Published in Mol Microbiol on June 01, 1999
Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis--one species on the basis of genetic evidence. Appl Environ Microbiol (2000) 7.87
Identification of anthrax toxin genes in a Bacillus cereus associated with an illness resembling inhalation anthrax. Proc Natl Acad Sci U S A (2004) 4.73
Bacterial quorum sensing: its role in virulence and possibilities for its control. Cold Spring Harb Perspect Med (2012) 3.65
The genome sequence of Bacillus cereus ATCC 10987 reveals metabolic adaptations and a large plasmid related to Bacillus anthracis pXO1. Nucleic Acids Res (2004) 3.62
Pathogenomic sequence analysis of Bacillus cereus and Bacillus thuringiensis isolates closely related to Bacillus anthracis. J Bacteriol (2006) 3.23
A cell-cell signaling peptide activates the PlcR virulence regulon in bacteria of the Bacillus cereus group. EMBO J (2002) 2.66
Routine markerless gene replacement in Bacillus anthracis. Infect Immun (2006) 2.42
Complete sequence and organization of pBtoxis, the toxin-coding plasmid of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (2002) 2.18
Global effects of virulence gene regulators in a Bacillus anthracis strain with both virulence plasmids. Infect Immun (2003) 2.05
Bacterial adaptation through loss of function. PLoS Genet (2013) 1.99
The PlcR virulence regulon of Bacillus cereus. PLoS One (2008) 1.89
Characterization of Bacillus anthracis-like bacteria isolated from wild great apes from Cote d'Ivoire and Cameroon. J Bacteriol (2006) 1.77
The InhA2 metalloprotease of Bacillus thuringiensis strain 407 is required for pathogenicity in insects infected via the oral route. J Bacteriol (2002) 1.64
Use of single nucleotide polymorphisms in the plcR gene for specific identification of Bacillus anthracis. J Clin Microbiol (2005) 1.60
Role of N-terminal amino acids in the potency of anthrax lethal factor. PLoS One (2008) 1.54
Role of luxS in Bacillus anthracis growth and virulence factor expression. Virulence (2010) 1.52
Capsules, toxins and AtxA as virulence factors of emerging Bacillus cereus biovar anthracis. PLoS Negl Trop Dis (2015) 1.51
Bacterial endophthalmitis: therapeutic challenges and host-pathogen interactions. Prog Retin Eye Res (2007) 1.49
Requirement of flhA for swarming differentiation, flagellin export, and secretion of virulence-associated proteins in Bacillus thuringiensis. J Bacteriol (2002) 1.45
Structural Insights into Streptococcal Competence Regulation by the Cell-to-Cell Communication System ComRS. PLoS Pathog (2016) 1.42
Beta-lactamase genes of the penicillin-susceptible Bacillus anthracis Sterne strain. J Bacteriol (2003) 1.42
Bacillus anthracis multiplication, persistence, and genetic exchange in the rhizosphere of grass plants. Appl Environ Microbiol (2006) 1.42
Phosphatidylcholine-specific phospholipase C and sphingomyelinase activities in bacteria of the Bacillus cereus group. Infect Immun (2003) 1.37
Population structure of the Bacillus cereus group as determined by sequence analysis of six housekeeping genes and the plcR Gene. Infect Immun (2004) 1.33
Distinct mutations in PlcR explain why some strains of the Bacillus cereus group are nonhemolytic. J Bacteriol (2004) 1.32
Antagonistic Rgg regulators mediate quorum sensing via competitive DNA binding in Streptococcus pyogenes. MBio (2013) 1.32
Expanding the known repertoire of virulence factors produced by Bacillus cereus through early secretome profiling in three redox conditions. Mol Cell Proteomics (2010) 1.31
FlhA influences Bacillus thuringiensis PlcR-regulated gene transcription, protein production, and virulence. Appl Environ Microbiol (2005) 1.28
Relationship of plcR-regulated factors to Bacillus endophthalmitis virulence. Infect Immun (2003) 1.28
Differential proteomic analysis of the Bacillus anthracis secretome: distinct plasmid and chromosome CO2-dependent cross talk mechanisms modulate extracellular proteolytic activities. J Bacteriol (2006) 1.28
Evidence for contribution of tripartite hemolysin BL, phosphatidylcholine-preferring phospholipase C, and collagenase to virulence of Bacillus cereus endophthalmitis. Infect Immun (2000) 1.21
Necrotrophism is a quorum-sensing-regulated lifestyle in Bacillus thuringiensis. PLoS Pathog (2012) 1.21
Control of enterotoxin gene expression in Bacillus cereus F4430/73 involves the redox-sensitive ResDE signal transduction system. J Bacteriol (2006) 1.18
Molecular basis for group-specific activation of the virulence regulator PlcR by PapR heptapeptides. Nucleic Acids Res (2008) 1.16
The Bacillus thuringiensis PlcR-regulated gene inhA2 is necessary, but not sufficient, for virulence. J Bacteriol (2003) 1.15
Comparative transcriptional profiling of Bacillus cereus sensu lato strains during growth in CO2-bicarbonate and aerobic atmospheres. PLoS One (2009) 1.11
Low concentrations of bile salts induce stress responses and reduce motility in Bacillus cereus ATCC 14579 [corrected]. J Bacteriol (2007) 1.10
Distinct clpP genes control specific adaptive responses in Bacillus thuringiensis. J Bacteriol (2002) 1.10
Specificity and polymorphism of the PlcR-PapR quorum-sensing system in the Bacillus cereus group. J Bacteriol (2005) 1.09
IlsA, a unique surface protein of Bacillus cereus required for iron acquisition from heme, hemoglobin and ferritin. PLoS Pathog (2009) 1.08
Bacillus anthracis diverges from related clades of the Bacillus cereus group in 16S-23S ribosomal DNA intergenic transcribed spacers containing tRNA genes. Appl Environ Microbiol (2003) 1.08
Biofilm formation by Bacillus cereus is influenced by PlcR, a pleiotropic regulator. Appl Environ Microbiol (2006) 1.06
The redox regulator Fnr is required for fermentative growth and enterotoxin synthesis in Bacillus cereus F4430/73. J Bacteriol (2007) 1.06
A spontaneous translational fusion of Bacillus cereus PlcR and PapR activates transcription of PlcR-dependent genes in Bacillus anthracis via binding with a specific palindromic sequence. Infect Immun (2004) 1.03
CwpFM (EntFM) is a Bacillus cereus potential cell wall peptidase implicated in adhesion, biofilm formation, and virulence. J Bacteriol (2010) 1.01
The alternative sigma factor sigmaH is required for toxin gene expression by Bacillus anthracis. J Bacteriol (2006) 1.01
Biosurfactant production and surface translocation are regulated by PlcR in Bacillus cereus ATCC 14579 under low-nutrient conditions. Appl Environ Microbiol (2007) 1.00
Structural basis for the activation mechanism of the PlcR virulence regulator by the quorum-sensing signal peptide PapR. Proc Natl Acad Sci U S A (2012) 1.00
The pore-forming haemolysins of bacillus cereus: a review. Toxins (Basel) (2013) 0.98
From genome to toxicity: a combinatory approach highlights the complexity of enterotoxin production in Bacillus cereus. Front Microbiol (2015) 0.98
Massive horizontal gene transfer, strictly vertical inheritance and ancient duplications differentially shape the evolution of Bacillus cereus enterotoxin operons hbl, cytK and nhe. BMC Evol Biol (2015) 0.97
Identification of a protein subset of the anthrax spore immunome in humans immunized with the anthrax vaccine adsorbed preparation. Infect Immun (2005) 0.97
Plasmid-encoded regulator of extracellular proteases in Bacillus anthracis. J Bacteriol (2005) 0.97
The YvfTU two-component system is involved in plcR expression in Bacillus cereus. BMC Microbiol (2008) 0.96
ApoFnr binds as a monomer to promoters regulating the expression of enterotoxin genes of Bacillus cereus. J Bacteriol (2008) 0.96
A Bacillus thuringiensis S-layer protein involved in toxicity against Epilachna varivestis (Coleoptera: Coccinellidae). Appl Environ Microbiol (2006) 0.95
Bacillus cereus-induced permeability of the blood-ocular barrier during experimental endophthalmitis. Invest Ophthalmol Vis Sci (2009) 0.95
ResDE-dependent regulation of enterotoxin gene expression in Bacillus cereus: evidence for multiple modes of binding for ResD and interaction with Fnr. J Bacteriol (2009) 0.95
Bacillus anthracis secretome time course under host-simulated conditions and identification of immunogenic proteins. Proteome Sci (2007) 0.93
Identification of the main promoter directing cereulide biosynthesis in emetic Bacillus cereus and its application for real-time monitoring of ces gene expression in foods. Appl Environ Microbiol (2009) 0.93
Temperature-dependent production of various PlcR-controlled virulence factors in Bacillus weihenstephanensis strain KBAB4. Appl Environ Microbiol (2012) 0.91
Transcriptional analysis of the toxin-coding plasmid pBtoxis from Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (2006) 0.91
A ferric dicitrate uptake system is required for the full virulence of Bacillus cereus. Curr Microbiol (2005) 0.90
An extracytoplasmic function sigma factor controls beta-lactamase gene expression in Bacillus anthracis and other Bacillus cereus group species. J Bacteriol (2009) 0.90
Quorum sensing in Bacillus thuringiensis is required for completion of a full infectious cycle in the insect. Toxins (Basel) (2014) 0.89
Lactate dehydrogenase A promotes communication between carbohydrate catabolism and virulence in Bacillus cereus. J Bacteriol (2011) 0.89
plcR papR-independent expression of anthrolysin O by Bacillus anthracis. J Bacteriol (2006) 0.88
Anthrax Toxin-Expressing Bacillus cereus Isolated from an Anthrax-Like Eschar. PLoS One (2016) 0.87
The pore-forming protein Cry5B elicits the pathogenicity of Bacillus sp. against Caenorhabditis elegans. PLoS One (2011) 0.87
PapR peptide maturation: role of the NprB protease in Bacillus cereus 569 PlcR/PapR global gene regulation. FEMS Immunol Med Microbiol (2009) 0.86
Cell Differentiation in a Bacillus thuringiensis Population during Planktonic Growth, Biofilm Formation, and Host Infection. MBio (2015) 0.86
Cloning and identification of a gene encoding spore cortex-lytic enzyme in Bacillus thuringiensis. Curr Microbiol (2007) 0.86
SinR controls enterotoxin expression in Bacillus thuringiensis biofilms. PLoS One (2014) 0.85
Camelysin is a novel surface metalloproteinase from Bacillus cereus. Infect Immun (2004) 0.85
Erythrophore cell response to food-associated pathogenic bacteria: implications for detection. Microb Biotechnol (2008) 0.85
Activation of the latent PlcR regulon in Bacillus anthracis. Microbiology (2010) 0.84
Strategy for identification of Bacillus cereus and Bacillus thuringiensis strains closely related to Bacillus anthracis. Appl Environ Microbiol (2006) 0.84
Investigating the genome diversity of B. cereus and evolutionary aspects of B. anthracis emergence. Genomics (2011) 0.83
Characterization of a small PlcR-regulated gene co-expressed with cereolysin O. BMC Microbiol (2007) 0.83
Production, secretion and biological activity of Bacillus cereus enterotoxins. Toxins (Basel) (2010) 0.82
Bacillus cereus induces permeability of an in vitro blood-retina barrier. Infect Immun (2008) 0.82
Distribution of genes encoding putative virulence factors and fragment length polymorphisms in the vrrA gene among Brazilian isolates of Bacillus cereus and Bacillus thuringiensis. Appl Environ Microbiol (2005) 0.81
Bacterial Cell-Cell Communication in the Host via RRNPP Peptide-Binding Regulators. Front Microbiol (2016) 0.81
Structural Mechanisms of Peptide Recognition and Allosteric Modulation of Gene Regulation by the RRNPP Family of Quorum-Sensing Regulators. J Mol Biol (2016) 0.81
Differential secretomics of Streptococcus pyogenes reveals a novel peroxide regulator (PerR)-regulated extracellular virulence factor mitogen factor 3 (MF3). Mol Cell Proteomics (2011) 0.80
N-acetylglucosamine deacetylases modulate the anchoring of the gamma-glutamyl capsule to the cell wall of Bacillus anthracis. Microb Drug Resist (2014) 0.80
PlcRa, a new quorum-sensing regulator from Bacillus cereus, plays a role in oxidative stress responses and cysteine metabolism in stationary phase. PLoS One (2012) 0.79
Discovery of Bacillus thuringiensis virulence genes using signature-tagged mutagenesis in an insect model of septicaemia. Curr Microbiol (2006) 0.78
CodY Regulates the Activity of the Virulence Quorum Sensor PlcR by Controlling the Import of the Signaling Peptide PapR in Bacillus thuringiensis. Front Microbiol (2016) 0.78
Phospholipase C in Beijing strains of Mycobacterium tuberculosis. Iran J Microbiol (2010) 0.78
Characterization of two Bacillus thuringiensis genes identified by in vivo screening of virulence factors. Appl Environ Microbiol (2004) 0.76
Comparative Bioinformatics and Experimental Analysis of the Intergenic Regulatory Regions of Bacillus cereus hbl and nhe Enterotoxin Operons and the Impact of CodY on Virulence Heterogeneity. Front Microbiol (2016) 0.76
Over-expression, purification, and confirmation of Bacillus anthracis transcriptional regulator NprR. Protein Expr Purif (2015) 0.76
Cloning, Purification and Characterization of the Collagenase ColA Expressed by Bacillus cereus ATCC 14579. PLoS One (2016) 0.75
Cytochrome c551 and the cytochrome c maturation pathway affect virulence gene expression in Bacillus cereus ATCC 14579. J Bacteriol (2014) 0.75
Automated ribotyping and antibiotic resistance determining of Bacillus spp from conjunctiva of diabetic patients. Iran J Basic Med Sci (2014) 0.75
Experimental Validation of Bacillus anthracis A16R Proteogenomics. Sci Rep (2015) 0.75
Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev (1998) 13.47
Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis--one species on the basis of genetic evidence. Appl Environ Microbiol (2000) 7.87
Construction of cloning vectors for Bacillus thuringiensis. Gene (1991) 4.58
Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol Mol Biol Rev (1998) 4.14
Fluorescent Amplified Fragment Length Polymorphism Analysis of Norwegian Bacillus cereus and Bacillus thuringiensis Soil Isolates. Appl Environ Microbiol (2001) 2.85
Structural and functional analysis of Tn4430: identification of an integrase-like protein involved in the co-integrate-resolution process. EMBO J (1988) 2.74
Genotypic Diversity among Bacillus cereus and Bacillus thuringiensis Strains. Appl Environ Microbiol (1994) 2.58
Genetic structure of population of Bacillus cereus and B. thuringiensis isolates associated with periodontitis and other human infections. J Clin Microbiol (2000) 2.39
Genetic diversity of Bacillus cereus/B. thuringiensis isolates from natural sources. Curr Microbiol (1998) 2.21
The incompatibility between the PlcR- and AtxA-controlled regulons may have selected a nonsense mutation in Bacillus anthracis. Mol Microbiol (2001) 2.20
The plcR regulon is involved in the opportunistic properties of Bacillus thuringiensis and Bacillus cereus in mice and insects. Microbiology (2000) 2.09
Overproduction of encapsulated insecticidal crystal proteins in a Bacillus thuringiensis spo0A mutant. Biotechnology (N Y) (1995) 1.97
Oligopeptide permease is required for expression of the Bacillus thuringiensis plcR regulon and for virulence. Mol Microbiol (2001) 1.95
Identification of a Bacillus thuringiensis gene that positively regulates transcription of the phosphatidylinositol-specific phospholipase C gene at the onset of the stationary phase. J Bacteriol (1996) 1.94
How does Bacillus thuringiensis produce so much insecticidal crystal protein? J Bacteriol (1995) 1.85
Structural and functional analysis of the promoter region involved in full expression of the cryIIIA toxin gene of Bacillus thuringiensis. Mol Microbiol (1994) 1.76
Sequence analysis of three Bacillus cereus loci carrying PIcR-regulated genes encoding degradative enzymes and enterotoxin. Microbiology (1999) 1.58
A transposon-like structure related to the delta-endotoxin gene of Bacillus thuringiensis. EMBO J (1984) 1.57
Isolation of a DNA sequence related to several plasmids from Bacillus thuringiensis after a mating involving the Streptococcus faecalis plasmid pAM beta 1. Mol Gen Genet (1983) 1.57
STAB-SD: a Shine-Dalgarno sequence in the 5' untranslated region is a determinant of mRNA stability. Mol Microbiol (1996) 1.56
Multiplicity of delta-endotoxin genes with different insecticidal specificities in Bacillus thuringiensis aizawai 7.29. Mol Microbiol (1988) 1.54
Cell biology of tissue factor, the principal initiator of blood coagulation. Thromb Res (1996) 1.52
Characterization of plasmid pAW63, a second self-transmissible plasmid in Bacillus thuringiensis subsp. kurstaki HD73. Microbiology (1998) 1.52
Molecular relationships among plasmids of Bacillus thuringiensis: conserved sequences through 11 crystalliferous strains. Mol Gen Genet (1982) 1.49
A complete physical map of a Bacillus thuringiensis chromosome. J Bacteriol (1993) 1.47
Physical maps of the genomes of three Bacillus cereus strains. J Bacteriol (1992) 1.41
Regulation of toxin and virulence gene transcription in Bacillus thuringiensis. Int J Med Microbiol (2000) 1.37
Genome organization is not conserved between Bacillus cereus and Bacillus subtilis. Microbiology (1999) 1.36
Construction of Novel Bacillus thuringiensis Strains with Different Insecticidal Activities by Transduction and Transformation. Appl Environ Microbiol (1992) 1.33
Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active delta-endotoxin gene of Bacillus thuringiensis aizawai 7.29. Mol Microbiol (1989) 1.31
Identification of Tn4430, a transposon of Bacillus thuringiensis functional in Escherichia coli. Mol Gen Genet (1986) 1.30
Expression in Bacillus subtilis of the Bacillus thuringiensis cryIIIA toxin gene is not dependent on a sporulation-specific sigma factor and is increased in a spo0A mutant. J Bacteriol (1994) 1.27
Molecular cloning of a putative novel human bZIP transcription factor on chromosome 17q22. Genomics (1994) 1.27
Construction of new insecticidal Bacillus thuringiensis recombinant strains by using the sporulation non-dependent expression system of cryIIIA and a site specific recombination vector. J Biotechnol (1996) 1.19
Analysis of cryIAa expression in sigE and sigK mutants of Bacillus thuringiensis. Mol Gen Genet (1996) 1.19
Variable numbers of rRNA gene operons in Bacillus cereus strains. FEMS Microbiol Lett (1996) 1.17
Molecular basis for group-specific activation of the virulence regulator PlcR by PapR heptapeptides. Nucleic Acids Res (2008) 1.16
Structural and genetic organization of IS232, a new insertion sequence of Bacillus thuringiensis. J Bacteriol (1990) 1.16
Full expression of the cryIIIA toxin gene of Bacillus thuringiensis requires a distant upstream DNA sequence affecting transcription. J Bacteriol (1993) 1.14
A small (2.4 Mb) Bacillus cereus chromosome corresponds to a conserved region of a larger (5.3 Mb) Bacillus cereus chromosome. Mol Microbiol (1994) 1.11
Replication mechanism and sequence analysis of the replicon of pAW63, a conjugative plasmid from Bacillus thuringiensis. J Bacteriol (1999) 1.09
Comparative analysis of the virulence of invertebrate and mammalian pathogenic bacteria in the oral insect infection model Galleria mellonella. J Invertebr Pathol (2009) 1.06
TCF11/Nrf1 overexpression increases the intracellular glutathione level and can transactivate the gamma-glutamylcysteine synthetase (GCS) heavy subunit promoter. Biochim Biophys Acta (2001) 1.05
Identification of genes involved in the activation of the Bacillus thuringiensis inhA metalloprotease gene at the onset of sporulation. Microbiology (2001) 1.04
Distribution of S-layers on the surface of Bacillus cereus strains: phylogenetic origin and ecological pressure. Environ Microbiol (2001) 1.03
Small Maf proteins interact with the human transcription factor TCF11/Nrf1/LCR-F1. Nucleic Acids Res (1996) 1.03
The dlt operon of Bacillus cereus is required for resistance to cationic antimicrobial peptides and for virulence in insects. J Bacteriol (2009) 1.02
Identification of asialo GM1 as a binding structure for Escherichia coli colonization factor antigens. FEMS Microbiol Lett (1990) 0.98
A tight cluster of five unrelated human genes on chromosome 16q22.1. Hum Mol Genet (1993) 0.98
CpG islands in mammalian gene promoters are inherently resistant to de novo methylation. Genet Anal Tech Appl (1992) 0.97
Genome structure and evolution of the Bacillus cereus group. Curr Top Microbiol Immunol (2002) 0.97
A recombinase-mediated system for elimination of antibiotic resistance gene markers from genetically engineered Bacillus thuringiensis strains. Appl Environ Microbiol (1997) 0.96
Insertional inactivation of hblC encoding the L2 component of Bacillus cereus ATCC 14579 haemolysin BL strongly reduces enterotoxigenic activity, but not the haemolytic activity against human erythrocytes. Microbiology (1999) 0.95
Identification of a delta-Endotoxin Gene Product Specifically Active against Spodoptera littoralis Bdv. among Proteolysed Fractions of the Insecticidal Crystals of Bacillus thuringiensis subsp. aizawai 7.29. Appl Environ Microbiol (1988) 0.95
Opposite sorting of tissue factor in human umbilical vein endothelial cells and Madin-Darby canine kidney epithelial cells. Blood (1996) 0.94
The chromosome map of Bacillus thuringiensis subsp. canadensis HD224 is highly similar to that of the Bacillus cereus type strain ATCC 14579. FEMS Microbiol Lett (1996) 0.94
Bacillus popilliae cry18Aa operon is transcribed by sigmaE and sigmaK forms of RNA polymerase from a single initiation site. Nucleic Acids Res (1998) 0.92
Salivary lactoferrin in a selected group of subjects with exceptional extrinsic dental staining. J Oral Pathol (1987) 0.91
Structural organization and mapping of the human TCF11 gene. Genomics (1995) 0.86
Cloning of a gene from Bacillus cereus with homology to the mreB gene from Escherichia coli. Gene (1992) 0.86
BceS1, a new addition to the type III restriction and modification family. FEMS Microbiol Lett (2001) 0.85
Recent aspects of genetic manipulation in Bacillus thuringiensis. Biochimie (1985) 0.84
A genetic system that reports transient activation of genes in Bacillus. Gene (1997) 0.82
Development and field performance of a broad-spectrum nonviable asporogenic recombinant strain of Bacillus thuringiensis with greater potency and UV resistance. Appl Environ Microbiol (1999) 0.81
Insertional inactivation of a Tet(K)/Tet(L) like transporter does not eliminate tetracycline resistance in Bacillus cereus. FEMS Microbiol Lett (1997) 0.81
Characterization of the chemotaxis fli Y and che A genes in Bacillus cereus. FEMS Microbiol Lett (2000) 0.81
Physical map of the genome of the green phototrophic bacterium Chlorobium tepidum. J Bacteriol (1995) 0.80
Genetic analysis of cryIIIA gene expression in Bacillus thuringiensis. Microbiology (1996) 0.80
Tissue-specific methylation of a CpG island in transgenic mice. Gene (1992) 0.79
Physical map of alkaliphilic Bacillus firmus OF4 and detection of a large endogenous plasmid. Extremophiles (1998) 0.79
A Bacillus cereus member of the SNF2 family. Microbiology (1997) 0.78
[Bacillus thuringiensis: a biotechnology model]. J Soc Biol (1999) 0.76
Differential methylation of a CpG-island concatemer in hemi- and homozygous transgenic mice. FEBS Lett (1991) 0.76
A type-III DNA restriction and modification system in Bacillus cereus? Gene (1992) 0.75
Colony inhibiting factor in mature granulocytes from normal individuals and patients with chronic myeloid leukemia. Eur J Haematol (1987) 0.75
The gene for human transcription factor TCF11 is located telomeric to D17S1827, BTR and HP1Hsbeta on chromosome 17q22. Genet Anal (1999) 0.75
[Influenza vaccination. Measurement of antibodies in blood samples before and after vaccination]. Tidsskr Nor Laegeforen (1985) 0.75