PubRank

T J Silhavy

Author PubWeight™ 285.36‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Genetic analysis of the ompB locus in Escherichia coli K-12. J Mol Biol 1981 8.18
2 The ompB locus and the regulation of the major outer membrane porin proteins of Escherichia coli K12. J Mol Biol 1981 7.69
3 Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm. J Bacteriol 1979 6.38
4 Suppressor mutations that restore export of a protein with a defective signal sequence. Cell 1981 6.33
5 Mechanisms of protein localization. Microbiol Rev 1983 6.12
6 Genetic analysis of the major outer membrane proteins of Escherichia coli. Annu Rev Genet 1981 5.30
7 Maltose transport in Escherichia coli K12. A comparison of transport kinetics in wild-type and lambda-resistant mutants as measured by fluorescence quenching. Eur J Biochem 1976 5.25
8 Conversion of beta-galactosidase to a membrane-bound state by gene fusion. Proc Natl Acad Sci U S A 1976 4.61
9 Uses of lac fusions for the study of biological problems. Microbiol Rev 1985 4.38
10 Phosphorylation and dephosphorylation of a bacterial transcriptional activator by a transmembrane receptor. Genes Dev 1989 4.05
11 Transcriptional regulation of Escherichia coli K-12 major outer membrane protein 1b. J Bacteriol 1979 3.85
12 Use of gene fusions to study outer membrane protein localization in Escherichia coli. Proc Natl Acad Sci U S A 1977 3.78
13 Mutations affecting localization of an Escherichia coli outer membrane protein, the bacteriophage lambda receptor. J Mol Biol 1980 3.62
14 The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP. Genes Dev 1995 3.61
15 Transposable lambda placMu bacteriophages for creating lacZ operon fusions and kanamycin resistance insertions in Escherichia coli. J Bacteriol 1985 3.54
16 Dominant constitutive mutations in malT, the positive regulator gene of the maltose regulon in Escherichia coli. J Mol Biol 1978 3.46
17 The E. coli ffh gene is necessary for viability and efficient protein export. Nature 1992 3.45
18 Sequence analysis of mutations that prevent export of lambda receptor, an Escherichia coli outer membrane protein. Nature 1980 3.36
19 Periplasmic stress and ECF sigma factors. Annu Rev Microbiol 2001 3.21
20 Open reading frame expression vectors: a general method for antigen production in Escherichia coli using protein fusions to beta-galactosidase. Proc Natl Acad Sci U S A 1983 3.09
21 EnvZ, a transmembrane environmental sensor of Escherichia coli K-12, is phosphorylated in vitro. J Bacteriol 1988 3.04
22 cis-acting ompF mutations that result in OmpR-dependent constitutive expression. J Bacteriol 1991 2.99
23 A mechanism of protein localization: the signal hypothesis and bacteria. J Cell Biol 1980 2.91
24 From acids to osmZ: multiple factors influence synthesis of the OmpF and OmpC porins in Escherichia coli. Mol Microbiol 1996 2.86
25 The sigma(E) and the Cpx signal transduction systems control the synthesis of periplasmic protein-folding enzymes in Escherichia coli. Genes Dev 1997 2.84
26 A previously unidentified gene in the spc operon of Escherichia coli K12 specifies a component of the protein export machinery. Cell 1982 2.84
27 Structure of the malB region in Escherichia coli K12. II. Genetic map of the malE,F,G operon. Mol Gen Genet 1979 2.83
28 Genetic analysis of protein export in Escherichia coli K12. Annu Rev Biochem 1985 2.78
29 EnvZ controls the concentration of phosphorylated OmpR to mediate osmoregulation of the porin genes. J Mol Biol 1991 2.78
30 The response regulator SprE controls the stability of RpoS. Proc Natl Acad Sci U S A 1996 2.71
31 Labeling of proteins with beta-galactosidase by gene fusion. Identification of a cytoplasmic membrane component of the Escherichia coli maltose transport system. J Biol Chem 1980 2.63
32 Isolation and characterization of chain-terminating nonsense mutations in a porin regulator gene, envZ. J Bacteriol 1983 2.61
33 A bacterial environmental sensor that functions as a protein kinase and stimulates transcriptional activation. Genes Dev 1989 2.60
34 Transduction of envelope stress in Escherichia coli by the Cpx two-component system. J Bacteriol 1997 2.56
35 Overproduction of NlpE, a new outer membrane lipoprotein, suppresses the toxicity of periplasmic LacZ by activation of the Cpx signal transduction pathway. J Bacteriol 1995 2.52
36 The chaperone-assisted membrane release and folding pathway is sensed by two signal transduction systems. EMBO J 1997 2.52
37 Lambda placMu: a transposable derivative of bacteriophage lambda for creating lacZ protein fusions in a single step. J Bacteriol 1984 2.52
38 Function of conserved histidine-243 in phosphatase activity of EnvZ, the sensor for porin osmoregulation in Escherichia coli. J Bacteriol 1997 2.48
39 CpxP, a stress-combative member of the Cpx regulon. J Bacteriol 1998 2.42
40 Mutations altering the cellular localization of the phage lambda receptor, an Escherichia coli outer membrane protein. Proc Natl Acad Sci U S A 1978 2.37
41 Identification of OmpR: a positive regulatory protein controlling expression of the major outer membrane matrix porin proteins of Escherichia coli K-12. J Bacteriol 1981 2.36
42 Genetic evidence for parallel pathways of chaperone activity in the periplasm of Escherichia coli. J Bacteriol 2001 2.33
43 Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export. J Biol Chem 1986 2.33
44 The Cpx envelope stress response is controlled by amplification and feedback inhibition. J Bacteriol 1999 2.26
45 Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli. Annu Rev Genet 1998 2.20
46 The sigmaE and Cpx regulatory pathways: overlapping but distinct envelope stress responses. Curr Opin Microbiol 1999 2.20
47 EnvZ functions through OmpR to control porin gene expression in Escherichia coli K-12. J Bacteriol 1988 2.20
48 Sequence information within the lamB genes in required for proper routing of the bacteriophage lambda receptor protein to the outer membrane of Escherichia coli K-12. J Mol Biol 1982 2.20
49 Mutations that alter the kinase and phosphatase activities of the two-component sensor EnvZ. J Bacteriol 1998 2.19
50 Cpx signaling pathway monitors biogenesis and affects assembly and expression of P pili. EMBO J 2001 2.08
51 The sec and prl genes of Escherichia coli. J Bioenerg Biomembr 1990 2.07
52 Heat-shock proteins DnaK and GroEL facilitate export of LacZ hybrid proteins in E. coli. Nature 1990 2.05
53 A signal sequence is not sufficient to lead beta-galactosidase out of the cytoplasm. Nature 1980 2.00
54 PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli. Cell 1990 1.99
55 Isolation and characterization of delta ompB strains of Escherichia coli by a general method based on gene fusions. J Bacteriol 1985 1.99
56 Importance of secondary structure in the signal sequence for protein secretion. Proc Natl Acad Sci U S A 1983 1.98
57 Isolation and characterization of mutations altering expression of the major outer membrane porin proteins using the local anaesthetic procaine. J Mol Biol 1983 1.97
58 Information within the mature LamB protein necessary for localization to the outer membrane of E coli K12. Cell 1983 1.94
59 A genetic approach for analyzing the pathway of LamB assembly into the outer membrane of Escherichia coli. J Biol Chem 1991 1.92
60 cis-acting sites required for osmoregulation of ompF expression in Escherichia coli K-12. J Bacteriol 1986 1.90
61 D-ribose metabolism in Escherichia coli K-12: genetics, regulation, and transport. J Bacteriol 1984 1.89
62 One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery. EMBO J 1991 1.89
63 Genetic analysis of protein export in Escherichia coli. Methods Enzymol 1983 1.88
64 Genetic analysis of the switch that controls porin gene expression in Escherichia coli K-12. J Mol Biol 1989 1.85
65 The essential tension: opposed reactions in bacterial two-component regulatory systems. Trends Microbiol 1993 1.85
66 On the significance of the retention of ligand by protein. Proc Natl Acad Sci U S A 1975 1.83
67 Identification of the malK gene product. A peripheral membrane component of the Escherichia coli maltose transport system. J Biol Chem 1981 1.80
68 Suppressor mutations in rpoA suggest that OmpR controls transcription by direct interaction with the alpha subunit of RNA polymerase. J Bacteriol 1991 1.77
69 Genetic basis for activity differences between vancomycin and glycolipid derivatives of vancomycin. Science 2001 1.77
70 PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains. EMBO J 1993 1.77
71 Characterization and in vivo cloning of prlC, a suppressor of signal sequence mutations in Escherichia coli K12. Genetics 1987 1.74
72 Intragenic regions required for LamB export. Proc Natl Acad Sci U S A 1984 1.74
73 Mutational activation of the Cpx signal transduction pathway of Escherichia coli suppresses the toxicity conferred by certain envelope-associated stresses. Mol Microbiol 1995 1.70
74 Mutations that affect separate functions of OmpR the phosphorylated regulator of porin transcription in Escherichia coli. J Mol Biol 1993 1.65
75 Molecular components of the signal sequence that function in the initiation of protein export. J Cell Biol 1982 1.65
76 Crl stimulates RpoS activity during stationary phase. Mol Microbiol 1998 1.60
77 Beta-galactosidase is inactivated by intermolecular disulfide bonds and is toxic when secreted to the periplasm of Escherichia coli. J Bacteriol 1995 1.56
78 Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking. J Bacteriol 1999 1.52
79 New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli. Genes Dev 1989 1.50
80 Periplasmic protein related to the sn-glycerol-3-phosphate transport system of Escherichia coli. J Bacteriol 1976 1.49
81 Suppressor analysis suggests a multistep, cyclic mechanism for protein secretion in Escherichia coli. EMBO J 1992 1.46
82 Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules. J Bacteriol 1979 1.44
83 Isolation of mutations in the alpha operon of Escherichia coli that suppress the transcriptional defect conferred by a mutation in the porin regulatory gene envZ. J Bacteriol 1987 1.44
84 OmpR mutants specifically defective for transcriptional activation. J Mol Biol 1994 1.44
85 Accumulation of the enterobacterial common antigen lipid II biosynthetic intermediate stimulates degP transcription in Escherichia coli. J Bacteriol 1998 1.43
86 Tethering of CpxP to the inner membrane prevents spheroplast induction of the cpx envelope stress response. Mol Microbiol 2000 1.42
87 Mutations that define the promoter of ompF, a gene specifying a major outer membrane porin protein. J Bacteriol 1985 1.38
88 A second transport system for sn-glycerol-3-phosphate in Escherichia coli. J Bacteriol 1978 1.34
89 Phosphorylation-dependent conformational changes in OmpR, an osmoregulatory DNA-binding protein of Escherichia coli. Proc Natl Acad Sci U S A 1995 1.33
90 PrlA4 prevents the rejection of signal sequence defective preproteins by stabilizing the SecA-SecY interaction during the initiation of translocation. EMBO J 1998 1.32
91 Alpha: the Cinderella subunit of RNA polymerase. J Biol Chem 1992 1.31
92 Signal transduction in bacteria: kinases that control gene expression. New Biol 1990 1.29
93 Genetic fusions as experimental tools. Methods Enzymol 1991 1.28
94 PrlA and PrlG suppressors reduce the requirement for signal sequence recognition. J Bacteriol 1994 1.26
95 PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli. Proc Natl Acad Sci U S A 1989 1.24
96 Chimeric genetics with beta-galactosidase. Gene Amplif Anal 1983 1.24
97 Sequence information required for protein translocation from the cytoplasm. J Bacteriol 1987 1.19
98 The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE. EMBO J 1995 1.18
99 Signal sequence processing is required for the assembly of LamB trimers in the outer membrane of Escherichia coli. J Bacteriol 1993 1.18
100 Transposition of lambda placMu is mediated by the A protein altered at its carboxy-terminal end. Gene 1988 1.18
101 The LysR homolog LrhA promotes RpoS degradation by modulating activity of the response regulator sprE. J Bacteriol 1999 1.17
102 Mutation prlF1 relieves the lethality associated with export of beta-galactosidase hybrid proteins in Escherichia coli. J Bacteriol 1984 1.17
103 Identification of base pairs important for OmpR-DNA interaction. Mol Microbiol 1995 1.16
104 Two-dimensional polyacylamide gel electrophoresis of envelope proteins of Escherichia coli. Appl Microbiol 1975 1.14
105 Isolation and characterization of mutants of Escherichia coli K12 affected in protein localization. Methods Enzymol 1983 1.12
106 Two cellular components, PrlA and SecB, that recognize different sequence determinants are required for efficient protein export. J Bacteriol 1988 1.11
107 RpoS-dependent transcriptional control of sprE: regulatory feedback loop. J Bacteriol 2001 1.10
108 Enhanced export of beta-galactosidase fusion proteins in prlF mutants is Lon dependent. J Bacteriol 1992 1.10
109 Escherichia coli xonA (sbcB) mutants enhance illegitimate recombination. Genetics 1991 1.09
110 Escherichia coli prlC encodes an endopeptidase and is homologous to the Salmonella typhimurium opdA gene. J Bacteriol 1992 1.09
111 Conferral of transposable properties to a chromosomal gene in Escherichia coli. J Mol Biol 1980 1.08
112 Selection for mutants altered in the expression or export of outer membrane porin OmpF. J Bacteriol 1985 1.08
113 The first 28 amino acids of mature LamB are required for rapid and efficient export from the cytoplasm. Genes Dev 1987 1.07
114 PrlC, a suppressor of signal sequence mutations in Escherichia coli, can direct the insertion of the signal sequence into the membrane. J Mol Biol 1989 1.07
115 Suppression of signal sequence defects and azide resistance in Escherichia coli commonly result from the same mutations in secA. J Bacteriol 1995 1.04
116 The genetics of protein secretion in Escherichia coli. Methods Cell Biol 1981 1.02
117 A progenitor of the outer membrane LamB trimer. J Bacteriol 1988 1.01
118 A practical guide to the construction and use of lac fusions in Escherichia coli. Methods Enzymol 2000 0.99
119 Increased expression of the bifunctional protein PrlF suppresses overproduction lethality associated with exported beta-galactosidase hybrid proteins in Escherichia coli. J Bacteriol 1990 0.98
120 Absence of the outer membrane phospholipase A suppresses the temperature-sensitive phenotype of Escherichia coli degP mutants and induces the Cpx and sigma(E) extracytoplasmic stress responses. J Bacteriol 2001 0.96
121 Resolution of glpA and glpT loci into separate operons in Escherichia coli K-12 strains. J Bacteriol 1979 0.96
122 The genetics of protein secretion in E. coli. Trends Genet 1990 0.94
123 Engineering Escherichia coli to secrete heterologous gene products. Methods Enzymol 1990 0.92
124 The genetics of protein targeting in Escherichia coli K12. J Cell Sci Suppl 1989 0.91
125 Multicopy suppression of cold-sensitive sec mutations in Escherichia coli. J Bacteriol 1995 0.87
126 SprE levels are growth phase regulated in a sigma(S)-dependent manner at the level of translation. J Bacteriol 2000 0.87
127 Genetic studies on mechanisms of protein localization in Escherichia coli K-12. J Supramol Struct 1980 0.85
128 Folding-based suppression of extracytoplasmic toxicity conferred by processing-defective LamB. J Bacteriol 1998 0.84
129 Gene fusions to the ptsM/pel locus of Escherichia coli. Mol Gen Genet 1985 0.83
130 Involvement of a tryptophan residue in the binding site of Escherichia coli galactose-binding protein. Biochemistry 1974 0.83
131 Selection procedure for mutants defective in the beta-methylgalactoside transport system of Escherichia coli utilizing the compound 2R-glyceryl-beta-D-galactopyranoside. J Bacteriol 1974 0.83
132 BLAST 1995: international conference on bacterial locomotion and signal transduction. Mol Microbiol 1995 0.83
133 Membrane-derived oligosaccharides affect porin osmoregulation only in media of low ionic strength. J Bacteriol 1992 0.82
134 lacZ fusions to genes that specify exported proteins: a general technique. Mol Gen Genet 1984 0.82
135 The signal hypothesis in bacteria. Prog Clin Biol Res 1982 0.80
136 A convenient synthesis of (2R)-glyceryl-beta-D-galactopyranoside. A substrate for beta-galactosidase, the lactose repressor, the galactose-binding protein, and the beta-methylgalactoside transport system. J Biol Chem 1973 0.79
137 The "hidden ligand" of the galactose-binding protein. Eur J Biochem 1975 0.77
138 Synthesis of l-(p-iodobenzenesulfonyl)-3,5-di-n-propyl isocyanurate. J Org Chem 1972 0.77
139 Cell regulation: continually redefining the rules. Curr Opin Microbiol 1998 0.75
140 Synthesis and pharmacological activity of 1-(arylsulfonyl)-3,5-dialkyl-s-triazine-2,4,6-(1H,3H,5H)-triones. J Pharm Sci 1973 0.75