A DNA-directed cell-free system for beta-galactosidase synthesis; characterization of the de novo synthesized enzyme and some aspects of the regulation of synthesis.

PubWeight™: 1.53‹?› | Rank: Top 4%

🔗 View Article (PMID 4191708)

Published in Cold Spring Harb Symp Quant Biol on January 01, 1969

Authors

G Zubay, D A Chambers

Articles by these authors

Mechanism of activation of catabolite-sensitive genes: a positive control system. Proc Natl Acad Sci U S A (1970) 9.37

In vitro synthesis of protein in microbial systems. Annu Rev Genet (1973) 7.77

Prolonged transcription in a cell-free system involving nuclei and cytoplasm. Proc Natl Acad Sci U S A (1974) 6.74

Purification and DNA-binding properties of the catabolite gene activator protein. Proc Natl Acad Sci U S A (1971) 4.43

The stimulatory effect of cyclic adenosine 3'5'-monophosphate on DNA-directed synthesis of beta-galactosidase in a cell-free system. Proc Natl Acad Sci U S A (1969) 3.98

Synthesis of an R plasmid protein associated with tetracycline resistance is negatively regulated. Proc Natl Acad Sci U S A (1976) 3.32

Detection and isolation of the repressor protein for the tryptophan operon of Escherichia coli. Proc Natl Acad Sci U S A (1972) 2.89

Differential sensitivity of gene expression in vitro to inhibitors of DNA gyrase. Proc Natl Acad Sci U S A (1979) 2.74

DNA-directed peptide synthesis. II. The synthesis of the alpha-fragment of the enzyme beta-galactosidase. Proc Natl Acad Sci U S A (1967) 2.32

Regulation of in vitro transcription of the tryptophan operon by purified RNA polymerase in the presence of partially purified repressor and tryptophan. Nat New Biol (1973) 2.20

An adenosine 3':5'-cyclic monophosphate-binding protein that acts on the transcription process. Proc Natl Acad Sci U S A (1971) 2.17

Cell-free studies on the regulation of the arabinose operon. Nat New Biol (1971) 2.09

Effects of consecutive AGG codons on translation in Escherichia coli, demonstrated with a versatile codon test system. J Bacteriol (1993) 2.05

Effects of guanosine tetraphosphate, guanosine pentaphosphate, and beta-gamma methylenyl-guanosine pentaphosphate on gene expression of Escherichia coli in vitro. Proc Natl Acad Sci U S A (1974) 1.98

Consecutive low-usage leucine codons block translation only when near the 5' end of a message in Escherichia coli. J Mol Biol (1995) 1.73

Cell-free synthesis of rat growth hormone. Proc Natl Acad Sci U S A (1973) 1.67

Effects of guanosine tetraphosphate on cell-free synthesis of Escherichia coli ribosomal RNA and other gene products. Proc Natl Acad Sci U S A (1975) 1.58

DNA-directed peptide synthesis. VI. Regulating the expression of the lac operon in a cell-free system. Proc Natl Acad Sci U S A (1969) 1.57

Prokaryotic coupled transcription-translation. Methods Enzymol (1983) 1.56

Characterization of "ecto-ATPase" of human blood platelets. Arch Biochem Biophys (1967) 1.55

The isolation and properties of CAP, the catabolite gene activator. Methods Enzymol (1980) 1.54

Tryptophanyl-tRNA and tryptophanyl-tRNA synthetase are not required for in vitro repression of the tryptophan operon. Nat New Biol (1973) 1.46

DNA directed peptide synthesis. V. The cell-free synthesis of a polypeptide with beta-galactosidase activity. Biochem Biophys Res Commun (1968) 1.43

DNA-directed peptide synthesis. 3. Repression of beta-galactosidase synthesis and inhibition of repressor by inducer in a cell-free system. Proc Natl Acad Sci U S A (1967) 1.40

Low-usage codons in Escherichia coli, yeast, fruit fly and primates. Gene (1991) 1.39

DNA-directed cell-free synthesis of biologically active transfer RNA: su + 3 tyrosyl-tRNA. Proc Natl Acad Sci U S A (1971) 1.35

A possible termination factor for transcription in Escherichia coli. Biochem Biophys Res Commun (1974) 1.33

DNA-directed peptide synthesis. 1. A comparison of T2 and Escherichi coli DNA-directed peptide synthesis in two cell-free systems. Biochim Biophys Acta (1967) 1.30

Cell-free coupled transcription-translation system for investigation of linear DNA segments. Proc Natl Acad Sci U S A (1980) 1.24

Characterization of a beta-galactosidase formed between a complementary protein and a peptide synthesized de novo. J Bacteriol (1969) 1.22

Observations on the configuration of nucleotides near the 3'-hydroxy end of adapter RNA. Biochem Biophys Res Commun (1964) 1.17

Clustering of low usage codons and ribosome movement. J Theor Biol (1994) 1.15

Evidence for the preferential binding of the catabolite gene activator protein (CAP) to DNA containing the lac promoter. Biochem Biophys Res Commun (1975) 1.15

Norepinephrine-mediated inhibition of antitumor cytotoxic T lymphocyte generation involves a beta-adrenergic receptor mechanism and decreased TNF-alpha gene expression. J Immunol (1999) 1.13

In vitro repression of n- -acetyl-L-ornithinase synthesis in Escherichia coli. Mol Gen Genet (1973) 1.09

Incorporation of labelled nucleotides and aggregation of human blood platelets. Thromb Diath Haemorrh (1966) 1.09

Mitomycin-induced lethality of Escherichia coli cells containing the ColE1 Plasmid: involvement of the kil gene. J Bacteriol (1985) 1.08

Involvement of arginine in in vitro repression of transcription of arginine genes C, B and H in Escherichia coli K 12. Biochem Biophys Res Commun (1976) 1.08

Alopecia areata treated with topical minoxidil. Arch Dermatol (1984) 1.05

Effects of bacteriophage T4-induced modification of Escherichia coli RNA polymerase on gene expression in vitro. Proc Natl Acad Sci U S A (1975) 1.04

In vitro synthesis and repression of argininosuccinase in Escherichia coli K12; partial purification of the arginine repressor. Mol Gen Genet (1976) 1.03

Cyclic GMP stimulates lymphocyte nucleic acid synthesis. Nature (1974) 1.03

The effect of cyclic nucleotides on purine biosynthesis and the induction of PRPP synthetase during lymphocyte activation. Cell (1974) 1.02

Platelet incorporation of labelled adenosine and adenosine diphosphate. Thromb Diath Haemorrh (1969) 1.01

Differential bacteriophage DNA replication after induction of a strain of Escherichia coli doubly lysogenic for phi80 and phi80dlac. Virology (1968) 1.00

Multicopy single-stranded DNAs with mismatched base pairs are mutagenic in Escherichia coli. Mol Microbiol (1994) 0.99

Colicin synthesis and cell death. J Bacteriol (1985) 0.99

Synthesis of the arabinose operon regulator protein in a cell-free system. Mol Gen Genet (1973) 0.97

Possible mechanism of aggregation of blood platelets by adenosine diphosphate. Nature (1966) 0.96

Epidermal proliferation of nude mouse skin, pig skin, and pig skin grafts. Failure of nude mouse skin to respond to the tumor promoter 12-O-tetradecanoyl phorbol 13-acetate. J Exp Med (1980) 0.94

Negative regulation of beta and beta' synthesis by RNA polymerase. Mol Gen Genet (1981) 0.92

Incorporation by blood platelets of adenosine diphosphate labelled with carbon-14. Nature (1965) 0.90

The messenger-directed synthesis of the alpha-fragment of the enzyme beta-galactosidase. Biochem Biophys Res Commun (1973) 0.90

The replication pattern of adenovirus DNA in vivo reproduced in vitro. Eur J Biochem (1980) 0.89

Nucleohistone as a primer for RNA synthesis. Proc Natl Acad Sci U S A (1965) 0.89

Platelet volume: effect of temperature and agents affecting platelet aggregation. Am J Physiol (1969) 0.87

Involved and uninvolved skin from psoriatic subjects: are they equally diseased? Assessment by skin transplanted to congenitally athymic (nude) mice. J Clin Invest (1981) 0.87

Inhibition of lymphocyte activation by catecholamines: evidence for a non-classical mechanism of catecholamine action. Immunology (1995) 0.86

A cross-sectional analysis of aspartate aminotransferase in human gingival crevicular fluid. J Periodontal Res (1991) 0.86

Binding of adenine and adenine-related compounds to the clay montmorillonite and the mineral hydroxylapatite. Orig Life Evol Biosph (1995) 0.84

Mouse hepatitis virus infection suppresses modulation of mouse spleen T-cell activation. Immunology (1992) 0.83

Direct effects of minoxidil on epidermal cells in culture. J Invest Dermatol (1984) 0.83

Purification and properties of the Drosophila zen protein. Mol Cell Biochem (1988) 0.82

Analysis of ColE1 expression in vitro after chromosome fragmentation. J Bacteriol (1983) 0.82

Aspartate aminotransferase increases in crevicular fluid during experimental periodontitis in beagle dogs. J Periodontol (1984) 0.81

A multi-center clinical trial of a new chairside test in distinguishing between diseased and healthy periodontal sites. II. Association between site type and test outcome before and after therapy. J Periodontol (1996) 0.81

The Thy-1 epidermal cell: perspective and prospective. Br J Dermatol (1985) 0.81

Resistance of adenoviral DNA replication to aphidicolin is dependent on the 72-kilodalton DNA-binding protein. J Virol (1982) 0.81

A multicenter clinical trial of PerioGard in distinguishing between diseased and healthy periodontal sites. (I). Study design, methodology and therapeutic outcome. J Clin Periodontol (1995) 0.81

Biochemical and autoradiographic studies of different RNA's: evidence that transfer RNA is chromosomal in origin. Proc Natl Acad Sci U S A (1965) 0.80

Inhibition of host DNA synthesis by adenovirus infection is reversed at elevated temperatures. J Virol (1981) 0.80

Mechanism of catecholamine-mediated destabilization of messenger RNA encoding Thy-1 protein in T-lineage cells. J Immunol (1998) 0.80

Cyclic nucleotide-binding proteins detected by photoaffinity labeling in nucleus and cytoplasm of bovine liver. Proc Natl Acad Sci U S A (1978) 0.80

The distribution of Langerhans cells and CD1a antigen in healthy and diseased human gingiva. Reg Immunol (1990) 0.80

In vivo immunomodulation by peripheral adrenergic and cholinergic agonists/antagonists in rat and mouse models. Ann N Y Acad Sci (2000) 0.80

Thy-1+ epidermal cells are not demonstrable in rat and human skin. J Invest Dermatol (1986) 0.80

Neurotransmitter suppression of the in vitro generation of a cytotoxic T lymphocyte response against the syngeneic MOPC-315 plasmacytoma. Cancer Immunol Immunother (1995) 0.80

The peculiar nature of codon usage in primates. Genet Eng (N Y) (1991) 0.79

Hydrocortisone regulates the dynamics of plasminogen activator and plasminogen activator inhibitor expression in cultured murine keratinocytes. Exp Cell Res (1998) 0.79

Quantitative studies on the rate of reaction of adapter RNA with formaldehyde. Biochem Biophys Res Commun (1964) 0.78

Molecular mechanisms in proliferative skin disease. J Invest Dermatol (1976) 0.78

Cell-free synthesis of SU + 3 tyrosyl transfer RNA: characterization of the 4S product. Arch Biochem Biophys (1973) 0.78

Heterogeneity of epidermal cells detected by the presence of Thy-1 antigen in athymic (nude) and normal Balb/c mice. Exp Cell Biol (1984) 0.78

Regulation of gene expression in plasmid ColE1: delayed expression of the kil gene. J Bacteriol (1988) 0.78

Association of gingival crevicular fluid aspartate aminotransferase levels with histopathology during ligature-induced periodontitis in the beagle dog. J Dent Res (1991) 0.77

Identification and characterization of the adenosine 3',5'-cyclic monophosphate binding proteins appearing during the development of Dictyostelium discoideum. Biochim Biophys Acta (1980) 0.77

Constancy of DNA organization of polymorphic and nonpolymorphic genes during development in Xenopus. Differentiation (1985) 0.77

Plasminogen activator in periodontal health and disease. Ann N Y Acad Sci (1992) 0.77

Cyclic AMP-binding proteins in human blood platelets detected by photoaffinity labelling. Biochim Biophys Acta (1982) 0.77

The dialogue between the brain and immune system involves not only the HPA-axis. Z Rheumatol (2000) 0.77

A deliberate hypotensive technique for decreasing blood loss during radical hysterectomy and pelvic lymphadenectomy. Am J Obstet Gynecol (1983) 0.76

Nucleoside phosphorylation: a feasible step in the prebiotic pathway to RNA. Orig Life Evol Biosph (1999) 0.76

Effects of retinoic acid on plasminogen activators in murine epidermal cell culture. Ann N Y Acad Sci (1992) 0.76

Molecular characterization of plasminogen activators in human gingival crevicular fluid. Arch Oral Biol (1995) 0.76

Collagen turnover in renal disease. Exp Nephrol (1995) 0.76

Plasminogen activator in human periodontal health and disease. Arch Oral Biol (1991) 0.75

On the nature of -galactosidase synthesized by DNA-directed cell-free system. Mol Gen Genet (1973) 0.75

An evaluation of the Hoffer-Osmond diagnostic test. J Clin Psychol (1966) 0.75

Effects of biological response modifiers on plasminogen activator activity in epidermal cell culture. Br J Dermatol (1984) 0.75