Published in Methods Enzymol on January 01, 1980
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res (1982) 13.52
Expression of glnA in Escherichia coli is regulated at tandem promoters. Proc Natl Acad Sci U S A (1985) 4.77
Characterization of the response of the Arabidopsis response regulator gene family to cytokinin. Plant Physiol (2000) 3.35
The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene. Nucleic Acids Res (1983) 2.94
The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability. EMBO J (2002) 2.62
Variation of levels of mRNA coding for antenna and reaction center polypeptides in Rhodopseudomonas capsulata in response to changes in oxygen concentration. J Bacteriol (1984) 2.29
Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc Natl Acad Sci U S A (1991) 2.23
Cloned restriction/modification system from Pseudomonas aeruginosa. Proc Natl Acad Sci U S A (1983) 2.05
High-mobility-group 1 protein mediates DNA bending as determined by ring closures. Proc Natl Acad Sci U S A (1993) 2.03
Phylogenetic analysis of the genus Borrelia: a comparison of North American and European isolates of Borrelia burgdorferi. J Bacteriol (1992) 2.02
A set of tRNAs that lack either the T psi C arm or the dihydrouridine arm: towards a minimal tRNA adaptor. EMBO J (1990) 1.99
Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate. Nucleic Acids Res (1985) 1.93
Nucleotide sequence preservation of human mitochondrial DNA. Proc Natl Acad Sci U S A (1985) 1.88
A 3-bp deletion in the rhodopsin gene in a family with autosomal dominant retinitis pigmentosa. Am J Hum Genet (1991) 1.88
Two improved promoter sequences for the beta-lactamase expression arising from a single base-pair substitution. Nucleic Acids Res (1984) 1.72
Ribonuclease H activities associated with viral reverse transcriptases are endonucleases. Proc Natl Acad Sci U S A (1989) 1.67
UV-induced formation of pyrimidine dimers in nucleosome core DNA is strongly modulated with a period of 10.3 bases. Proc Natl Acad Sci U S A (1987) 1.61
2-Substitution of adenine nucleotide analogues containing a bicyclo[3.1.0]hexane ring system locked in a northern conformation: enhanced potency as P2Y1 receptor antagonists. J Med Chem (2003) 1.57
The DNA sequence of the gene rpsA of Escherichia coli coding for ribosomal protein S1. Nucleic Acids Res (1982) 1.51
Human alpha-L-iduronidase: cDNA isolation and expression. Proc Natl Acad Sci U S A (1991) 1.48
Nucleotide sequence of the control regions for the glnA and glnL genes of Salmonella typhimurium. J Bacteriol (1983) 1.46
Involvement of bases 787-795 of Escherichia coli 16S ribosomal RNA in ribosomal subunit association. Proc Natl Acad Sci U S A (1986) 1.44
Organization of rRNA structural genes in the archaebacterium Thermoplasma acidophilum. Nucleic Acids Res (1982) 1.42
Parallel stranded duplex DNA. Nucleic Acids Res (1988) 1.30
Nucleotides in precursor tRNAs that are required intact for catalysis by RNase P RNAs. Nucleic Acids Res (1991) 1.26
Detection of rubella virus gene sequences by enzymatic amplification and direct sequencing of amplified DNA. J Clin Microbiol (1991) 1.25
Rat mammary gland fatty acid synthase: localization of the constituent domains and two functional polyadenylation/termination signals in the cDNA. Nucleic Acids Res (1989) 1.22
Probability of recovering pathogenic Escherichia coli from foods. Appl Environ Microbiol (1985) 1.21
Blood coagulation factor X mRNA encodes a single polypeptide chain containing a prepro leader sequence. Nucleic Acids Res (1984) 1.20
Recognition of DNA substrates by T4 bacteriophage polynucleotide kinase. Nucleic Acids Res (2004) 1.18
Molecular cloning and nucleotide sequence of the colonization factor antigen I gene of Escherichia coli. Infect Immun (1989) 1.15
The strands of both polarities of a small circular RNA from carnation self-cleave in vitro through alternative double- and single-hammerhead structures. Nucleic Acids Res (1992) 1.13
Structure of simian virus 40-adeno-associated virus recombinant genomes. J Virol (1985) 1.13
Complete cDNA sequence of human preceruloplasmin. Proc Natl Acad Sci U S A (1986) 1.13
Characterization of a third major SINE family of repetitive sequences in the galago genome. Nucleic Acids Res (1991) 1.13
Preferential incorporation of nucleoside analogs after template switching during human immunodeficiency virus reverse transcription. Antimicrob Agents Chemother (1994) 1.09
Identification of novel single-stranded d(TC)n binding proteins in several mammalian species. Nucleic Acids Res (1991) 1.09
Regulation of maltase synthesis in Saccharomyces carlsbergensis. J Bacteriol (1983) 1.02
Heterologous enzyme function in Escherichia coli and the selection of genes encoding the catalytic RNA subunit of RNase P. Proc Natl Acad Sci U S A (1987) 1.02
Complexes of viroids with histones and other proteins. Nucleic Acids Res (1985) 1.01
High-level expression of a chemically synthesized gene for human interferon-gamma using a prokaryotic expression vector. Proc Natl Acad Sci U S A (1984) 1.00
Restriction analysis and homology studies of the bovine adenovirus 7 genome. J Virol (1984) 1.00
The binding site for ribosomal protein L2 within 23S ribosomal RNA of Escherichia coli. EMBO J (1988) 0.99
Reverse transcription of avian myeloblastosis virus 35S RNA. Early synthesis of plus strand DNA of discrete size in reconstructed reactions. Nucleic Acids Res (1982) 0.99
Excision of uracil residues in DNA: mechanism of action of Escherichia coli and Micrococcus luteus uracil-DNA glycosylases. Nucleic Acids Res (1985) 0.97
Enzymes used in molecular biology: a useful guide. J Cell Commun Signal (2008) 0.95
Contrasting effects of single stranded DNA binding protein on the activity of uracil DNA glycosylase from Escherichia coli towards different DNA substrates. Nucleic Acids Res (1997) 0.93
Purification and Characterization of a 70-Kilodalton Polyadenylate-Binding Protein from Pea (Pisum sativum). Plant Physiol (1992) 0.93
Complete c-mos (rat) nucleotide sequence: presence of conserved domains in c-mos proteins. Nucleic Acids Res (1984) 0.93
Novel RNA polymerization reaction catalyzed by a group I ribozyme. EMBO J (1993) 0.93
Mouse DNA sequences complementary to small nuclear RNA U1. Nucleic Acids Res (1982) 0.89
Sequencing heavy- and light-chain variable genes of single B-hybridoma cells by total enzymatic amplification. Proc Natl Acad Sci U S A (1992) 0.87
Subset of hybrid eukaryotic proteins is exported by the type I secretion system of Erwinia chrysanthemi. J Bacteriol (2001) 0.84
Na/H antiporter mRNA expression in single nephron segments of rat kidney cortex. J Clin Invest (1991) 0.83
Construction of a new yeast cloning vector containing autonomous replication sequences from Candida utilis. J Bacteriol (1983) 0.81
A novel exocytoplasmic endonuclease from Streptomyces antibioticus. Biochem J (1995) 0.81
The sequence of a viroid from grapevine closely related to severe isolates of citrus exocortis viroid. Nucleic Acids Res (1987) 0.81
Perturbation of DNA hairpins containing the EcoRI recognition site by hairpin loops of varying size and composition: physical (NMR and UV) and enzymatic (EcoRI) studies. Nucleic Acids Res (1990) 0.80
Structural equivalence in the transcribed spacers of pre-rRNA transcripts in Schizosaccharomyces pombe. Nucleic Acids Res (1999) 0.79
Probing the alpha-sarcin region of Escherichia coli 23S rRNA with a cDNA oligomer. Nucleic Acids Res (1988) 0.79
Functional significance of intermediate cleavages in the 3'ETS of the pre-rRNA from Schizosaccharomyces pombe. Nucleic Acids Res (2003) 0.77
Transcription start sites and epigenetic analysis of the HSD17B10 proximal promoter. BMC Biochem (2013) 0.77
Purification and characterization of a nutritionally controlled endodeoxyribonuclease from Streptomyces glaucescens. Biochem J (1992) 0.77
Identification of defined sequences in domain V of E. coli 23S rRNA in the 50S subunit accessible for hybridization with complementary oligodeoxyribonucleotides. Nucleic Acids Res (1988) 0.75
Chemical synthesis of long RNAs with terminal 5'-phosphate groups. Chemistry (2017) 0.75
Reversal of bacteriophage T4 induced polynucleotide kinase action. Biochemistry (1973) 21.41
Physical characterization and simultaneous purification of bacteriophage T4 induced polynucleotide kinase, polynucleotide ligase, and deoxyribonucleic acid polymerase. Biochemistry (1973) 11.45
End labeling of enzymatically decapped mRNA. Nucleic Acids Res (1977) 6.58
Transpososomes: stable protein-DNA complexes involved in the in vitro transposition of bacteriophage Mu DNA. Cell (1987) 2.73
Structural aspects of a higher order nucleoprotein complex: induction of an altered DNA structure at the Mu-host junction of the Mu type 1 transpososome. EMBO J (1991) 2.46
In vitro and in vivo manipulations of bacteriophage Mu DNA: cloning of Mu ends and construction of mini-Mu's carrying selectable markers. Gene (1981) 2.21
Total synthesis of the structural gene for the precursor of a tyrosine suppressor transfer RNA from Escherichia coli. 1. General introduction. J Biol Chem (1976) 1.91
Left-handed DNA: from synthetic polymers to chromosomes. J Biomol Struct Dyn (1983) 1.90
Z* DNA, the left-handed helical form of poly[d(G-C)] in MgCl2-ethanol, is biologically active. EMBO J (1982) 1.90
Sequence analysis, expression, and conservation of Escherichia coli uracil DNA glycosylase and its gene (ung). J Biol Chem (1988) 1.80
End group labelling of RNA and double stranded DNA by phosphate exchange catalyzed by bacteriophage T4 induced polynucleotide kinase. Biochem Biophys Res Commun (1975) 1.80
Mn2+ and other transition metals at low concentration induce the right-to-left helical transformation of poly[d(G-C)]. EMBO J (1982) 1.67
A protein factor which reduces the negative supercoiling requirement in the Mu DNA strand transfer reaction is Escherichia coli integration host factor. J Biol Chem (1989) 1.66
Predominant end-products of prophage Mu DNA transposition during the lytic cycle are replicon fusions. J Mol Biol (1981) 1.66
The nucleotide sequence of the B gene of bacteriophage Mu. Nucleic Acids Res (1984) 1.51
Site-specific HU binding in the Mu transpososome: conversion of a sequence-independent DNA-binding protein into a chemical nuclease. Genes Dev (1993) 1.49
'Z-RNA'--a left-handed RNA double helix. Nature (1984) 1.48
Specificities and kinetics of uracil excision from uracil-containing DNA oligomers by Escherichia coli uracil DNA glycosylase. Biochemistry (1991) 1.46
Amplification and purification of the bacteriophage Mu encoded B transposition protein. J Biol Chem (1985) 1.43
Anatomy of a flexer-DNA complex inside a higher-order transposition intermediate. Cell (1996) 1.41
Generation of left-handed Z-DNA in solution and visualization in polytene chromosomes by immunofluorescence. Cold Spring Harb Symp Quant Biol (1983) 1.36
Primary structure of phage mu transposase: homology to mu repressor. Proc Natl Acad Sci U S A (1985) 1.30
Left-handed helical conformation of poly[d(A-m5C).d(G-T)]. Nature (1983) 1.27
The Mu transpositional enhancer can function in trans: requirement of the enhancer for synapsis but not strand cleavage. Cell (1992) 1.26
Immunoelectron microscopic analysis of the A, B, and HU protein content of bacteriophage Mu transpososomes. J Biol Chem (1990) 1.25
Action at a distance in Mu DNA transposition: an enhancer-like element is the site of action of supercoiling relief activity by integration host factor (IHF). EMBO J (1989) 1.24
T4 polynucleotide ligase catalyzed joining of short synthetic DNA duplexes at base-paired ends. Biochemistry (1978) 1.23
The bacteriophage Mu N gene encodes the 64-kDa virion protein which is injected with, and circularizes, infecting Mu DNA. J Biol Chem (1986) 1.21
A new set of Mu DNA transposition intermediates: alternate pathways of target capture preceding strand transfer. EMBO J (1997) 1.14
Transposition of phage Mu DNA. Curr Top Microbiol Immunol (1996) 1.11
Three-site synapsis during Mu DNA transposition: a critical intermediate preceding engagement of the active site. Cell (1996) 1.11
Identification of novel single-stranded d(TC)n binding proteins in several mammalian species. Nucleic Acids Res (1991) 1.09
A novel DNA binding and nuclease activity in domain III of Mu transposase: evidence for a catalytic region involved in donor cleavage. EMBO J (1995) 1.08
Stimulation of the Mu DNA strand cleavage and intramolecular strand transfer reactions by the Mu B protein is independent of stable binding of the Mu B protein to DNA. J Biol Chem (1991) 1.08
A truncated form of the bacteriophage Mu B protein promotes conservative integration, but not replicative transposition, of Mu DNA. Cell (1985) 1.07
Site-specific DNA binding and bending by the Borrelia burgdorferi Hbb protein. Mol Microbiol (2000) 1.06
Supercoiling-dependent site-specific binding of HU to naked Mu DNA. J Mol Biol (1999) 1.06
Right- and left-handed (Z) helical conformations of the hairpin d(C-G)5T4(C-G)5 monomer and dimer. Biochemistry (1985) 1.06
Polyadenylic acid sequences in the RNA of Hyphomicrobium. J Bacteriol (1978) 1.05
Uracil-DNA glycosylase as a probe for protein--DNA interactions. Nucleic Acids Res (1993) 1.05
Association of Mu-containing plasmids with the Escherichia coli chromosome upon prophage induction. Proc Natl Acad Sci U S A (1980) 1.05
Relative stability of parallel- and antiparallel-stranded duplex DNA. Biochemistry (1988) 1.04
Left-handed Z-DNA regions are present in negatively supercoiled bacteriophage PM2 DNA. EMBO J (1983) 1.02
A second high affinity HU binding site in the phage Mu transpososome. J Biol Chem (1994) 1.02
Mechanistic aspects of DNA transposition. Curr Opin Genet Dev (1992) 1.01
Role of the A protein-binding sites in the in vitro transposition of mu DNA. A complex circuit of interactions involving the mu ends and the transpositional enhancer. J Biol Chem (1992) 1.00
Polymerization of oligodeoxythymidylates and oligoriboadenylates catalyzed by T4 polynucleotide ligase and their use as analytical markers in polyacrylamide-gel electrophoresis. Anal Biochem (1976) 0.96
Flanking host sequences can exert an inhibitory effect on the cleavage step of the in vitro mu DNA strand transfer reaction. J Biol Chem (1992) 0.95
Length-dependent cruciform extrusion in d(GTAC)n sequences. J Biomol Struct Dyn (1988) 0.95
Predominant integration end products of infecting bacteriophage Mu DNA are simple insertions with no preference for integration of either Mu DNA strand. Virology (1983) 0.94
Characterization of a region in phage Mu transposase that is involved in interaction with the Mu B protein. J Biol Chem (1994) 0.93
Transposition of bacteriophage mu DNA: expression of the A and B proteins from lambda pL and analysis of infecting mu DNA. Cold Spring Harb Symp Quant Biol (1984) 0.93
Antibiotic induced electrophoretic mobility shifts of DNA restriction fragments. Nucleic Acids Res (1979) 0.92
Mechanism of bacteriophage Mu DNA transposition. Cold Spring Harb Symp Quant Biol (1981) 0.92
Assembly and characterization of nucleosomal cores on B- vs. Z-form DNA. Biochemistry (1985) 0.91
Solution structure of the parallel-stranded hairpin d(T8<text text>C4A8) as determined by two-dimensional NMR. Biochemistry (1993) 0.91
Comparison of the B- and Z-form hairpin loop structures formed by d(CG)5T4(CG)5. Biochemistry (1988) 0.90
Distribution of CT-rich tracts is conserved in vertebrate chromosomes. Chromosoma (1990) 0.89
Sequence of bacteriophage Mu N and P genes. Nucleic Acids Res (1988) 0.88
Covalently linked sequencing primer linkers (splinkers) for sequence analysis of restriction fragments. Gene (1986) 0.88
The mouse uracil-DNA glycosylase gene: isolation of cDNA and genomic clones and mapping ung to mouse chromosome 5. Gene (1997) 0.88
Are many Z-DNA binding proteins actually phospholipid-binding proteins? Proc Natl Acad Sci U S A (1990) 0.88
Nucleosome-core assembly on B and Z forms of poly[d(G-m5C)]. Cold Spring Harb Symp Quant Biol (1983) 0.87
Natural occurrence of left-handed (Z) regions in PM2 DNA. J Biomol Struct Dyn (1983) 0.86
Disruption of target DNA binding in Mu DNA transposition by alteration of position 99 in the Mu B protein. J Mol Biol (1998) 0.86
The circle is broken: telomere resolution in linear replicons. Curr Opin Microbiol (2001) 0.86
Mechanism of Mu DNA transposition. Bioessays (1988) 0.86
Effect of mutations in the Mu-host junction region on transpososome assembly. J Mol Biol (2001) 0.86
Yolk proteins from nematodes, chickens, and frogs bind strongly and preferentially to left-handed Z-DNA. J Biol Chem (1988) 0.85
Characterization of a parallel-stranded DNA hairpin. Biochemistry (1989) 0.85
Electron microscopic analysis of in vitro transposition intermediates of bacteriophage Mu DNA. Gene (1986) 0.85
Interaction of anthracyclines with DNA and chromosomes. Chromosoma (1978) 0.84
The solution structure of the C-terminal domain of the Mu B transposition protein. EMBO J (2000) 0.84
Structure of a DNA duplex that contains alpha-anomeric nucleotides and 3'-3' and 5'-5' phosphodiester linkages: coexistence of parallel and antiparallel DNA. Biochemistry (1996) 0.83
The effect of antibiotics on the T4 polynucleotide ligase catalyzed template dependent polymerization of oligodeoxythymidylates. Nucleic Acids Res (1979) 0.83
Mutations in domain III alpha of the Mu transposase: evidence suggesting an active site component which interacts with the Mu-host junction. J Mol Biol (1998) 0.83
Clearly differentiated and stable chromosome bands produced by a spermine bis-acridine, a bifunctional intercalating analogue of quinacrine. Exp Cell Res (1979) 0.83
The Mu transposase tetramer is inactive in unassisted strand transfer: an auto-allosteric effect of Mu A promotes the reaction in the absence of Mu B. J Mol Biol (1997) 0.82
Length-dependent formation of parallel-stranded DNA in alternating AT segments. Biochemistry (1990) 0.82
Synthesis and characterization of poly[d(G-z5C)]. B-Z transition and inhibition of DNA methylase. Biochemistry (1985) 0.81
Vibrational circular dichroism and IR absorption of DNA complexes with Cu2+ ions. Biopolymers (2003) 0.81
Interaction of Hoechst 33258 with repeating synthetic DNA polymers and natural DNA. J Biomol Struct Dyn (1988) 0.81
Perturbation of DNA hairpins containing the EcoRI recognition site by hairpin loops of varying size and composition: physical (NMR and UV) and enzymatic (EcoRI) studies. Nucleic Acids Res (1990) 0.80
Interaction of RecA protein with acidic phospholipids inhibits DNA-binding activity of RecA. J Bacteriol (1990) 0.80
Convenient methods to determine the specific radioactivity of (gamma-32P)ATP of high specific activity. Anal Biochem (1975) 0.80
Phosphorylation of N-protected deoxyoligonucleotides by T4 polynucleotide kinase. Biochemistry (1973) 0.80
A general method for the purification of synthetic oligodeoxyribonucleotides containing strong secondary structure by reversed-phase high-performance liquid chromatography on PRP-1 resin. Anal Biochem (1987) 0.79
Stress-induced cruciform formation in a cloned d(CATG)10 sequence. EMBO J (1986) 0.79
Structure of d(GT)n.d(GA)n sequences: formation of parallel stranded duplex DNA. Biochemistry (1998) 0.79
Reverse banding on chromosomes produced by a guanosine-cytosine specific DNA binding antibiotic: olivomycin. Science (1977) 0.79
Tandem repeats of a specific alternating purine-pyrimidine DNA sequence adjacent to protamine genes in the rainbow trout that can exist in the Z form. Biochemistry (1985) 0.79
Sequence analysis of a PM2-DNA anti-Z-IgG-binding region. Biosci Rep (1984) 0.77
NMR spectroscopic and enzymatic studies of DNA hairpins containing mismatches in the EcoRI recognition site. Biochem Cell Biol (1998) 0.77
Studies on the heterogeneity of the 5' ends of the protamine mRNAs from rainbow trout testis. Biosci Rep (1981) 0.77
Optimal strategies for the chemical and enzymatic synthesis of bihelical deoxyribonucleic acids. J Am Chem Soc (1975) 0.77
Spectroscopic and thermodynamic studies of DNA duplexes containing alpha-anomeric C, A, and G nucleotides and polarity reversals: coexistence of localized parallel and antiparallel DNA. Biochemistry (1997) 0.77