Rational design of reversible fluorescent probes for live-cell imaging and quantification of fast glutathione dynamics.

PubWeight™: 0.78‹?›

🔗 View Article (PMID 28221345)

Published in Nat Chem on November 07, 2016

Authors

Keitaro Umezawa1,2, Masafumi Yoshida1,3, Mako Kamiya1,4, Tatsuya Yamasoba3, Yasuteru Urano1,2,5

Author Affiliations

1: Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 0033, Japan.
2: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 0033, Japan.
3: Department of Otolaryngology - Head and Neck Surgery, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 0033, Japan.
4: PRESTO, Japan Science and Technology (JST) Agency, Honcho, Kawaguchi-shi, Saitama 332 0012, Japan.
5: CREST, Japan Agency for Medical Research and Development (AMED), 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100 0004, Japan.

Articles citing this

A Rationally Designed Reversible 'Turn-Off' Sensor for Glutathione. Biosensors (Basel) (2017) 0.75

Quantitative real-time imaging of glutathione. Nat Commun (2017) 0.75

Articles cited by this

Glutathione. Annu Rev Biochem (1983) 15.18

Glutathione metabolism and its implications for health. J Nutr (2004) 6.27

Reactive oxygen species, antioxidants, and the mammalian thioredoxin system. Free Radic Biol Med (2001) 5.38

Rapid cancer detection by topically spraying a γ-glutamyltranspeptidase-activated fluorescent probe. Sci Transl Med (2011) 4.72

The importance of glutathione in human disease. Biomed Pharmacother (2003) 3.61

CD44 variant regulates redox status in cancer cells by stabilizing the xCT subunit of system xc(-) and thereby promotes tumor growth. Cancer Cell (2011) 3.41

Real-time imaging of the intracellular glutathione redox potential. Nat Methods (2008) 3.03

Glutathione in cancer biology and therapy. Crit Rev Clin Lab Sci (2006) 3.03

A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins. Nat Chem (2013) 2.61

The role of glutathione in cancer. Cell Biochem Funct (2004) 2.36

Increased levels of superoxide and H2O2 mediate the differential susceptibility of cancer cells versus normal cells to glucose deprivation. Biochem J (2009) 2.35

Mitochondrial glutathione, a key survival antioxidant. Antioxid Redox Signal (2009) 2.28

Mitochondrial O2*- and H2O2 mediate glucose deprivation-induced stress in human cancer cells. J Biol Chem (2004) 1.94

Glutathione monoethyl ester: preparation, uptake by tissues, and conversion to glutathione. Arch Biochem Biophys (1985) 1.82

Reference scales for the characterization of cationic electrophiles and neutral nucleophiles. J Am Chem Soc (2001) 1.73

A spontaneously blinking fluorophore based on intramolecular spirocyclization for live-cell super-resolution imaging. Nat Chem (2014) 1.61

Bright building blocks for chemical biology. ACS Chem Biol (2014) 1.44

Fluorescent and colorimetric probes for detection of thiols. Chem Soc Rev (2010) 1.44

Expression of gamma-glutamyltransferase in cancer cells and its significance in drug resistance. Biochem Pharmacol (2005) 1.39

Evolution of group 14 rhodamines as platforms for near-infrared fluorescence probes utilizing photoinduced electron transfer. ACS Chem Biol (2011) 1.38

Development of a highly specific rhodamine-based fluorescence probe for hypochlorous acid and its application to real-time imaging of phagocytosis. J Am Chem Soc (2007) 1.16

Combinatorial rosamine library and application to in vivo glutathione probe. J Am Chem Soc (2007) 1.06

In vivo assessment of cancerous tumors using boron doped diamond microelectrode. Sci Rep (2012) 1.05

BODIPY-based ratiometric fluorescent sensor for highly selective detection of glutathione over cysteine and homocysteine. J Am Chem Soc (2012) 1.04

Ratiometric fluorescence imaging of cellular glutathione. Org Lett (2011) 1.03

Cyanine-based fluorescent probe for highly selective detection of glutathione in cell cultures and live mouse tissues. J Am Chem Soc (2014) 0.94

Inhibition of oral cancer cell growth by adenovirusMnSOD plus BCNU treatment. Free Radic Biol Med (2003) 0.93

Tunable heptamethine-azo dye conjugate as an NIR fluorescent probe for the selective detection of mitochondrial glutathione over cysteine and homocysteine. J Am Chem Soc (2014) 0.92

Silicon-substituted xanthene dyes and their applications in bioimaging. Analyst (2015) 0.91

Increase in thiol oxidative stress via glutathione reductase inhibition as a novel approach to enhance cancer sensitivity to X-ray irradiation. Free Radic Biol Med (2009) 0.88

Quantitative imaging of glutathione in live cells using a reversible reaction-based ratiometric fluorescent probe. ACS Chem Biol (2015) 0.87

A practical guide for estimating rates of heterolysis reactions. Acc Chem Res (2010) 0.86

Rational design of highly sensitive fluorescence probes for protease and glycosidase based on precisely controlled spirocyclization. J Am Chem Soc (2012) 0.84

New trends in near-infrared fluorophores for bioimaging. Anal Sci (2014) 0.84

Glucose modulation induces reactive oxygen species and increases P-glycoprotein-mediated multidrug resistance to chemotherapeutics. Br J Pharmacol (2015) 0.82

A design concept of long-wavelength fluorescent analogs of rhodamine dyes: replacement of oxygen with silicon atom. Chem Commun (Camb) (2008) 0.81

Scales of Lewis basicities toward C-centered Lewis acids (carbocations). J Am Chem Soc (2015) 0.81

Theoretical and Experimental Investigation of Thermodynamics and Kinetics of Thiol-Michael Addition Reactions: A Case Study of Reversible Fluorescent Probes for Glutathione Imaging in Single Cells. Org Lett (2015) 0.81

Quantification of the electrophilic reactivities of aldehydes, imines, and enones. J Am Chem Soc (2011) 0.80

Rapid Cancer Fluorescence Imaging Using A γ-Glutamyltranspeptidase-Specific Probe For Primary Lung Cancer. Transl Oncol (2016) 0.76

A highly sensitive, cell-membrane-permeable fluorescent probe for glutathione. Bioorg Med Chem Lett (2014) 0.76