Design and testing of beta-actin primers for RT-PCR that do not co-amplify processed pseudogenes.

PubWeight™: 1.54‹?› | Rank: Top 4%

🔗 View Article (PMID 9298216)

Published in Biotechniques on September 01, 1997

Authors

T Raff1, M van der Giet, D Endemann, T Wiederholt, M Paul

Author Affiliations

1: Universitätsklinikum Benjamin Franklin Free University, Berlin, Germany.

Articles citing this

Telomere dysfunction triggers extensive DNA fragmentation and evolution of complex chromosome abnormalities in human malignant tumors. Proc Natl Acad Sci U S A (2001) 2.91

Requirement for the lpA1 lysophosphatidic acid receptor gene in normal suckling behavior. Proc Natl Acad Sci U S A (2000) 2.60

Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis. Am J Pathol (1999) 2.59

GeneScan reverse transcription-PCR assay for detection of six common respiratory viruses in young children hospitalized with acute respiratory illness. J Clin Microbiol (2003) 1.83

Human cathelicidin (LL-37), a multifunctional peptide, is expressed by ocular surface epithelia and has potent antibacterial and antiviral activity. Curr Eye Res (2005) 1.58

HPP1: a transmembrane protein-encoding gene commonly methylated in colorectal polyps and cancers. Proc Natl Acad Sci U S A (2001) 1.41

Macaques with rapid disease progression and simian immunodeficiency virus encephalitis have a unique cytokine profile in peripheral lymphoid tissues. J Virol (2001) 1.38

HLA-G allelic variants are associated with differences in the HLA-G mRNA isoform profile and HLA-G mRNA levels. Immunogenetics (2003) 1.38

Enhanced expression of proinflammatory cytokines in the central nervous system is associated with neuroinvasion by simian immunodeficiency virus and the development of encephalitis. J Virol (2002) 1.27

Endothelin-1 from prostate cancer cells is enhanced by bone contact which blocks osteoclastic bone resorption. Br J Cancer (2000) 1.09

Impact of a school-based influenza immunization program on disease burden: comparison of two Tennessee counties. Vaccine (2009) 1.07

Adrenergic signalling between rat taste receptor cells. J Physiol (2002) 1.06

Ocular surface expression and in vitro activity of antimicrobial peptides. Curr Eye Res (2007) 1.06

Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Mamm Genome (2007) 1.04

Centrosomal abnormalities, multipolar mitoses, and chromosomal instability in head and neck tumours with dysfunctional telomeres. Br J Cancer (2002) 1.03

High rate of viral identification and coinfections in infants with acute bronchiolitis. Clinics (Sao Paulo) (2010) 1.03

Microarray analysis of relative gene expression stability for selection of internal reference genes in the rhesus macaque brain. BMC Mol Biol (2010) 1.02

No direct effect of the -521 C/T polymorphism in the human dopamine D4 receptor gene promoter on transcriptional activity. BMC Mol Biol (2006) 0.98

PDZK1 is a novel factor in breast cancer that is indirectly regulated by estrogen through IGF-1R and promotes estrogen-mediated growth. Mol Med (2013) 0.95

Ochratoxin A-induced mutagenesis in mammalian cells is consistent with the production of oxidative stress. Chem Res Toxicol (2007) 0.94

Double inhibition of XIAP and Bcl-2 axis is beneficial for retrieving sensitivity of renal cell cancer to apoptosis. Br J Cancer (2008) 0.92

High levels of Lymphotoxin-Beta (LT-Beta) gene expression in rheumatoid arthritis synovium: clinical and cytokine correlations. Rheumatol Int (2008) 0.91

Improved methods for extracting RNA from exfoliated human colonocytes in stool and RT-PCR analysis. Dig Dis Sci (2005) 0.85

Allele-specific impairment of GJB2 expression by GJB6 deletion del(GJB6-D13S1854). PLoS One (2011) 0.82

Selective cancer-germline gene expression in pediatric brain tumors. J Neurooncol (2008) 0.78

Enhanced expression of IFN-gamma mRNA in CD4(+)or CD8(+)tumour-infiltrating lymphocytes compared to peripheral lymphocytes in patients with renal cell cancer. Br J Cancer (2000) 0.77

Ultraviolet-A radiation induces changes in cyclin G gene expression in mouse melanoma B16-F1 cells. Cancer Cell Int (2007) 0.76

Expression pattern of the urokinase-plasminogen activator system in rat DS-sarcoma: role of oxygenation status and tumour size. Br J Cancer (2002) 0.75

Ectromelia virus accumulates less double-stranded RNA compared to vaccinia virus in BS-C-1 cells. Virology (2017) 0.75

Articles by these authors

(truncated to the top 100)

Resection of colorectal liver metastases. World J Surg (1995) 7.73

Prevention of radiographic-contrast-agent-induced reductions in renal function by acetylcysteine. N Engl J Med (2000) 4.67

Identification of p53 gene mutations in bladder cancers and urine samples. Science (1991) 3.68

Serological studies in cholera. I. Vibrio agglutinin response of cholera patients determined by a microtechnique. Bull World Health Organ (1968) 3.47

Morphology, physiology, and molecular biology of renin secretion. Physiol Rev (1990) 2.71

Accidental tenfold overdose of propofol in a 6-month old infant undergoing elective craniosynostosis repair. Anaesthesia (2004) 2.64

The angiotensin AT2-receptor mediates inhibition of cell proliferation in coronary endothelial cells. J Clin Invest (1995) 2.35

A new oscillometric method for pulse wave analysis: comparison with a common tonometric method. J Hum Hypertens (2010) 2.19

Monotherapy versus beta-lactam-aminoglycoside combination treatment for gram-negative bacteremia: a prospective, observational study. Antimicrob Agents Chemother (1997) 2.00

The wide spectrum of multidrug resistance 3 deficiency: from neonatal cholestasis to cirrhosis of adulthood. Gastroenterology (2001) 1.99

Epidemiology and clinical features of vivax malaria imported to Europe: sentinel surveillance data from TropNetEurop. Malar J (2004) 1.90

Specific protein synthesis in cellular differentiation. Production of eggshell proteins by silkmoth follicular cells. J Cell Biol (1972) 1.82

Demonstration of renin mRNA, angiotensinogen mRNA, and angiotensin converting enzyme mRNA expression in the human eye: evidence for an intraocular renin-angiotensin system. Br J Ophthalmol (1996) 1.74

Transition to adult services for children and young people with palliative care needs: a systematic review. Arch Dis Child (2009) 1.73

Snake bites by the Papuan taipan (Oxyuranus scutellatus canni): paralysis, hemostatic and electrocardiographic abnormalities, and effects of antivenom. Am J Trop Med Hyg (1995) 1.69

Prophylaxis regimens for GVHD: systematic review and meta-analysis. Bone Marrow Transplant (2008) 1.63

Relationship between environmental tobacco smoke exposure and carcinogen-hemoglobin adduct levels in nonsmokers. J Natl Cancer Inst (1993) 1.61

Upregulation of the vascular NAD(P)H-oxidase isoforms Nox1 and Nox4 by the renin-angiotensin system in vitro and in vivo. Free Radic Biol Med (2001) 1.60

A probasin-large T antigen transgenic mouse line develops prostate adenocarcinoma and neuroendocrine carcinoma with metastatic potential. Cancer Res (2001) 1.59

Oral versus intravenous antibiotic treatment for febrile neutropenia in cancer patients. Cochrane Database Syst Rev (2004) 1.59

The eggshell of insects: differentiation-specific proteins and the control of their synthesis and accumulation during development. Results Probl Cell Differ (1977) 1.56

Antibiotic prophylaxis for bacterial infections in afebrile neutropenic patients following chemotherapy. Cochrane Database Syst Rev (2005) 1.53

A polymorphism in the endothelin-A receptor gene predicts survival in patients with idiopathic dilated cardiomyopathy. Eur Heart J (2001) 1.53

Diadenosine phosphates and the physiological control of blood pressure. Nature (1994) 1.53

Mesangial IgA deposition in minimal change nephrotic syndrome: coincidence of different entities or variant of minimal change disease? Clin Nephrol (2006) 1.51

In utero exposure to low doses of bisphenol A lead to long-term deleterious effects in the vagina. Neoplasia (2002) 1.48

Modelling in infectious diseases: between haphazard and hazard. Clin Microbiol Infect (2013) 1.47

Tissue-specific activation of cardiac angiotensin converting enzyme in experimental heart failure. Circ Res (1991) 1.43

To resuscitate or not. Med J Aust (1990) 1.41

Fertilization-associated light-scattering changes in eggs of the sea urchin Strongylocentrotus purpuratus. Exp Cell Res (1971) 1.41

Colistin: new lessons on an old antibiotic. Clin Microbiol Infect (2012) 1.40

Optic neuritis complicating west nile virus meningitis in a young adult. Infection (2003) 1.39

Specific protein synthesis in cellular differentiation. II. The program of protein synthetic changes during chorion formation by slikmoth follicles, and its implementation in organ culture. Dev Biol (1975) 1.38

Open letter to the Greenock telegraph, Greenock, Scotland. Int J Occup Environ Health (1999) 1.37

Lessons from rat models of hypertension: from Goldblatt to genetic engineering. Cardiovasc Res (1998) 1.37

Gap junction protein connexin40 is preferentially expressed in vascular endothelium and conductive bundles of rat myocardium and is increased under hypertensive conditions. Circ Res (1993) 1.35

Patch testing in severe cutaneous adverse drug reactions, including Stevens-Johnson syndrome and toxic epidermal necrolysis. Contact Dermatitis (1996) 1.34

Expression and function of endothelial Ca(2+)-activated K(+) channels in human mesenteric artery: A single-cell reverse transcriptase-polymerase chain reaction and electrophysiological study in situ. Circ Res (2000) 1.34

A shorter 146Sm half-life measured and implications for 146Sm-142Nd chronology in the solar system. Science (2012) 1.31

In situ observation of living pericytes in rat retinal capillaries. Microvasc Res (1998) 1.30

The significance of Acinetobacter baumannii bacteraemia compared with Klebsiella pneumoniae bacteraemia: risk factors and outcomes. J Hosp Infect (2006) 1.27

Regulation of Raf by Akt controls growth and differentiation in vascular smooth muscle cells. J Biol Chem (2001) 1.26

Health, equity, and reproductive risks in the workplace. J Public Health Policy (1990) 1.24

Duration of antibacterial treatment for uncomplicated urinary tract infection in women. Cochrane Database Syst Rev (2005) 1.23

Improving empirical antibiotic treatment: prospective, nonintervention testing of a decision support system. J Intern Med (1997) 1.21

Evidence that the medial amygdala projects to the anterior/ventromedial hypothalamic nuclei to inhibit maternal behavior in rats. Neuroscience (2001) 1.19

Endothelial K(+) channel lacks the Ca(2+) sensitivity-regulating beta subunit. FASEB J (2000) 1.17

Species specificity of renin kinetics in transgenic rats harboring the human renin and angiotensinogen genes. Proc Natl Acad Sci U S A (1992) 1.16

Gut bacterial microbiota and obesity. Clin Microbiol Infect (2013) 1.15

Cerebrospinal fluid neopterin in human immunodeficiency virus type 1 infection. Ann Neurol (1990) 1.14

Impact of health education on knowledge and prevention behavior for congenital toxoplasmosis: the experience in Poznań, Poland. Health Educ Res (2001) 1.14

A comparative study of eggshell proteins in lepidoptera. J Supramol Struct (1972) 1.13

Role of AT2 receptors in angiotensin II-stimulated contraction of small mesenteric arteries in young SHR. Hypertension (1999) 1.13

Sodium stibogluconate (Pentostam) potentiates oxidant production in murine visceral leishmaniasis and in human blood. Antimicrob Agents Chemother (2000) 1.09

Negative control elements and cAMP responsive sequences in the tissue-specific expression of mouse renin genes. Proc Natl Acad Sci U S A (1989) 1.09

Evaluation of the bead enzyme-linked immunosorbent assay for detection of cholera toxin directly from stool specimens. J Clin Microbiol (1992) 1.09

NOX1, 2, 4, 5: counting out oxidative stress. Br J Pharmacol (2011) 1.08

*NO and oxyradical metabolism in new cell lines of rat brain capillary endothelial cells forming the blood-brain barrier. Microvasc Res (2001) 1.08

Physiological characterization of the hypertensive transgenic rat TGR(mREN2)27. Am J Physiol (1996) 1.07

Surgical target selection in cerebral glioma surgery: linking methionine (MET) PET image fusion and neuronavigation. Minim Invasive Neurosurg (2007) 1.07

Prophylaxis for Pneumocystis pneumonia (PCP) in non-HIV immunocompromised patients. Cochrane Database Syst Rev (2007) 1.06

RE: Spontaneous abortion in the British semiconductor industry. Am J Ind Med (1999) 1.06

Cerebrospinal fluid beta 2 microglobulin in patients infected with human immunodeficiency virus. Neurology (1989) 1.05

Validation of connective tissue growth factor (CTGF/CCN2) and its gene polymorphisms as noninvasive biomarkers for the assessment of liver fibrosis. J Viral Hepat (2009) 1.05

Plankton food web and its seasonal dynamics in a large monsoonal estuary (Cochin backwaters, India)-significance of mesohaline region. Environ Monit Assess (2015) 1.05

Impaired hyperpolarization in regenerated endothelium after balloon catheter injury. Circ Res (2001) 1.04

Acute blood pressure effects of YC-1-induced activation of soluble guanylyl cyclase in normotensive and hypertensive rats. Br J Pharmacol (2000) 1.04

Virus load as a marker of disease progression in HIV-infected children. AIDS Res Hum Retroviruses (1996) 1.02

Expression and acquisition of the conditioned place preference response to cocaine in rats is blocked by selective inhibitors of the enzyme N-acetylated-alpha-linked-acidic dipeptidase (NAALADASE). Synapse (2001) 1.02

Guidelines for the practical stability studies of anticancer drugs: a European consensus conference. Ann Pharm Fr (2011) 1.01

Comorbidity of autistic spectrum disorders in children with Down syndrome. Dev Med Child Neurol (1999) 1.01

Vaccines for preventing typhoid fever. Cochrane Database Syst Rev (2007) 1.01

First trimester surgical abortion practices: a survey of National Abortion Federation members. Contraception (2001) 1.01

4-Aminobiphenyl hemoglobin adducts in fetuses exposed to the tobacco smoke carcinogen in utero. J Natl Cancer Inst (1991) 1.00

Usefulness of Toxoplasma gondii recombinant antigens (GRA1, GRA7 and SAG1) in an immunoglobulin G avidity test for the serodiagnosis of toxoplasmosis. Parasitol Res (2006) 1.00

Systematic review of randomized controlled trials of atypical antipsychotics and selective serotonin reuptake inhibitors for behavioural problems associated with pervasive developmental disorders. J Psychopharmacol (2005) 1.00

Oxidative stress increases endothelin-1 synthesis in human coronary artery smooth muscle cells. J Cardiovasc Pharmacol (2001) 0.99

Morphogenesis of silkmoth chorion: initial framework formation and its relation to synthesis of specific proteins. Dev Biol (1982) 0.98

Antibiotic prophylaxis of bacterial infections in cirrhotic inpatients: a meta-analysis of randomized controlled trials. Scand J Gastroenterol (2003) 0.98

Identification of a negative regulatory element involved in tissue-specific expression of mouse renin genes. Proc Natl Acad Sci U S A (1992) 0.97

Induction of three-dimensional assembly and increase in apoptosis of human endothelial cells by simulated microgravity: impact of vascular endothelial growth factor. Apoptosis (2006) 0.97

Clonal structure, extended-spectrum β-lactamases, and acquired AmpC-type cephalosporinases of Escherichia coli populations colonizing patients in rehabilitation centers in four countries. Antimicrob Agents Chemother (2012) 0.97

Shigellosis in Calcutta during 1990-1992: antibiotic susceptibility pattern and clinical features. J Diarrhoeal Dis Res (1994) 0.97

Comparative population analysis of Klebsiella pneumoniae strains with extended-spectrum β-lactamases colonizing patients in rehabilitation centers in four countries. Antimicrob Agents Chemother (2013) 0.97

Propofol attenuates responses of the auditory cortex to acoustic stimulation in a dose-dependent manner: a FMRI study. Acta Anaesthesiol Scand (2005) 0.97

Prevention of recurrent hepatitis B virus infection after liver transplantation: hepatitis B immunoglobulin, antiviral drugs, or both? Systematic review and meta-analysis. Transpl Infect Dis (2009) 0.96

Phosphorylation of vasodilator-stimulated phosphoprotein: a consequence of nitric oxide- and cGMP-mediated signal transduction in brain capillary endothelial cells and astrocytes. Brain Res Mol Brain Res (1999) 0.96

Perforated peptic ulcer in pregnancy with survival of mother and child: case report and review of the literature. Can J Surg (1976) 0.96

Risk of tuberculosis in close contacts of patients with multidrug resistant tuberculosis: a nationwide cohort. Thorax (2009) 0.95

Type 1 and type 2 cytokine profiles in children exposed to or infected with vertically transmitted human immunodeficiency virus. Clin Diagn Lab Immunol (1996) 0.95

Departmental consumption of antibiotic drugs and subsequent resistance: a quantitative link. J Antimicrob Chemother (2001) 0.95

Tissue renin-angiotensin systems. Their role in cardiovascular disease. Circulation (1993) 0.95

Contractile systolic and diastolic dysfunction in renin-induced hypertensive cardiomyopathy. Hypertension (1997) 0.94

Oxidative stress increases synthesis of big endothelin-1 by activation of the endothelin-1 promoter. J Mol Cell Cardiol (2000) 0.94

Dynamic aspects of neurohormonal control of cyclic 3',5'-AMP synthesis in brain. Adv Biochem Psychopharmacol (1970) 0.94

Behçet's disease in Israel: the influence of ethnic origin on disease expression and severity. J Rheumatol (2001) 0.94

Drugs for preventing tuberculosis in people at risk of multiple-drug-resistant pulmonary tuberculosis. Cochrane Database Syst Rev (2006) 0.93

Validation of the Stabil-O-Graph blood pressure self-measurement device. J Hum Hypertens (2007) 0.92

Leishmania infantum: lack of parasite resistance to amphotericin B in a clinically resistant visceral leishmaniasis. Antimicrob Agents Chemother (1998) 0.92

Mechanisms of cardiac growth. The role of the renin-angiotensin system. Basic Res Cardiol (1992) 0.92