A substrate-induced change in the stereospecificity of the serine-hydroxymethyltransferase-catalysed exchange of the alpha-protons of amino acids--evidence for a second catalytic site.

The stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the alpha-protons of amino acids. Biochem J (2004) 0.75

A reporter group delivery system with both absolute and selective specificity for thiol groups and an improved fluorescent probe containing the 7-nitrobenzo-2-oxa-1,3-diazole moiety. Biochem J (1975) 3.17

Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe. Biochem J (1976) 2.53

Differences in the interaction of the catalytic groups of the active centres of actinidin and papain. Rapid purification of fully active actinidin by covalent chromatography and characterization of its active centre by use of two-protonic-state reactivity probes. Biochem J (1981) 1.60

The case for assigning a value of approximately 4 to pKa-i of the essential histidine-cysteine interactive systems of papain, bromelain and ficin. FEBS Lett (1975) 1.50

Evidence for a two-state transition in papain that may have no close analogue in ficin. Differences in the disposition of cationic sites and hydrophobic binding areas in the active centres of papain and ficin. Biochem J (1980) 1.39

Evidence that binding to the s2-subsite of papain may be coupled with catalytically relevant structural change involving the cysteine-25-histidine-159 diad. Kinetics of the reaction of papain with a two-protonic-state reactivity probe containing a hydrophobic side chain. Biochem J (1979) 1.31

Differences in the chemical and catalytic characteristics of two crystallographically 'identical' enzyme catalytic sites. Characterization of actinidin and papain by a combination of pH-dependent substrate catalysis kinetics and reactivity probe studies targeted on the catalytic-site thiol group and its immediate microenvironment. Biochem J (1987) 1.30

Rapid type 2 molybdenum(V) electron-paramagnetic resonance signals from xanthine oxidase and the structure of the active centre of the enzyme. Biochem J (1980) 1.24

Mechanism of the reaction of papain with substrate-derived diazomethyl ketones. Implications for the difference in site specificity of halomethyl ketones for serine proteinases and cysteine proteinases and for stereoelectronic requirements in the papain catalytic mechanism. Biochem J (1978) 1.24

13C NMR of cyanylated flavodoxin from Megasphaera elsdenii and of thiocyanate model compounds. Biochemistry (1992) 1.20

Reactivities of neutral and cationic forms of 2,2'-dipyridyl disulphide towards thiolate anions. Detection of differences between the active centres of actinidin, papain and ficin by a three-protonic-state reactivity probe. Biochem J (1979) 1.19

13C NMR study of the ionizations within a trypsin-chloromethyl ketone inhibitor complex. Biochemistry (1985) 1.10

Intramolecular inhibition by enzyme of site-specific modification reactions can mask pKa values characteristic of the reaction pathway: do the side chains of aspartic acid-158 and lysine-156 of papain form an ion-pair? [proceedings]. Biochem Soc Trans (1978) 1.09

A 13C-n.m.r. investigation of the ionizations within an inhibitor--alpha-chymotrypsin complex. Evidence that both alpha-chymotrypsin and trypsin stabilize a hemiketal oxyanion by similar mechanisms. Biochem J (1989) 1.06

The nature of the sulphur atom liberated from xanthine oxidase by cyanide. Evidence from e.p.r. spectroscopy after 35S substitution. Biochem J (1980) 1.06

A 13C-n.m.r. investigation of ionizations within a trypsin-inhibitor complex. Evidence that the pKa of histidine-57 is raised by interaction with the hemiketal oxyanion. Biochem J (1985) 0.97

Evidence that the lack of high catalytic activity of thiolsubtilisin towards specific substrates may be due to an inappropriately located proton-distribution system. Demonstration of highly nucleophilic character of the thiol group of thiolsubtilisin in the catalytically relevant ionization state of the active centre by use of a two-protonic-state reactivity probe. Biochem J (1981) 0.94

Characterization of the electrostatic perturbation of a catalytic site (Cys)-S-/(His)-Im+H ion-pair in one type of serine proteinase architecture by kinetic and computational studies on chemically mutated subtilisin variants. J Mol Biol (1996) 0.93

Coupling of [33S]sulphur to molybdenum(V) in different reduced forms of xanthine oxidase. Biochem J (1981) 0.90

A kinetic method for the study of solvent environments of thiol groups in proteins involving the use of a pair of isomeric reactivity probes and a differential solvent effect. Investigation of the active centre of ficin by using 2,2'- and 4,4'- dipyridyl disulphides as reactivity probes. Biochem J (1980) 0.89

Molybdenum(V) e.p.r. signals obtained from xanthine oxidase on reduction with aldehyde substrates and with 2-amino-4-hydroxy-6-formylpteridine. Biochem J (1981) 0.88

Crystal structure of delta-chymotrypsin bound to a peptidyl chloromethyl ketone inhibitor. Acta Crystallogr D Biol Crystallogr (2000) 0.86

A study of the stabilization of tetrahedral adducts by trypsin and delta-chymotrypsin. Biochem J (1992) 0.85

Cryoenzymology of trypsin. A detailed kinetic study of the trypsin-catalysed hydrolysis of N-alpha-benzyloxycarbonyl-L-lysine p-nitrophenyl ester at low temperatures. Biochem J (1983) 0.85

13C NMR study of the stereospecificity of the thiohemiacetals formed on inhibition of papain by specific enantiomeric aldehydes. Biochemistry (1986) 0.85

13C-n.m.r. of the cyanylated beta-lactoglobulins: evidence that Cys-121 provides the thiol group of beta-lactoglobulins A and B. Biochem J (1994) 0.84

13C-n.m.r. of the cyanylated apoflavodoxin and flavodoxin from Clostridium pasteurianum. Biochem J (1993) 0.83

Factors affecting the stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the pro-2R and pro-2S protons of glycine. Biochem J (1995) 0.82

The effect of different amino acid side chains on the stereospecificity and catalytic efficiency of the tryptophan synthase-catalysed exchange of the alpha-protons of amino acids. Biochem J (1996) 0.82

A proton-magnetic-resonance study of hydrogen-exchange reactions of yeast tryptophan synthase. Biochem J (1991) 0.82

Studying enzyme mechanism by 13C nuclear magnetic resonance. Science (1984) 0.82

A study of the stabilization of the oxyanion of tetrahedral adducts by trypsin, chymotrypsin and subtilisin. Biochem J (1995) 0.82

pH-dependent spectroscopic changes associated with the hydroquinone of FMN in flavodoxins. Biochemistry (1999) 0.81

Substituted pyridines as two-protonic-state reactivity probes, reporter-group delivery vehicles and labelling reagents for the study of thiol enzymes [proceedings]. Biochem Soc Trans (1978) 0.81

The synthesis and characterisation of a glyoxal inhibitor of chymotrypsin. Biochem Soc Trans (1996) 0.81

Determination of the ionization state of the active-site histidine in a subtilisin-(chloromethane inhibitor) derivative by 13C-NMR. Biochem J (1996) 0.81

13C NMR study of how the oxyanion pKa values of subtilisin and chymotrypsin tetrahedral adducts are affected by different amino acid residues binding in enzyme subsites S1-S4. Biochemistry (1999) 0.80

The aspartate aminotransferase-catalysed exchange of the alpha-protons of aspartate and glutamate: the effects of the R386A and R292V mutations on this exchange reaction. Biochim Biophys Acta (1999) 0.79

A study of the tryptophan synthase catalysed H/D exchange of the alpha-protons of amino acids. Biochem Soc Trans (1994) 0.78

Enzymatic synthesis of alpha-deuterated amino acids. Biochem Soc Trans (1996) 0.77

Proof that serine hydroxymethyltransferase retains its specificity for the pro-2S proton of glycine in the absence of tetrahydrofolate. Biochem Soc Trans (1996) 0.77

The pyridoxal-5'-phosphate-dependent catalytic antibody 15A9: its efficiency and stereospecificity in catalysing the exchange of the alpha-protons of glycine. FEBS Lett (1998) 0.77

Chemical synthesis and papain-catalysed hydrolysis of N-alpha-benzyloxycarbonyl-L-lysine p-nitroanilide. Biochem J (1985) 0.76

Cryoenzymology of trypsin. 13C-n.m.r. detection of an acyl-trypsin intermediate in the trypsin-catalysed hydrolysis of a highly specific substrate at subzero temperature. Biochem J (1984) 0.75

The effect of pH on the exchangeability with deuterium of protons coupled to molybdenum(V) in the active and the desulpho forms of xanthine oxidase. Biochem J (1983) 0.75

An attempt to cyanylate the thiol group of bovine serum albumin with 13C-enriched cyanide and to observe the thiocyanate carbon by 13C-NMR. Biochem Soc Trans (1998) 0.75

The effect of histidine-228 on the catalytic efficiency and stereospecificity of the serine hydroxymethyltransferase catalysed exchange of the alpha-protons of amino acids. Biochim Biophys Acta (1998) 0.75

The use of 13C n.m.r. and saturation transfer to detect tetrahedral intermediates in reactions catalysed by chymotrypsin and also in an amide inhibitor complex. Biochem Soc Trans (1994) 0.75

Studies on thiol proteinases with fluorescent probes [proceedings]. Biochem Soc Trans (1978) 0.75

A study of the pH dependence of the reaction of benzyloxycarbonyltryptophanylchloromethane with chymotrypsin. Biochem Soc Trans (1996) 0.75

A 1H-NMR study of the histidine resonance's of native subtilisin and of subtilisin inhibited by benzyloxycarbonylglycylglycylphenylalanyl-chloromethane. Biochem Soc Trans (1996) 0.75

Cyanylation of the thiol of the cysteine of flavodoxin from Anabaena PCC 7119. Biochem Soc Trans (1996) 0.75

A 13C-n.m.r. study of cyanylated beta-lactoglobulins. Biochem Soc Trans (1994) 0.75

Effect of the bulk solvent on the pKa values of the oxyanions of serine protease derivatives. Biochem Soc Trans (1998) 0.75

1H-NMb spectroscopy of beta-thiocyanatoalanine. Biochem Soc Trans (1996) 0.75

Synthesis of benzyloxycarbonylglycylglycinephenylalanine p-nitroanilide and an evaluation of its potential as a substrate for alpha-chymotrypsin and subtilisin BPN'. Biochem Soc Trans (1998) 0.75

Quenching of beta-lactoglobulin fluorescence by 2-nitro-5-thiobenzoic acid. Biochem Soc Trans (1998) 0.75