RNA isolation using immobilized PNA.

PubWeight™: 0.75‹?›

🔗 View Article (PMID 17150734)

Published in Nucleic Acids Symp Ser (Oxf) on January 01, 2005

Authors

Takashi Ohtsuki1, Chisato Kumano, Taishi Manabe, Mizuki Kitamatsu, Masahiko Sisido

Author Affiliations

1: Department of Bioscience and Biotechnology, Okayama University, 3-1-1 Tsushimanaka, Okayama 700-8530, Japan.

Articles by these authors

Incorporation of non-natural amino acids into proteins. Curr Opin Chem Biol (2002) 1.50

FRET analysis of protein conformational change through position-specific incorporation of fluorescent amino acids. Nat Methods (2006) 1.31

Elongation factor Tu mutants expand amino acid tolerance of protein biosynthesis system. J Am Chem Soc (2007) 1.10

Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs. Nat Biotechnol (2002) 1.03

Spatial regulation of specific gene expression through photoactivation of RNAi. J Control Release (2009) 0.98

Multiple incorporation of non-natural amino acids into a single protein using tRNAs with non-standard structures. FEBS Lett (2005) 0.97

Photoswitching of peroxidase activity by position-specific incorporation of a photoisomerizable non-natural amino acid into horseradish peroxidase. FEBS Lett (2002) 0.96

Position-specific incorporation of dansylated non-natural amino acids into streptavidin by using a four-base codon. FEBS Lett (2004) 0.95

Four-base codon-mediated incorporation of non-natural amino acids into proteins in a eukaryotic cell-free translation system. J Biosci Bioeng (2005) 0.94

Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs. J Am Chem Soc (2002) 0.92

Cellular siRNA delivery mediated by a cell-permeant RNA-binding protein and photoinduced RNA interference. Bioconjug Chem (2008) 0.88

Position-specific incorporation of a highly photodurable and blue-laser excitable fluorescent amino acid into proteins for fluorescence sensing. Bioorg Med Chem (2005) 0.87

N-terminal specific point-immobilization of active proteins by the one-pot NEXT-A method. Chembiochem (2009) 0.87

An evolutionary 'intermediate state' of mitochondrial translation systems found in Trichinella species of parasitic nematodes: co-evolution of tRNA and EF-Tu. Nucleic Acids Res (2006) 0.85

Design of carrier tRNAs and selection of four-base codons for efficient incorporation of various nonnatural amino acids into proteins in Spodoptera frugiperda 21 (Sf21) insect cell-free translation system. J Biosci Bioeng (2006) 0.85

Leucyl/phenylalanyl(L/F)-tRNA-protein transferase-mediated aminoacyl transfer of a nonnatural amino acid to the N-terminus of peptides and proteins and subsequent functionalization by bioorthogonal reactions. Biopolymers (2007) 0.84

A protein transduction method using oligo-arginine (3R) for the delivery of transcription factors into cell nuclei. Biomaterials (2012) 0.83

Incorporation of fluorescently labeled nonnatural amino acids into proteins in an E. coli in vitro translation system. Nucleic Acids Res Suppl (2003) 0.82

Expanding the genetic code in a mammalian cell line by the introduction of four-base codon/anticodon pairs. Chembiochem (2006) 0.82

Leucyl/Phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids. Chembiochem (2006) 0.81

Chemoenzymatic transfer of fluorescent non-natural amino acids to the N terminus of a protein/peptide. Chembiochem (2008) 0.81

Use of EF-Tu mutants for determining and improving aminoacylation efficiency and for purifying aminoacyl tRNAs with non-natural amino acids. J Biochem (2010) 0.80

Site-specific incorporation of arginine analogs into proteins using arginyl-tRNA synthetase. Biochem Biophys Res Commun (2011) 0.79

Binding efficiency of elongation factor Tu to tRNAs charged with nonnatural fluorescent amino acids. Anal Biochem (2005) 0.79

Photo-dependent protein biosynthesis using a caged aminoacyl-tRNA. Bioorg Med Chem Lett (2014) 0.79

Synthesis of a cyclic peptide/protein using the NEXT-A reaction followed by cyclization. Chem Commun (Camb) (2011) 0.79

The NEXT-A (N-terminal EXtension with Transferase and ARS) reaction. Nucleic Acids Symp Ser (Oxf) (2009) 0.79

In situ chemical aminoacylation with amino acid thioesters linked to a peptide nucleic acid. J Am Chem Soc (2004) 0.79

Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids. Nucleic Acids Res (2006) 0.79

Photo inducible RNA interference using cell permeable protein carrier. Nucleic Acids Symp Ser (Oxf) (2007) 0.78

In vitro selection of tRNAs for efficient four-base decoding to incorporate non-natural amino acids into proteins in an Escherichia coli cell-free translation system. Nucleic Acids Res (2006) 0.78

Detection of bioactive small molecules by fluorescent resonance energy transfer (FRET) in RNA-protein conjugates. Bioconjug Chem (2009) 0.78

Three wavelength substrate system of neutrophil serine proteinases. Anal Chem (2012) 0.78

Efficient synthesis of nonnatural mutants in Escherichia coli S30 in vitro protein synthesizing system. J Biosci Bioeng (2004) 0.78

Simple and quick chemical aminoacylation of tRNA in cationic micellar solution under ultrasonic agitation. Chem Commun (Camb) (2005) 0.78

Lactobacillus-mediated RNA interference in nematode. J Biosci Bioeng (2009) 0.78

Random insertion and deletion mutagenesis. Methods Mol Biol (2003) 0.77

A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates. Anal Biochem (2009) 0.77

Facile aminoacylation of pdCpA dinucleotide with a nonnatural amino acid in cationic micelle. Chem Commun (Camb) (2003) 0.77

Carrier PNA for shRNA delivery into cells. Bioorg Med Chem Lett (2009) 0.77

Incorporation of anthraquinonyl group into lambda-Cro repressor protein for strand- and position-specific photocleavage of double-stranded DNA. Biopolymers (2004) 0.75

Aminoacylation of tRNA by antisense molecule. Nucleic Acids Res Suppl (2002) 0.75

Antisense effect of pyrrolidine-based oxy-peptide nucleic acids in Escherichia coli. Bioorg Med Chem Lett (2010) 0.75

Circularly polarised luminescence and circular dichroism of l- and d-oligopeptides with multiple pyrenes. Org Biomol Chem (2015) 0.75

Delivery of dsRNA with lactic acid bacteria for RNA interference. Nucleic Acids Symp Ser (Oxf) (2007) 0.75

Synthesis and sequence optimization of GFP mutants containing aromatic non-natural amino acids at the Tyr66 position. Protein Eng Des Sel (2005) 0.75

Thermodynamic analysis of hybridization between POPNA and DNA. Nucleic Acids Res Suppl (2002) 0.75

Preparation of glycosylated amino acid derivatives for glycoprotein synthesis by in vitro translation system. Bioorg Med Chem (2002) 0.75

A novel method for screening peptides that bind to proteins by using multiple fluorescent amino acids as fluorescent tags. Chem Commun (Camb) (2009) 0.75

Synthesis of a newly peptide nucleic acid that contains tertiary amino groups. Nucleic Acids Symp Ser (Oxf) (2005) 0.75

Synthesis of pyrrolidine-based oxy-peptide nucleic acids that contain four bases and their properties. Nucleic Acids Symp Ser (Oxf) (2004) 0.75

On the preparation of indoxyl red from indican and some new characteristics. Bioorg Med Chem Lett (2012) 0.75

Isolation of small RNAs using biotinylated PNAs. J Biochem (2008) 0.75

Efficient incorporation of a nonnatural amino acid into a protein in an insect cell-free translation system. Nucleic Acids Symp Ser (Oxf) (2006) 0.75

Nonnatural mutagenesis in E. coli and rabbit reticulocyte lysates by using four-base codons. Nucleic Acids Res Suppl (2002) 0.75

Configurational preference of pyrrolidine-based oxy-peptide nucleic acids as hybridization counterparts with DNA and RNA. Biopolymers (2006) 0.75

[Cellular RNA delivery using carrier peptides]. Seikagaku (2009) 0.75

Oxy-peptide nucleic acid with a pyrrolidine ring that is configurationally optimized for hybridization with DNA. Chem Commun (Camb) (2004) 0.75

Site-directed incorporation of non-natural amino acid into Streptomyces xylanase. Nucleic Acids Symp Ser (Oxf) (2004) 0.75

A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin. Nucleic Acids Res Suppl (2002) 0.75

Quantitative screening of EGF receptor-binding peptides by using a peptide library with multiple fluorescent amino acids as fluorescent tags. Bioorg Med Chem Lett (2010) 0.75