Published in RNA on April 01, 1997
Predicting oligonucleotide affinity to nucleic acid targets. RNA (1999) 2.67
Secondary structure prediction and in vitro accessibility of mRNA as tools in the selection of target sites for ribozymes. Nucleic Acids Res (2000) 1.44
Mapping of RNA accessible sites by extension of random oligonucleotide libraries with reverse transcriptase. RNA (2001) 1.42
RNA accessibility prediction: a theoretical approach is consistent with experimental studies in cell extracts. Nucleic Acids Res (2000) 1.27
A rapid in vitro method for obtaining RNA accessibility patterns for complementary DNA probes: correlation with an intracellular pattern and known RNA structures. Nucleic Acids Res (1997) 1.26
Ribozyme-based gene-inactivation systems require a fine comprehension of their substrate specificities; the case of delta ribozyme. Curr Med Chem (2003) 1.10
Combinatorial screening and intracellular antiviral activity of hairpin ribozymes directed against hepatitis B virus. J Virol (1999) 1.05
Development and comparison of procedures for the selection of delta ribozyme cleavage sites within the hepatitis B virus. Nucleic Acids Res (2002) 0.98
Sequence specificity of the hammerhead ribozyme revisited; the NHH rule. Nucleic Acids Res (1998) 0.95
The effect of structure in a long target RNA on ribozyme cleavage efficiency. Nucleic Acids Res (1997) 0.93
In vitro selection of a purine nucleotide-specific hammerheadlike ribozyme. Proc Natl Acad Sci U S A (1998) 0.93
Secondary structure and hybridization accessibility of hepatitis C virus 3'-terminal sequences. J Virol (2002) 0.92
Mapping of accessible sites for oligonucleotide hybridization on hepatitis delta virus ribozymes. Nucleic Acids Res (2000) 0.91
HIV-1 LTR as a target for synthetic ribozyme-mediated inhibition of gene expression: site selection and inhibition in cell culture. Nucleic Acids Res (2000) 0.88
A selection system for identifying accessible sites in target RNAs. RNA (2001) 0.87
A genomic selection strategy to identify accessible and dimerization blocking targets in the 5'-UTR of HIV-1 RNA. Nucleic Acids Res (2004) 0.85
Identifying ribozyme-accessible sites using NUH triplet-targeting gapmers. Nucleic Acids Res (2001) 0.81
Development of lead hammerhead ribozyme candidates against human rod opsin mRNA for retinal degeneration therapy. Exp Eye Res (2008) 0.80
The impact of mRNA structure on guide RNA targeting in kinetoplastid RNA editing. PLoS One (2010) 0.78
Improvement of RNA secondary structure prediction using RNase H cleavage and randomized oligonucleotides. Nucleic Acids Res (2009) 0.77
Variables and strategies in development of therapeutic post-transcriptional gene silencing agents. J Ophthalmol (2011) 0.76
The rapid generation of oligonucleotide-directed mutations at high frequency using phosphorothioate-modified DNA. Nucleic Acids Res (1985) 12.11
Amplification, enhanced expression and possible rearrangement of EGF receptor gene in primary human brain tumours of glial origin. Nature (1985) 8.23
Nucleoside phosphorothioates. Annu Rev Biochem (1985) 7.55
Incorporation of phosphorothioate groups into fd and phi X174 DNA. Biochemistry (1977) 6.10
Inhibition of restriction endonuclease Nci I cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis. Nucleic Acids Res (1986) 5.90
Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med (2000) 5.83
Deoxynucleoside [1-thio]triphosphates prevent proofreading during in vitro DNA synthesis. Proc Natl Acad Sci U S A (1981) 4.14
The use of phosphorothioate-modified DNA in restriction enzyme reactions to prepare nicked DNA. Nucleic Acids Res (1985) 3.65
Acute stress facilitates long-lasting changes in cholinergic gene expression. Nature (1998) 3.46
5'-3' exonucleases in phosphorothioate-based oligonucleotide-directed mutagenesis. Nucleic Acids Res (1988) 3.41
Pyridostigmine brain penetration under stress enhances neuronal excitability and induces early immediate transcriptional response. Nat Med (1996) 3.30
Kinetic characterization of ribonuclease-resistant 2'-modified hammerhead ribozymes. Science (1991) 3.18
Guanosine 5'-O-(2-thiodiphosphate). An inhibitor of adenylate cyclase stimulation by guanine nucleotides and fluoride ions. J Biol Chem (1979) 3.12
Synthesis and characterization of an octanucleotide containing the EcoRI recognition sequence with a phosphorothioate group at the cleavage site. Biochemistry (1984) 3.03
DNA and RNA sequence determination based on phosphorothioate chemistry. Science (1988) 2.95
Two interferon mRNAs in human fibroblasts: in vitro translation and Escherichia coli cloning studies. Proc Natl Acad Sci U S A (1980) 2.88
Acetylcholinesterase--new roles for an old actor. Nat Rev Neurosci (2001) 2.87
Expression of epidermal growth factor receptors in human brain tumors. Cancer Res (1984) 2.83
Numbering system for the hammerhead. Nucleic Acids Res (1992) 2.74
The magnesium ion-dependent adenosine triphosphatase of myosin. Two-step processes of adenosine triphosphate association and adenosine diphosphate dissociation. Biochem J (1974) 2.57
A study of the mechanism of DNA polymerase I from Escherichia coli with diastereomeric phosphorothioate analogs of deoxyadenosine triphosphate. J Biol Chem (1979) 2.37
Direct sequencing of polymerase chain reaction amplified DNA fragments through the incorporation of deoxynucleoside alpha-thiotriphosphates. Nucleic Acids Res (1988) 2.28
Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing. Nucleic Acids Res (1989) 2.21
Synthesis and configurational analysis of a dinucleoside phosphate isotopically chiral at phosphorus. Stereochemical course of Penicillium citrum nuclease P1 reaction. Biochemistry (1983) 2.18
Properties of overexpressed phage T5 D15 exonuclease. Similarities with Escherichia coli DNA polymerase I 5'-3' exonuclease. J Biol Chem (1990) 2.17
Role of the polyadenylate segment in the translation of globin messenger RNA in Xenopus oocytes. Proc Natl Acad Sci U S A (1974) 2.05
Interaction of hydrolysis-resistant analogs of cyclic GMP with the phosphodiesterase and light-sensitive channel of retinal rod outer segments. Proc Natl Acad Sci U S A (1985) 2.04
Synthesis and properties of diastereoisomers of adenosine 5'-(O-1-thiotriphosphate) and adenosine 5'-(O-2-thiotriphosphate). Biochemistry (1976) 2.02
Incorporation of terminal phosphorothioates into oligonucleotides. Nucleic Acids Res (1998) 2.00
The structure, function and application of the hammerhead ribozyme. Eur J Biochem (1997) 1.94
Left-handed DNA: from synthetic polymers to chromosomes. J Biomol Struct Dyn (1983) 1.90
[Synthesis of oligodesoxynucleotides with phosphoric acid triester]. Chem Ber (1969) 1.89
Genetic predisposition to adverse consequences of anti-cholinesterases in 'atypical' BCHE carriers. Nat Med (1995) 1.87
Protection of oligonucleotide primers against degradation by DNA polymerase I. Biochemistry (1987) 1.87
Modified oligonucleotides: synthesis and strategy for users. Annu Rev Biochem (1998) 1.85
Absolute configuration of the diastereomers of adenosine 5'-O-(1-thiotriphosphate): consequences for the stereochemistry of polymerization by DNA-dependent RNA polymerase from Escherichia coli. Proc Natl Acad Sci U S A (1978) 1.82
Absolute stereochemistry of the second step of ribonuclease action. Nature (1970) 1.80
Two autolytic processing reactions of a satellite RNA proceed with inversion of configuration. Nucleic Acids Res (1990) 1.80
Fluorescent labelling of tRNA and oligodeoxynucleotides using T4 RNA ligase. Nucleic Acids Res (1984) 1.77
Regional analysis of femorotibial cartilage loss in a subsample from the Osteoarthritis Initiative progression subcohort. Osteoarthritis Cartilage (2008) 1.76
Magnetic resonance imaging-based assessment of cartilage loss in severe osteoarthritis: accuracy, precision, and diagnostic value. Arthritis Rheum (2001) 1.76
Quantitative determination of joint incongruity and pressure distribution during simulated gait and cartilage thickness in the human hip joint. J Orthop Res (1999) 1.75
Nucleoside phosphorothioates. J Am Chem Soc (1970) 1.71
A three-dimensional model for the hammerhead ribozyme based on fluorescence measurements. Science (1994) 1.71
Geometry of the first step in the action of ribonuclease-A (in-line geometry-uridine2',3'-cyclic thiophosphate- 31 P NMR). Proc Natl Acad Sci U S A (1972) 1.71
Hammerhead ribozymes: importance of stem-loop II for activity. Proc Natl Acad Sci U S A (1993) 1.69
Interaction of Escherichia coli tRNA(Ser) with its cognate aminoacyl-tRNA synthetase as determined by footprinting with phosphorothioate-containing tRNA transcripts. Proc Natl Acad Sci U S A (1991) 1.69
MRI protocols for whole-organ assessment of the knee in osteoarthritis. Osteoarthritis Cartilage (2006) 1.68
Stereochemistry of polymerization by DNA-dependent RNA-polymerase from Escherichia coli: an investigation with a diastereomeric ATP-analogue. Proc Natl Acad Sci U S A (1976) 1.65
Diastereomers of 5'-O-adenosyl 3'-O-uridyl phosphorothioate: chemical synthesis and enzymatic properties. Biochemistry (1979) 1.65
Phosphorothioates in molecular biology. Trends Biochem Sci (1989) 1.62
Importance of exocyclic base functional groups of central core guanosines for hammerhead ribozyme activity. Biochemistry (1993) 1.62
Cleavage of phosphorothioate-substituted DNA by restriction endonucleases. J Biol Chem (1984) 1.61
Globin mRNA species containing poly(A) segments of different lengths. Their functional stability in Xenopus oocytes. Eur J Biochem (1976) 1.61
Isolation and characterization of full-length cDNA clones coding for cholinesterase from fetal human tissues. Proc Natl Acad Sci U S A (1987) 1.61
Oligonucleotide duplexes containing 2'-amino-2'-deoxycytidines: thermal stability and chemical reactivity. Nucleic Acids Res (1994) 1.60
Age-related changes in the morphology and deformational behavior of knee joint cartilage. Arthritis Rheum (2001) 1.58
Stereochemistry of hydrolysis by snake venom phosphodiesterase. J Biol Chem (1979) 1.58
Treatment of human myasthenia gravis with oral antisense suppression of acetylcholinesterase. Neurology (2007) 1.51
X inactivation in mammalian testis is correlated with inactive X-specific transcription. Nat Genet (1992) 1.51
Absence of polyadenylate segment in globin messenger RNA accelerates its degradation in Xenopus oocytes. Proc Natl Acad Sci U S A (1975) 1.51
Dynamics of the RNA hairpin GNRA tetraloop. Biochemistry (2000) 1.47
Synthesis of d(GC) and d(CG) octamers containing alternating phosphorothioate linkages: effect of the phosphorothioate group on the B-Z transition. Biochemistry (1985) 1.46
Hammerhead ribozyme-mediated cleavage of the long terminal repeat RNA of human immunodeficiency virus type 1. J Biol Chem (1992) 1.45
The importance of being ribose at the cleavage site in the Tetrahymena ribozyme reaction. Biochemistry (1993) 1.45
In vitro translation of polyadenylic acid-free rabbit globin messenger RNA. J Mol Biol (1974) 1.45
Investigation of enzyme mechanisms with nucleoside phosphorothioates. Angew Chem Int Ed Engl (1975) 1.44
A study of the mechanism of T4 DNA polymerase with diastereomeric phosphorothioate analogues of deoxyadenosine triphosphate. J Biol Chem (1982) 1.44
Contributions of 2'-hydroxyl groups of the RNA substrate to binding and catalysis by the Tetrahymena ribozyme. An energetic picture of an active site composed of RNA. Biochemistry (1993) 1.44
Chiral phosphorothioate analogues of B-DNA. The crystal structure of Rp-d[Gp(S)CpGp(S)CpGp(S)C]. J Mol Biol (1986) 1.43
Filter disc supported oligonucleotide synthesis by the phosphite method. Nucleic Acids Res (1984) 1.43
2'-Fluoro- and 2'-amino-2'-deoxynucleoside 5'-triphosphates as substrates for T7 RNA polymerase. Biochemistry (1992) 1.41
Xenopus oocyte microinjection: from gene to protein. Methods Enzymol (1992) 1.41
Function of specific 2'-hydroxyl groups of guanosines in a hammerhead ribozyme probed by 2' modifications. Proc Natl Acad Sci U S A (1992) 1.41
Stereochemical course of DNA hydrolysis by nuclease S1. J Biol Chem (1983) 1.41
The osteoporotic vertebral structure is well adapted to the loads of daily life, but not to infrequent "error" loads. Bone (2004) 1.41
In situ measurement of articular cartilage deformation in intact femoropatellar joints under static loading. J Biomech (1999) 1.40
Definition of osteoarthritis on MRI: results of a Delphi exercise. Osteoarthritis Cartilage (2011) 1.40
Readenylation of polyadenylate-free globin messenger RNA restores its stability in vivo. Eur J Biochem (1975) 1.40
Mg(2+)-dependent conformational changes in the hammerhead ribozyme. Biochemistry (1996) 1.40
Change in regional cartilage morphology and joint space width in osteoarthritis participants versus healthy controls: a multicentre study using 3.0 Tesla MRI and Lyon-Schuss radiography. Ann Rheum Dis (2010) 1.38
Generation of left-handed Z-DNA in solution and visualization in polytene chromosomes by immunofluorescence. Cold Spring Harb Symp Quant Biol (1983) 1.36
Gender differences in knee joint cartilage thickness, volume and articular surface areas: assessment with quantitative three-dimensional MR imaging. Skeletal Radiol (2001) 1.35
Structural roles of acetylcholinesterase variants in biology and pathology. Eur J Biochem (1999) 1.35
Translational activity and functional stability of human fibroblast beta 1 and beta 2 interferon mRNAs lacking 3'-terminal RNA sequences. Proc Natl Acad Sci U S A (1981) 1.35
Determination of 3D cartilage thickness data from MR imaging: computational method and reproducibility in the living. Magn Reson Med (1999) 1.34
The stereochemical course of the restriction endonuclease EcoRI-catalyzed reaction. J Biol Chem (1984) 1.33
Interobserver reproducibility of quantitative cartilage measurements: comparison of B-spline snakes and manual segmentation. Magn Reson Imaging (1999) 1.33
Acetylcholinesterase enhances neurite growth and synapse development through alternative contributions of its hydrolytic capacity, core protein, and variable C termini. J Neurosci (1998) 1.32
Mechanism of ribose 2'-group discrimination by an RNA polymerase. Biochemistry (1997) 1.32
Sustained cholinesterase inhibition in AD patients receiving rivastigmine for 12 months. Neurology (2002) 1.30
Crystal and molecular structure of adenosine 5'-O-phosphorothioate O-p-nitrophenyl ester (Sp diastereomer). Substrate stereospecificity of snake venom phosphodiesterase. Eur J Biochem (1979) 1.29
Stereochemistry of the guanyl nucleotide binding site of transducin probed by phosphorothioate analogues of GTP and GDP. Biochemistry (1985) 1.29
Polynucleotides containing 2'-amino-2'-deoxyribose and 2'-azido-2'-deoxyribose. Biochemistry (1973) 1.27
Premorbid knee osteoarthritis is not characterised by diffuse thinness: the Framingham Osteoarthritis Study. Ann Rheum Dis (2008) 1.27
Left-handed helical conformation of poly[d(A-m5C).d(G-T)]. Nature (1983) 1.27
Amplification and overexpression of the EGF receptor gene in primary human glioblastomas. J Cell Sci Suppl (1985) 1.27
Knee cartilage of spinal cord-injured patients displays progressive thinning in the absence of normal joint loading and movement. Arthritis Rheum (2002) 1.25