Efficient generation of mutations mediated by CRISPR/Cas9 in the hairy root transformation system of Brassica carinata.

PubWeight™: 0.75‹?›

🔗 View Article (PMID 28937992)

Published in PLoS One on September 22, 2017

Authors

Thomas W Kirchner1, Markus Niehaus1, Thomas Debener2, Manfred K Schenk1, Marco Herde3

Author Affiliations

1: Institute of Plant Nutrition, Leibniz Universitaet Hannover, Hannover, Germany.
2: Institute for Plant Genetics, Leibniz Universitaet Hannover, Hannover, Germany.
3: Leibniz University Hannover, Institute of Plant Nutrition, Department of Molecular Nutrition and Biochemistry of Plants, 30419 Hannover, Germany.

Articles cited by this

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol (2002) 82.19

Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res (2003) 77.89

Multiplex genome engineering using CRISPR/Cas systems. Science (2013) 55.53

A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science (2012) 48.17

Primer3--new capabilities and interfaces. Nucleic Acids Res (2012) 20.34

An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol (2000) 19.89

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nat Biotechnol (2013) 18.01

RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol (2013) 15.51

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence. Proc Natl Acad Sci U S A (2011) 6.89

Insulators: many functions, many mechanisms. Genes Dev (2002) 5.36

Rational design of highly active sgRNAs for CRISPR-Cas9-mediated gene inactivation. Nat Biotechnol (2014) 3.66

The biology of arabinogalactan proteins. Annu Rev Plant Biol (2007) 2.95

Multigeneration analysis reveals the inheritance, specificity, and patterns of CRISPR/Cas-induced gene modifications in Arabidopsis. Proc Natl Acad Sci U S A (2014) 2.26

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation. Plant Biotechnol J (2014) 1.93

Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana. Plant J (2014) 1.72

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Proc Natl Acad Sci U S A (2015) 1.68

The CRISPR/Cas9 system for plant genome editing and beyond. Biotechnol Adv (2014) 1.64

Large chromosomal deletions and heritable small genetic changes induced by CRISPR/Cas9 in rice. Nucleic Acids Res (2014) 1.58

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. Mol Plant (2015) 1.46

Editing plant genomes with CRISPR/Cas9. Curr Opin Biotechnol (2014) 1.41

Targeted genome modifications in soybean with CRISPR/Cas9. BMC Biotechnol (2015) 1.28

Hairy root transformation using Agrobacterium rhizogenes as a tool for exploring cell type-specific gene expression and function using tomato as a model. Plant Physiol (2014) 1.14

Advances in CRISPR-Cas9 genome engineering: lessons learned from RNA interference. Nucleic Acids Res (2015) 1.10

RNA interference as a gene knockdown technique. Int J Biochem Cell Biol (2009) 1.08

The promoter of the Vicia faba L. leghemoglobin gene VfLb29 is specifically activated in the infected cells of root nodules and in the arbuscule-containing cells of mycorrhizal roots from different legume and nonlegume plants. Mol Plant Microbe Interact (2004) 1.06

Induction of targeted, heritable mutations in barley and Brassica oleracea using RNA-guided Cas9 nuclease. Genome Biol (2015) 1.06

Green fluorescent protein as a marker for expression of a second gene in transgenic plants. Nat Biotechnol (1999) 0.98

CRISPR/Cas mutagenesis of soybean and Medicago truncatula using a new web-tool and a modified Cas9 enzyme. GM Crops Food (2015) 0.91

DNA recombination in somatic plant cells: mechanisms and evolutionary consequences. Chromosome Res (2014) 0.86

An endogene-resembling transgene delays the onset of silencing and limits siRNA accumulation. FEBS Lett (2013) 0.82

Research of methods to detect genomic mutations induced by CRISPR/Cas systems. J Biotechnol (2015) 0.80

Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases. Biotechnol Adv (2016) 0.80

Targeted Gene Manipulation in Plants Using the CRISPR/Cas Technology. J Genet Genomics (2016) 0.80

Efficient Inactivation of Symbiotic Nitrogen Fixation Related Genes in Lotus japonicus Using CRISPR-Cas9. Front Plant Sci (2016) 0.79

Recent Advances in Genome Editing Using CRISPR/Cas9. Front Plant Sci (2016) 0.76