PubRank

R R Burgess

Author PubWeight™ 249.08‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography. Biochemistry 1975 16.43
2 Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes. Anal Biochem 1980 14.14
3 A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase. J Biol Chem 1969 12.49
4 Factor stimulating transcription by RNA polymerase. Nature 1969 9.27
5 Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins. Nucleic Acids Res 1986 7.28
6 The codon preference plot: graphic analysis of protein coding sequences and prediction of gene expression. Nucleic Acids Res 1984 6.98
7 Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase. Biochemistry 1979 5.75
8 The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12. Cell 1983 5.73
9 The nucleotide sequence of the cloned rpoD gene for the RNA polymerase sigma subunit from E coli K12. Nucleic Acids Res 1981 5.52
10 Architecture of RNA polymerase II and implications for the transcription mechanism. Science 2000 5.12
11 Separation and characterization of the subunits of ribonucleic acid polymerase. J Biol Chem 1969 4.98
12 Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II. J Biol Chem 1989 3.90
13 Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase. Cell 1984 3.10
14 Transcription of a gene for human U1 small nuclear RNA. Cell 1982 2.93
15 RNA polymerase. Annu Rev Biochem 1971 2.54
16 Synthesis of human U1 RNA. II. Identification of two regions of the promoter essential for transcription initiation at position +1. J Biol Chem 1984 2.46
17 Identification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome. Proc Natl Acad Sci U S A 1975 2.45
18 Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody. J Biol Chem 1990 2.45
19 Replacement of potassium chloride by potassium glutamate dramatically enhances protein-DNA interactions in vitro. Biochemistry 1987 2.45
20 Characterization of the Escherichia coli transcription factor sigma 70: localization of a region involved in the interaction with core RNA polymerase. Biochemistry 1989 2.40
21 Nucleotide sequence preference at rat liver and wheat germ type 1 DNA topoisomerase breakage sites in duplex SV40 DNA. Nucleic Acids Res 1984 2.37
22 Use of Mono Q high-resolution ion-exchange chromatography to obtain highly pure and active Escherichia coli RNA polymerase. Biochemistry 1990 2.35
23 Stringent response in Escherichia coli induces expression of heat shock proteins. J Mol Biol 1985 2.26
24 In vitro functional characterization of overproduced Escherichia coli katF/rpoS gene product. Biochemistry 1993 2.16
25 A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II. Biochemistry 1975 2.14
26 Promoter recognition and discrimination by EsigmaS RNA polymerase. Mol Microbiol 2001 2.06
27 Chlamydia trachomatis RNA polymerase major sigma subunit. Sequence and structural comparison of conserved and unique regions with Escherichia coli sigma 70 and Bacillus subtilis sigma 43. J Biol Chem 1990 2.04
28 Purification and characterization of proximal sequence element-binding protein 1, a transcription activating protein related to Ku and TREF that binds the proximal sequence element of the human U1 promoter. J Biol Chem 1990 2.03
29 Termination efficiency at rho-dependent terminators depends on kinetic coupling between RNA polymerase and rho. Proc Natl Acad Sci U S A 1992 2.01
30 RNA polymerases from Bacillus subtilis and Escherichia coli differ in recognition of regulatory signals in vitro. J Bacteriol 2000 1.98
31 Temperature dependence of the rate constants of the Escherichia coli RNA polymerase-lambda PR promoter interaction. Assignment of the kinetic steps corresponding to protein conformational change and DNA opening. J Mol Biol 1985 1.93
32 Overexpression and purification of the sigma subunit of Escherichia coli RNA polymerase. Gene 1983 1.92
33 Functional elements of the human U1 RNA promoter. Identification of five separate regions required for efficient transcription and template competition. J Biol Chem 1987 1.91
34 Yeast RNA polymerase II at 5 A resolution. Cell 1999 1.90
35 Structure and function of the sigma-70 subunit of Escherichia coli RNA polymerase. Monoclonal antibodies: localization of epitopes by peptide mapping and effects on transcription. Biochemistry 1988 1.87
36 Kinetics and mechanism of the interaction of Escherichia coli RNA polymerase with the lambda PR promoter. J Mol Biol 1984 1.84
37 Tagetitoxin: a new inhibitor of eukaryotic transcription by RNA polymerase III. J Biol Chem 1990 1.77
38 Inhibition of RNA polymerase by streptolydigin. Nat New Biol 1971 1.76
39 An RNA polymerase mutant with reduced accuracy of chain elongation. Biochemistry 1986 1.75
40 Mutations in the Ion gene of E. coli K12 phenotypically suppress a mutation in the sigma subunit of RNA polymerase. Cell 1983 1.74
41 Nonspecific interactions of Escherichia coli RNA polymerase with native and denatured DNA: differences in the binding behavior of core and holoenzyme. Biochemistry 1978 1.67
42 Cloning and in vivo and in vitro regulation of cyclic AMP-dependent carbon starvation genes from Escherichia coli. J Bacteriol 1990 1.61
43 Localization of a sigma70 binding site on the N terminus of the Escherichia coli RNA polymerase beta' subunit. J Biol Chem 1998 1.60
44 Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies. J Biol Chem 1991 1.58
45 Effects of the mutant sigma allele rpoD800 on the synthesis of specific macromolecular components of the Escherichia coli K12 cell. J Mol Biol 1984 1.55
46 A coiled-coil from the RNA polymerase beta' subunit allosterically induces selective nontemplate strand binding by sigma(70). Cell 2001 1.55
47 Manganese, Mn-dependent peroxidases, and the biodegradation of lignin. Biochem Biophys Res Commun 1988 1.54
48 Purification and characterization of wheat germ DNA topoisomerase I (nicking-closing enzyme). J Biol Chem 1981 1.49
49 A novel collection of accessory factors associated with yeast RNA polymerase II. Protein Expr Purif 1996 1.49
50 Escherichia coli RNA polymerase binding and initiation of transcription on fragments of lambda rifd 18 DNA containing promoters for lambda genes and for rrnB, tufB, rplC,A, rplJ,L, and rpoB,C genes. Gene 1979 1.46
51 The HIP1 initiator element plays a role in determining the in vitro requirement of the dihydrofolate reductase gene promoter for the C-terminal domain of RNA polymerase II. Mol Cell Biol 1992 1.35
52 Rapid response to osmotic upshift by osmoregulated genes in Escherichia coli and Salmonella typhimurium. J Bacteriol 1988 1.34
53 Overproduction and purification of sigma 32, the Escherichia coli heat shock transcription factor. Protein Expr Purif 1993 1.33
54 Use of polyethyleneimine in purification of DNA-binding proteins. Methods Enzymol 1991 1.33
55 Purification and lipid-layer crystallization of yeast RNA polymerase II. Proc Natl Acad Sci U S A 1990 1.31
56 Construction of bacteriophage T7 late promoters with point mutations and characterization by in vitro transcription properties. Nucleic Acids Res 1987 1.31
57 Nucleotide sequence of the rpsU-dnaG-rpoD operon from Salmonella typhimurium and a comparison of this sequence with the homologous operon of Escherichia coli. Gene 1985 1.28
58 The human U1 snRNA promoter correctly initiates transcription in vitro and is activated by PSE1. Genes Dev 1990 1.28
59 In an Escherichia coli coupled transcription-translation system, expression of the osmoregulated gene proU is stimulated at elevated potassium concentrations and by an extract from cells grown at high osmolality. J Biol Chem 1989 1.28
60 PEPPLOT, a protein secondary structure analysis program for the UWGCG sequence analysis software package. Nucleic Acids Res 1986 1.27
61 Tagetitoxin inhibition of RNA polymerase III transcription results from enhanced pausing at discrete sites and is template-dependent. J Biol Chem 1992 1.25
62 Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli RNA polymerase. Biochemistry 1992 1.25
63 Characterization of a rho-dependent termination site within the cro gene of bacteriophage lambda. J Biol Chem 1980 1.24
64 Studies of the role of the Escherichia coli heat shock regulatory protein sigma 32 by the use of monoclonal antibodies. J Biol Chem 1987 1.23
65 Rapid purification of His(6)-tagged Bacillus subtilis core RNA polymerase. Protein Expr Purif 2000 1.22
66 Immune response in the mouse to endogenous leukemia viruses. Cold Spring Harb Symp Quant Biol 1975 1.22
67 Variables affecting the selectivity and efficiency of retention of DNA fragments by E. coli RNA polymerase in the nitrocellulose-filter-binding assay. Gene 1981 1.21
68 Characteristics and N-terminal amino acid sequence of a manganese peroxidase purified from Lentinula edodes cultures grown on a commercial wood substrate. Appl Microbiol Biotechnol 1990 1.21
69 Interactions of T7 RNA polymerase with T7 late promoters measured by footprinting with methidiumpropyl-EDTA-iron(II). Biochemistry 1987 1.15
70 Nitrocellulose filter binding studies of the interactions of Escherichia coli RNA polymerase holoenzyme with deoxyribonucleic acid restriction fragments: evidence for multiple classes of nonpromoter interactions, some of which display promoter-like properties. Biochemistry 1982 1.14
71 rpoZ, encoding the omega subunit of Escherichia coli RNA polymerase, is in the same operon as spoT. J Bacteriol 1989 1.14
72 Mutational analysis of beta '260-309, a sigma 70 binding site located on Escherichia coli core RNA polymerase. J Biol Chem 2000 1.13
73 Direct evidence for the preferential binding of Escherichia coli RNA polymerase holoenzyme to the ends of deoxyribonucleic acid restriction fragments. Biochemistry 1983 1.11
74 The cloning and sequence of the gene encoding the omega subunit of Escherichia coli RNA polymerase. Gene 1986 1.09
75 Bacteriophage T7 late promoters with point mutations: quantitative footprinting and in vivo expression. Nucleic Acids Res 1988 1.06
76 Transcription of simian virus 40 DNA by wheat germ RNA polymerase II. Priming of RNA synthesis by the 3'-hydroxyl of DNA at single strand nicks. J Biol Chem 1980 1.06
77 The leucine-responsive regulatory protein (Lrp) acts as a specific repressor for sigma s-dependent transcription of the Escherichia coli aidB gene. Mol Microbiol 1996 1.05
78 Measurement of binding constants for protein-DNA interactions by DNA-cellulose chromatography. Biochemistry 1977 1.03
79 Binding of the initiation factor sigma(70) to core RNA polymerase is a multistep process. Mol Cell 2001 1.03
80 In vitro thermal inactivation of a temperature-sensitive sigma subunit mutant (rpoD800) of Escherichia coli RNA polymerase proceeds by aggregation. J Biol Chem 1981 1.02
81 In vitro use of monoclonal antibodies in Escherichia coli S-30 extracts to determine the RNA polymerase sigma subunit required by a promoter. J Biol Chem 1989 1.01
82 Ion effects on the aggregation and DNA-binding reactions of Escherichia coli RNA polymerase. Cold Spring Harb Symp Quant Biol 1983 1.00
83 Studies of the subunit structure of wheat germ ribonucleic acid polymerase II. Biochemistry 1977 1.00
84 Binding of Escherichia coli ribonucleic acid polymerase holoenzyme to a bacteriophage T7 promoter-containing fragment: selectivity exists over a wide range of solution conditions. Biochemistry 1980 1.00
85 Isolation and characterization of transducing phage coding for sigma subunit of Escherichia coli RNA polymerase. Proc Natl Acad Sci U S A 1979 1.00
86 Binding of Escherichia coli ribonucleic acid polymerase holoenzyme to a bacteriophage T7 promoter-containing fragment: evaluation of promoter binding constants as a function of solution conditions. Biochemistry 1980 1.00
87 Escherichia coli RNA polymerase: purification, subunit structure, and factor requirements. Fed Proc 1970 1.00
88 In vitro transcription by wheat germ ribonucleic acid polymerase II: effects of heparin and role of template integrity. Biochemistry 1979 0.99
89 Identification of a heat shock promoter in the topA gene of Escherichia coli. J Bacteriol 1990 0.98
90 Overproduction and purification of the omega subunit of Escherichia coli RNA polymerase. Protein Expr Purif 1990 0.97
91 Binding of transcription factors to the promoter of the human U1 RNA gene studied by footprinting. J Biol Chem 1988 0.97
92 Cysteine and methionine content of the Escherichia coli ribonucleic acid polymerase subunits. J Bacteriol 1976 0.96
93 Immunoaffinity purification of RNA polymerase II and transcription factors using polyol-responsive monoclonal antibodies. Methods Enzymol 1996 0.96
94 Aggregation equilibria of Escherichia coli RNA polymerase: evidence for anion-linked conformational transitions in the protomers of core and holoenzyme. Biochemistry 1982 0.95
95 Epitope mapping using histidine-tagged protein fragments: application to Escherichia coli RNA polymerase sigma 70. Anal Biochem 1996 0.93
96 Cross-linking of Escherichia coli RNA polymerase subunits: identification of beta' as the binding site of omega. Biochemistry 1993 0.91
97 Altered chemical properties in three mutants of E. coli RNA polymerase sigma subunit. Mol Gen Genet 1979 0.91
98 Analysis of the in vitro synthesis of 5'-gamma-32P-labeled transcripts from coliphage lambda by gel electrophoresis, RNA-DNA hybridization, and RNase T1 digestion. J Biol Chem 1980 0.90
99 Selectivity of RNA chain initiation in vitro. 1. Analysis of RNA initiations by two-dimensional thin-layer chromatography of 5'-triphosphate-labeled oligonucleotides. Biochemistry 1978 0.90
100 In Vitro Synthesis and Processing of Wheat alpha-Amylase : TRANSLATION OF GIBBERELLIC ACID-INDUCED WHEAT ALEURONE LAYER RNA BY WHEAT GERM AND XENOPUS LAEVIS OOCYTE SYSTEMS. Plant Physiol 1982 0.90
101 Purification of Xenopus egg nucleoplasmin and its use in chromatin assembly in vitro. Methods Enzymol 1989 0.90
102 An overview of a feasibility study for the production of industrial enzymes in transgenic alfalfa. Ann N Y Acad Sci 1994 0.90
103 Comparative analysis of the interactions of Escherichia coli sigma S and sigma 70 RNA polymerase holoenzyme with the stationary-phase-specific bolAp1 promoter. Biochemistry 1997 0.88
104 In vitro transcription from the b2 region of bacteriophage lambda. Virology 1980 0.88
105 Biosynthesis of Escherichia coli RNA polymerase subunits upon release of rifampicin inhibition. Mol Gen Genet 1976 0.87
106 In vitro transcription by wheat germ RNA polymerase II. Initiation of RNA synthesis on relaxed, closed circular template. J Biol Chem 1981 0.87
107 DNA strand breakage by wheat germ type 1 topoisomerase. Biochim Biophys Acta 1984 0.87
108 Use of aryl azide cross-linkers to investigate protein-protein interactions: an optimization of important conditions as applied to Escherichia coli RNA polymerase and localization of a sigma 70-alpha cross-link to the C-terminal region of alpha. Biochemistry 1994 0.87
109 Identification of polyol-responsive monoclonal antibodies for use in immunoaffinity chromatography. Curr Protoc Mol Biol 2001 0.86
110 Use of difference boundary sedimentation velocity to investigate nonspecific protein-nucleic acid interactions. Biochemistry 1980 0.85
111 Rpb3, stoichiometry and sequence determinants of the assembly into yeast RNA polymerase II in vivo. J Biol Chem 1998 0.84
112 Templates for eukaryotic RNA polymerase II: artefacts can produce an apparent preference for denatured DNA over native DNA. Anal Biochem 1977 0.83
113 Single-step synthesis and characterization of biotinylated nitrilotriacetic acid, a unique reagent for the detection of histidine-tagged proteins immobilized on nitrocellulose. Anal Biochem 1996 0.83
114 Use of asymmetric PCR to generate long primers and single-stranded DNA for incorporating cross-linking analogs into specific sites in a DNA probe. Genome Res 1996 0.82
115 Identification of the epitope for a highly cross-reactive monoclonal antibody on the major sigma factor of bacterial RNA polymerase. J Bacteriol 1997 0.81
116 Immobilization of manganese peroxidase from Lentinula edodes and its biocatalytic generation of MnIII-chelate as a chemical oxidant of chlorophenols. Biotechnol Bioeng 1998 0.81
117 Accessibility of epitopes on human transcription factor IIB in the native protein and in a complex with DNA. J Biol Chem 1995 0.81
118 RNA polymerase II from wheat germ contains tightly bound zinc. Biochem Biophys Res Commun 1977 0.80
119 Mapping protease susceptibility sites on the Escherichia coli transcription factor sigma70. Biochemistry 1999 0.80
120 Immobilization of manganese peroxidase from Lentinula edodes on alkylaminated Emphaze AB 1 polymer for generation of Mn3+ as an oxidizing agent. Appl Biochem Biotechnol 1996 0.79
121 Nuclear phosphoproteins of Physarum polycephalum. Characterization and phosphorus content of the phenol-soluble nuclear acidic proteins. Arch Biochem Biophys 1975 0.79
122 Purification of recombinant human transcription factor IIB by immunoaffinity chromatography. Protein Expr Purif 1994 0.78
123 Overproduction of Escherichia coli NusA protein. Gene 1983 0.78
124 Overproduction and purification of sigmaS, the Escherichia coli stationary phase specific sigma transcription factor. Protein Expr Purif 1996 0.78
125 Immunoaffinity purification of the RAP30 subunit of human transcription factor IIF. Protein Expr Purif 1999 0.76
126 Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking. Biochemistry 2000 0.76
127 Purification and properties of two RNA polymerases from Physarum polycephalum. Proc Natl Acad Sci U S A 1974 0.75
128 Regulation of cyclic AMP synthesis in Escherichia coli K-12: effects of the rpoD800 sigma mutation, glucose, and chloramphenicol. J Bacteriol 1984 0.75
129 Selectivity of RNA chain initiation in vitro. 3. Variables affecting initiation of transcription. Biochemistry 1978 0.75
130 Selectivity of RNA chain initiation in vitro. 2. Correlation of 5'-triphosphate-labeled oligonucleotides on polyethyleniminecellulose thin-layer chromatography with RNA transcripts of bacteriophage lambda cb2 and T7. Biochemistry 1978 0.75