Cell-free translation reconstituted with purified components.

Molecular mechanisms of mTOR-mediated translational control. Nat Rev Mol Cell Biol (2009) 13.89

Protein production and purification. Nat Methods (2008) 3.97

A vesicle bioreactor as a step toward an artificial cell assembly. Proc Natl Acad Sci U S A (2004) 3.69

Paper-based synthetic gene networks. Cell (2014) 2.79

Formation of the first peptide bond: the structure of EF-P bound to the 70S ribosome. Science (2009) 2.33

Quality control by the ribosome following peptide bond formation. Nature (2008) 2.15

Construction of an in vitro bistable circuit from synthetic transcriptional switches. Mol Syst Biol (2006) 2.00

Principles of cell-free genetic circuit assembly. Proc Natl Acad Sci U S A (2003) 1.75

Bimodal protein solubility distribution revealed by an aggregation analysis of the entire ensemble of Escherichia coli proteins. Proc Natl Acad Sci U S A (2009) 1.73

An integrated cell-free metabolic platform for protein production and synthetic biology. Mol Syst Biol (2008) 1.72

Protein synthesis by ribosomes with tethered subunits. Nature (2015) 1.63

Synthetic in vitro transcriptional oscillators. Mol Syst Biol (2011) 1.53

Development of an artificial cell, from self-organization to computation and self-reproduction. Proc Natl Acad Sci U S A (2011) 1.43

A systematic survey of in vivo obligate chaperonin-dependent substrates. EMBO J (2010) 1.41

Programming peptidomimetic syntheses by translating genetic codes designed de novo. Proc Natl Acad Sci U S A (2003) 1.38

Microscale to manufacturing scale-up of cell-free cytokine production--a new approach for shortening protein production development timelines. Biotechnol Bioeng (2011) 1.36

Post-termination complex disassembly by ribosome recycling factor, a functional tRNA mimic. EMBO J (2002) 1.33

Predicting the pathway involved in post-translational modification of elongation factor P in a subset of bacterial species. Biol Direct (2010) 1.32

YaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl-tRNA on stalled ribosomes. Nucleic Acids Res (2010) 1.31

Distinct XPPX sequence motifs induce ribosome stalling, which is rescued by the translation elongation factor EF-P. Proc Natl Acad Sci U S A (2013) 1.30

Complex binding of the FabR repressor of bacterial unsaturated fatty acid biosynthesis to its cognate promoters. Mol Microbiol (2011) 1.29

The role of ribosome recycling factor in dissociation of 70S ribosomes into subunits. RNA (2005) 1.29

RNA, but not protein partners, is directly responsible for translational silencing by a bacterial Hfq-binding small RNA. Proc Natl Acad Sci U S A (2008) 1.28

Synthetic translational regulation by an L7Ae-kink-turn RNP switch. Nat Chem Biol (2009) 1.28

Decoding in the absence of a codon by tmRNA and SmpB in the ribosome. Science (2012) 1.24

Species-independent translational leaders facilitate cell-free expression. Nat Biotechnol (2009) 1.22

An expanded set of amino acid analogs for the ribosomal translation of unnatural peptides. PLoS One (2007) 1.21

Cytosolic chaperonin protects folding intermediates of Gbeta from aggregation by recognizing hydrophobic beta-strands. Proc Natl Acad Sci U S A (2006) 1.20

Diverse backbone-cyclized peptides via codon reprogramming. Nat Chem Biol (2009) 1.18

Recruitment of a species-specific translational arrest module to monitor different cellular processes. Proc Natl Acad Sci U S A (2011) 1.16

Evolutionary conservation of reactions in translation. Microbiol Mol Biol Rev (2002) 1.16

Knot formation in newly translated proteins is spontaneous and accelerated by chaperonins. Nat Chem Biol (2011) 1.15

Nano-enabled synthetic biology. Mol Syst Biol (2007) 1.15

A cross-kingdom internal ribosome entry site reveals a simplified mode of internal ribosome entry. Mol Cell Biol (2005) 1.13

Function of the ribosomal E-site: a mutagenesis study. Nucleic Acids Res (2005) 1.12

A bacterial virulence protein promotes pathogenicity by inhibiting the bacterium's own F1Fo ATP synthase. Cell (2013) 1.11

Ribosomal synthesis of N-methyl peptides. J Am Chem Soc (2008) 1.10

Cotranslational folding increases GFP folding yield. Biophys J (2010) 1.09

Role of antibiotic ligand in nascent peptide-dependent ribosome stalling. Proc Natl Acad Sci U S A (2011) 1.07

Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket. J Am Chem Soc (2012) 1.06

Enzymatic aminoacylation of tRNA with unnatural amino acids. Proc Natl Acad Sci U S A (2006) 1.05

Cell-free protein expression under macromolecular crowding conditions. PLoS One (2011) 1.05

A leaderless mRNA can bind to mammalian 80S ribosomes and direct polypeptide synthesis in the absence of translation initiation factors. Mol Cell Biol (2006) 1.05

Global analysis of chaperone effects using a reconstituted cell-free translation system. Proc Natl Acad Sci U S A (2012) 1.05

The ABC-F protein EttA gates ribosome entry into the translation elongation cycle. Nat Struct Mol Biol (2014) 1.03

A single-step method for purification of active His-tagged ribosomes from a genetically engineered Escherichia coli. Nucleic Acids Res (2008) 1.03

Effective cotranslational folding of firefly luciferase without chaperones of the Hsp70 family. Protein Sci (2005) 1.02

Tools for characterizing bacterial protein synthesis inhibitors. Antimicrob Agents Chemother (2013) 1.01

Rapidly characterizing the fast dynamics of RNA genetic circuitry with cell-free transcription-translation (TX-TL) systems. ACS Synth Biol (2014) 1.01

Remotely activated protein-producing nanoparticles. Nano Lett (2012) 1.01

Roles of Tom70 in import of presequence-containing mitochondrial proteins. J Biol Chem (2009) 1.01

Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Res (2013) 1.00

Bases in the anticodon loop of tRNA(Ala)(GGC) prevent misreading. Nat Struct Mol Biol (2009) 1.00

In vitro evolution of α-hemolysin using a liposome display. Proc Natl Acad Sci U S A (2013) 1.00

Ribosomal protein S1 influences trans-translation in vitro and in vivo. Nucleic Acids Res (2007) 1.00

Revolutionizing membrane protein overexpression in bacteria. Microb Biotechnol (2009) 1.00

Multiplexed in vivo His-tagging of enzyme pathways for in vitro single-pot multienzyme catalysis. ACS Synth Biol (2012) 1.00

Efficient production of isotopically labeled proteins by cell-free synthesis: a practical protocol. J Biomol NMR (2004) 0.99

Implementation of cell-free biological networks at steady state. Proc Natl Acad Sci U S A (2013) 0.99

Liposome display for in vitro selection and evolution of membrane proteins. Nat Protoc (2014) 0.99

Cotranslational Protein Folding inside the Ribosome Exit Tunnel. Cell Rep (2015) 0.99

Protocols for implementing an Escherichia coli based TX-TL cell-free expression system for synthetic biology. J Vis Exp (2013) 0.98

In vitro genetic reconstruction of bacterial transcription initiation by coupled synthesis and detection of RNA polymerase holoenzyme. Nucleic Acids Res (2010) 0.97

Identifying the targets of aminoacyl-tRNA synthetase inhibitors by primer extension inhibition. Nucleic Acids Res (2013) 0.97

Expression optimization and synthetic gene networks in cell-free systems. Nucleic Acids Res (2011) 0.96

Recent progress toward the templated synthesis and directed evolution of sequence-defined synthetic polymers. Chem Biol (2009) 0.96

Cell-free protein synthesis from a release factor 1 deficient Escherichia coli activates efficient and multiple site-specific nonstandard amino acid incorporation. ACS Synth Biol (2014) 0.96

A novel way of amino acid-specific assignment in (1)H-(15)N HSQC spectra with a wheat germ cell-free protein synthesis system. J Biomol NMR (2004) 0.95

Filamentous morphology in GroE-depleted Escherichia coli induced by impaired folding of FtsE. J Bacteriol (2007) 0.95

Single-molecule imaging of full protein synthesis by immobilized ribosomes. Nucleic Acids Res (2008) 0.95

Decoding system for the AUA codon by tRNAIle with the UAU anticodon in Mycoplasma mobile. Nucleic Acids Res (2013) 0.95

Cell-free expression and stable isotope labelling strategies for membrane proteins. J Biomol NMR (2009) 0.95

Nascentome analysis uncovers futile protein synthesis in Escherichia coli. PLoS One (2011) 0.95

Cell-free metabolic engineering: biomanufacturing beyond the cell. Biotechnol J (2014) 0.94

In vitro selection of highly modified cyclic peptides that act as tight binding inhibitors. J Am Chem Soc (2012) 0.94

Impact of methylations of m2G966/m5C967 in 16S rRNA on bacterial fitness and translation initiation. Nucleic Acids Res (2012) 0.94

Profligate biotin synthesis in α-proteobacteria - a developing or degenerating regulatory system? Mol Microbiol (2013) 0.94

Forced extraction of targeted components from complex macromolecular assemblies. Proc Natl Acad Sci U S A (2008) 0.94

Characterization of the emergent properties of a synthetic quasi-cellular system. BMC Bioinformatics (2012) 0.94

The origins of time-delay in template biopolymerization processes. PLoS Comput Biol (2010) 0.93

Rational design of an orthogonal tryptophanyl nonsense suppressor tRNA. Nucleic Acids Res (2010) 0.93

Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae. J Bacteriol (2011) 0.93

A highly purified, fluorescently labeled in vitro translation system for single-molecule studies of protein synthesis. Methods Enzymol (2010) 0.92

Inhibition of antiassociation activity of translation initiation factor 3 by paromomycin. Antimicrob Agents Chemother (2006) 0.92

Kinetics of stop codon recognition by release factor 1. Biochemistry (2009) 0.92

Cell-Sized confinement in microspheres accelerates the reaction of gene expression. Sci Rep (2012) 0.92

Non-standard amino acid incorporation into proteins using Escherichia coli cell-free protein synthesis. Front Chem (2014) 0.92

Directed evolution of multivalent glycopeptides tightly recognized by HIV antibody 2G12. J Am Chem Soc (2014) 0.91

In vitro experimental system for analysis of transcription-translation coupling. Nucleic Acids Res (2011) 0.91

Layer-by-layer cell membrane assembly. Nat Chem (2013) 0.91

A wheat germ cell-free system is a novel way to screen protein folding and function. Protein Sci (2003) 0.91

Synthetic gene brushes: a structure-function relationship. Mol Syst Biol (2008) 0.91

Comprehensive analysis of the effects of Escherichia coli ORFs on protein translation reaction. Mol Cell Proteomics (2008) 0.91

Versatility of trigger factor interactions with ribosome-nascent chain complexes. J Biol Chem (2010) 0.91

Structural basis for the substrate recognition and catalysis of peptidyl-tRNA hydrolase. Nucleic Acids Res (2012) 0.91

Compensatory evolution of a WW domain variant lacking the strictly conserved Trp residue. J Mol Evol (2007) 0.90

Ribosomal synthesis of dehydroalanine-containing peptides. J Am Chem Soc (2006) 0.90

The role of SmpB and the ribosomal decoding center in licensing tmRNA entry into stalled ribosomes. RNA (2011) 0.90

Real-time assay for testing components of protein synthesis. Nucleic Acids Res (2012) 0.90

A third lineage with two-piece tmRNA. Nucleic Acids Res (2004) 0.90

The International Registry of Acute Aortic Dissection (IRAD): new insights into an old disease. JAMA (2000) 12.26

Effect of a new immunosuppressive agent, FK 506, on heterotopic cardiac allotransplantation in the rat. Transplant Proc (1987) 12.04

Familial Parkinson disease gene product, parkin, is a ubiquitin-protein ligase. Nat Genet (2000) 7.83

Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor. Nature (1979) 6.56

A role for macrophage scavenger receptors in atherosclerosis and susceptibility to infection. Nature (1997) 5.55

Risk factors for hepatocellular carcinoma among patients with chronic liver disease. N Engl J Med (1993) 5.32

A two-step mechanism for the interaction of estradiol with rat uterus. Proc Natl Acad Sci U S A (1968) 4.77

Adenosine 3':5'-monophosphate-regulated phosphoprotein system of neuronal membranes. I. Solubilization, purification, and some properties of an endogenous phosphoprotein. J Biol Chem (1977) 4.33

Codon and amino-acid specificities of a transfer RNA are both converted by a single post-transcriptional modification. Nature (1988) 4.17

Incomplete flagellar structures in nonflagellate mutants of Salmonella typhimurium. J Bacteriol (1978) 4.07

Arginine-rich peptides. An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery. J Biol Chem (2000) 4.02

Cloning and sequence analysis of cDNA encoding the precursor of a human endothelium-derived vasoconstrictor peptide, endothelin: identity of human and porcine endothelin. FEBS Lett (1988) 3.96

A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli. Proc Natl Acad Sci U S A (1994) 3.74

Helicobacter pylori CagA protein can be tyrosine phosphorylated in gastric epithelial cells. J Exp Med (2000) 3.74

Nucleotide sequence of 5 S RNA from Torulopsis utilis. FEBS Lett (1974) 3.70

Microarray fabrication with covalent attachment of DNA using bubble jet technology. Nat Biotechnol (2000) 3.38

Cardiovascular anomaly, impaired actin bundling and resistance to Src-induced transformation in mice lacking p130Cas. Nat Genet (1998) 3.38

Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis. J Bacteriol (1984) 3.27

A synthetic inhibitor of histone deacetylase, MS-27-275, with marked in vivo antitumor activity against human tumors. Proc Natl Acad Sci U S A (1999) 3.26

Sequence of the fourth gene of human rotaviruses recovered from asymptomatic or symptomatic infections. J Virol (1988) 3.20

Sialic acid species as a determinant of the host range of influenza A viruses. J Virol (2000) 3.10

Unaltered secretion of beta-amyloid precursor protein in gelatinase A (matrix metalloproteinase 2)-deficient mice. J Biol Chem (1997) 3.09

Sarin poisoning in Tokyo subway. Lancet (1995) 3.06

Genetic mapping with SNP markers in Drosophila. Nat Genet (2001) 3.02

Primary structure, synthesis, and biological activity of rat endothelin, an endothelium-derived vasoconstrictor peptide. Proc Natl Acad Sci U S A (1988) 2.99

The der(17)t(X;17)(p11;q25) of human alveolar soft part sarcoma fuses the TFE3 transcription factor gene to ASPL, a novel gene at 17q25. Oncogene (2001) 2.97

Protein structure comparison using the markov transition model of evolution. Proteins (2000) 2.94

NEDD8 recruits E2-ubiquitin to SCF E3 ligase. EMBO J (2001) 2.94

Molecular cloning and characterization of a novel type of regulatory protein (GDI) for the rho proteins, ras p21-like small GTP-binding proteins. Oncogene (1990) 2.89

The Protein Mutant Database. Nucleic Acids Res (1999) 2.87

Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a. J Bacteriol (1993) 2.82

The Arabidopsis AHK4 histidine kinase is a cytokinin-binding receptor that transduces cytokinin signals across the membrane. Plant Cell Physiol (2001) 2.79

Phase-field model for binary alloys. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics (1999) 2.79

Incomplete flagellar structures in Escherichia coli mutants. J Bacteriol (1981) 2.77

Long-term clinical outcomes after unprotected left main trunk percutaneous revascularization in 279 patients. Circulation (2001) 2.77

Regulation of endogenous phosphorylation of specific proteins in synaptic membrane fractions from rat brain by adenosine 3':5'-monophosphate. J Biol Chem (1973) 2.66

Cortical mapping of gait in humans: a near-infrared spectroscopic topography study. Neuroimage (2001) 2.59

Uterine sutures at prior caesarean section and placenta accreta in subsequent pregnancy: a case-control study. BJOG (2014) 2.56

Transcriptive complex of Newcastle disease virus. I. Both L and P proteins are required to constitute an active complex. Virology (1983) 2.53

SLUG, a ces-1-related zinc finger transcription factor gene with antiapoptotic activity, is a downstream target of the E2A-HLF oncoprotein. Mol Cell (1999) 2.52

Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene. J Virol (1988) 2.44

Considerable time from the onset of plaque rupture and/or thrombi until the onset of acute myocardial infarction in humans: coronary angiographic findings within 1 week before the onset of infarction. Circulation (2000) 2.42

Isolation and characterization of fra-2, an additional member of the fos gene family. Proc Natl Acad Sci U S A (1990) 2.40

Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase. J Cell Biol (1979) 2.40

Rhamnolipids produced by Pseudomonas aeruginosa grown on n-paraffin (mixture of C 12 , C 13 and C 14 fractions). J Antibiot (Tokyo) (1971) 2.39

Specific inhibition of adherence of an oral strain of Bacteroides gingivalis 381 to epithelial cells by monoclonal antibodies against the bacterial fimbriae. Arch Oral Biol (1988) 2.39

Identification of cross-reactive and serotype 2-specific neutralization epitopes on VP3 of human rotavirus. J Virol (1988) 2.38

Differences in sialic acid-galactose linkages in the chicken egg amnion and allantois influence human influenza virus receptor specificity and variant selection. J Virol (1997) 2.38

Localization of a novel v-erbB-related gene, c-erbB-2, on human chromosome 17 and its amplification in a gastric cancer cell line. Mol Cell Biol (1986) 2.33

A cytotoxic ribonuclease targeting specific transfer RNA anticodons. Science (1999) 2.29

Metabolism of pentachlorophenol by a soil microbe. J Environ Sci Health B (1977) 2.28

Covalent modifier NEDD8 is essential for SCF ubiquitin-ligase in fission yeast. EMBO J (2000) 2.27

Network theory in autoimmunity. In vitro suppression of serum anti-DNA antibody binding to DNA by anti-idiotypic antibody in systemic lupus erythematosus. J Clin Invest (1981) 2.24

Surgical significance of anatomic variations of the hepatic artery. Am J Surg (1971) 2.23

Chloroplast transit peptides. The perfect random coil? FEBS Lett (1991) 2.20

Exclusive expression of C. elegans osm-3 kinesin gene in chemosensory neurons open to the external environment. J Mol Biol (1995) 2.20

Role of the extra G-C pair at the end of the acceptor stem of tRNA(His) in aminoacylation. Nucleic Acids Res (1989) 2.18

Cultivated corneal epithelial stem cell transplantation in ocular surface disorders. Ophthalmology (2001) 2.16

New graft-implanting method for thoracic aortic aneurysm or dissection with a stented graft. Circulation (1996) 2.14

A new cdc gene required for S phase entry of Schizosaccharomyces pombe encodes a protein similar to the cdc 10+ and SWI4 gene products. EMBO J (1992) 2.14

Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol (1988) 2.12

Discrimination of intracellular and extracellular proteins using amino acid composition and residue-pair frequencies. J Mol Biol (1994) 2.11

Trifluoroethanol-induced stabilization of the alpha-helical structure of beta-lactoglobulin: implication for non-hierarchical protein folding. J Mol Biol (1995) 2.09

Primary structure of chicken skeletal muscle and fibroblast alpha-actinins deduced from cDNA sequences. Eur J Biochem (1988) 2.08

White ball appearance in endoscopic ligation of bleeding esophageal varices. Gastrointest Endosc (1998) 2.08

The folding type of a protein is relevant to the amino acid composition. J Biochem (1986) 2.07

Mouse Ror2 receptor tyrosine kinase is required for the heart development and limb formation. Genes Cells (2000) 2.05

Antiproliferative activity of ecteinascidin 743 is dependent upon transcription-coupled nucleotide-excision repair. Nat Med (2001) 2.04

Streptococcal cytoplasmic pH is regulated by changes in amount and activity of a proton-translocating ATPase. J Biol Chem (1986) 2.03

Effects of combination treatment with FK506 and cyclosporine on survival time and vascular changes in renal-allograft-recipient dogs. Transplantation (1989) 2.03

LST-2, a human liver-specific organic anion transporter, determines methotrexate sensitivity in gastrointestinal cancers. Gastroenterology (2001) 2.02

Sequence of an intestinal cDNA encoding human gastric inhibitory polypeptide precursor. Proc Natl Acad Sci U S A (1987) 2.02

Walking speed as a good predictor for the onset of functional dependence in a Japanese rural community population. Age Ageing (2000) 1.99

Genetic heterogeneity of the human glutathione transferases: a complex of gene families. Pharmacol Ther (1990) 1.99

Inversion of the membrane topology of SecG coupled with SecA-dependent preprotein translocation. Cell (1996) 1.98

Glue embolization for gastroduodenal ulcer bleeding: contribution to hemodynamics and healing process. Acta Radiol (2013) 1.98

Widespread distribution of protein I in the central and peripheral nervous systems. Proc Natl Acad Sci U S A (1979) 1.98

Predictors for functional decline among nondisabled older Japanese living in a community during a 3-year follow-up. J Am Geriatr Soc (2000) 1.98

In vivo klotho gene delivery protects against endothelial dysfunction in multiple risk factor syndrome. Biochem Biophys Res Commun (2000) 1.97

Expression of glucose transporter-1 in human gastric carcinoma: association with tumor aggressiveness, metastasis, and patient survival. Cancer (2001) 1.96

Immunocytochemical localization, in synapses, of protein I, an endogenous substrate for protein kinases in mammalian brain. Proc Natl Acad Sci U S A (1979) 1.96

Non-universal decoding of the leucine codon CUG in several Candida species. Nucleic Acids Res (1993) 1.94

Expression of processed core protein of hepatitis C virus in mammalian cells. J Virol (1991) 1.91

High sensitivity C-reactive protein in asthma. Eur Respir J (2006) 1.91

Prevalence of radiographic knee osteoarthritis and its association with knee pain in the elderly of Japanese population-based cohorts: the ROAD study. Osteoarthritis Cartilage (2009) 1.90

Rheologic properties of senescent erythrocytes: loss of surface area and volume with red blood cell age. Blood (1992) 1.89

Amplification of the Streptococcus faecalis proton-translocating ATPase by a decrease in cytoplasmic pH. J Bacteriol (1984) 1.87

Microbial degradation of natural rubber vulcanizates. Appl Environ Microbiol (1985) 1.85

A novel lysine-substituted nucleoside in the first position of the anticodon of minor isoleucine tRNA from Escherichia coli. J Biol Chem (1988) 1.85

Microbial flora in the deepest sea mud of the Mariana Trench. FEMS Microbiol Lett (1997) 1.84

A dominant negative mutant of sar1 GTPase inhibits protein transport from the endoplasmic reticulum to the Golgi apparatus in tobacco and Arabidopsis cultured cells. Plant J (2000) 1.84

Phosphorylation-dependent regulation of the interaction of amyloid precursor protein with Fe65 affects the production of beta-amyloid. J Biol Chem (2001) 1.83

Characterization of a trypsin-like protease from the bacterium Bacteroides gingivalis isolated from human dental plaque. Arch Oral Biol (1984) 1.83

Intramitochondrial filamentous bodies in the thick limb of henle of the rat kidney. J Cell Biol (1967) 1.83

Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: evolutionary implications. Proc Natl Acad Sci U S A (1992) 1.82

Mutations of the epigenetics-modifying gene (DNMT3a, TET2, IDH1/2) at diagnosis may induce FLT3-ITD at relapse in de novo acute myeloid leukemia. Leukemia (2012) 1.82