Published in Biochemistry on January 11, 2000
Testing electrostatic complementarity in enzyme catalysis: hydrogen bonding in the ketosteroid isomerase oxyanion hole. PLoS Biol (2006) 2.41
Decomposition of vibrational shifts of nitriles into electrostatic and hydrogen-bonding effects. J Am Chem Soc (2010) 1.46
Ketosteroid isomerase provides further support for the idea that enzymes work by electrostatic preorganization. Proc Natl Acad Sci U S A (2010) 1.23
Hydrogen bond coupling in the ketosteroid isomerase active site. Biochemistry (2009) 1.02
Proton affinity of the oxyanion hole in the active site of ketosteroid isomerase. Biochemistry (2010) 0.98
Quantitative dissection of hydrogen bond-mediated proton transfer in the ketosteroid isomerase active site. Proc Natl Acad Sci U S A (2013) 0.91
Hydrogen bonding in the active site of ketosteroid isomerase: electronic inductive effects and hydrogen bond coupling. Biochemistry (2010) 0.88
Experimental and computational mutagenesis to investigate the positioning of a general base within an enzyme active site. Biochemistry (2014) 0.82
Water in the active site of ketosteroid isomerase. Biochemistry (2011) 0.81
Solution structure of 3-oxo-delta5-steroid isomerase. Science (1997) 1.27
Energetics of 3-oxo-delta 5-steroid isomerase: source of the catalytic power of the enzyme. Biochemistry (1991) 1.24
Evaluation of the internal equilibrium constant for 3-oxo-delta 5-steroid isomerase using the D38E and D38N mutants: the energetic basis for catalysis. Biochemistry (1994) 1.15
Nature of the intermediate in the 3-oxo-delta 5-steroid isomerase reaction. Biochemistry (1992) 1.00
Substituent effects on the binding of phenols to the D38N mutant of 3-oxo-delta5-steroid isomerase. A probe for the nature of hydrogen bonding to the intermediate. Biochemistry (1998) 0.98
pH dependence of the kinetic parameters for 3-oxo-delta 5-steroid isomerase. Substrate catalysis and inhibition by (3S)-spiro[5 alpha-androstane-3,2'-oxiran]-17-one. Biochemistry (1986) 0.95
Electrophilic assistance by Asp-99 of 3-oxo-Delta 5-steroid isomerase. Biochemistry (1998) 0.91
Kinetic competence of an externally generated dienol intermediate with steroid isomerase. Biochemistry (1991) 0.89
Selective catalysis of elementary steps by Asp-99 and Tyr-14 of 3-Oxo-delta(5)-steroid isomerase. J Am Chem Soc (2001) 0.84
An active-site-directed irreversible inhibitor of delta 5-3-ketosteroid isomerase. Biochem Biophys Res Commun (1979) 0.83
Influence of Leaving-Group Electronic Effect on alpha-Chymotrypsin: Catalytic Constants of Specific Substrates. Proc Natl Acad Sci U S A (1973) 0.82
Catalytic activity of the D38A mutant of 3-oxo-Delta 5-steroid isomerase: recruitment of aspartate-99 as the base. Biochemistry (2000) 0.81
Letter: Primary amine cytalysis of the isomerization of a beta, gamma-unsatureate ketone to its alpha, beta-unsaturated isomer. A possible model for enzymatic double bone migration in unsaturated ketones. J Am Chem Soc (1975) 0.81
Affinity alkylation of 3-oxo-delta 5-steroid isomerase by steroidal 3 beta-oxiranes: identification of the modified amino acid by reduction with hydroxyborohydride. Biochemistry (1987) 0.81
Catalytic contribution of phenylalanine-101 of 3-oxo-Delta 5-steroid isomerase. Biochemistry (1998) 0.78
An active site phenylalanine of 3-oxo-delta 5-steroid isomerase is catalytically important for proton transfer. Biochemistry (1995) 0.78
Affinity alkylation of bacterial delta 5-3-ketosteroid isomerase. Identification of the amino acid modified by steroidal 17 beta-oxiranes. J Biol Chem (1983) 0.78
Mechanism of proton transfer in the isomerization of 5-androstene-3, 17-dione by 3-oxo-delta 5-steroid isomerase and its D38E mutant. Biochemistry (1996) 0.77
Detection of a transient enzyme-steroid complex during active-site-directed irreversible inhibition of 3-oxo-delta 5-steroid isomerase. Biochemistry (1986) 0.77
Is a proton relay involved in the mechanism of 3-oxo-delta 5-steroid isomerase? Biochemistry (1993) 0.77
Orientation, accessibility, and mobility of equilenin bound to the active site of steroid isomerase. Biochemistry (1989) 0.77
Reaction energetics of a mutant 3-oxo-delta 5-steroid isomerase with an altered active site base (D38E). Biochemistry (1994) 0.77
Effect of membrane lipid environment on the activity of bovine adrenal 3-oxo-delta 5-steroid isomerase. Steroids (1987) 0.75
On the use of p-nitroanilides as substrates for proteolytic enzymes. FEBS Lett (1974) 0.75
Irreversible active-site-directed inhibition of delta 5-3-ketosteroid isomerase by steroidal 17-beta-oxiranes. Evidence for two modes of binding in steroid-enzyme complexes. Biochem Biophys Res Commun (1980) 0.75
Mechanism of inactivation of 3-oxosteroid delta 5-isomerase by 17 beta-oxiranes. Biochemistry (1985) 0.75