Protein-blotting on Polybrene-coated glass-fiber sheets. A basis for acid hydrolysis and gas-phase sequencing of picomole quantities of protein previously separated on sodium dodecyl sulfate/polyacrylamide gel.

PubWeight™: 1.90‹?› | Rank: Top 3%

🔗 View Article (PMID 3899644)

Published in Eur J Biochem on October 01, 1985

Authors

J Vandekerckhove, G Bauw, M Puype, J Van Damme, M Van Montagu

Articles citing this

Characterization of the herbicide-resistance gene bar from Streptomyces hygroscopicus. EMBO J (1987) 3.33

cDNA cloning reveals that the major group rhinovirus receptor on HeLa cells is intercellular adhesion molecule 1. Proc Natl Acad Sci U S A (1989) 3.09

A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope. J Bacteriol (1989) 2.89

Alterations in the phenotype of plant cells studied by NH(2)-terminal amino acid-sequence analysis of proteins electroblotted from two-dimensional gel-separated total extracts. Proc Natl Acad Sci U S A (1987) 2.79

Primary structure and processing of lysosomal alpha-glucosidase; homology with the intestinal sucrase-isomaltase complex. EMBO J (1988) 2.44

Escherichia coli host factor for site-specific DNA inversion: cloning and characterization of the fis gene. Proc Natl Acad Sci U S A (1988) 1.93

Human skin fibroblast stromelysin: structure, glycosylation, substrate specificity, and differential expression in normal and tumorigenic cells. Proc Natl Acad Sci U S A (1987) 1.89

Protein-electroblotting and -microsequencing strategies in generating protein data bases from two-dimensional gels. Proc Natl Acad Sci U S A (1989) 1.87

Spiroplasma virus 4: nucleotide sequence of the viral DNA, regulatory signals, and proposed genome organization. J Bacteriol (1987) 1.63

Molecular biologist's guide to proteomics. Microbiol Mol Biol Rev (2002) 1.45

Acanthamoeba actin and profilin can be cross-linked between glutamic acid 364 of actin and lysine 115 of profilin. J Cell Biol (1989) 1.38

Unique amino acid substitutions in the capsid proteins of foot-and-mouth disease virus from a persistent infection in cell culture. J Virol (1990) 1.35

Location of a highly conserved neutralizing epitope in the F glycoprotein of human respiratory syncytial virus. J Virol (1990) 1.26

The speciation of the proteome. Chem Cent J (2008) 1.21

Leuconostoc lactis beta-galactosidase is encoded by two overlapping genes. J Bacteriol (1992) 1.16

Structural phosphoprotein M2-1 of the human respiratory syncytial virus is an RNA binding protein. J Virol (2000) 1.12

Topographic localization of the heparin-binding domain of the neural cell adhesion molecule N-CAM. J Cell Biol (1986) 0.99

Human kidney amiloride-binding protein: cDNA structure and functional expression. Proc Natl Acad Sci U S A (1990) 0.90

Prevention of C-terminal autoprocessing of Lactococcus lactis SK11 cell-envelope proteinase by engineering of an essential surface loop. Biochem J (1994) 0.87

Purification and immunochemical characterization of a dihydrofolate reductase-thymidylate synthase enzyme complex from wild-carrot cells. EMBO J (1987) 0.86

Blot-sequencing of antibodies: application to analysis of V gene usage among anti-steroid monoclonal antibodies. Immunology (1991) 0.78

Induction of the major integral membrane protein of mouse liver peroxisomes by peroxisome proliferators. Biochem J (1992) 0.77

Electroblotting from Polyacrylamide Gels. Curr Protoc Protein Sci (2015) 0.75

Articles by these authors

Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci U S A (1988) 20.72

The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product. Cell (1990) 9.37

Control of Arabidopsis flower and seed development by the homeotic gene APETALA2. Plant Cell (1994) 8.32

Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucleic Acids Res (1985) 8.31

Supercoiled circular DNA in crown-gall inducing Agrobacterium strains. J Mol Biol (1974) 7.18

Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens. EMBO J (1983) 6.44

Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature (1998) 5.91

The complete amino acid sequence of actins from bovine aorta, bovine heart, bovine fast skeletal muscle, and rabbit slow skeletal muscle. A protein-chemical analysis of muscle actin differentiation. Differentiation (1979) 5.90

Nucleotide sequence and transcript map of the Agrobacterium tumefaciens Ti plasmid-encoded octopine synthase gene. J Mol Appl Genet (1982) 5.83

Superroot, a recessive mutation in Arabidopsis, confers auxin overproduction. Plant Cell (1995) 5.55

At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol (1978) 5.34

Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase. Nature (1985) 5.04

Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana. Nature (1999) 5.03

The functional role of the ELR motif in CXC chemokine-mediated angiogenesis. J Biol Chem (1995) 4.77

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis. Eur J Biochem (1988) 4.67

Genetic analysis of T-DNA transcripts in nopaline crown galls. Cell (1983) 4.66

Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose. J Cell Biol (1994) 4.61

Interleukin-6 in synovial fluid and serum of patients with rheumatoid arthritis and other inflammatory arthritides. Arthritis Rheum (1988) 4.60

Identification of the human 26-kD protein, interferon beta 2 (IFN-beta 2), as a B cell hybridoma/plasmacytoma growth factor induced by interleukin 1 and tumor necrosis factor. J Exp Med (1987) 4.58

Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715. Eur J Biochem (1986) 4.56

The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis. Proc Natl Acad Sci U S A (1997) 4.37

Tumor DNA structure in plant cells transformed by A. tumefaciens. Science (1980) 4.36

Genetic Identification of functions of TL-DNA transcripts in octopine crown galls. EMBO J (1982) 4.30

Characterization of different plaque-forming and defective temperate phages in Agrobacterium. J Gen Virol (1975) 4.12

Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from agrobacterium to the plant genome. Cell (1984) 4.09

Scatter factor: molecular characteristics and effect on the invasiveness of epithelial cells. J Cell Biol (1990) 4.05

Dominant negative mutants of the Cdc2 kinase uncouple cell division from iterative plant development. EMBO J (1995) 3.92

cdc2a expression in Arabidopsis is linked with competence for cell division. Plant Cell (1993) 3.86

Cloning and sequence of two different cDNAs encoding 1-aminocyclopropane-1-carboxylate synthase in tomato. Proc Natl Acad Sci U S A (1990) 3.85

Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector. Nature (1984) 3.77

GRASP65, a protein involved in the stacking of Golgi cisternae. Cell (1997) 3.75

Acquisition of tumour-inducing ability by non-oncogenic agrobacteria as a result of plasmid transfer. Nature (1975) 3.73

Expression of foreign genes in regenerated plants and in their progeny. EMBO J (1984) 3.67

Dual action of the active oxygen species during plant stress responses. Cell Mol Life Sci (2000) 3.64

Inhibition of T-tropic HIV strains by selective antagonization of the chemokine receptor CXCR4. J Exp Med (1997) 3.62

Rapid mapping of transposon insertion and deletion mutations in the large Ti-plasmids of Agrobacterium tumefaciens. Nucleic Acids Res (1979) 3.55

Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. EMBO J (1991) 3.48

PlantCARE, a plant cis-acting regulatory element database. Nucleic Acids Res (1999) 3.43

Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens. J Mol Biol (1981) 3.40

Light regulation of plant gene expression by an upstream enhancer-like element. Nature (1986) 3.37

Prefoldin, a chaperone that delivers unfolded proteins to cytosolic chaperonin. Cell (1998) 3.33

Strong cellular preference in the expression of a housekeeping gene of Arabidopsis thaliana encoding S-adenosylmethionine synthetase. Plant Cell (1989) 3.32

Elevated tumor necrosis factor alpha and interleukin-6 serum levels as markers for complicated Plasmodium falciparum malaria. Am J Med (1989) 3.32

Site-specific mutagenesis of Agrobacterium Ti plasmids and transfer of genes to plant cells. J Mol Appl Genet (1981) 3.31

Gene discovery in the wood-forming tissues of poplar: analysis of 5, 692 expressed sequence tags. Proc Natl Acad Sci U S A (1998) 3.31

Introduction of genetic material into plant cells. Science (1983) 3.29

Catalase is a sink for H2O2 and is indispensable for stress defence in C3 plants. EMBO J (1997) 3.29

Evidence for the identity of human scatter factor and human hepatocyte growth factor. Proc Natl Acad Sci U S A (1991) 3.27

Differential regulation of superoxide dismutases in plants exposed to environmental stress. Plant Cell (1991) 3.17

Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mapping. Plasmid (1980) 3.16

Suppression of beta-1,3-glucanase transgene expression in homozygous plants. EMBO J (1992) 3.14

Ti plasmids of Agrobacterium as conjugative plasmids. Nature (1977) 3.11

Neutrophil gelatinase B potentiates interleukin-8 tenfold by aminoterminal processing, whereas it degrades CTAP-III, PF-4, and GRO-alpha and leaves RANTES and MCP-2 intact. Blood (2000) 3.06

Restriction endonuclease mapping of the octopine tumor-inducing plasmid pTiAch5 of Agrobacterium tumefaciens. Plasmid (1981) 3.00

Vascular smooth muscle cells differ from other smooth muscle cells: predominance of vimentin filaments and a specific alpha-type actin. Proc Natl Acad Sci U S A (1981) 2.94

The induction of manganese superoxide dismutase in response to stress in Nicotiana plumbaginifolia. EMBO J (1989) 2.93

Homogeneous interferon-inducing 22K factor is related to endogenous pyrogen and interleukin-1. Nature (1985) 2.87

IL-1 stimulates IL-6 production in endothelial cells. J Immunol (1989) 2.85

The functional organization of the octopine Agrobacterium tumefaciens plasmid pTiB6s3. Plasmid (1981) 2.84

Mammalian cytoplasmic actins are the products of at least two genes and differ in primary structure in at least 25 identified positions from skeletal muscle actins. Proc Natl Acad Sci U S A (1978) 2.84

An Agrobacterium-transformed cell culture from the monocot Asparagus officinalis. EMBO J (1984) 2.82

Alterations in the phenotype of plant cells studied by NH(2)-terminal amino acid-sequence analysis of proteins electroblotted from two-dimensional gel-separated total extracts. Proc Natl Acad Sci U S A (1987) 2.79

Induction of a 26-kDa-protein mRNA in human cells treated with an interleukin-1-related, leukocyte-derived factor. Eur J Biochem (1985) 2.77

Internal organization, boundaries and integration of Ti-plasmid DNA in nopaline grown gall tumours. J Mol Biol (1980) 2.73

Illegitimate recombination in plants: a model for T-DNA integration. Genes Dev (1991) 2.73

Posttranscriptional silencing of reporter transgenes in tobacco correlates with DNA methylation. Proc Natl Acad Sci U S A (1994) 2.73

Determination of the Processing Sites of an Arabidopsis 2S Albumin and Characterization of the Complete Gene Family. Plant Physiol (1988) 2.70

Conserved motifs in a divergent nod box of Azorhizobium caulinodans ORS571 reveal a common structure in promoters regulated by LysR-type proteins. Proc Natl Acad Sci U S A (1992) 2.67

Response of serum interleukin-6 in patients undergoing elective surgery of varying severity. Clin Sci (Lond) (1990) 2.67

Gelatinase B functions as regulator and effector in leukocyte biology. J Leukoc Biol (2001) 2.66

Characterization of iron superoxide dismutase cDNAs from plants obtained by genetic complementation in Escherichia coli. Proc Natl Acad Sci U S A (1990) 2.59

Coexpression of a mutant beta-actin and the two normal beta- and gamma-cytoplasmic actins in a stably transformed human cell line. Cell (1980) 2.58

Characterization of a rice gene showing organ-specific expression in response to salt stress and drought. Plant Cell (1990) 2.57

Pathway leading to correctly folded beta-tubulin. Cell (1996) 2.55

The complete nucleotide sequence of the TL-DNA of the Agrobacterium tumefaciens plasmid pTiAch5. EMBO J (1984) 2.55

Redox-activated expression of the cytosolic copper/zinc superoxide dismutase gene in Nicotiana. Proc Natl Acad Sci U S A (1993) 2.55

Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex. Curr Biol (2001) 2.54

Identification of sequences involved in the polyadenylation of higher plant nuclear transcripts using Agrobacterium T-DNA genes as models. EMBO J (1983) 2.53

The ROOT MERISTEMLESS1/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. Plant Cell (2000) 2.51

Defense activation and enhanced pathogen tolerance induced by H2O2 in transgenic tobacco. Proc Natl Acad Sci U S A (1998) 2.48

The Arabidopsis functional homolog of the p34cdc2 protein kinase. Plant Cell (1991) 2.44

Mendelian transmission of genes introduced into plants by the Ti plasmids of Agrobacterium tumefaciens. Mol Gen Genet (1981) 2.37

A binary vector for transferring genomic libraries to plants. Nucleic Acids Res (1986) 2.34

Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: Evidence for a strictly compartmentalized plant defense system. Proc Natl Acad Sci U S A (1989) 2.32

The amino acid sequence of Physarum actin. Nature (1978) 2.28

Interferon gamma, a mediator of lethal lipopolysaccharide-induced Shwartzman-like shock reactions in mice. J Exp Med (1990) 2.25

Proteomic analysis of arabidopsis seed germination and priming. Plant Physiol (2001) 2.24

A carrot somatic embryo mutant is rescued by chitinase. Plant Cell (1992) 2.24

CD26, let it cut or cut it down. Immunol Today (1999) 2.24

Mutagenesis by insertion of the drug resistance transposon Tn7 applied to the Ti plasmid of Agrobacterium tumefaciens. Plasmid (1978) 2.19

Size, location and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; common transcripts in octopine and nopaline tumors. Cell (1983) 2.19