A mutant Escherichia coli primase defective in elongation of primer RNA chains.

PubWeight™: 0.81‹?›

🔗 View Article (PMC 93854)

Published in J Bacteriol on June 01, 1999

Authors

W Sun1, J Schoneich, G N Godson

Author Affiliations

1: Biochemistry Department, New York University School of Medicine, New York, New York 10016, USA.

Articles cited by this

Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol (1990) 61.47

Transcription processivity: protein-DNA interactions holding together the elongation complex. Science (1996) 2.58

An integrated model of the transcription complex in elongation, termination, and editing. Science (1998) 2.51

Organization and evolution of bacterial and bacteriophage primase-helicase systems. J Mol Evol (1992) 2.19

The RNA primer synthesized by primase to initiate phage G4 DNA replication. J Biol Chem (1978) 2.03

A general priming system employing only dnaB protein and primase for DNA replication. Proc Natl Acad Sci U S A (1979) 1.85

dnaG (primase)-dependent origins of DNA replication. Nucleotide sequences of the negative strand initiation sites of bacteriophages St-1, phi K, and alpha 3. J Biol Chem (1979) 1.82

Identification of a domain of Escherichia coli primase required for functional interaction with the DnaB helicase at the replication fork. J Biol Chem (1994) 1.80

A ribo-deoxyribonucleotide primer synthesized by primase. J Biol Chem (1978) 1.49

Mapping of the priming substrate contacts in the active center of Escherichia coli RNA polymerase. J Biol Chem (1991) 1.41

Initiation of DNA replication by the Escherichia coli dnaG protein: evidence that tertiary structure is involved. Proc Natl Acad Sci U S A (1980) 1.40

DNA or RNA priming of bacteriophage G4 DNA synthesis by Escherichia coli dnaG protein. Proc Natl Acad Sci U S A (1977) 1.37

Evidence that discontinuous DNA replication in Escherichia coli is primed by approximately 10 to 12 residues of RNA starting with a purine. J Mol Biol (1985) 1.20

A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription. J Mol Biol (1997) 1.15

Biochemical characterization of Escherichia coli temperature-sensitive dnaB mutants dnaB8, dnaB252, dnaB70, dnaB43, and dnaB454. J Bacteriol (1995) 1.11

Specificity of recognition sequence for Escherichia coli primase. Mol Gen Genet (1991) 1.09

Primase from Escherichia coli primes single-stranded templates in the absence of single-stranded DNA-binding protein or other auxiliary proteins. Template sequence requirements based on the bacteriophage G4 complementary strand origin and Okazaki fragment initiation sites. J Biol Chem (1993) 1.06

Domains of Escherichia coli primase: functional activity of a 47-kDa N-terminal proteolytic fragment. Proc Natl Acad Sci U S A (1994) 1.06

Mutational analysis of the primer RNA template region in the replication origin (oric) of bacteriophage G4: priming signal recognition by Escherichia coli primase. Gene (1989) 1.00

Studies of the functional topography of the catalytic center of Escherichia coli primase. J Biol Chem (1995) 0.93

Structure of the Escherichia coli primase/single-strand DNA-binding protein/phage G4oric complex required for primer RNA synthesis. J Mol Biol (1998) 0.88

Binding and phasing of Escherichia coli single-stranded DNA-binding protein by the secondary structure of phage G4 origin of complementary DNA strand synthesis (G4oric). J Biol Chem (1993) 0.88

An over-expression plasmid for Escherichia coli primase. Gene (1991) 0.87

The Haemophilus influenzae dnaG sequence and conserved bacterial primase motifs. Gene (1993) 0.87

Interaction of Escherichia coli primase with a phage G4ori(c)-E. coli SSB complex. J Bacteriol (1996) 0.84

Cloning and analysis of the dnaG gene encoding Pseudomonas putida DNA primase. Biochim Biophys Acta (1997) 0.82

Articles by these authors

Nucleotide sequence of bacteriophage G4 DNA. Nature (1978) 10.46

Lysis of Escherichia coli with a neutral detergent. Biochim Biophys Acta (1967) 8.51

Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli. Proc Natl Acad Sci U S A (1983) 5.79

A simple method of preparing large amounts of phiX174 RF 1 supercoiled DNA. Biochim Biophys Acta (1973) 5.15

Specificity of the S1 nuclease from Aspergillus oryzae. J Biol Chem (1975) 3.57

Use of Brij lysis as a general method to prepare polyribosomes from Escherichia coli. Biochim Biophys Acta (1967) 2.82

Structure of the plasmodium knowlesi gene coding for the circumsporozoite protein. Cell (1983) 2.60

Nucleotide sequences of the separate origins of synthesis of bacteriophage G4 viral and complementary DNA strands. Proc Natl Acad Sci U S A (1978) 2.41

Mutants of Escherichia coli with temperature-sensitive lesions in membrane phospholipid synthesis: genetic analysis of glycerol-3-phosphate acyltransferase mutants. Mol Gen Genet (1972) 2.02

Isolation and characterization of an Escherichia coli K-12 mutant with a temperature-sensitive recA- phenotype. J Bacteriol (1974) 2.01

Evolution of phi-chi 174. Isolation of four new phi-chi-like phages and comparison with phi-chi 174. Virology (1974) 1.99

Sequences of the Escherichia coli dnaG primase gene and regulation of its expression. Proc Natl Acad Sci U S A (1982) 1.98

Identification and chemical synthesis of a tandemly repeated immunogenic region of Plasmodium knowlesi circumsporozoite protein. Nature (1983) 1.90

Cloning and expression in E. coli of the malarial sporozoite surface antigen gene from Plasmodium knowlesi. Nature (1983) 1.90

Promotion, termination, and anti-termination in the rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli K-12. Mol Gen Genet (1984) 1.84

Susceptibility of the phiX-like phages G4 and G14 to R-EcoRi endonuclease. Virology (1974) 1.71

Cloning and nucleotide sequence of a chromosomally encoded tetracycline resistance determinant, tetA(M), from a pathogenic, methicillin-resistant strain of Staphylococcus aureus. Antimicrob Agents Chemother (1990) 1.67

dna, single stranded/*metab. Virology (1976) 1.66

Gene K, a new overlapping gene in bacteriophage G4. Nature (1978) 1.66

Simple centrifugation method for rapid separation of sporozoites from mosquitoes. J Parasitol (1984) 1.62

Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12. Mol Gen Genet (1983) 1.44

Plasmodium knowlesi sporozoite antigen: expression by infectious recombinant vaccinia virus. Science (1984) 1.42

The replication of bacteriophage MS2. VI. Interaction between bacteriophage RNA and cellular components in MS2-infected Escherichia coli. J Mol Biol (1967) 1.40

Characterization and synthesis of phi X174 proteins in ultraviolet-irradiated and unirradiated cells. J Mol Biol (1971) 1.38

Action of the single-stranded DNA specific nuclease S1 on double-stranded DNA. Biochim Biophys Acta (1973) 1.37

Cloning and characterization of the Escherichia coli chromosomal region surrounding the dnaG Gene, with a correlated physical and genetic map of dnaG generated via transposon Tn5 mutagenesis. Mol Gen Genet (1982) 1.27

Origin and direction phiX174 double- and single-stranded DNA synthesis. J Mol Biol (1974) 1.26

Possible new genes as revealed by molecular analysis of a 5-kb Escherichia coli chromosomal region 5' to the rpsU-dnaG-rpoD macromolecular-synthesis operon. Gene (1987) 1.25

The rpsU-dnaG-rpoD macromolecular synthesis operon of E. coli. Cell (1984) 1.25

Cloning, sequencing, and species relatedness of the Escherichia coli cca gene encoding the enzyme tRNA nucleotidyltransferase. J Biol Chem (1986) 1.22

A simple apparatus for rapid isoelectrofocusing of multiple samples on a micro scale. Anal Biochem (1970) 1.20

Radiation-induced base substitution mutagenesis in single-stranded DNA phage M13. Nature (1981) 1.18

Biochemical characterization of Escherichia coli temperature-sensitive dnaB mutants dnaB8, dnaB252, dnaB70, dnaB43, and dnaB454. J Bacteriol (1995) 1.11

Evolution of phi chi 174. II. A cleavage map of the G4 phage genome and comparison with the cleavage map of phi chi 174. Virology (1975) 1.09

Identification of a phiX174 coded protein involved in the shut-off of host DNA replication. Biochem Biophys Res Commun (1975) 1.06

Domains of Escherichia coli primase: functional activity of a 47-kDa N-terminal proteolytic fragment. Proc Natl Acad Sci U S A (1994) 1.06

A restriction endonuclease cleavage map of G4. Virology (1978) 1.04

Diversity of circumsporozoite antigen genes from two strains of the malarial parasite Plasmodium knowlesi. Science (1985) 1.02

A temperature-dependent pBR322 copy number mutant resulting from a Tn5 position effect. Proc Natl Acad Sci U S A (1986) 1.01

Mutational analysis of the primer RNA template region in the replication origin (oric) of bacteriophage G4: priming signal recognition by Escherichia coli primase. Gene (1989) 1.00

Site of synthesis of viral ribonucleic acid and phage assembly in MS2-infected Escherichia coli. J Mol Biol (1968) 1.00

Isolation and partial characterization of temperature-sensitive mutants in ten loci that affect cell membrane synthesis in Escherichia coli: isolation and genetic sorting. J Bacteriol (1973) 0.99

Structure of an immunodominant epitope of the circumsporozoite surface protein of Plasmodium knowlesi. Biochemistry (1984) 0.99

Immunogenicity of the repetitive and nonrepetitive peptide regions of the divergent CS protein of Plasmodium knowlesi. J Immunol (1986) 0.97

Organization and expression of the Plasmodium knowlesi circumsporozoite antigen gene. Mol Biochem Parasitol (1987) 0.97

G4 DNA replication. I. Origin of synthesis of the viral and complementary DNA strands. J Mol Biol (1977) 0.97

Distinct functional contributions of three potential secondary structures in the phage G4 origin of complementary DNA strand synthesis. Gene (1989) 0.95

Nucleotide sequence of the J gene and surrounding untranslated regions of phage G4 DNA: comparison with phage phiX174. Cell (1978) 0.95

Studies of the functional topography of the catalytic center of Escherichia coli primase. J Biol Chem (1995) 0.93

Evolution of the three overlapping gene systems in G4 and phi X174. J Mol Biol (1979) 0.93

Specificity of Tn5 insertions into a 36-bp DNA sequence repeated in tandem seven times. Gene (1984) 0.92

Structure of Escherichia coli ribosomes: effect of ribonuclease on the 30-S and 50-S subunits. Biochim Biophys Acta (1970) 0.91

Structural features of the priming signal recognized by primase: mutational analysis of the phage G4 origin of complementary DNA strand synthesis. Nucleic Acids Res (1990) 0.91

DNA----DNA, and DNA----RNA----protein: orchestration by a single complex operon. Bioessays (1989) 0.89

The DNA sequence on bacteriophage G4 recognized by the Escherichia coli B restriction enzyme. J Mol Biol (1979) 0.89

Selective decay of Escherichia coli dnaG messenger RNA is initiated by RNase E. J Biol Chem (1993) 0.89

Structure of the Escherichia coli primase/single-strand DNA-binding protein/phage G4oric complex required for primer RNA synthesis. J Mol Biol (1998) 0.88

Binding and phasing of Escherichia coli single-stranded DNA-binding protein by the secondary structure of phage G4 origin of complementary DNA strand synthesis (G4oric). J Biol Chem (1993) 0.88

Isolation of alpha- and beta-tubulin genes of Plasmodium falciparum using a single oligonucleotide probe. Mol Biochem Parasitol (1990) 0.88

Molecular approaches to malaria vaccines. Sci Am (1985) 0.87

Overlapping genes in bacteriophages phiX174 and G4. Biochem Soc Trans (1978) 0.87

G4 DNA replication. III. Synthesis of replicative form. J Mol Biol (1977) 0.87

Replication origin (oric) on the complementary DNA strand of Escherichia coli phage G4: biological properties of mutants. Gene (1987) 0.87

Specific inhibition of phospholipid synthesis in plsA mutants of Escherichia coli. J Bacteriol (1976) 0.87

Interaction of Escherichia coli primase with a phage G4ori(c)-E. coli SSB complex. J Bacteriol (1996) 0.84

Isolation and construction of mutants of the G4 minus strand origin: analysis of their in vivo activity. Biochim Biophys Acta (1985) 0.84

Expression of the major surface antigen of Plasmodium knowlesi sporozoites in yeast. Science (1985) 0.83

Evolution of bacteriophage phi X174. IV. Restriction enzyme cleavage map of St-1. J Virol (1978) 0.83

DNA heteroduplex analysis of the relation between bacteriophages phiX174 and S.13. J Mol Biol (1973) 0.83

Phi X gene expression in UV-irradiated cells treated with chloramphenicol. Virology (1971) 0.82

G4 DNA replication. II. Synthesis of viral progeny single-stranded DNA. J Mol Biol (1977) 0.82

Evolution of phiX174 III. Restriction map of S13 and its alignment with that of phiX174. Virology (1976) 0.81

G4 DNA replication in vivo and the origins of its strand synthesis. Cold Spring Harb Symp Quant Biol (1979) 0.81

Role of the 5' upstream sequence and tandem promoters in regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon. J Bacteriol (1988) 0.81

Biosynthesis of nucleic acids in Bacillus megaterium. 4. Roles of the 'nuclear' cytoplasmic and cytoplasmic-membrane components of the cell in the biosynthesis of ribonucleic acid. Biochem J (1964) 0.80

Comparison of left-end DNA sequences of bacteriophages Mu and D108. Gene (1985) 0.80

In vitro stimulation of Escherichia coli RNA polymerase sigma subunit synthesis by NusA protein. Gene (1985) 0.80

Role of the potential secondary structures in phage G4 origin of complementary DNA strand synthesis. Gene (1988) 0.79

Hypersensitive mung bean nuclease cleavage sites in Plasmodium knowlesi DNA. Gene (1990) 0.79

An Escherichia coli cis-acting antiterminator sequence: the dnaG nut site. Mol Gen Genet (1989) 0.78

Structure and organization of genes for sporozoite surface antigens. Philos Trans R Soc Lond B Biol Sci (1984) 0.78

Localization of a Plasmodium surface antigen epitope by Tn5 mutagenesis mapping of a recombinant cDNA clone. Science (1983) 0.77

Synthesis of polyribonucleotide chains from the 3'-hydroxyl terminus of oligodeoxynucleotides by Escherichia coli primase. J Biol Chem (1998) 0.77

Comparison of the G4 and phiX174 phage genomes by electron microscopy. Virology (1979) 0.76

Evolution of bacteriophage phi C174. V. Alignment of the phi X174, G4, and St-1 restriction enzyme cleavage maps. J Virol (1978) 0.75

Primed synthesis methods of sequencing DNA and RNA. Fed Proc (1980) 0.75

Expression of the Plasmodium knowlesi circumsporozoite antigen in Escherichia coli directed by Plasmodium bacterial-like promoter sequences. Gene (1986) 0.75