Kinetic solvent isotope effects on the deacylation of specific acyl-papains. Proton inventory studies on the papain-catalysed hydrolyses of specific ester substrates: analysis of possible transition state structures.

Beta-lactamases as fully efficient enzymes. Determination of all the rate constants in the acyl-enzyme mechanism. Biochem J (1990) 1.75

Variation in aspects of cysteine proteinase catalytic mechanism deduced by spectroscopic observation of dithioester intermediates, kinetic analysis and molecular dynamics simulations. Biochem J (2001) 0.91

Studies on the catalytic mechanism of a glutamic peptidase. J Biol Chem (2010) 0.77

Role of a buried acid group in the mechanism of action of chymotrypsin. Nature (1969) 4.36

The reliability of Michaelis constants and maximum velocities estimated by using the integrated Michaelis-Menten equation. Biochem J (1973) 2.34

KINETICS OF PAPAIN-CATALYZED HYDROLYSIS OF ALPHA-N-BENZOYL-L-ARGININE ETHYL ESTER AND ALPHA-N-BENZOYL-L-ARGININAMIDE. J Am Chem Soc (1965) 2.13

Effect of cysteine-25 on the ionization of histidine-159 in papain as determined by proton nuclear magnetic resonance spectroscopy. Evidence for a his-159--Cys-25 ion pair and its possible role in catalysis. Biochemistry (1981) 1.64

Kinetic specificity in papain-catalysed hydrolyses. Biochem J (1971) 1.60

The preparation and properties of trans-cinnamoyl-papain. J Am Chem Soc (1966) 1.53

Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelain- and papain-catalysed hydrolyses. Biochem J (1974) 1.47

Mercaptide-imidazolium ion-pair: the reactive nucleophile in papain catalysis. FEBS Lett (1974) 1.44

Mechanism of action of serine proteases: tetrahedral intermediate and concerted proton transfer. Biochemistry (1976) 1.41

Evidence that binding to the s2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25-histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a hydrophobic side chain. Biochem J (1979) 1.31

Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the papain catalytic mechanism. Biochem J (1978) 1.24

Cryoenzymology of papain: reaction mechanism with an ester substrate. Biochemistry (1978) 1.16

Inhibition of bovine factor IXa and factor Xabeta by antithrombin III. Biochemistry (1976) 1.16

Carbon dioxide hydration activity of carbonic anhydrase in mixtures of water and deuterium oxide. Biochemistry (1980) 1.11

Potentiometric determination of ionizations at the active site of papain. Biochemistry (1976) 1.07

The kinetics of the papain-catalyzed hydrolysis of esters of carbobenzoxyglycine. Evidence for an acyl-enzyme intermediate. Biochemistry (1966) 1.01

Kinetics of papain-catalyzed hydrolyses of neutral substrates. Biochemistry (1967) 0.96

Some pertinent aspects of mechanism as determined with small molecules. Annu Rev Biochem (1976) 0.94

Determination of a low pK for histidine-159 in the S-methylthio derivative of papain by proton nuclear magnetic resonance spectroscopy. Biochemistry (1981) 0.90

Evidence for association-activation effects in reactions of papain from studies on its reactivity towards isomeric two-protonic-state reactivity probes. Biochem J (1979) 0.89

Perturbations in the free energy and enthalpy of ionization of histidine-159 at the active site of papain as determined by fluorescence spectroscopy. Biochemistry (1981) 0.87

Identification of the functional ionic groups of papain by pH/rate profile analysis. Eur J Biochem (1978) 0.85

Effects of substituents on the rates of deacylation of substituted benzoyl papains. Role of a carboxylate residue in the catalytic mechanism. Biochemistry (1978) 0.80

Multiple isotope effect probes of glutamate decarboxylase. Biochemistry (1981) 0.78

Half-time analysis of the integrated Michaelis equation. Simulation and use of the half-time plot and its direct linear variant in the analysis of some alpha-chymotrypsin, papain- and fumarase-catalysed reactions. Biochem J (1982) 2.15

A critical analysis of the use of radiation inactivation to measure the mass of protein. Radiat Res (1995) 1.52

Kinetics of the hydrolysis of N-benzoyl-L-serine methyl ester catalysed by bromelain and by papain. Analysis of modifier mechanisms by lattice nomography, computational methods of parameter evaluation for substrate-activated catalyses and consequences of postulated non-productive binding in bromelain- and papain-catalysed hydrolyses. Biochem J (1974) 1.47

The preparation and some properties of bromelain covalently attached to O-(carboxymethyl)-cellulose. Eur J Biochem (1968) 1.22

Consequences of molecular recognition in the S1-S2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P1-P2 amide bond into a two-protonic-state reactivity probe. Biochem J (1988) 1.22

Hydrogen-bonding in enzyme catalysis. Fourier-transform infrared detection of ground-state electronic strain in acyl-chymotrypsins and analysis of the kinetic consequences. Biochem J (1990) 1.11

The nature of the perturbation of the michaelis constant of the bromelain-catalysed hydrolysis of alpha-N-benzoyl-L-arginine ethyl ester consequent upon attachment of bromelain to O-(carboxymethyl)-cellulose. Eur J Biochem (1968) 1.05

Conserved residues in the mechanism of the E. coli Class II FBP-aldolase. J Mol Biol (1999) 1.04

Infra-red and Raman spectroscopic studies of enzyme structure and function. Biochem J (1986) 1.02

Fourier-transform infra-red studies of the alkaline isomerization of mitochondrial cytochrome c and the ionization of carboxylic acids. Biochem J (1989) 0.96

Kinetics and mechanism of DNA repair. Preparation, purification and some properties of caged dideoxynucleoside triphosphates. Biochem J (1990) 0.91

The interplay of electrostatic and binding interactions determining active centre chemistry and catalytic activity in actinidin and papain. Biochem J (1989) 0.90

Effect of specificity on ligand conformation in acyl-chymotrypsins. Biochem J (1994) 0.90

Analysis and elimination of protein perturbation in infrared difference spectra of acyl-chymotrypsin ester carbonyl groups by using 13C isotopic substitution. Biochem J (1992) 0.89

Resonance Raman and Fourier transform infrared spectroscopic studies of the acyl carbonyl group in [3-(5-methyl-2-thienyl)acryloyl]chymotrypsin: evidence for artifacts in the spectra obtained by both techniques. Biochemistry (1991) 0.88

Electroporation-induced damage in mammalian cell DNA. Biochim Biophys Acta (1995) 0.86

The kinetics and mechanism of repair of UV induced DNA damage in mammalian cells. The use of 'caged' nucleotides and electroporation to study short time course events in DNA repair. Nucleic Acids Res (1994) 0.86

Hydrogen bonding and protein perturbation in beta-lactam acyl-enzymes of Streptococcus pneumoniae penicillin-binding protein PBP2x. Biochem J (1999) 0.86

Fourier transform infrared spectroscopy of (13C=O) trans-cinnamoyl- and (13C=O) hydrocinnamoyl-a-chymotrypsins. Biochem Soc Trans (1991) 0.85

Multiple conformations of the acylenzyme formed in the hydrolysis of methicillin by Citrobacter freundii beta-lactamase: a time-resolved FTIR spectroscopic study. Biochemistry (1999) 0.83

The synthesis of co-polymers with pendant functional groups arranged in a predetermined geometry as enzyme models [proceedings]. Biochem Soc Trans (1978) 0.82

Kinetic aspects of ATP amplification reactions. Anal Biochem (1998) 0.82

Novel natural product 5,5-trans-lactone inhibitors of human alpha-thrombin: mechanism of action and structural studies. Biochemistry (1998) 0.82

Kinetics and mechanism of DNA repair. Evaluation of caged compounds for use in studies of u.v.-induced DNA repair. Biochem J (1990) 0.80

Benzyloxycarbonylphenylalanylcitrulline p-nitroanilide as a substrate for papain and other plant cysteine proteinases. Biochem J (1984) 0.80

A stopped-flow apparatus for infrared spectroscopy of aqueous solutions. Biochem J (1995) 0.80

Gap junction communication dynamics and bystander effects from ultrasoft X-rays. Br J Cancer (2004) 0.80

The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester. Biochem J (1974) 0.79

Secondary structure of the C-terminal DNA-binding domain of the transcriptional activator NifA from Klebsiella pneumoniae: spectroscopic analyses. Arch Biochem Biophys (1999) 0.79

Analysis of hydrogen bonding in enzyme-substrate complexes of chloramphenicol acetyltransferase by infrared spectroscopy and site-directed mutagenesis. Biochemistry (1994) 0.78

The binding of amide substrate analogues to phospholipase A2. Studies by 13C-nuclear-magnetic-resonance and infrared spectroscopy. Biochem J (1992) 0.77

Resonance Raman spectroscopy of 4-thiothymidine and oligodeoxynucleotides containing this base both free in solution and bound to the restriction endonuclease EcoRV. Biochemistry (1996) 0.77

An ultraviolet resonance Raman study of dehydrogenase enzymes and their interactions with coenzymes and substrates. Biochemistry (1989) 0.77

Some recent advances in enzyme kinetics. Biochem Soc Trans (1983) 0.75

Repair of soft X-ray damage to mammalian cell DNA. Biochem Soc Trans (1990) 0.75

Structural and mechanistic studies on citrate synthase by nuclear magnetic resonance and Fourier transform infra-red spectroscopies. Biochem Soc Trans (1990) 0.75

Stimulation of DNA synthesis by 340nm/351nm UV laser irradiation. Biochem Soc Trans (1991) 0.75

Light amplification by a coupled biological system: ATP, firefly luciferase and recycling of ATP. Biochem Soc Trans (1991) 0.75

Use of caged compounds in studies of the kinetics of DNA repair. Methods Enzymol (1998) 0.75

The kinetics of DNA repair of u.v. damage in mammalian cells. Biochem Soc Trans (1990) 0.75

Novel ligands for the purification of catechol-O-methyltransferase by affinity chromatography. Biochem Pharmacol (1976) 0.75

Modulation of firefly luciferase bioluminescence at bioelectrochemical interfaces. Anal Chem (1998) 0.75