Published in Structure on September 01, 2002
Enhancing antibody Fc heterodimer formation through electrostatic steering effects: applications to bispecific molecules and monovalent IgG. J Biol Chem (2010) 1.60
A synthetic coiled-coil interactome provides heterospecific modules for molecular engineering. J Am Chem Soc (2010) 1.32
Semirational design of Jun-Fos coiled coils with increased affinity: Universal implications for leucine zipper prediction and design. Proc Natl Acad Sci U S A (2006) 1.27
Coiled-coil response to mechanical force: global stability and local cracking. Biophys J (2013) 0.98
Unique stabilizing interactions identified in the two-stranded alpha-helical coiled-coil: crystal structure of a cortexillin I/GCN4 hybrid coiled-coil peptide. Protein Sci (2003) 0.90
Molecular dynamics guided study of salt bridge length dependence in both fluorinated and non-fluorinated parallel dimeric coiled-coils. Proteins (2009) 0.86
Increasing the affinity of selective bZIP-binding peptides through surface residue redesign. Protein Sci (2014) 0.86
Epstein-Barr virus IL-10 engages IL-10R1 by a two-step mechanism leading to altered signaling properties. J Biol Chem (2012) 0.83
Kinetic analysis of cytokine-mediated receptor assembly using engineered FC heterodimers. Protein Sci (2013) 0.77
Oligomerization-primed coiled-coil domain interaction with Ubc13 confers processivity to TRAF6 ubiquitin ligase activity. Nat Commun (2017) 0.75
Automated protein crystal structure determination using ELVES. Proc Natl Acad Sci U S A (2004) 4.10
Global landscape of HIV-human protein complexes. Nature (2011) 3.70
Engineering novel binding proteins from nonimmunoglobulin domains. Nat Biotechnol (2005) 3.67
High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol (2004) 3.34
Hidden alternative structures of proline isomerase essential for catalysis. Nature (2009) 3.28
Autofluorescent proteins with excitation in the optical window for intravital imaging in mammals. Chem Biol (2009) 3.11
Accessing protein conformational ensembles using room-temperature X-ray crystallography. Proc Natl Acad Sci U S A (2011) 2.92
HIV-1 Tat and host AFF4 recruit two transcription elongation factors into a bifunctional complex for coordinated activation of HIV-1 transcription. Mol Cell (2010) 2.91
Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J Mol Biol (2003) 2.81
Saccharomyces cerevisiae septins: supramolecular organization of heterooligomers and the mechanism of filament assembly. Proc Natl Acad Sci U S A (2008) 2.46
Structure of Mycobacterium tuberculosis PknB supports a universal activation mechanism for Ser/Thr protein kinases. Nat Struct Biol (2003) 2.27
Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display. Nat Biotechnol (2006) 2.18
Automated electron-density sampling reveals widespread conformational polymorphism in proteins. Protein Sci (2010) 2.16
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
An alternate conformation and a third metal in PstP/Ppp, the M. tuberculosis PP2C-Family Ser/Thr protein phosphatase. Structure (2004) 1.99
Designed to be stable: crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci U S A (2003) 1.98
Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure (2007) 1.93
Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability. J Mol Biol (2005) 1.90
A label-free immunosensor array using single-chain antibody fragments. Proc Natl Acad Sci U S A (2005) 1.85
Biophysical properties of human antibody variable domains. J Mol Biol (2003) 1.80
Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. Structure (2005) 1.75
A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J Mol Biol (2007) 1.71
Suite of three protein crystallography beamlines with single superconducting bend magnet as the source. J Synchrotron Radiat (2004) 1.70
In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods (2004) 1.69
Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat Methods (2007) 1.68
Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins. J Mol Biol (2007) 1.67
Design of multivalent complexes using the barnase*barstar module. Nat Biotechnol (2003) 1.65
Direct identification of ligand-receptor interactions on living cells and tissues. Nat Biotechnol (2012) 1.64
Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design. Curr Opin Biotechnol (2005) 1.64
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. Proc Natl Acad Sci U S A (2008) 1.63
Protein-protein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae. Mol Biol Cell (2004) 1.63
The energy landscape of adenylate kinase during catalysis. Nat Struct Mol Biol (2015) 1.62
Evolution of three human GPCRs for higher expression and stability. J Mol Biol (2011) 1.62
DARPins and other repeat protein scaffolds: advances in engineering and applications. Curr Opin Biotechnol (2011) 1.61
Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J Mol Biol (2008) 1.57
Human Polymerase-Associated Factor complex (PAFc) connects the Super Elongation Complex (SEC) to RNA polymerase II on chromatin. Proc Natl Acad Sci U S A (2011) 1.56
Depletion of antibiotic targets has widely varying effects on growth. Proc Natl Acad Sci U S A (2011) 1.54
Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. Cancer Res (2010) 1.51
Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity. J Biol Chem (2004) 1.46
Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. Proc Natl Acad Sci U S A (2013) 1.46
A phosphorylated pseudokinase complex controls cell wall synthesis in mycobacteria. Sci Signal (2012) 1.41
Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family. J Mol Biol (2003) 1.39
Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering. Methods (2004) 1.37
Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core. J Mol Biol (2007) 1.33
Structure of a kinesin-tubulin complex and implications for kinesin motility. Nat Struct Mol Biol (2013) 1.31
Structural perturbation and compensation by directed evolution at physiological temperature leads to thermostabilization of beta-lactamase. Biochemistry (2005) 1.29
M. tuberculosis Ser/Thr protein kinase D phosphorylates an anti-anti-sigma factor homolog. PLoS Pathog (2007) 1.28
The TB structural genomics consortium: providing a structural foundation for drug discovery. Curr Drug Targets Infect Disord (2002) 1.28
Fragment-based substrate activity screening method for the identification of potent inhibitors of the Mycobacterium tuberculosis phosphatase PtpB. J Am Chem Soc (2007) 1.28
Semirational design of Jun-Fos coiled coils with increased affinity: Universal implications for leucine zipper prediction and design. Proc Natl Acad Sci U S A (2006) 1.27
Protein motions promote catalysis. Chem Biol (2004) 1.27
Purification and characterization of HIV-human protein complexes. Methods (2010) 1.26
PEGylation and multimerization of the anti-p185HER-2 single chain Fv fragment 4D5: effects on tumor targeting. J Biol Chem (2006) 1.26
Mycobacterium tuberculosis serine/threonine kinases PknB, PknD, PknE, and PknF phosphorylate multiple FHA domains. Protein Sci (2005) 1.26
Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework. J Biol Chem (2002) 1.25
Engineered proteins as specific binding reagents. Curr Opin Biotechnol (2005) 1.24
Moving metal ions through ferritin-protein nanocages from three-fold pores to catalytic sites. J Am Chem Soc (2010) 1.23
Biophysical properties of camelid V(HH) domains compared to those of human V(H)3 domains. Biochemistry (2002) 1.22
Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy. Biophys J (2005) 1.20
Folding of a designed simple ankyrin repeat protein. Protein Sci (2004) 1.20
Auto-activation mechanism of the Mycobacterium tuberculosis PknB receptor Ser/Thr kinase. EMBO J (2008) 1.19
Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode. J Biol Chem (2009) 1.19
Structure/function studies of Ser/Thr and Tyr protein phosphorylation in Mycobacterium tuberculosis. J Mol Microbiol Biotechnol (2005) 1.19
Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem (2005) 1.17
Exploring subdomain cooperativity in T4 lysozyme I: structural and energetic studies of a circular permutant and protein fragment. Protein Sci (2007) 1.17
A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc Natl Acad Sci U S A (2012) 1.16
Sensor domain of the Mycobacterium tuberculosis receptor Ser/Thr protein kinase, PknD, forms a highly symmetric beta propeller. J Mol Biol (2004) 1.15
Selection and characterization of Her2 binding-designed ankyrin repeat proteins. J Biol Chem (2006) 1.15
A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett (2003) 1.15
Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli. Proc Natl Acad Sci U S A (2014) 1.15
Protein PEGylation decreases observed target association rates via a dual blocking mechanism. Mol Pharmacol (2005) 1.14
A recombinant immunotoxin derived from a humanized epithelial cell adhesion molecule-specific single-chain antibody fragment has potent and selective antitumor activity. Clin Cancer Res (2003) 1.13
Allosteric activation by dimerization of the PknD receptor Ser/Thr protein kinase from Mycobacterium tuberculosis. J Biol Chem (2007) 1.11
Structural basis for selective inhibition of Mycobacterium tuberculosis protein tyrosine phosphatase PtpB. Structure (2007) 1.10
Site-saturation mutagenesis of Tyr-105 reveals its importance in substrate stabilization and discrimination in TEM-1 beta-lactamase. J Biol Chem (2004) 1.10
Mycobacterium tuberculosis protein tyrosine phosphatase PtpB structure reveals a diverged fold and a buried active site. Structure (2005) 1.10
Protein structural ensembles are revealed by redefining X-ray electron density noise. Proc Natl Acad Sci U S A (2013) 1.10
Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations. Proc Natl Acad Sci U S A (2012) 1.09
Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy. Biophys J (2007) 1.09
A conserved dimer and global conformational changes in the structure of apo-PknE Ser/Thr protein kinase from Mycobacterium tuberculosis. J Mol Biol (2006) 1.08
DARPins: an efficient targeting domain for lentiviral vectors. Mol Ther (2011) 1.08
HIV-1 Tat recruits transcription elongation factors dispersed along a flexible AFF4 scaffold. Proc Natl Acad Sci U S A (2012) 1.08
The AFF4 scaffold binds human P-TEFb adjacent to HIV Tat. Elife (2013) 1.08
Hyaloperonospora arabidopsidis ATR1 effector is a repeat protein with distributed recognition surfaces. Proc Natl Acad Sci U S A (2011) 1.08
Ferritin protein nanocage ion channels: gating by N-terminal extensions. J Biol Chem (2012) 1.08
Extremely elevated room-temperature kinetic isotope effects quantify the critical role of barrier width in enzymatic C-H activation. J Am Chem Soc (2014) 1.07
Turnover-based in vitro selection and evolution of biocatalysts from a fully synthetic antibody library. Nat Biotechnol (2003) 1.06
Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach. Biochemistry (2003) 1.06
An atypical receiver domain controls the dynamic polar localization of the Myxococcus xanthus social motility protein FrzS. Mol Microbiol (2007) 1.06
Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation. J Mol Biol (2006) 1.06
Designed ankyrin repeat proteins (DARPins) from research to therapy. Methods Enzymol (2012) 1.06
AFF1 is a ubiquitous P-TEFb partner to enable Tat extraction of P-TEFb from 7SK snRNP and formation of SECs for HIV transactivation. Proc Natl Acad Sci U S A (2013) 1.04
Making the most of synthetic biology. Strategies for synthetic biology development in Europe. EMBO Rep (2009) 1.03
Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins. J Virol (2006) 1.03
In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na(+)-citrate symporter CitS as an example. J Struct Biol (2007) 1.03
Cyclic AMP regulation of protein lysine acetylation in Mycobacterium tuberculosis. Nat Struct Mol Biol (2012) 1.02
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J Mol Biol (2010) 1.02
Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. Proc Natl Acad Sci U S A (2012) 1.01
tRNA binding, structure, and localization of the human interferon-induced protein IFIT5. Mol Cell (2013) 1.01
Transfer of engineered biophysical properties between different antibody formats and expression systems. Protein Eng Des Sel (2012) 1.00