Published in Nat Biotechnol on May 18, 2003
Selection and evolution of enzymes from a partially randomized non-catalytic scaffold. Nature (2007) 2.51
Laboratory-directed protein evolution. Microbiol Mol Biol Rev (2005) 1.69
Chemically programmed antibodies. Trends Biotechnol (2014) 0.92
Mining human antibody repertoires. MAbs (2010) 0.92
Routes to covalent catalysis by reactive selection for nascent protein nucleophiles. J Am Chem Soc (2007) 0.90
In vitro affinity screening of protein and peptide binders by megavalent bead surface display. Protein Eng Des Sel (2013) 0.89
OptGraft: A computational procedure for transferring a binding site onto an existing protein scaffold. Protein Sci (2009) 0.87
Directed evolution strategies for improved enzymatic performance. Microb Cell Fact (2005) 0.85
Reactibodies generated by kinetic selection couple chemical reactivity with favorable protein dynamics. Proc Natl Acad Sci U S A (2011) 0.84
Synthesis and evaluation of general mechanism-based inhibitors of sulfatases based on (difluoro)methyl phenyl sulfate and cyclic phenyl sulfamate motifs. Bioorg Med Chem (2006) 0.82
Screening for a single-chain variable-fragment antibody that can effectively neutralize the cytotoxicity of the Vibrio parahaemolyticus thermolabile hemolysin. Appl Environ Microbiol (2012) 0.81
Development of a functional antibody by using a green fluorescent protein frame as the template. Appl Environ Microbiol (2014) 0.76
Robotic QM/MM-driven maturation of antibody combining sites. Sci Adv (2016) 0.75
Mechanism-dependent selection of immunoglobulin gene library for obtaining covalent biocatalysts. Dokl Biochem Biophys (2007) 0.75
Compartmentalized partnered replication for the directed evolution of genetic parts and circuits. Nat Protoc (2017) 0.75
The structure of H5N1 avian influenza neuraminidase suggests new opportunities for drug design. Nature (2006) 6.00
Structure and catalytic mechanism of the human histone methyltransferase SET7/9. Nature (2003) 4.00
Engineering novel binding proteins from nonimmunoglobulin domains. Nat Biotechnol (2005) 3.67
Kaposi sarcoma herpesvirus-induced cellular reprogramming contributes to the lymphatic endothelial gene expression in Kaposi sarcoma. Nat Genet (2004) 3.38
High-affinity binders selected from designed ankyrin repeat protein libraries. Nat Biotechnol (2004) 3.34
Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J Mol Biol (2003) 2.81
Crystal structure and functional analysis of the histone methyltransferase SET7/9. Cell (2002) 2.27
Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display. Nat Biotechnol (2006) 2.18
ProteomeBinders: planning a European resource of affinity reagents for analysis of the human proteome. Nat Methods (2007) 2.04
Designed to be stable: crystal structure of a consensus ankyrin repeat protein. Proc Natl Acad Sci U S A (2003) 1.98
Inhibition of caspase-2 by a designed ankyrin repeat protein: specificity, structure, and inhibition mechanism. Structure (2007) 1.93
Domain interactions in the Fab fragment: a comparative evaluation of the single-chain Fv and Fab format engineered with variable domains of different stability. J Mol Biol (2005) 1.90
A label-free immunosensor array using single-chain antibody fragments. Proc Natl Acad Sci U S A (2005) 1.85
Biophysical properties of human antibody variable domains. J Mol Biol (2003) 1.80
Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. Structure (2005) 1.75
A designed ankyrin repeat protein evolved to picomolar affinity to Her2. J Mol Biol (2007) 1.71
Associations between Burkitt lymphoma among children in Malawi and infection with HIV, EBV and malaria: results from a case-control study. PLoS One (2008) 1.70
In-vitro protein evolution by ribosome display and mRNA display. J Immunol Methods (2004) 1.69
Ribosome display: selecting and evolving proteins in vitro that specifically bind to a target. Nat Methods (2007) 1.68
Folding and unfolding mechanism of highly stable full-consensus ankyrin repeat proteins. J Mol Biol (2007) 1.67
Design of multivalent complexes using the barnase*barstar module. Nat Biotechnol (2003) 1.65
Direct identification of ligand-receptor interactions on living cells and tissues. Nat Biotechnol (2012) 1.64
Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. Proc Natl Acad Sci U S A (2008) 1.63
Evolution of three human GPCRs for higher expression and stability. J Mol Biol (2011) 1.62
DARPins and other repeat protein scaffolds: advances in engineering and applications. Curr Opin Biotechnol (2011) 1.61
Efficient selection of DARPins with sub-nanomolar affinities using SRP phage display. J Mol Biol (2008) 1.57
T-cell receptor gene transfer exclusively to human CD8(+) cells enhances tumor cell killing. Blood (2012) 1.55
Catalytic roles for carbon-oxygen hydrogen bonding in SET domain lysine methyltransferases. J Biol Chem (2006) 1.53
Efficient tumor targeting with high-affinity designed ankyrin repeat proteins: effects of affinity and molecular size. Cancer Res (2010) 1.51
KSHV-encoded miRNAs target MAF to induce endothelial cell reprogramming. Genes Dev (2010) 1.48
Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scFv) with low picomolar affinity. J Biol Chem (2004) 1.46
Development of a generic adenovirus delivery system based on structure-guided design of bispecific trimeric DARPin adapters. Proc Natl Acad Sci U S A (2013) 1.46
Designing repeat proteins: modular leucine-rich repeat protein libraries based on the mammalian ribonuclease inhibitor family. J Mol Biol (2003) 1.39
Stability improvement of antibodies for extracellular and intracellular applications: CDR grafting to stable frameworks and structure-based framework engineering. Methods (2004) 1.37
Short- and long-term effects of highly active antiretroviral therapy on Kaposi sarcoma-associated herpesvirus immune responses and viraemia. AIDS (2004) 1.34
Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells. Cancer Res (2007) 1.33
Designed armadillo repeat proteins as general peptide-binding scaffolds: consensus design and computational optimization of the hydrophobic core. J Mol Biol (2007) 1.33
Structure of a kinesin-tubulin complex and implications for kinesin motility. Nat Struct Mol Biol (2013) 1.31
Antibiotic recognition by binuclear metallo-beta-lactamases revealed by X-ray crystallography. J Am Chem Soc (2005) 1.30
A Burkholderia pseudomallei toxin inhibits helicase activity of translation factor eIF4A. Science (2011) 1.27
PEGylation and multimerization of the anti-p185HER-2 single chain Fv fragment 4D5: effects on tumor targeting. J Biol Chem (2006) 1.26
Direct in vivo screening of intrabody libraries constructed on a highly stable single-chain framework. J Biol Chem (2002) 1.25
Engineered proteins as specific binding reagents. Curr Opin Biotechnol (2005) 1.24
Biophysical properties of camelid V(HH) domains compared to those of human V(H)3 domains. Biochemistry (2002) 1.22
The crystal structure of diadenosine tetraphosphate hydrolase from Caenorhabditis elegans in free and binary complex forms. Structure (2002) 1.21
Folding of a designed simple ankyrin repeat protein. Protein Sci (2004) 1.20
Stepwise unfolding of ankyrin repeats in a single protein revealed by atomic force microscopy. Biophys J (2005) 1.20
Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: comparison of DARPin and camelid VHH binding mode. J Biol Chem (2009) 1.19
Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem (2005) 1.17
A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc Natl Acad Sci U S A (2012) 1.16
Selection and characterization of Her2 binding-designed ankyrin repeat proteins. J Biol Chem (2006) 1.15
A novel strategy to design binding molecules harnessing the modular nature of repeat proteins. FEBS Lett (2003) 1.15
Recombinant extracellular domains of tetraspanin proteins are potent inhibitors of the infection of macrophages by human immunodeficiency virus type 1. J Virol (2006) 1.15
Structure of signaling-competent neurotensin receptor 1 obtained by directed evolution in Escherichia coli. Proc Natl Acad Sci U S A (2014) 1.15
Protein PEGylation decreases observed target association rates via a dual blocking mechanism. Mol Pharmacol (2005) 1.14
A recombinant immunotoxin derived from a humanized epithelial cell adhesion molecule-specific single-chain antibody fragment has potent and selective antitumor activity. Clin Cancer Res (2003) 1.13
ATP analogues with modified phosphate chains and their selectivity for rat P2X2 and P2X2/3 receptors. Br J Pharmacol (2003) 1.12
Transition state analogue structures of human phosphoglycerate kinase establish the importance of charge balance in catalysis. J Am Chem Soc (2010) 1.09
Critical features for biosynthesis, stability, and functionality of a G protein-coupled receptor uncovered by all-versus-all mutations. Proc Natl Acad Sci U S A (2012) 1.09
Affinity-matured recombinant antibody fragments analyzed by single-molecule force spectroscopy. Biophys J (2007) 1.09
DARPins: an efficient targeting domain for lentiviral vectors. Mol Ther (2011) 1.08
Structure-based improvement of the biophysical properties of immunoglobulin VH domains with a generalizable approach. Biochemistry (2003) 1.06
Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation. J Mol Biol (2006) 1.06
Designed ankyrin repeat proteins (DARPins) from research to therapy. Methods Enzymol (2012) 1.06
In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: The Na(+)-citrate symporter CitS as an example. J Struct Biol (2007) 1.03
LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing. Proc Natl Acad Sci U S A (2010) 1.03
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J Mol Biol (2010) 1.02
KSHV manipulates Notch signaling by DLL4 and JAG1 to alter cell cycle genes in lymphatic endothelia. PLoS Pathog (2009) 1.02
Characterisation of receptor binding by the chemotaxis inhibitory protein of Staphylococcus aureus and the effects of the host immune response. Mol Immunol (2007) 1.01
Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. Proc Natl Acad Sci U S A (2012) 1.01
Transfer of engineered biophysical properties between different antibody formats and expression systems. Protein Eng Des Sel (2012) 1.00
Bispecific designed ankyrin repeat proteins (DARPins) targeting epidermal growth factor receptor inhibit A431 cell proliferation and receptor recycling. J Biol Chem (2011) 1.00
A Trojan horse transition state analogue generated by MgF3- formation in an enzyme active site. Proc Natl Acad Sci U S A (2006) 1.00
An antibody library for stabilizing and crystallizing membrane proteins - selecting binders to the citrate carrier CitS. FEBS Lett (2004) 1.00
Stabilizing ionic interactions in a full-consensus ankyrin repeat protein. J Mol Biol (2007) 0.99
Biochemical, crystallographic, and mutagenic characterization of hint, the AMP-lysine hydrolase, with novel substrates and inhibitors. J Biol Chem (2004) 0.99
EpCAM-targeted delivery of nanocomplexed siRNA to tumor cells with designed ankyrin repeat proteins. Mol Cancer Ther (2009) 0.99
Consensus design of repeat proteins. Chembiochem (2004) 0.98
Structural basis for eliciting a cytotoxic effect in HER2-overexpressing cancer cells via binding to the extracellular domain of HER2. Structure (2013) 0.98
Structural requirements for the inhibitory action of the CD9 large extracellular domain in sperm/oocyte binding and fusion. Biochem Biophys Res Commun (2003) 0.97
Anionic charge is prioritized over geometry in aluminum and magnesium fluoride transition state analogs of phosphoryl transfer enzymes. J Am Chem Soc (2008) 0.97
DARPins recognizing the tumor-associated antigen EpCAM selected by phage and ribosome display and engineered for multivalency. J Mol Biol (2011) 0.97
Antitumor activity of an epithelial cell adhesion molecule targeted nanovesicular drug delivery system. Mol Cancer Ther (2007) 0.97
Protein tag-mediated conjugation of oligonucleotides to recombinant affinity binders for proximity ligation. N Biotechnol (2012) 0.97
Direct molecular evolution of detergent-stable G protein-coupled receptors using polymer encapsulated cells. J Mol Biol (2012) 0.97
Stabilizing membrane proteins through protein engineering. Curr Opin Chem Biol (2013) 0.96
Maximizing detergent stability and functional expression of a GPCR by exhaustive recombination and evolution. J Mol Biol (2012) 0.96
Atomic details of near-transition state conformers for enzyme phosphoryl transfer revealed by MgF-3 rather than by phosphoranes. Proc Natl Acad Sci U S A (2010) 0.96
Isolation of intracellular proteinase inhibitors derived from designed ankyrin repeat proteins by genetic screening. J Biol Chem (2006) 0.95
Comparison of in vivo selection and rational design of heterodimeric coiled coils. Structure (2002) 0.95