Published in Gene on August 08, 2001
Design parameters to control synthetic gene expression in Escherichia coli. PLoS One (2009) 3.38
Gene Designer: a synthetic biology tool for constructing artificial DNA segments. BMC Bioinformatics (2006) 2.85
Predicting Shine-Dalgarno sequence locations exposes genome annotation errors. PLoS Comput Biol (2006) 1.55
A codon window in mRNA downstream of the initiation codon where NGG codons give strongly reduced gene expression in Escherichia coli. Nucleic Acids Res (2004) 1.47
You're one in a googol: optimizing genes for protein expression. J R Soc Interface (2009) 1.39
Analysis of type II secretion of recombinant pneumococcal PspA and PspC in a Salmonella enterica serovar Typhimurium vaccine with regulated delayed antigen synthesis. Infect Immun (2008) 1.23
Broad-host-range plasmid pJB658 can be used for industrial-level production of a secreted host-toxic single-chain antibody fragment in Escherichia coli. Appl Environ Microbiol (2004) 1.21
The presence of N-terminal secretion signal sequences leads to strong stimulation of the total expression levels of three tested medically important proteins during high-cell-density cultivations of Escherichia coli. Appl Environ Microbiol (2006) 1.13
The Escherichia coli lipB gene encodes lipoyl (octanoyl)-acyl carrier protein:protein transferase. J Bacteriol (2003) 1.11
Architecture of the Bacteroides cellulosolvens cellulosome: description of a cell surface-anchoring scaffoldin and a family 48 cellulase. J Bacteriol (2004) 1.07
Initiation of mRNA translation in bacteria: structural and dynamic aspects. Cell Mol Life Sci (2015) 0.92
Cis control of gene expression in E.coli by ribosome queuing at an inefficient translational stop signal. EMBO J (2002) 0.90
A top-down/bottom-up study of the ribosomal proteins of Caulobacter crescentus. J Proteome Res (2007) 0.89
A novel methodology for enhanced and consistent heterologous expression of unmodified human cytochrome P450 1B1 (CYP1B1). PLoS One (2014) 0.87
BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism. Nucleic Acids Res (2010) 0.86
Molecular phylogenetics and evolutionary history of ariid catfishes revisited: a comprehensive sampling. BMC Evol Biol (2009) 0.86
Efficient expression of gene variants that harbour AGA codons next to the initiation codon. Nucleic Acids Res (2007) 0.84
Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli. Nucleic Acids Res (2007) 0.79
An mRNA sequence derived from a programmed frameshifting signal decreases codon discrimination during translation initiation. RNA (2006) 0.79
Use of signal sequences as an in situ removable sequence element to stimulate protein synthesis in cell-free extracts. Nucleic Acids Res (2006) 0.78
Transient erythromycin resistance phenotype associated with peptidyl-tRNA drop-off on early UGG and GGG codons. J Bacteriol (2007) 0.78
Follow the leader: preference for specific amino acids directly following the initial methionine in proteins of different organisms. Genomics Proteomics Bioinformatics (2010) 0.78
Minigene-like inhibition of protein synthesis mediated by hungry codons near the start codon. Nucleic Acids Res (2008) 0.75
Huh-7 cell line as an alternative cultural model for the production of human like erythropoietin (EPO). J Transl Med (2011) 0.75
Influence of the second amino acid on recombinant protein expression. Protein Expr Purif (2010) 0.75
Selection on start codons in prokaryotes and potential compensatory nucleotide substitutions. Sci Rep (2017) 0.75
Ribosomal selection of mRNAs with degenerate initiation triplets. Nucleic Acids Res (2017) 0.75
Phase I safety and pharmacokinetic study of recombinant human anti-vascular endothelial growth factor in patients with advanced cancer. J Clin Oncol (2001) 4.97
Minimally important differences were estimated for the Functional Assessment of Cancer Therapy-Colorectal (FACT-C) instrument using a combination of distribution- and anchor-based approaches. J Clin Epidemiol (2005) 4.29
A synthetic IgG-binding domain based on staphylococcal protein A. Protein Eng (1989) 3.31
Isolation of mutants of Escherichia coli lac king 5-methyluracil in transfer ribonucleic acid or 1-methylguanine in ribosomal RNA. J Mol Biol (1970) 2.61
A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA nonsense suppressors. Mol Gen Genet (1984) 2.03
Emerging understanding of translation termination. Cell (1996) 2.02
The second to last amino acid in the nascent peptide as a codon context determinant. EMBO J (1994) 1.98
Translation rates and misreading characteristics of rpsD mutants in Escherichia coli. Mol Gen Genet (1982) 1.95
Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis. EMBO J (1998) 1.67
Codon bias at the 3'-side of the initiation codon is correlated with translation initiation efficiency in Escherichia coli. Gene (2001) 1.65
Influence of modification next to the anticodon in tRNA on codon context sensitivity of translational suppression and accuracy. J Bacteriol (1986) 1.65
A procedure for isolation of spontaneous mutants with temperature sensitive of RNA and/or protein. Mol Gen Genet (1977) 1.63
Structure of the C-terminal end of the nascent peptide influences translation termination. EMBO J (1996) 1.60
Phase Ib trial of intravenous recombinant humanized monoclonal antibody to vascular endothelial growth factor in combination with chemotherapy in patients with advanced cancer: pharmacologic and long-term safety data. J Clin Oncol (2001) 1.58
Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase. Proc Natl Acad Sci U S A (1978) 1.53
Analysis of rpsD mutations in Escherichia coli. III. Effects of rpsD mutations on expression of some ribosomal protein genes. Mol Gen Genet (1979) 1.52
Codon choice and potential complementarity between mRNA downstream of the initiation codon and bases 1471-1480 in 16S ribosomal RNA affects expression of glnS. Nucleic Acids Res (1991) 1.51
Rhizobium meliloti anthranilate synthase gene: cloning, sequence, and expression in Escherichia coli. J Bacteriol (1989) 1.47
The influence of 5' codon context on translation termination in Saccharomyces cerevisiae. Eur J Biochem (1998) 1.46
A gene fusion system for generating antibodies against short peptides. Gene (1987) 1.42
Involvement of ribosomal protein L7/L12 in control of translational accuracy. Proc Natl Acad Sci U S A (1985) 1.35
Characterization of a succinate dehydrogenase complex solubilized from the cytoplasmic membrane of Bacillus subtilis with the nonionic detergent Triton X-100. J Bacteriol (1979) 1.29
Characterization of succinic dehydrogenase mutants of Bacillus subtilis by crossed immunoelectrophoresis. J Bacteriol (1978) 1.27
The Reaction Level Scale (RLS85). Manual and guidelines. Acta Neurochir (Wien) (1988) 1.24
Efficient secretion and purification of human insulin-like growth factor I with a gene fusion vector in Staphylococci. Nucleic Acids Res (1985) 1.18
UGA codon context which spans three codons. Reversal by ms2i6A37 in tRNA, mutation in rpsD(S4) or streptomycin. J Mol Biol (1993) 1.16
Accumulation of a mRNA decay intermediate by ribosomal pausing at a stop codon. Nucleic Acids Res (1996) 1.15
Role of heme in synthesis and membrane binding of succinic dehydrogenase in Bacillus subtilis. J Bacteriol (1979) 1.14
Analysis of rpsD mutations in Escherichia coli. I. Comparison of mutants with various alterations in ribosomal protein S4. Mol Gen Genet (1979) 1.13
Growth phase dependent stop codon readthrough and shift of translation reading frame in Escherichia coli. FEBS Lett (1998) 1.07
Studies on microbial RNA. V. A comparison of the in vivo methylated components of ribosomal RNA from Escherichia coli and Saccharomyces cerevisiae. Biochim Biophys Acta (1968) 1.06
A comparison of the Glasgow Coma Scale and the Reaction Level Scale (RLS85). J Neurosurg (1988) 1.05
Co-existing structures of an mRNA stability determinant. The 5' region of the Escherichia coli and Serratia marcescens ompA mRNA. J Mol Biol (1993) 1.02
Ribosome activity and modification of 16S RNA are influenced by deletion of ribosomal protein S20. Mol Microbiol (1993) 1.02
Escherichia coli rpoC397 encodes a temperature-sensitive C-terminal frameshift in the beta' subunit of RNA polymerase that blocks growth of bacteriophage P2. J Bacteriol (1996) 1.02
Genetic implication for an interaction between release factor one and ribosomal protein L7/L12 in vivo. J Mol Biol (1994) 0.99
Expression of human insulin-like growth factor I in bacteria: use of optimized gene fusion vectors to facilitate protein purification. Biochemistry (1987) 0.98
Evaluation of the pain suppressive effect of different frequencies of peripheral electrical stimulation in chronic pain conditions. Acta Orthop Scand (1976) 0.97
Production of specific antibodies against protein A fusion proteins. EMBO J (1986) 0.97
Functional interaction between release factor one and P-site peptidyl-tRNA on the ribosome. J Mol Biol (1996) 0.97
Only the last amino acids in the nascent peptide influence translation termination in Escherichia coli genes. FEBS Lett (1997) 0.96
On acupuncture analgesia and the mechanism of pain. Am J Chin Med (Gard City N Y) (1975) 0.96
Isolation of cDNA sequences coding for a part of human tissue plasminogen activator. Proc Natl Acad Sci U S A (1983) 0.93
Functional interactions between translation, transcription and ppGpp in growing Escherichia coli. Biochim Biophys Acta (1994) 0.93
Dual affinity fusion approach and its use to express recombinant human insulin-like growth factor II. Proc Natl Acad Sci U S A (1989) 0.91
Temperature-sensitive mutants in cysteinyl-tRNA ligase of E. coli K-12. Mol Gen Genet (1979) 0.91
A recombinant Escherichia coli heat-stable enterotoxin (STa) fusion protein eliciting anti-STa neutralizing antibodies. FEMS Microbiol Lett (1991) 0.90
A temperature-sensitive mutant in prolinyl-tRNA ligase of Escherichia coli K-12. Mol Gen Genet (1980) 0.88
Bacillus subtilis mutant with temperature-sensitive net synthesis of phosphatidylethanolamine. J Bacteriol (1977) 0.88
The analysis of translational activity using a reporter gene constructed from repeats of an antibody-binding domain from protein A. Methods Mol Biol (1998) 0.88
Synthesis and secretion of a fibrinolytically active tissue-type plasminogen activator variant in Escherichia coli. Gene (1991) 0.88
Antagonistic effects of mutant elongation factor Tu and ribosomal protein S12 on control of translational accuracy, suppression and cellular growth. Biochimie (1988) 0.88
Analysis of rpsD mutations in Escherichia coli. IV. Accumulation of minor forms of protein S7(K) in ribosomes of rpsD mutant strains due to translational read-through. Mol Gen Genet (1980) 0.88
A large convexity meningioma followed by liquorrhoea from a nasal encephalocele. A case report. Acta Neurochir (Wien) (1981) 0.87
Partial purification of ribosomal RNA(m1G)- and rRNA(m2G)-methylases from Escherichia coli and demonstration of some proteins affecting their apparent activity. Biochim Biophys Acta (1973) 0.87
Primary structures of mutationally altered ribosomal protein L7/L12 and their effects on cellular growth and translational accuracy. Eur J Biochem (1986) 0.86
Current reporting of responsiveness in acute cerebral disorders. A survey of the neurosurgical literature. J Neurosurg (1988) 0.86
Starvation in vivo for aminoacyl-tRNA increases the spatial separation between the two ribosomal subunits. Cell (1994) 0.86
Regulation of tryptophan genes in Rhizobium leguminosarum. J Bacteriol (1982) 0.86
Human immunodeficiency viral protease is catalytically active as a fusion protein: characterization of the fusion and native enzymes produced in Escherichia coli. Arch Biochem Biophys (1990) 0.86
Functional interactions in vivo between suppressor tRNA and mutationally altered ribosomal protein S4. Mol Gen Genet (1986) 0.85
Functional interaction between tRNA2Gly2 at the ribosomal P-site and RF1 during termination at UAG. J Mol Biol (1998) 0.83
Base 2661 in Escherichia coli 23S rRNA influences the binding of elongation factor Tu during protein synthesis in vivo. Eur J Biochem (1991) 0.83
The temperature sensitive mutant 72c. II. Accumulation at high temperature of ppGpp and pppGpp in the presence of protein synthesis. Mol Gen Genet (1978) 0.83
Structure of a combination of Functional Independence Measure and Instrumental Activity Measure items in community-living persons: a study of individuals with cerebral palsy and spina bifida. Arch Phys Med Rehabil (1996) 0.82
Antisuppression by a mutation in rpsM(S13) giving a shortened ribosomal protein S13. Biochim Biophys Acta (1994) 0.82
Is efficiency of suppressor tRNAs controlled at the level of ribosomal proofreading in vivo? J Bacteriol (1988) 0.82
Visualization of a large conformation change of ribosomes in Escherichia coli cells starved for tryptophan or treated with kirromycin. Exp Cell Res (1998) 0.82
Electro-acupuncture. Effect on pain threshold measured with electrical stimulation of teeth. Brain Res (1973) 0.81
Formation in vitro of 1-methylguanine in 23-s RNA from Escherichia coli. The effects of spermidine and two proteins. Biochim Biophys Acta (1973) 0.81
Post-operative thromboembolism in neurosurgery. A study on the prophylactic effect of calf muscle stimulation plus dextran compared to low-dose heparin. Acta Neurochir (Wien) (1986) 0.81
Automated correlation and averaging of three-dimensional reconstructions obtained by electron tomography. J Struct Biol (1997) 0.81
Enhanced immunogenicity of recombinant peptide fusions containing multiple copies of a heterologous T helper epitope. Eur J Immunol (1990) 0.80
Fusion protein approach to improve the crystal quality of cytochrome bo(3) ubiquinol oxidase from Escherichia coli. Biochim Biophys Acta (2000) 0.80
Antisuppression by mutations in elongation factor Tu. Eur J Biochem (1990) 0.79
Characterization of an extended form of recombinant human insulin-like growth factor II. J Biol Chem (1991) 0.79
Thrombin and H64A subtilisin cleavage of fusion proteins for preparation of human recombinant parathyroid hormone. J Protein Chem (1991) 0.79
Mutations in the tryptophanyl-transfer ribonucleic acid ligase of E. coli causing temperature-sensitivity for growth. Mol Gen Genet (1978) 0.79
The temperature sensitive mutant 72c. I. Pleiotropic growth behaviour and changed response to some antibiotics and mutations in the transcription or translation apparatus. Mol Gen Genet (1978) 0.79
Fibrinopeptide A and fibrinogen fragment B beta 15-42 and their relation to the operative trauma and post-operative thromboembolism in neurosurgical patients. Acta Neurochir (Wien) (1987) 0.78
An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I. J Protein Chem (1992) 0.78
Interaction between a mutant release factor one and P-site peptidyl-tRNA is influenced by the identity of the two bases downstream of the stop codon UAG. FEBS Lett (1999) 0.78
Influence of the last amino acid in the nascent peptide on EF-Tu during decoding. Biochimie (1996) 0.77
Fusions to the 5' end of a gene encoding a two-domain analogue of staphylococcal protein A. J Biotechnol (1992) 0.77
On-line strategies for determining trace levels of nitroaromatic explosives and related compounds in water. J Chromatogr A (2006) 0.76
Expression and characterization of a recombinant human parathyroid hormone secreted by Escherichia coli employing the staphylococcal protein A promoter and signal sequence. J Biol Chem (1990) 0.76
Assessment of responsiveness in acute cerebral disorders. A multicentre study on the reaction level scale (RLS 85). Acta Neurochir (Wien) (1988) 0.76
Three human interferon-alpha 2 subvariants disclose structural and functional differences. Eur J Biochem (1990) 0.75
The efficiency of a cis-cleaving ribozyme in an mRNA coding region is influenced by the translating ribosome in vivo. Nucleic Acids Res (1997) 0.75
Effects of growth conditions and mutations in RNA polymerase on translational activity in vitro in Escherichia coli. Mol Gen Genet (1980) 0.75
Purification and characterization of recombinant human insulin-like growth factor II (IGF-II) expressed as a secreted fusion protein in Escherichia coli. Biotechnol Appl Biochem (1991) 0.75
Increase of pain threshold as a function of conditionsing electrical stimulation. An experimental study with application to electro-acupuncture for pain suppression. Am J Chin Med (Gard City N Y) (1975) 0.75
[Low frequent transcutaneous electrical stimulation for pain relief during labor]. Lakartidningen (1976) 0.75
Changes in synaptic function induced by blockage of axonal transport in the rabbit optic pathway. Brain Res (1978) 0.75
Differential stability of recombinant human insulin-like growth factor II in Escherichia coli and Staphylococcus aureus. J Biotechnol (1990) 0.75
The relationship of hallucinations to the depth structures of the temporal lobe. Acta Neurochir (Wien) (1977) 0.75
[Prevention of thrombosis using heparin in neurosurgical intervention]. Lakartidningen (1980) 0.75
Acute head injuries: cooperative efforts in clinical assessment. J Neurosurg (1981) 0.75
Letter: Electro-acupuncture and pain threshold. Lancet (1973) 0.75