Published in Methods Mol Biol on January 01, 2003
Incorporation of non-natural amino acids into proteins. Curr Opin Chem Biol (2002) 1.50
Farnesyl pyrophosphate regulates adipocyte functions as an endogenous PPARγ agonist. Biochem J (2011) 1.49
FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids. Nat Methods (2006) 1.31
Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis system. J Am Chem Soc (2007) 1.10
Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs. Nat Biotechnol (2002) 1.03
Spatial regulation of specific gene expression through photoactivation of RNAi. J Control Release (2009) 0.98
Multiple incorporation of non-natural amino acids into a single protein using tRNAs with non-standard structures. FEBS Lett (2005) 0.97
"Quenchbodies": quench-based antibody probes that show antigen-dependent fluorescence. J Am Chem Soc (2011) 0.96
Photoswitching of peroxidase activity by position-specific incorporation of a photoisomerizable non-natural amino acid into horseradish peroxidase. FEBS Lett (2002) 0.96
Position-specific incorporation of dansylated non-natural amino acids into streptavidin by using a four-base codon. FEBS Lett (2004) 0.95
Four-base codon-mediated incorporation of non-natural amino acids into proteins in a eukaryotic cell-free translation system. J Biosci Bioeng (2005) 0.94
Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs. J Am Chem Soc (2002) 0.92
Cellular siRNA delivery mediated by a cell-permeant RNA-binding protein and photoinduced RNA interference. Bioconjug Chem (2008) 0.88
Position-specific incorporation of a highly photodurable and blue-laser excitable fluorescent amino acid into proteins for fluorescence sensing. Bioorg Med Chem (2005) 0.87
N-terminal specific point-immobilization of active proteins by the one-pot NEXT-A method. Chembiochem (2009) 0.87
Incorporation of fluorescent non-natural amino acids into N-terminal tag of proteins in cell-free translation and its dependence on position and neighboring codons. J Biosci Bioeng (2010) 0.86
An evolutionary 'intermediate state' of mitochondrial translation systems found in Trichinella species of parasitic nematodes: co-evolution of tRNA and EF-Tu. Nucleic Acids Res (2006) 0.85
Design of carrier tRNAs and selection of four-base codons for efficient incorporation of various nonnatural amino acids into proteins in Spodoptera frugiperda 21 (Sf21) insect cell-free translation system. J Biosci Bioeng (2006) 0.85
Leucyl/phenylalanyl(L/F)-tRNA-protein transferase-mediated aminoacyl transfer of a nonnatural amino acid to the N-terminus of peptides and proteins and subsequent functionalization by bioorthogonal reactions. Biopolymers (2007) 0.84
Incorporation of fluorescently labeled nonnatural amino acids into proteins in an E. coli in vitro translation system. Nucleic Acids Res Suppl (2003) 0.82
Four-base codon/anticodon strategy and non-enzymatic aminoacylation for protein engineering with non-natural amino acids. Methods (2005) 0.82
Expanding the genetic code in a mammalian cell line by the introduction of four-base codon/anticodon pairs. Chembiochem (2006) 0.82
Chemoenzymatic transfer of fluorescent non-natural amino acids to the N terminus of a protein/peptide. Chembiochem (2008) 0.81
Leucyl/Phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids. Chembiochem (2006) 0.81
Use of EF-Tu mutants for determining and improving aminoacylation efficiency and for purifying aminoacyl tRNAs with non-natural amino acids. J Biochem (2010) 0.80
Position-specific incorporation of fluorescent non-natural amino acids into maltose-binding protein for detection of ligand binding by FRET and fluorescence quenching. Chembiochem (2009) 0.80
Site-specific incorporation of PEGylated amino acids into proteins using nonnatural amino acid mutagenesis. Bioorg Med Chem Lett (2009) 0.79
Position-specific incorporation of biotinylated non-natural amino acids into a protein in a cell-free translation system. Biochem Biophys Res Commun (2007) 0.79
Four-base codon-mediated saturation mutagenesis in a cell-free translation system. J Biosci Bioeng (2008) 0.79
The NEXT-A (N-terminal EXtension with Transferase and ARS) reaction. Nucleic Acids Symp Ser (Oxf) (2009) 0.79
Development of amber suppressor tRNAs appropriate for incorporation of nonnatural amino acids. Nucleic Acids Symp Ser (Oxf) (2006) 0.79
Binding efficiency of elongation factor Tu to tRNAs charged with nonnatural fluorescent amino acids. Anal Biochem (2005) 0.79
Photo-dependent protein biosynthesis using a caged aminoacyl-tRNA. Bioorg Med Chem Lett (2014) 0.79
Comprehensive screening of amber suppressor tRNAs suitable for incorporation of non-natural amino acids in a cell-free translation system. Biochem Biophys Res Commun (2008) 0.79
Synthesis of a cyclic peptide/protein using the NEXT-A reaction followed by cyclization. Chem Commun (Camb) (2011) 0.79
In situ chemical aminoacylation with amino acid thioesters linked to a peptide nucleic acid. J Am Chem Soc (2004) 0.79
Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids. Nucleic Acids Res (2006) 0.79
Site-specific incorporation of arginine analogs into proteins using arginyl-tRNA synthetase. Biochem Biophys Res Commun (2011) 0.79
N-terminal specific fluorescence labeling of proteins through incorporation of fluorescent hydroxy acid and subsequent ester cleavage. Chembiochem (2008) 0.78
Detection of bioactive small molecules by fluorescent resonance energy transfer (FRET) in RNA-protein conjugates. Bioconjug Chem (2009) 0.78
Photo inducible RNA interference using cell permeable protein carrier. Nucleic Acids Symp Ser (Oxf) (2007) 0.78
Lactobacillus-mediated RNA interference in nematode. J Biosci Bioeng (2009) 0.78
Efficient synthesis of nonnatural mutants in Escherichia coli S30 in vitro protein synthesizing system. J Biosci Bioeng (2004) 0.78
In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system. Nucleic Acids Res (2006) 0.78
Simple and quick chemical aminoacylation of tRNA in cationic micellar solution under ultrasonic agitation. Chem Commun (Camb) (2005) 0.78
A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates. Anal Biochem (2009) 0.77
Molecular design of glycoprotein mimetics: glycoblotting by engineered proteins with an oxylamino-functionalized amino acid residue. Chemistry (2005) 0.77
Carrier PNA for shRNA delivery into cells. Bioorg Med Chem Lett (2009) 0.77
Facile aminoacylation of pdCpA dinucleotide with a nonnatural amino acid in cationic micelle. Chem Commun (Camb) (2003) 0.77
A small-molecule-based approach to sense codon-templated natural-unnatural hybrid peptides. Selective silencing and reassignment of the sense codon by orthogonal reacylation stalling at the single-codon level. J Am Chem Soc (2005) 0.76
pH-sensitive locomotion of cyclodextrins in a block-selective mobile polyrotaxane. Chemphyschem (2006) 0.75
Incorporation of anthraquinonyl group into lambda-Cro repressor protein for strand- and position-specific photocleavage of double-stranded DNA. Biopolymers (2004) 0.75
Incorporation of unnatural non-alpha-amino acids into the N terminus of proteins in a cell-free translation system. Chembiochem (2007) 0.75
Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli. Bioorg Med Chem Lett (2010) 0.75
Screening of amber suppressor tRNAs suitable to introduce nonnatural amino acids into proteins by real-time monitoring of cell-free translation. Nucleic Acids Symp Ser (Oxf) (2009) 0.75
Biosynthesis of proteins containing modified lysines and fluorescent labels using non-natural amino acid mutagenesis. J Biosci Bioeng (2011) 0.75
N-terminal specific fluorescence labeling of proteins through fourbase codon-mediated incorporation of fluorescent hydroxy acid. Nucleic Acids Symp Ser (Oxf) (2007) 0.75
Nonnatural mutagenesis in E. coli and rabbit reticulocyte lysates by using four-base codons. Nucleic Acids Res Suppl (2002) 0.75
Configurational preference of pyrrolidine-based oxy-peptide nucleic acids as hybridization counterparts with DNA and RNA. Biopolymers (2006) 0.75
Oxy-peptide nucleic acid with a pyrrolidine ring that is configurationally optimized for hybridization with DNA. Chem Commun (Camb) (2004) 0.75
Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position. Protein Eng Des Sel (2005) 0.75
Incorporation of glycosylated amino acid into protein by an in vitro translation system. Bioorg Med Chem Lett (2013) 0.75
Thermodynamic analysis of hybridization between POPNA and DNA. Nucleic Acids Res Suppl (2002) 0.75
Quantitative screening of EGF receptor-binding peptides by using a peptide library with multiple fluorescent amino acids as fluorescent tags. Bioorg Med Chem Lett (2010) 0.75
Aminoacylation of tRNA by antisense molecule. Nucleic Acids Res Suppl (2002) 0.75
Delivery of dsRNA with lactic acid bacteria for RNA interference. Nucleic Acids Symp Ser (Oxf) (2007) 0.75
Preparation of glycosylated amino acid derivatives for glycoprotein synthesis by in vitro translation system. Bioorg Med Chem (2002) 0.75
RNA isolation using immobilized PNA. Nucleic Acids Symp Ser (Oxf) (2005) 0.75
A novel method for screening peptides that bind to proteins by using multiple fluorescent amino acids as fluorescent tags. Chem Commun (Camb) (2009) 0.75
Synthesis of a newly peptide nucleic acid that contains tertiary amino groups. Nucleic Acids Symp Ser (Oxf) (2005) 0.75
Amber codon-mediated expanded saturation mutagenesis of proteins using a cell-free translation system. J Biosci Bioeng (2012) 0.75
Synthesis of pyrrolidine-based oxy-peptide nucleic acids that contain four bases and their properties. Nucleic Acids Symp Ser (Oxf) (2004) 0.75
A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin. Nucleic Acids Res Suppl (2002) 0.75
Efficient incorporation of a nonnatural amino acid into a protein in an insect cell-free translation system. Nucleic Acids Symp Ser (Oxf) (2006) 0.75
Isolation of small RNAs using biotinylated PNAs. J Biochem (2008) 0.75
Site-directed incorporation of non-natural amino acid into Streptomyces xylanase. Nucleic Acids Symp Ser (Oxf) (2004) 0.75