Published in Mol Cells on May 03, 2016
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene (1988) 52.71
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol (1985) 25.52
Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. Mol Cell Biol (1988) 13.78
Epitope tagging and protein surveillance. Methods Enzymol (1991) 6.47
Metal chelate affinity chromatography, a new approach to protein fractionation. Nature (1975) 5.83
Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol (2002) 4.22
Prophylactic and therapeutic efficacy of human monoclonal antibodies against H5N1 influenza. PLoS Med (2007) 3.63
Making the most of affinity tags. Trends Biotechnol (2005) 2.48
Bacteriophytochromes are photochromic histidine kinases using a biliverdin chromophore. Nature (2001) 2.22
Bacteriophytochromes: phytochrome-like photoreceptors from nonphotosynthetic eubacteria. Science (1999) 2.10
Epitope tagging. Annu Rev Genet (1998) 1.83
Mapping Epitope Structure and Activity: From One-Dimensional Prediction to Four-Dimensional Description of Antigenic Specificity Methods (1996) 1.63
The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng (1993) 1.54
Engineering proteins to facilitate bioprocessing. Trends Biotechnol (1994) 1.43
His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors. J Struct Funct Genomics (2004) 1.31
Attachment of a histidine tag to the minimal zinc finger protein of the Aspergillus nidulans gene regulatory protein AreA causes a conformational change at the DNA-binding site. Protein Expr Purif (2005) 1.30
Production in Escherichia coli and one-step purification of bifunctional hybrid proteins which bind maltose. Export of the Klenow polymerase into the periplasmic space. Eur J Biochem (1988) 1.29
Hexahistidine (His6)-tag dependent protein dimerization: a cautionary tale. Acta Biochim Pol (1999) 1.28
Differential effects of short affinity tags on the crystallization of Pyrococcus furiosus maltodextrin-binding protein. Acta Crystallogr D Biol Crystallogr (2002) 1.25
Generation and application of new rat monoclonal antibodies against synthetic FLAG and OLLAS tags for improved immunodetection. J Immunol Methods (2007) 1.05
Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins. J Struct Funct Genomics (2002) 1.03
Epitope tagging. Biotechniques (2008) 1.03
HER2 therapies and gastric cancer: a step forward. World J Gastroenterol (2013) 0.96
Properties of soluble fusions between mammalian aspartic proteinases and bacterial maltose-binding protein. J Protein Chem (1999) 0.95
Idiotypes as immunogens: facing the challenge of inducing strong therapeutic immune responses against the variable region of immunoglobulins. Front Oncol (2012) 0.92
Relative position of the hexahistidine tag effects binding properties of a tumor-associated single-chain Fv construct. Biochim Biophys Acta (2000) 0.90
Respiratory syncytial virus disease: prevention and treatment. Curr Top Microbiol Immunol (2013) 0.87
Epitope-tagged gonadotropin-releasing hormone receptors heterologously-expressed in mammalian (COS-1) and insect (Sf9) cells. Mol Cell Endocrinol (1997) 0.82
Monoclonal antibodies in pediatrics: use in prevention and treatment. Allergol Immunopathol (Madr) (2007) 0.81
A novel anti-human syndecan-1 (CD138) monoclonal antibody 4B3: characterization and application. Cell Mol Immunol (2007) 0.80
An efficient tag derived from the common epitope of tospoviral NSs proteins for monitoring recombinant proteins expressed in both bacterial and plant systems. J Biotechnol (2013) 0.79
Fast and efficient characterization of an anti-gliadin monoclonal antibody epitope related to celiac disease using resin-bound peptides. J Immunol Methods (2010) 0.79
Chemical synthesis and cloning of a poly(arginine)-coding gene fragment designed to aid polypeptide purification. Gene (1984) 0.79
Epitope mapping of monoclonal antibodies for the Deinococcus radiodurans bacteriophytochome. Protein Sci (2014) 0.77