Published in J Cell Biol on December 01, 1992
Force generation by microtubule assembly/disassembly in mitosis and related movements. Mol Biol Cell (1995) 5.78
Microtubules orient the mitotic spindle in yeast through dynein-dependent interactions with the cell cortex. J Cell Biol (1997) 5.08
Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling. J Cell Biol (1997) 4.55
Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol Biol Cell (1997) 3.05
XMAP from Xenopus eggs promotes rapid plus end assembly of microtubules and rapid microtubule polymer turnover. J Cell Biol (1994) 2.50
Cyclin B interaction with microtubule-associated protein 4 (MAP4) targets p34cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. J Cell Biol (1995) 2.35
A novel pool of protein phosphatase 2A is associated with microtubules and is regulated during the cell cycle. J Cell Biol (1995) 2.02
Evidence that a single monolayer tubulin-GTP cap is both necessary and sufficient to stabilize microtubules. Mol Biol Cell (1996) 1.77
Dissociation of the tubulin-sequestering and microtubule catastrophe-promoting activities of oncoprotein 18/stathmin. Mol Biol Cell (1999) 1.66
Vinblastine suppresses dynamics of individual microtubules in living interphase cells. Mol Biol Cell (1995) 1.66
Protein phosphatase 1 regulates the cytoplasmic dynein-driven formation of endoplasmic reticulum networks in vitro. J Cell Biol (1995) 1.58
Kinetics of microtubule catastrophe assessed by probabilistic analysis. Biophys J (1995) 1.49
Identification of XMAP215 as a microtubule-destabilizing factor in Xenopus egg extract by biochemical purification. J Cell Biol (2003) 1.49
Dynamic instability of microtubules: Monte Carlo simulation and application to different types of microtubule lattice. Biophys J (1993) 1.45
How the transition frequencies of microtubule dynamic instability (nucleation, catastrophe, and rescue) regulate microtubule dynamics in interphase and mitosis: analysis using a Monte Carlo computer simulation. Mol Biol Cell (1993) 1.43
Cytoplasmic dynein undergoes intracellular redistribution concomitant with phosphorylation of the heavy chain in response to serum starvation and okadaic acid. J Cell Biol (1994) 1.37
The C. elegans RSA complex localizes protein phosphatase 2A to centrosomes and regulates mitotic spindle assembly. Cell (2007) 1.34
A metastable intermediate state of microtubule dynamic instability that differs significantly between plus and minus ends. J Cell Biol (1997) 1.27
Kif18A uses a microtubule binding site in the tail for plus-end localization and spindle length regulation. Curr Biol (2011) 1.26
Intrinsic microtubule stability in interphase cells. J Cell Biol (1994) 1.25
Effect on microtubule dynamics of XMAP230, a microtubule-associated protein present in Xenopus laevis eggs and dividing cells. J Cell Biol (1994) 1.23
MCAK and paclitaxel have differential effects on spindle microtubule organization and dynamics. Mol Biol Cell (2009) 1.01
Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos. Development (2010) 0.98
In situ imaging in C. elegans reveals developmental regulation of microtubule dynamics. Dev Cell (2014) 0.86
Protein tyrosine and serine-threonine phosphatases in the sea urchin, Strongylocentrotus purpuratus: identification and potential functions. Dev Biol (2006) 0.84
Cytoskeletal reorganization of human platelets induced by the protein phosphatase 1/2 A inhibitors okadaic acid and calyculin A. Biochem J (1995) 0.82
LB100, a small molecule inhibitor of PP2A with potent chemo- and radio-sensitizing potential. Cancer Biol Ther (2015) 0.79
Equilibria of idealized confined astral microtubules and coupled spindle poles. PLoS One (2012) 0.75
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A (1979) 365.71
Cyclin: a protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division. Cell (1983) 9.90
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. J Cell Biol (1988) 9.32
Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem J (1988) 8.02
Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching. J Cell Biol (1984) 5.23
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts. Cell (1990) 4.92
Rapid stimulation by insulin of a serine/threonine kinase in 3T3-L1 adipocytes that phosphorylates microtubule-associated protein 2 in vitro. Proc Natl Acad Sci U S A (1987) 4.61
Effects of the tumour promoter okadaic acid on intracellular protein phosphorylation and metabolism. Nature (1989) 4.27
Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs. Nature (1990) 3.77
In vitro effects on microtubule dynamics of purified Xenopus M phase-activated MAP kinase. Nature (1991) 3.59
Fission yeast p13 blocks mitotic activation and tyrosine dephosphorylation of the Xenopus cdc2 protein kinase. Cell (1989) 3.56
Extracellular signal-regulated kinases: ERKs in progress. Cell Regul (1991) 3.47
Direct observation of microtubule dynamics in living cells. Nature (1988) 3.47
Real-time observations of microtubule dynamic instability in living cells. J Cell Biol (1988) 3.46
Kinetochores capture astral microtubules during chromosome attachment to the mitotic spindle: direct visualization in live newt lung cells. J Cell Biol (1990) 3.38
An improved procedure for identifying and quantitating protein phosphatases in mammalian tissues. FEBS Lett (1989) 3.10
Taxol-induced microtubule asters in mitotic extracts of Xenopus eggs: requirement for phosphorylated factors and cytoplasmic dynein. J Cell Biol (1991) 2.90
Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes. J Cell Biol (1985) 2.72
Xenopus M phase MAP kinase: isolation of its cDNA and activation by MPF. EMBO J (1991) 2.68
Severing of stable microtubules by a mitotically activated protein in Xenopus egg extracts. Cell (1991) 2.66
New features of microtubule behaviour observed in vivo. Nature (1988) 2.35
Dynamics of microtubule depolymerization in monocytes. J Cell Biol (1986) 2.01
Use and specificity of staurosporine, UCN-01, and calphostin C as protein kinase inhibitors. Methods Enzymol (1991) 2.01
Microtubule dynamic instability: numerical simulation of microtubule transition properties using a Lateral Cap model. J Cell Sci (1990) 1.84
Microinjection of p34cdc2 kinase induces marked changes in cell shape, cytoskeletal organization, and chromatin structure in mammalian fibroblasts. Cell (1990) 1.82
Okadaic acid, a specific protein phosphatase inhibitor, induces maturation and MPF formation in Xenopus laevis oocytes. FEBS Lett (1989) 1.79
Aster formation in eggs of Xenopus laevis. Induction by isolated basal bodies. J Cell Biol (1975) 1.73
Okadaic acid mimics the action of insulin in stimulating protein kinase activity in isolated adipocytes. The role of protein phosphatase 2a in attenuation of the signal. J Biol Chem (1990) 1.68
Microtubule assembly in cytoplasmic extracts of Xenopus oocytes and eggs. J Cell Biol (1987) 1.67
Involvement of protein phosphatases 1 and 2A in the control of M phase-promoting factor activity in starfish. J Cell Biol (1989) 1.66
Okadaic acid activates microtubule-associated protein kinase in quiescent fibroblastic cells. Eur J Biochem (1990) 1.53
Incorporation of radioactive tubulin into microtubules at steady state. Experimental and theoretical analyses of diffusional and directional flux. J Biol Chem (1980) 1.48
Buffer conditions and non-tubulin factors critically affect the microtubule dynamic instability of sea urchin egg tubulin. Cell Motil Cytoskeleton (1992) 1.35
Activation of myelin basic protein kinases during echinoderm oocyte maturation and egg fertilization. Dev Biol (1988) 1.26
A simple formulation of microtubule dynamics: quantitative implications of the dynamic instability of microtubule populations in vivo and in vitro. J Cell Sci (1989) 1.20
Inhibition of mitosis by okadaic acid: possible involvement of a protein phosphatase 2A in the transition from metaphase to anaphase. J Cell Sci (1992) 1.18
Techniques for observing living gametes and embryos. Methods Cell Biol (1986) 1.13
Removal of the fertilization membrane of sea urchin embryos employing aminotriazole. Exp Cell Res (1979) 0.99
Structure-activity relationship within a series of okadaic acid derivatives. Carcinogenesis (1990) 0.97
Okadaic acid stimulates the activity of microtubule associated protein kinase in PC-12 pheochromocytoma cells. Biochem Biophys Res Commun (1990) 0.90
Protein phosphatases are involved in the in vivo activation of histone H1 kinase in mouse oocyte. Dev Biol (1990) 0.89
Unidirectional microtubule assembly in cell-free extracts of Spisula solidissima oocytes is regulated by subtle changes in pH. Cell Motil Cytoskeleton (1991) 0.87
The roles of macromolecular synthesis and phosphorylation in the regulation of a protein kinase activity transiently stimulated by nerve growth factor. J Biol Chem (1991) 0.87
A requirement for protein phosphorylation in regulating the meiotic and mitotic cell cycles in echinoderms. Dev Biol (1989) 0.83
Okadaic acid suppresses calcium regulation of mitosis onset in sea urchin embryos. Cell Regul (1991) 0.80
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies. J Cell Biol (1988) 9.32
Localization of Mad2 to kinetochores depends on microtubule attachment, not tension. J Cell Biol (1998) 6.49
Tubulin dynamics in cultured mammalian cells. J Cell Biol (1984) 6.46
Kinetochore microtubule dynamics and attachment stability are regulated by Hec1. Cell (2006) 6.46
Directional instability of kinetochore motility during chromosome congression and segregation in mitotic newt lung cells: a push-pull mechanism. J Cell Biol (1993) 6.18
Association of spindle assembly checkpoint component XMAD2 with unattached kinetochores. Science (1996) 6.10
The vertebrate cell kinetochore and its roles during mitosis. Trends Cell Biol (1998) 5.87
Force generation by microtubule assembly/disassembly in mitosis and related movements. Mol Biol Cell (1995) 5.78
Fluorescent speckle microscopy, a method to visualize the dynamics of protein assemblies in living cells. Curr Biol (1998) 5.69
Spindle dynamics and cell cycle regulation of dynein in the budding yeast, Saccharomyces cerevisiae. J Cell Biol (1995) 5.27
Spindle microtubule dynamics in sea urchin embryos: analysis using a fluorescein-labeled tubulin and measurements of fluorescence redistribution after laser photobleaching. J Cell Biol (1984) 5.23
Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cells. J Cell Biol (2001) 5.09
Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation. J Cell Biol (2001) 5.03
Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores. Mol Biol Cell (2001) 4.71
Chromosomes can congress to the metaphase plate before biorientation. Science (2006) 4.64
Motile kinetochores and polar ejection forces dictate chromosome position on the vertebrate mitotic spindle. J Cell Biol (1994) 4.56
Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling. J Cell Biol (1997) 4.55
The Drosophila claret segregation protein is a minus-end directed motor molecule. Nature (1990) 4.50
Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis. J Cell Biol (1992) 4.27
Oscillatory movements of monooriented chromosomes and their position relative to the spindle pole result from the ejection properties of the aster and half-spindle. J Cell Biol (1986) 4.26
Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cells. J Cell Biol (2000) 4.26
Microtubule growth activates Rac1 to promote lamellipodial protrusion in fibroblasts. Nat Cell Biol (1999) 4.08
The dynamic kinetochore-microtubule interface. J Cell Sci (2004) 4.07
Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis. J Cell Biol (1998) 4.07
Aurora kinase promotes turnover of kinetochore microtubules to reduce chromosome segregation errors. Curr Biol (2006) 3.96
The polarity and dynamics of microtubule assembly in the budding yeast Saccharomyces cerevisiae. Nat Cell Biol (2000) 3.76
hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J Cell Biol (2002) 3.69
Protein architecture of the human kinetochore microtubule attachment site. Cell (2009) 3.68
Spindle checkpoint protein dynamics at kinetochores in living cells. Curr Biol (2004) 3.67
Astral microtubule dynamics in yeast: a microtubule-based searching mechanism for spindle orientation and nuclear migration into the bud. J Cell Biol (1997) 3.65
Yeast Kar3 is a minus-end microtubule motor protein that destabilizes microtubules preferentially at the minus ends. EMBO J (1994) 3.63
Hec1 and nuf2 are core components of the kinetochore outer plate essential for organizing microtubule attachment sites. Mol Biol Cell (2004) 3.52
CENP-E function at kinetochores is essential for chromosome alignment. J Cell Biol (1997) 3.49
Real-time observations of microtubule dynamic instability in living cells. J Cell Biol (1988) 3.46
Endoplasmic reticulum membrane tubules are distributed by microtubules in living cells using three distinct mechanisms. Curr Biol (1998) 3.44
Budding yeast chromosome structure and dynamics during mitosis. J Cell Biol (2001) 3.39
The kinetochore microtubule minus-end disassembly associated with poleward flux produces a force that can do work. Mol Biol Cell (1996) 3.16
The human SWI/SNF-B chromatin-remodeling complex is related to yeast rsc and localizes at kinetochores of mitotic chromosomes. Proc Natl Acad Sci U S A (2000) 3.16
Localization and anchoring of mRNA in budding yeast. Curr Biol (1999) 3.12
Rapid assembly dynamics of the Escherichia coli FtsZ-ring demonstrated by fluorescence recovery after photobleaching. Proc Natl Acad Sci U S A (2002) 3.06
EB1-microtubule interactions in Xenopus egg extracts: role of EB1 in microtubule stabilization and mechanisms of targeting to microtubules. Mol Biol Cell (2002) 2.92
Merotelic kinetochore orientation occurs frequently during early mitosis in mammalian tissue cells and error correction is achieved by two different mechanisms. J Cell Sci (2003) 2.84
Oscillating mitotic newt lung cell kinetochores are, on average, under tension and rarely push. J Cell Sci (1996) 2.81
Determining the position of the cell division plane. Nature (2003) 2.80
Analysis of the treadmilling model during metaphase of mitosis using fluorescence redistribution after photobleaching. J Cell Biol (1986) 2.67
Mitosis: a history of division. Nat Cell Biol (2001) 2.65
In vivo protein architecture of the eukaryotic kinetochore with nanometer scale accuracy. Curr Biol (2009) 2.52
Stable kinetochore-microtubule attachment constrains centromere positioning in metaphase. Curr Biol (2004) 2.48
Traction force on a kinetochore at metaphase acts as a linear function of kinetochore fiber length. J Cell Biol (1982) 2.44
Dilution of individual microtubules observed in real time in vitro: evidence that cap size is small and independent of elongation rate. J Cell Biol (1991) 2.44
Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. Curr Biol (2003) 2.44
Rapid rate of tubulin dissociation from microtubules in the mitotic spindle in vivo measured by blocking polymerization with colchicine. J Cell Biol (1984) 2.42
Stability of microtubule attachment to metaphase kinetochores in PtK1 cells. J Cell Sci (1990) 2.34
Molecular architecture of the kinetochore-microtubule attachment site is conserved between point and regional centromeres. J Cell Biol (2008) 2.34
Pericentric chromatin is organized into an intramolecular loop in mitosis. Curr Biol (2008) 2.33
EB1 targets to kinetochores with attached, polymerizing microtubules. Mol Biol Cell (2002) 2.27
Poleward microtubule flux is a major component of spindle dynamics and anaphase a in mitotic Drosophila embryos. Curr Biol (2002) 2.27
Tension-dependent regulation of microtubule dynamics at kinetochores can explain metaphase congression in yeast. Mol Biol Cell (2005) 2.27
Kinetochore motility after severing between sister centromeres using laser microsurgery: evidence that kinetochore directional instability and position is regulated by tension. J Cell Sci (1995) 2.25
Diffusion coefficient of fluorescein-labeled tubulin in the cytoplasm of embryonic cells of a sea urchin: video image analysis of fluorescence redistribution after photobleaching. J Cell Biol (1984) 2.23
Spindle microtubules: thermodynamics of in vivo assembly and role in chromosome movement. Ann N Y Acad Sci (1975) 2.21
Roles of polymerization dynamics, opposed motors, and a tensile element in governing the length of Xenopus extract meiotic spindles. Mol Biol Cell (2005) 2.20
Functional organization of mitotic microtubules. Physical chemistry of the in vivo equilibrium system. Biophys J (1975) 2.18
The role of pre- and post-anaphase microtubules in the cytokinesis phase of the cell cycle. Curr Biol (2000) 2.17
Control of microtubule dynamics by Stu2p is essential for spindle orientation and metaphase chromosome alignment in yeast. Mol Biol Cell (2001) 2.16
Point centromeres contain more than a single centromere-specific Cse4 (CENP-A) nucleosome. J Cell Biol (2011) 2.14
Esperanto for histones: CENP-A, not CenH3, is the centromeric histone H3 variant. Chromosome Res (2013) 2.12
Anaphase spindle mechanics prevent mis-segregation of merotelically oriented chromosomes. Curr Biol (2004) 2.12
Anaphase A chromosome movement and poleward spindle microtubule flux occur At similar rates in Xenopus extract spindles. J Cell Biol (1998) 2.08
Structural and functional domains of the Drosophila ncd microtubule motor protein. J Biol Chem (1993) 2.06
How microtubules get fluorescent speckles. Biophys J (1998) 2.05
Welcome to a new kind of tension: translating kinetochore mechanics into a wait-anaphase signal. J Cell Sci (2010) 2.05
Assembly properties of fluorescein-labeled tubulin in vitro before and after fluorescence bleaching. J Cell Biol (1984) 2.03
Dynamics of microtubule depolymerization in monocytes. J Cell Biol (1986) 2.01
Poleward force at the kinetochore in metaphase depends on the number of kinetochore microtubules. J Cell Biol (1990) 2.00
Calcium-labile mitotic spindles isolated from sea urchin eggs (Lytechinus variegatus). J Cell Biol (1980) 1.97
Microtubule dynamics in the chromosomal spindle fiber: analysis by fluorescence and high-resolution polarization microscopy. Cell Motil Cytoskeleton (1988) 1.96
Pressure-induced depolymerization of spindle microtubules. I. Changes in birefringence and spindle length. J Cell Biol (1975) 1.95
Mechanisms of microtubule-based kinetochore positioning in the yeast metaphase spindle. Biophys J (2003) 1.93
Spinning disk confocal microscope system for rapid high-resolution, multimode, fluorescence speckle microscopy and green fluorescent protein imaging in living cells. Methods Enzymol (2003) 1.90
Mutants of the Drosophila ncd microtubule motor protein cause centrosomal and spindle pole defects in mitosis. J Cell Sci (1994) 1.87
The structure of microtubule ends during the elongation and shortening phases of dynamic instability examined by negative-stain electron microscopy. J Cell Sci (1990) 1.86
Microtubule assembly and kinetochore directional instability in vertebrate monopolar spindles: implications for the mechanism of chromosome congression. J Cell Sci (1994) 1.86
Isolation of microtubules and a dynein-like MgATPase from unfertilized sea urchin eggs. J Biol Chem (1984) 1.84
Recovery, visualization, and analysis of actin and tubulin polymer flow in live cells: a fluorescent speckle microscopy study. Biophys J (2003) 1.80
Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension. Mol Biol Cell (2002) 1.72
E-MAP-115 (ensconsin) associates dynamically with microtubules in vivo and is not a physiological modulator of microtubule dynamics. J Cell Sci (1999) 1.69
Glycophorin as a possible receptor for Plasmodium falciparum. Lancet (1982) 1.69
Dynein-like Mg2+-ATPase in mitotic spindles isolated from sea urchin embryos (Strongylocentrotus droebachiensis). J Cell Biol (1980) 1.67
Microtubules remodel actomyosin networks in Xenopus egg extracts via two mechanisms of F-actin transport. J Cell Biol (2000) 1.67
Pressure-induced depolymerization of spindle microtubules. III. Differential stability in HeLa cells. J Cell Biol (1976) 1.65
Dynamic positioning of mitotic spindles in yeast: role of microtubule motors and cortical determinants. Mol Biol Cell (2000) 1.65
Imaging green fluorescent protein fusion proteins in Saccharomyces cerevisiae. Curr Biol (1997) 1.63
Microtubule dynamics from mating through the first zygotic division in the budding yeast Saccharomyces cerevisiae. J Cell Biol (1999) 1.62
Identification of a mid-anaphase checkpoint in budding yeast. J Cell Biol (1997) 1.61
Checkpoint signals in grasshopper meiosis are sensitive to microtubule attachment, but tension is still essential. J Cell Sci (2001) 1.61
Asymmetric behavior of severed microtubule ends after ultraviolet-microbeam irradiation of individual microtubules in vitro. J Cell Biol (1989) 1.59
Feedback interactions between cell-cell adherens junctions and cytoskeletal dynamics in newt lung epithelial cells. Mol Biol Cell (2000) 1.58
Functional implications of cold-stable microtubules in kinetochore fibers of insect spermatocytes during anaphase. J Cell Biol (1980) 1.56
How tubulin subunits are lost from the shortening ends of microtubules. J Struct Biol (1997) 1.56