Protocols for experimentation with Schizosaccharomyces pombe.

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Published in Methods on July 01, 2004

Authors

Kathleen L Gould

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Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA (2002) 1.85

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Split decisions: coordinating cytokinesis in yeast. Trends Cell Biol (2005) 1.63

The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem (2008) 1.61

Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr Biol (2002) 1.57

Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell (2007) 1.53

Fission yeast Clp1p phosphatase affects G2/M transition and mitotic exit through Cdc25p inactivation. EMBO J (2004) 1.45

An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation. J Cell Biol (2003) 1.43

The GIN4 family kinase, Cdr2p, acts independently of septins in fission yeast. J Cell Sci (2004) 1.43

Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol Cell Biol (2005) 1.42

The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases. Biochemistry (2006) 1.38

The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1. J Cell Biol (2008) 1.38

The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring. J Cell Biol (2009) 1.37

Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol (2002) 1.36

The spindle pole body protein Cdc11p links Sid4p to the fission yeast septation initiation network. Mol Biol Cell (2002) 1.34

Mapping and analysis of phosphorylation sites: a quick guide for cell biologists. Mol Biol Cell (2013) 1.33

Phospho-regulation of the Cdc14/Clp1 phosphatase delays late mitotic events in S. pombe. Dev Cell (2006) 1.31

The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1. Curr Biol (2008) 1.29

Structural characterization of the fission yeast U5.U2/U6 spliceosome complex. Proc Natl Acad Sci U S A (2007) 1.28

Requirements of fission yeast septins for complex formation, localization, and function. Mol Biol Cell (2004) 1.27

Dma1 prevents mitotic exit and cytokinesis by inhibiting the septation initiation network (SIN). Dev Cell (2002) 1.23

Stepping into the ring: the SIN takes on contractile ring assembly. Genes Dev (2008) 1.22

Sid4p-Cdc11p assembles the septation initiation network and its regulators at the S. pombe SPB. Curr Biol (2004) 1.18

Setting the F-BAR: functions and regulation of the F-BAR protein family. Cell Cycle (2010) 1.18

Schizosaccharomyces pombe Git7p, a member of the Saccharomyces cerevisiae Sgtlp family, is required for glucose and cyclic AMP signaling, cell wall integrity, and septation. Eukaryot Cell (2002) 1.10

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A global census of fission yeast deubiquitinating enzyme localization and interaction networks reveals distinct compartmentalization profiles and overlapping functions in endocytosis and polarity. PLoS Biol (2010) 1.08

Role of septins and the exocyst complex in the function of hydrolytic enzymes responsible for fission yeast cell separation. Mol Biol Cell (2005) 1.05

Temporal control of contractile ring assembly by Plo1 regulation of myosin II recruitment by Mid1/anillin. Curr Biol (2011) 1.04

Cell wall remodeling at the fission yeast cell division site requires the Rho-GEF Rgf3p. J Cell Sci (2005) 1.03

State of the APC/C: organization, function, and structure. Crit Rev Biochem Mol Biol (2011) 1.02

Role of Hcn1 and its phosphorylation in fission yeast anaphase-promoting complex/cyclosome function. J Biol Chem (2006) 0.98

SIN-inhibitory phosphatase complex promotes Cdc11p dephosphorylation and propagates SIN asymmetry in fission yeast. Curr Biol (2011) 0.96

Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. PLoS One (2011) 0.96

The fission yeast septation initiation network (SIN) kinase, Sid2, is required for SIN asymmetry and regulates the SIN scaffold, Cdc11. Mol Biol Cell (2012) 0.95

Dma1 ubiquitinates the SIN scaffold, Sid4, to impede the mitotic localization of Plo1 kinase. EMBO J (2010) 0.94

Fully hydrated yeast cells imaged with electron microscopy. Biophys J (2011) 0.93

Mto2p, a novel fission yeast protein required for cytoplasmic microtubule organization and anchoring of the cytokinetic actin ring. Mol Biol Cell (2005) 0.93

A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission yeast borealin-like protein. Mol Biol Cell (2009) 0.93

Dim1p is required for efficient splicing and export of mRNA encoding lid1p, a component of the fission yeast anaphase-promoting complex. Eukaryot Cell (2005) 0.93

The Cdc14B phosphatase contributes to ciliogenesis in zebrafish. Development (2011) 0.91

Ppc89 links multiple proteins, including the septation initiation network, to the core of the fission yeast spindle-pole body. Mol Biol Cell (2006) 0.91

Identification of SIN pathway targets reveals mechanisms of crosstalk between NDR kinase pathways. Curr Biol (2013) 0.89

A highly efficient multifunctional tandem affinity purification approach applicable to diverse organisms. Mol Cell Proteomics (2012) 0.89

The role of Cdc14 phosphatases in the control of cell division. Biochem Soc Trans (2008) 0.89

Multisite phosphoregulation of Cdc25 activity refines the mitotic entrance and exit switches. Proc Natl Acad Sci U S A (2012) 0.89

Ace2p contributes to fission yeast septin ring assembly by regulating mid2+ expression. J Cell Sci (2005) 0.88

The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Structure (2010) 0.88

CK1 is required for a mitotic checkpoint that delays cytokinesis. Curr Biol (2013) 0.86

Identification of functional domains within the septation initiation network kinase, Cdc7. J Biol Chem (2006) 0.86

Comprehensive proteomics analysis reveals new substrates and regulators of the fission yeast clp1/cdc14 phosphatase. Mol Cell Proteomics (2013) 0.86

Regulation of cell cycle-specific gene expression in fission yeast by the Cdc14p-like phosphatase Clp1p. J Cell Sci (2010) 0.86

The kinesin-14 Klp2 is negatively regulated by the SIN for proper spindle elongation and telophase nuclear positioning. Mol Biol Cell (2012) 0.85

Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation. J Cell Biol (2011) 0.84

KSR1 is a functional protein kinase capable of serine autophosphorylation and direct phosphorylation of MEK1. Exp Cell Res (2010) 0.84

Dynamics of SIN asymmetry establishment. PLoS Comput Biol (2013) 0.83

On the cutting edge: post-translational modifications in cytokinesis. Trends Cell Biol (2011) 0.83

Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, and mutational analysis. Biochemistry (2003) 0.83

SIN-dependent phosphoinhibition of formin multimerization controls fission yeast cytokinesis. Genes Dev (2013) 0.83

Cytokinesis-based constraints on polarized cell growth in fission yeast. PLoS Genet (2012) 0.83

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Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress. Mol Biol Cell (2012) 0.79

Formin-based control of the actin cytoskeleton during cytokinesis. Biochem Soc Trans (2013) 0.79

Fission yeast Dma1 requires RING domain dimerization for its ubiquitin ligase activity and mitotic checkpoint function. J Biol Chem (2012) 0.79

Functional redundancy between Cdc14 phosphatases in zebrafish ciliogenesis. Dev Dyn (2012) 0.79

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An efficient fluorescent protein-based multifunctional affinity purification approach in mammalian cells. Methods Mol Biol (2014) 0.75

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