Published in Biochemistry on July 27, 2001
Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme. Science (2004) 2.65
Anaerobic functionalization of unactivated C-H bonds. Curr Opin Chem Biol (2009) 1.71
Radical S-adenosylmethionine enzymes. Chem Rev (2014) 1.64
RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA. J Am Chem Soc (2010) 1.60
Self-sacrifice in radical S-adenosylmethionine proteins. Curr Opin Chem Biol (2007) 1.55
In vitro characterization of AtsB, a radical SAM formylglycine-generating enzyme that contains three [4Fe-4S] clusters. Biochemistry (2008) 1.46
Biotin synthase contains two distinct iron-sulfur cluster binding sites: chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions. Biochemistry (2001) 1.43
Control of radical chemistry in the AdoMet radical enzymes. Curr Opin Chem Biol (2009) 1.34
Characterization of RimO, a new member of the methylthiotransferase subclass of the radical SAM superfamily. Biochemistry (2009) 1.33
Synthesis of the 2Fe subcluster of the [FeFe]-hydrogenase H cluster on the HydF scaffold. Proc Natl Acad Sci U S A (2010) 1.26
SufA/IscA: reactivity studies of a class of scaffold proteins involved in [Fe-S] cluster assembly. J Biol Inorg Chem (2004) 1.21
Adenosyl radical: reagent and catalyst in enzyme reactions. Chembiochem (2010) 1.19
Control of adenosylmethionine-dependent radical generation in biotin synthase: a kinetic and thermodynamic analysis of substrate binding to active and inactive forms of BioB. Biochemistry (2003) 1.15
Characterization and mechanistic studies of DesII: a radical S-adenosyl-L-methionine enzyme involved in the biosynthesis of TDP-D-desosamine. J Am Chem Soc (2009) 1.15
The sufR gene (sll0088 in Synechocystis sp. strain PCC 6803) functions as a repressor of the sufBCDS operon in iron-sulfur cluster biogenesis in cyanobacteria. J Bacteriol (2004) 1.09
Two Fe-S clusters catalyze sulfur insertion by radical-SAM methylthiotransferases. Nat Chem Biol (2013) 1.08
In vitro activation of [FeFe] hydrogenase: new insights into hydrogenase maturation. J Biol Inorg Chem (2007) 1.07
Evidence from Mössbauer spectroscopy for distinct [2Fe-2S](2+) and [4Fe-4S](2+) cluster binding sites in biotin synthase from Escherichia coli. J Am Chem Soc (2002) 1.04
SPASM and twitch domains in S-adenosylmethionine (SAM) radical enzymes. J Biol Chem (2014) 1.01
9-Mercaptodethiobiotin is formed as a competent catalytic intermediate by Escherichia coli biotin synthase. Biochemistry (2008) 0.95
Overexpression of biotin synthase and biotin ligase is required for efficient generation of sulfur-35 labeled biotin in E. coli. BMC Biotechnol (2010) 0.95
The biosynthesis of thiol- and thioether-containing cofactors and secondary metabolites catalyzed by radical S-adenosylmethionine enzymes. J Biol Chem (2014) 0.93
Role of the [2Fe-2S]2+ cluster in biotin synthase: mutagenesis of the atypical metal ligand arginine 260. Biochemistry (2006) 0.92
9-Mercaptodethiobiotin is generated as a ligand to the [2Fe-2S]+ cluster during the reaction catalyzed by biotin synthase from Escherichia coli. J Am Chem Soc (2012) 0.89
Covalent intermediate in the catalytic mechanism of the radical S-adenosyl-L-methionine methyl synthase RlmN trapped by mutagenesis. J Am Chem Soc (2012) 0.87
Reduction of the [2Fe-2S] cluster accompanies formation of the intermediate 9-mercaptodethiobiotin in Escherichia coli biotin synthase. Biochemistry (2011) 0.85
Loss of iron-sulfur clusters from biotin synthase as a result of catalysis promotes unfolding and degradation. Arch Biochem Biophys (2007) 0.85
Evidence for a catalytically and kinetically competent enzyme-substrate cross-linked intermediate in catalysis by lipoyl synthase. Biochemistry (2014) 0.85
A complex between biotin synthase and the iron-sulfur cluster assembly chaperone HscA that enhances in vivo cluster assembly. Biochemistry (2009) 0.79
Mechanistic studies of a novel C-S lyase in ergothioneine biosynthesis: the involvement of a sulfenic acid intermediate. Sci Rep (2015) 0.78
Thermal inactivation of reduced ferredoxin (flavodoxin):NADP+ oxidoreductase from Escherichia coli. FEBS Lett (2002) 0.77
Crystallographic snapshots of sulfur insertion by lipoyl synthase. Proc Natl Acad Sci U S A (2016) 0.77
Combined Mössbauer spectroscopic, multi-edge X-ray absorption spectroscopic, and density functional theoretical study of the radical SAM enzyme spore photoproduct lyase. J Biol Inorg Chem (2014) 0.76
A combined computational and experimental investigation of the [2Fe-2S] cluster in biotin synthase. J Biol Inorg Chem (2009) 0.75
Semi-micro methods for analysis of labile sulfide and of labile sulfide plus sulfane sulfur in unusually stable iron-sulfur proteins. Anal Biochem (1983) 4.26
Rapid colorimetric micromethod for the quantitation of complexed iron in biological samples. Methods Enzymol (1988) 3.65
IscU as a scaffold for iron-sulfur cluster biosynthesis: sequential assembly of [2Fe-2S] and [4Fe-4S] clusters in IscU. Biochemistry (2000) 3.03
[2Fe-2S] to [4Fe-4S] cluster conversion in Escherichia coli biotin synthase. Biochemistry (1997) 1.65
Charges of nicotinamide adenine nucleotides and adenylate energy charge as regulatory parameters of the metabolism in Escherichia coli. J Biol Chem (1977) 1.57
Primary structures of Escherichia coli pyruvate formate-lyase and pyruvate-formate-lyase-activating enzyme deduced from the DNA nucleotide sequences. Eur J Biochem (1988) 1.55
Biotin synthase: purification, characterization as a [2Fe-2S]cluster protein, and in vitro activity of the Escherichia coli bioB gene product. Biochemistry (1994) 1.46
S-Adenosylmethionine-dependent reduction of lysine 2,3-aminomutase and observation of the catalytically functional iron-sulfur centers by electron paramagnetic resonance. Biochemistry (1998) 1.44
Biotin synthase contains two distinct iron-sulfur cluster binding sites: chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions. Biochemistry (2001) 1.43
Direct FeS cluster involvement in generation of a radical in lysine 2,3-aminomutase. Biochemistry (2000) 1.41
Pyruvate formate-lyase-activating enzyme: strictly anaerobic isolation yields active enzyme containing a [3Fe-4S](+) cluster. Biochem Biophys Res Commun (2000) 1.38
Biotin synthase, a new member of the family of enzymes which uses S-adenosylmethionine as a source of deoxyadenosyl radical. Biochem Biophys Res Commun (1997) 1.34
Lysine 2,3-aminomutase from Clostridium subterminale SB4: mass spectral characterization of cyanogen bromide-treated peptides and cloning, sequencing, and expression of the gene kamA in Escherichia coli. J Bacteriol (2000) 1.28
Biotin synthase from Escherichia coli, an investigation of the low molecular weight and protein components required for activity in vitro. J Biol Chem (1995) 1.25
Escherichia coli biotin synthase: an investigation into the factors required for its activity and its sulfur donor. Arch Biochem Biophys (1996) 1.24
Biotin synthase mechanism: on the origin of sulphur. FEBS Lett (1998) 1.23
Flavodoxin is required for conversion of dethiobiotin to biotin in Escherichia coli. Eur J Biochem (1994) 1.20
Iron-sulfur cluster interconversions in biotin synthase: dissociation and reassociation of iron during conversion of [2Fe-2S] to [4Fe-4S] clusters. Biochemistry (2000) 1.19
Iron-sulfur center of biotin synthase and lipoate synthase. Biochemistry (2000) 1.16
Lysine 2,3-aminomutase: is adenosylmethionine a poor man's adenosylcobalamin? FASEB J (1993) 1.16
Generation of the glycyl radical of the anaerobic Escherichia coli ribonucleotide reductase requires a specific activating enzyme. J Biol Chem (1995) 1.12
Conversion of dethiobiotin to biotin in cell-free extracts of Escherichia coli. Biosci Biotechnol Biochem (1992) 1.10
Transfer of sulfur to biotin from biotin synthase (BioB protein). Biochem Biophys Res Commun (1999) 1.10
Biotin biosynthesis in higher plant cells. Identification of intermediates. Eur J Biochem (1993) 1.06
Mössbauer studies of Escherichia coli biotin synthase: evidence for reversible interconversion between [2Fe-2S](2+) and [4Fe-4S](2+) clusters. FEBS Lett (1999) 1.02
Lysine 2,3-aminomutase and trans-4,5-dehydrolysine: characterization of an allylic analogue of a substrate-based radical in the catalytic mechanism. Biochemistry (2000) 1.02
Biotin synthase from Escherichia coli: isolation of an enzyme-generated intermediate and stoichiometry of S-adenosylmethionine use. Biochem J (1998) 1.01
Highly purified biotin synthase can transform dethiobiotin into biotin in the absence of any other protein, in the presence of photoreduced deazaflavin. Biochem Biophys Res Commun (1995) 0.99
MioC is an FMN-binding protein that is essential for Escherichia coli biotin synthase activity in vitro. J Biol Chem (2000) 0.96
The origin of sulfur in biotin. Biochem Biophys Res Commun (1983) 0.96
On the mechanism of biotin synthase of Bacillus sphaericus. C R Acad Sci III (1994) 0.95
Enzyme-mediated sulfide production for the reconstitution of [2Fe-2S] clusters into apo-biotin synthase of Escherichia coli. Sulfide transfer from cysteine to biotin. Eur J Biochem (2000) 0.94
Effects of mutations in aspartate 14 on the spectroscopic properties of the [Fe3S4]+,0 clusters in Pyrococcus furiosus ferredoxin. Biochemistry (1999) 0.84
Biotin synthase contains two distinct iron-sulfur cluster binding sites: chemical and spectroelectrochemical analysis of iron-sulfur cluster interconversions. Biochemistry (2001) 1.43
Iron-sulfur cluster interconversions in biotin synthase: dissociation and reassociation of iron during conversion of [2Fe-2S] to [4Fe-4S] clusters. Biochemistry (2000) 1.19
Interaction of Escherichia coli cobalamin-dependent methionine synthase and its physiological partner flavodoxin: binding of flavodoxin leads to axial ligand dissociation from the cobalamin cofactor. Biochemistry (1997) 1.18
A synthetic module for the metH gene permits facile mutagenesis of the cobalamin-binding region of Escherichia coli methionine synthase: initial characterization of seven mutant proteins. Biochemistry (1996) 0.84
[Not Available]. Sem Med (1969) 0.75
Stereospecificity of folate binding to DNA photolyase from Escherichia coli. Biochemistry (1995) 0.75
[Not Available]. Sem Med (1969) 0.75
The problems of employee absence. Va Med Mon (1918) (1965) 0.75