Published in Methods Enzymol on January 01, 1974
Selective enzyme purification by affinity chromatography. Proc Natl Acad Sci U S A (1968) 10.63
The use of the avidin-biotin complex as a tool in molecular biology. Methods Biochem Anal (1980) 6.48
Simian virus 40 DNA directs synthesis of authentic viral polypeptides in a linked transcription-translation cell-free system. Proc Natl Acad Sci U S A (1975) 4.98
Three-dimensional structures of avidin and the avidin-biotin complex. Proc Natl Acad Sci U S A (1993) 3.02
Distribution of end-to-end distances of oligopeptides in solution as estimated by energy transfer. Proc Natl Acad Sci U S A (1975) 2.50
Direct biochemical mapping of eukaryotic viral DNA by means of a linked transcription-translation cell-free system. Proc Natl Acad Sci U S A (1976) 2.17
Affinity chromatography of bovine pancreatic ribonuclease A. Eur J Biochem (1969) 2.11
A 25-kD inhibitor of actin polymerization is a low molecular mass heat shock protein. J Cell Biol (1991) 2.08
Introduction to avidin-biotin technology. Methods Enzymol (1990) 1.96
Protein biotinylation. Methods Enzymol (1990) 1.89
Covalent coupling of nucleotides to agarose for affinity chromatography. Biochim Biophys Acta (1973) 1.84
Genetic characterization of human immunodeficiency virus type 1 nef gene products translated in vitro and expressed in mammalian cells. J Virol (1991) 1.81
A general method for the specific isolation of peptides containing modified residues, using insoluble antibody columns. Biochemistry (1971) 1.70
Irreversible inhibition of biotin transport in yeast by biotinyl-p-nitrophenyl ester. Proc Natl Acad Sci U S A (1971) 1.66
Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Appl Environ Microbiol (1995) 1.57
The avidin-biotin complex in affinity cytochemistry. Methods Enzymol (1979) 1.53
A new approach (cyano-transfer) for cyanogen bromide activation of Sepharose at neutral pH, which yields activated resins, free of interfering nitrogen derivatives. Biochem Biophys Res Commun (1982) 1.52
Cloning of DNA complementary to the measles virus mRNA encoding nucleocapsid protein. Proc Natl Acad Sci U S A (1980) 1.50
The covalent binding of daunomycin and adriamycin to antibodies, with retention of both drug and antibody activities. Cancer Res (1975) 1.48
Single-step purification of avidine from egg white by affinity chromatography on biocytin-Sepharose columns. Biochem Biophys Res Commun (1968) 1.43
Avidin-biotin technology ten years on: has it lived up to its expectations? Trends Biochem Sci (1989) 1.42
Affinity cytochemistry: the localization of lectin and antibody receptors on erythrocytes via the avidin-biotin complex. FEBS Lett (1976) 1.41
Expression and biochemical characterization of human immunodeficiency virus type 1 nef gene product. J Virol (1990) 1.37
Insolubilized biotin for the purification of avidin. Methods Enzymol (1974) 1.36
Intravenous infusion of apolipoprotein E accelerates clearance of plasma lipoproteins in rabbits. J Clin Invest (1989) 1.35
Characterization of measles virus-specific proteins synthesized in vivo and in vitro from acutely and persistently infected cells. J Virol (1979) 1.31
Site-specific mutagenesis of human apolipoprotein E. Receptor binding activity of variants with single amino acid substitutions. J Biol Chem (1988) 1.31
A sensitive enzyme assay for biotin, avidin, and streptavidin. Anal Biochem (1986) 1.29
A general method for the isolation of labelled peptides from affinity-labelled proteins. Biochem Biophys Res Commun (1970) 1.24
Application of avidin-biotin technology to affinity-based separations. J Chromatogr (1990) 1.22
Double-stranded RNA-dependent RNase activity associated with human immunodeficiency virus type 1 reverse transcriptase. Proc Natl Acad Sci U S A (1992) 1.22
Biotin binding to avidin. Oligosaccharide side chain not required for ligand association. Biochem J (1987) 1.22
Applications of avidin-biotin technology: literature survey. Methods Enzymol (1990) 1.20
The use of carbodiimides in the preparation of immunizing conjugates. Methods Enzymol (1980) 1.20
Affinity chromatography. Methods Enzymol (1984) 1.18
Studies on the biotin-binding site of streptavidin. Tryptophan residues involved in the active site. Biochem J (1988) 1.18
Sodium dodecyl sulfate-polyacrylamide gel electrophoretic method for assessing the quaternary state and comparative thermostability of avidin and streptavidin. Electrophoresis (1996) 1.18
The mode of action of allicin: its ready permeability through phospholipid membranes may contribute to its biological activity. Biochim Biophys Acta (2000) 1.17
The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins. Biochim Biophys Acta (1998) 1.17
Preparation of ferritin-avidin conjugates by reductive alkylation for use in electron microscopic cytochemistry. J Histochem Cytochem (1976) 1.16
Reversibility of biotin-binding by selective modification of tyrosine in avidin. Biochem J (1996) 1.14
The quaternary structure of streptavidin in urea. J Biol Chem (1991) 1.14
Studies on the biotin-binding site of avidin. Tryptophan residues involved in the active site. Biochem J (1988) 1.13
Physical, chemical and immunological stability of CHO-derived hepatitis B surface antigen (HBsAg) particles. Vaccine (1999) 1.12
Human Mn superoxide dismutase cDNA sequence. Nucleic Acids Res (1987) 1.12
Cellular distribution of HIV type 1 Nef protein: identification of domains in Nef required for association with membrane and detergent-insoluble cellular matrix. AIDS Res Hum Retroviruses (1994) 1.11
Haloacetyl derivatives. Methods Enzymol (1977) 1.11
Biotin induces tetramerization of a recombinant monomeric avidin. A model for protein-protein interactions. J Biol Chem (2000) 1.11
Characterization of an inhibitor of actin polymerization in vinculin-rich fraction of turkey gizzard smooth muscle. Eur J Biochem (1988) 1.11
An improved method for the single-step purification of streptavidin. J Biochem Biophys Methods (1986) 1.11
Studies on the biotin-binding site of avidin. Lysine residues involved in the active site. Biochem J (1987) 1.10
Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain. FEBS Lett (1999) 1.10
Biocytin hydrazide--a selective label for sialic acids, galactose, and other sugars in glycoconjugates using avidin-biotin technology. Anal Biochem (1988) 1.10
Tissue distribution of avidin and streptavidin injected to mice. Effect of avidin carbohydrate, streptavidin truncation and exogenous biotin. Eur J Biochem (1990) 1.09
Characterization of the double stranded RNA dependent RNase activity associated with recombinant reverse transcriptases. Nucleic Acids Res (1992) 1.08
Cell-free synthesis of rat parotid preamylase. J Biol Chem (1979) 1.06
Recombinant NeutraLite avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific binding properties. FEBS Lett (2000) 1.06
Studies on the biotin-binding sites of avidin and streptavidin. Tyrosine residues are involved in the binding site. Biochem J (1990) 1.05
Foreword and introduction to the book (strept)avidin-biotin system. Biomol Eng (1999) 1.05
Direct visualization of redistribution and capping of fluorescent gangliosides on lymphocytes. J Cell Biol (1984) 1.04
The mode of adsorption of proteins to aliphatic and aromatic amines coupled to cyanogen bromide-activated agarose. Biochim Biophys Acta (1974) 1.04
Mapping of Escherichia coli ribosomal components involved in peptidyl transferase activity. Proc Natl Acad Sci U S A (1973) 1.04
Molecular analysis of the second template switch during reverse transcription of the HIV RNA template. Biochemistry (1996) 1.03
Postsecretory modifications of streptavidin. Biochem J (1989) 1.03
Biotin-containing reagents. Methods Enzymol (1990) 1.03
The action of staphylococcal nuclease on synthetic substrates. Biochemistry (1969) 1.02
Studies on the biotin-binding site of avidin. Minimized fragments that bind biotin. Biochem J (1991) 1.02
Effect of purified allicin, the major ingredient of freshly crushed garlic, on cancer cell proliferation. Nutr Cancer (2000) 1.01
Streptavidin contains an RYD sequence which mimics the RGD receptor domain of fibronectin. Biochem Biophys Res Commun (1990) 1.00
Nonradioactive hybridization probes prepared by the reaction of biotin hydrazide with DNA. Biochem Biophys Res Commun (1987) 1.00
3-(N-Maleimido-propionyl)biocytin: a versatile thiol-specific biotinylating reagent. Anal Biochem (1985) 0.99
A chemical approach for the localization of membrane sites involved in lymphocyte activation. Biochem Biophys Res Commun (1976) 0.99
A spectrophotometric assay for allicin and alliinase (Alliin lyase) activity: reaction of 2-nitro-5-thiobenzoate with thiosulfinates. Anal Biochem (1998) 0.99
The reaction of derivatives of tryptophan, tryptamine, and other indoles with 2-hydroxy-5-nitrobenzyl bromide (Koshland's reagent). J Am Chem Soc (1968) 0.99
Analysis of proteins and glycoproteins on blots. Methods Enzymol (1990) 0.99
Affinity labeling and cross-linking of the heavy and light chains of a myeloma protein with anti-2,4-dinitrophenyl activity. Biochemistry (1971) 0.99
Biotin-binding proteins: overview and prospects. Methods Enzymol (1990) 0.99
Selective labeling of sulfhydryls and disulfides on blot transfers using avidin-biotin technology: studies on purified proteins and erythrocyte membranes. Anal Biochem (1987) 0.98
Production of biologically active recombinant avidin in baculovirus-infected insect cells. Protein Expr Purif (1997) 0.98
Fluorescence studies with tryptophyl peptides. Biochemistry (1967) 0.98
Biotin binding changes the conformation and decreases tryptophan accessibility of streptavidin. J Protein Chem (1990) 0.97
Monoclonal anti-biotin antibodies simulate avidin in the recognition of biotin. FEBS Lett (1993) 0.97
Inhibition of tumor growth by poly(ethylene glycol) derivatives of anti-ErbB2 antibodies. Cancer Immunol Immunother (2000) 0.97
The epitopes for natural polyreactive antibodies are rich in proline. Proc Natl Acad Sci U S A (1997) 0.97
Membrane sialoglycolipids emerging as possible signal transducers for lymphocyte stimulation. J Immunol (1981) 0.96
A monomeric von Willebrand factor fragment, Leu-504--Lys-728, inhibits von Willebrand factor interaction with glycoprotein Ib-IX [corrected]. Proc Natl Acad Sci U S A (1992) 0.96
S-Allylmercaptoglutathione: the reaction product of allicin with glutathione possesses SH-modifying and antioxidant properties. Biochim Biophys Acta (2000) 0.95
High-level expression of enzymatically active human Cu/Zn superoxide dismutase in Escherichia coli. Proc Natl Acad Sci U S A (1986) 0.95
Discrimination of DNA polymerase and RNase H activities in reverse transcriptase of avian myeloblastosis virus. Biochemistry (1978) 0.95
The literature on affinity chromatography. Methods Enzymol (1974) 0.95
Conversion of protein kinase to a cyclic AMP independent form by affinity chromatography on N 6 -caproyl 3',5'-cyclic adenosine monophosphate-sepharose. Biochem Biophys Res Commun (1971) 0.95
Polymers coupled to agarose as stable and high capacity spacers. Methods Enzymol (1974) 0.94
Cloning of bovine growth hormone gene and its expression in bacteria. Nucleic Acids Res (1981) 0.94
The effect in vivo of chemotherapeutic drug--antibody conjugates in two murine experimental tumor systems. Int J Cancer (1978) 0.94
A spectrophotometric assay for soluble and immobilized N-hydroxysuccinimide esters. Anal Biochem (1982) 0.93
p-Diazobenzoyl biocytin--a new biotinylating reagent for the labeling of tyrosines and histidines in proteins. Biochem Biophys Res Commun (1986) 0.93