of block copolymers prepared by zone-casting and their thermal conversion into ordered nanostructured carbon. J Am Chem Soc (2005) 0.86

Serotonin reuptake inhibitors and bleeding risks in major orthopedic procedures. Psychosomatics (2012) 0.86

Three-dimensional biocompatible ascorbic acid-containing scaffold for bone tissue engineering. Tissue Eng (2003) 0.86

Synthesis by AGET ATRP of degradable nanogel precursors for in situ formation of nanostructured hyaluronic acid hydrogel. Biomacromolecules (2009) 0.85

Gene expression profiling of bone marrow stromal cells from juvenile, adult, aged and osteoporotic rats: with an emphasis on osteoporosis. Bone (2006) 0.85

Cellular uptake of functional nanogels prepared by inverse miniemulsion ATRP with encapsulated proteins, carbohydrates, and gold nanoparticles. Biomacromolecules (2009) 0.85

Bone tissue engineering: recent advances and promising therapeutic agents. Expert Opin Biol Ther (2003) 0.85

Electrochemically active nitrogen-enriched nanocarbons with well-defined morphology synthesized by pyrolysis of self-assembled block copolymer. J Am Chem Soc (2012) 0.84

Preparation of polymeric nanoscale networks from cylindrical molecular bottlebrushes. ACS Nano (2012) 0.84

Direct DNA conjugation to star polymers for controlled reversible assemblies. Bioconjug Chem (2011) 0.84

A protein-polymer hybrid mediated by DNA. Langmuir (2012) 0.84

End-group effects on the properties of PEG-co-PGA hydrogels. Acta Biomater (2009) 0.84

A calcium phosphate-based gene delivery system. J Biomed Mater Res A (2005) 0.84

Self-healing of covalently cross-linked polymers by reshuffling thiuram disulfide moieties in air under visible light. Adv Mater (2012) 0.83

Measuring molecular weight by atomic force microscopy. J Am Chem Soc (2003) 0.83

Influence of cross-linker chemistry on release kinetics of PEG-co-PGA hydrogels. J Biomed Mater Res A (2009) 0.83

Regenerative tendon and ligament healing: opportunities with recombinant human platelet-derived growth factor BB-homodimer. Tissue Eng Part B Rev (2012) 0.83

Properties and ATRP activity of copper complexes with substituted tris(2-pyridylmethyl)amine-based ligands. Inorg Chem (2015) 0.83

Using mesoscopic models to design strong and tough biomimetic polymer networks. Langmuir (2011) 0.82

Harnessing labile bonds between nanogel particles to create self-healing materials. ACS Nano (2009) 0.82

Dual-reactive surfactant used for synthesis of functional nanocapsules in miniemulsion. J Am Chem Soc (2010) 0.82

Biologically derived soft conducting hydrogels using heparin-doped polymer networks. ACS Nano (2014) 0.82

Cell-adhesive star polymers prepared by ATRP. Biomacromolecules (2009) 0.82

Adsorbed triblock copolymers deliver reactive iron nanoparticles to the oil/water interface. Nano Lett (2005) 0.82

A scanning force microscopy study on the motion of single brush-like macromolecules on a silicon substrate induced by coadsorption of small molecules. Phys Chem Chem Phys (2006) 0.82