Published in Methods Enzymol on January 01, 1986
Thermal motions of surface alpha-helices in the D-galactose chemosensory receptor. Detection by disulfide trapping. J Mol Biol (1992) 2.98
Epidermal growth factor binding induces a conformational change in the external domain of its receptor. EMBO J (1989) 1.56
Molecular motions of a glycopeptide from human serum transferrin studied by 13C nuclear magnetic resonance. Biophys J (1997) 0.75
Analysis of fluorescence decay kinetics from variable-frequency phase shift and modulation data. Biophys J (1984) 4.70
Quenching of protein fluorescence by oxygen. Detection of structural fluctuations in proteins on the nanosecond time scale. Biochemistry (1973) 4.28
Correction of timing errors in photomultiplier tubes used in phase-modulation fluorometry. J Biochem Biophys Methods (1981) 4.13
Functionally different agonists induce distinct conformations in the G protein coupling domain of the beta 2 adrenergic receptor. J Biol Chem (2001) 3.41
Differential polarized phase fluorometric investigations of diphenylhexatriene in lipid bilayers. Quantitation of hindered depolarizing rotations. Biochemistry (1979) 2.48
Quenching of fluorescence by oxygen. A probe for structural fluctuations in macromolecules. Biochemistry (1973) 2.48
Resolution of mixtures of fluorophores using variable-frequency phase and modulation data. Biophys J (1984) 1.87
A FRET-based sensor reveals large ATP hydrolysis-induced conformational changes and three distinct states of the molecular motor myosin. Cell (2000) 1.75
Glucose sensor for low-cost lifetime-based sensing using a genetically engineered protein. Anal Biochem (1999) 1.62
Quantitation of hindered rotations of diphenylhexatriene in lipid bilayers by differential polarized phase fluorometry. Science (1978) 1.57
Rotational freedom of tryptophan residues in proteins and peptides. Biochemistry (1983) 1.54
Dipolar relaxation in proteins on the nanosecond timescale observed by wavelength-resolved phase fluorometry of tryptophan fluorescence. J Biol Chem (1980) 1.53
Construction and performance of a variable-frequency phase-modulation fluorometer. Biophys Chem (1985) 1.37
Nanosecond segmental mobilities of tryptophan residues in proteins observed by lifetime-resolved fluorescence anisotropies. Biophys J (1980) 1.32
On the possibility of calcium imaging using Indo-1 with three-photon excitation. Photochem Photobiol (1995) 1.31
Optical measurements of pH using fluorescence lifetimes and phase-modulation fluorometry. Anal Chem (1993) 1.30
Fluorescence spectroscopic investigations of the dynamic properties of proteins, membranes and nucleic acids. J Biochem Biophys Methods (1980) 1.27
Asbestos-mediated membrane uptake of benzo[a]pyrene observed by fluorescence spectroscopy. Nature (1978) 1.26
Resolution of heterogeneous fluorescence from proteins and aromatic amino acids by phase-sensitive detection of fluorescence. J Biol Chem (1981) 1.20
Mechanisms for the biomethylation of metals and metalloids. Fed Proc (1978) 1.19
Frequency domain imaging of absorbers obscured by scattering. J Photochem Photobiol B (1992) 1.19
Theory of light quenching: effects of fluorescence polarization, intensity, and anisotropy decays. Biophys J (1994) 1.16
Fluorescence anisotropy measurements under oxygen quenching conditions as a method to quantify the depolarizing rotations of fluorophores. Application to diphenylhexatriene in isotropic solvents and in lipid bilayers. Biochemistry (1979) 1.16
Fluorescence of reduced nicotinamides using one- and two-photon excitation. Biophys Chem (1996) 1.11
Phase-resolved fluorescence lifetime measurements for flow cytometry. Cytometry (1993) 1.11
The aggregation state of mellitin in lipid bilayers. An energy transfer study. J Biol Chem (1986) 1.11
A thermophilic apoglucose dehydrogenase as nonconsuming glucose sensor. Biochem Biophys Res Commun (2000) 1.09
Phase-sensitive fluorescence spectroscopy: a new method to resolve fluorescence lifetimes or emission spectra of components in a mixture of fluorophores. J Biochem Biophys Methods (1981) 1.09
Metal-ligand complexes as a new class of long-lived fluorophores for protein hydrodynamics. Biophys J (1995) 1.09
Direct recording of the initially excited and the solvent relaxed fluorescence emission spectra of tryptophan by phase sensitive detection of fluorescence. Photochem Photobiol (1982) 1.07
Resolution of end-to-end distance distributions of flexible molecules using quenching-induced variations of the Forster distance for fluorescence energy transfer. Biophys J (1988) 1.06
Time-resolved fluorescence anisotropies of diphenylhexatriene and perylene in solvents and lipid bilayers obtained from multifrequency phase-modulation fluorometry. Biochemistry (1985) 1.06
New color chemosensors for monosaccharides based on azo dyes. Org Lett (2001) 1.06
Long-lifetime Ru(II) complexes as labeling reagents for sulfhydryl groups. Anal Biochem (1998) 1.05
Measurement of subnanosecond anisotropy decays of protein fluorescence using frequency-domain fluorometry. J Biol Chem (1986) 1.05
Frequency-domain fluorescence microscopy with the LED as a light source. J Microsc (2001) 1.05
Distance distributions and anisotropy decays of troponin C and its complex with troponin I. Biochemistry (1991) 1.04
Conformation heterogeneity in proteins as an origin of heterogeneous fluorescence decays, illustrated by native and denatured ribonuclease T1. Biochim Biophys Acta (1988) 1.04
Synthesis and evaluation of Ru-complexes as anisotropy probes for protein hydrodynamics and immunoassays of high-molecular-weight antigens. Biophys Chem (1996) 1.03
A long-lived, highly luminescent Re(I) metal-ligand complex as a biomolecular probe. Anal Biochem (1997) 1.03
Three-photon excitation in fluorescence microscopy. J Biomed Opt (1996) 1.03
Fluorescence lifetime characterization of novel low-pH probes. Anal Biochem (2001) 1.03
The fluorescence emission of the apo-glucose oxidase from Aspergillus niger as probe to estimate glucose concentrations. Biochem Biophys Res Commun (1999) 1.02
Inhibitory region of troponin I: Ca(2+)-dependent structural and environmental changes in the troponin-tropomyosin complex and in reconstituted thin filaments. Biochemistry (2000) 1.02
Differential-wavelength deconvolution of time-resolved fluorescence intensities. A new method for the analysis of excited-state processes. Biophys Chem (1982) 1.02
Three-photon induced fluorescence of the calcium probe Indo-1. Biophys J (1996) 1.02
Fluorescence lifetime-based sensing in tissues: a computational study. Biophys J (1995) 1.01
Theory of phase-modulation fluorescence spectroscopy for excited-state processes. Biophys Chem (1982) 1.01
Optical determination of glutamine using a genetically engineered protein. Anal Biochem (2001) 1.00
Long-wavelength long-lifetime luminophores. Anal Chem (2001) 0.99
Lifetime-based pH sensors: indicators for acidic environments. Anal Biochem (1999) 0.99
Evaluation of two synthetic glucose probes for fluorescence-lifetime-based sensing. Anal Biochem (2001) 0.98
Effects of temperature on the fluorescence intensity and anisotropy decays of staphylococcal nuclease and the less stable nuclease-conA-SG28 mutant. Biochemistry (1991) 0.98
Picosecond resolution of tyrosine fluorescence and anisotropy decays by 2-GHz frequency-domain fluorometry. Biochemistry (1987) 0.98
Binding of an indole derivative to micelles as quantified by phase-sensitive detection of fluorescence. J Biol Chem (1983) 0.97
Diffusion and partitioning of a pesticide, lindane, into phosphatidylcholine bilayers. A new fluorescence quenching method to study chlorinated hydrocarbon-membrane interactions. Biochim Biophys Acta (1977) 0.97
Possibility of simultaneously measuring low and high calcium concentrations using Fura-2 and lifetime-based sensing. Cell Calcium (1995) 0.96
Transport of a carcinogen, benzo[a]pyrene, from particulates to lipid bilayers: a model for the fate of particle-adsorbed polynuclear aromatic hydrocarbons which are retained in the lungs. Biochim Biophys Acta (1980) 0.96
Benzo[a]pyrene uptake into rat liver microsomes: effects of adsorption of benzo[a]pyrene to asbestos and non-fibrous mineral particulates. Chem Biol Interact (1980) 0.96
Frequency-domain reflectance for the determination of the scattering and absorption properties of tissue. Appl Opt (1991) 0.95
Sensing oxygen through skin using a red diode laser and fluorescence lifetimes. Biosens Bioelectron (1995) 0.95
Interactions of chlorinated hydrocarbon insecticides with membranes. Biochim Biophys Acta (1982) 0.95
A water-soluble luminescence oxygen sensor. Photochem Photobiol (1998) 0.94
Red-edge excitation of fluorescence and dynamic properties of proteins and membranes. Biochemistry (1984) 0.94
Determination of time-resolved fluorescence emission spectra and anisotropies of a fluorophore-protein complex using frequency-domain phase-modulation fluorometry. J Biol Chem (1984) 0.94
Fluorescence energy transfer in one dimension: frequency-domain fluorescence study of DNA-fluorophore complexes. Biopolymers (1995) 0.94
Use of a long-lifetime Re(I) complex in fluorescence polarization immunoassays of high-molecular-weight analytes. Anal Chem (1998) 0.94
Calcium-dependent fluorescence lifetimes of Indo-1 for one- and two-photon excitation of fluorescence. Photochem Photobiol (1993) 0.94
Interaction of SecB with soluble SecA. FEBS Lett (1997) 0.93
Principles of frequency-domain fluorescence spectroscopy and applications to cell membranes. Subcell Biochem (1988) 0.93
Analysis of excited-state processes by phase-modulation fluorescence spectroscopy. Biophys Chem (1982) 0.93
The beta-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: enzyme activity and conformational dynamics at temperatures above 100 degrees C. Biophys Chem (1999) 0.93
Thermodynamics and dynamics of histidine-binding protein, the water-soluble receptor of histidine permease. Implications for the transport of high and low affinity ligands. Eur J Biochem (2000) 0.92
Lifetime-based sensing of glucose using energy transfer with a long lifetime donor. Anal Biochem (1997) 0.92
Enhanced resolution of fluorescence anisotropy decays by simultaneous analysis of progressively quenched samples. Applications to anisotropic rotations and to protein dynamics. Biophys J (1987) 0.91
Time-resolved energy transfer measurements of donor-acceptor distance distributions and intramolecular flexibility of a CCHH zinc finger peptide. Biochemistry (1993) 0.91
Oxygen quenching and fluorescence depolarization of tyrosine residues in proteins. J Biol Chem (1983) 0.90
Fluorescence lifetime imaging of nuclear DNA: effect of fluorescence resonance energy transfer. Cytometry (2000) 0.90
A study of protein dynamics from anisotropy decays obtained by variable frequency phase-modulation fluorometry: internal motions of N-methylanthraniloyl melittin. Biochim Biophys Acta (1986) 0.90
Two-photon excitation of ethidium bromide labeled DNA. Biophys Chem (1997) 0.90
Fluorescence energy transfer immunoassay based on a long-lifetime luminescent metal-ligand complex. Anal Biochem (1995) 0.90
Enzyme fluorescence as a sensing tool: new perspectives in biotechnology. Curr Opin Biotechnol (2001) 0.89
Light quenching of tetraphenylbutadiene fluorescence observed during two-photon excitation. J Fluoresc (1993) 0.89
Effects of asbetos, iron oxide, silica, and carbon black on the microsomal availability of benzo[a]pyrene. Biochemistry (1979) 0.89
Chlorinated hydrocarbon-cell membrane interactions studied by the fluorescence quenching of carbazole-labeled phospholipids: probe synthesis and characterization of the quenching methodology. Chem Phys Lipids (1980) 0.89
Fluorescence properties of rhodamine 800 in whole blood and plasma. Anal Biochem (2000) 0.89
Demonstration of an associated anisotropy decay by frequency-domain fluorometry. Biophys Chem (1987) 0.89
Light quenching and depolarization of fluorescence observed with laser pulses. A new experimental opportunity in time-resolved fluorescence spectroscopy. Biophys Chem (1994) 0.88
Distance distributions in native and random-coil troponin I from frequency-domain measurements of fluorescence energy transfer. Biopolymers (1988) 0.88
Synthesis of squaraine-N-hydroxysuccinimide esters and their biological application as long-wavelength fluorescent labels. Anal Biochem (1994) 0.87
Time-resolved and steady-state fluorescence quenching of N-acetyl-L-tryptophanamide by acrylamide and iodide. Biophys Chem (1998) 0.87
Resolution of the lifetimes and correlation times of the intrinsic tryptophan fluorescence of human hemoglobin solutions using 2 GHz frequency-domain fluorometry. J Biol Chem (1988) 0.87
Fluorescence polarization immunoassay of a high-molecular-weight antigen based on a long-lifetime Ru-ligand complex. Anal Biochem (1995) 0.87
Hindered depolarizing rotations of perylene in lipid bilayers. Detection by lifetime-resolved fluorescence anisotropy measurements. Biochemistry (1980) 0.87
Time-resolved emission spectra of hemoglobin on the picosecond time scale. Biophys Chem (1988) 0.86
Two-photon excitation by the evanescent wave from total internal reflection. Anal Biochem (1997) 0.86
Structural perturbation of the transmembrane region interferes with calcium binding by the Ca2+ transport ATPase. J Biol Chem (1991) 0.85
Calcium- and magnesium-dependent interactions between the C-terminus of troponin I and the N-terminal, regulatory domain of troponin C. Arch Biochem Biophys (2001) 0.84
Polarization-based oxygen sensor. Analyst (1999) 0.84
Effect of pressure on the self-association of melittin. Biochemistry (1984) 0.84