Published in Proc Natl Acad Sci U S A on February 20, 2007
SPIDER image processing for single-particle reconstruction of biological macromolecules from electron micrographs. Nat Protoc (2008) 4.60
Spliceosome structure and function. Cold Spring Harb Perspect Biol (2011) 4.01
Visualizing the splicing of single pre-mRNA molecules in whole cell extract. RNA (2007) 1.29
Cryo-EM structure of the yeast U4/U6.U5 tri-snRNP at 3.7 Å resolution. Nature (2016) 1.17
A protein-based EM label for RNA identifies the location of exons in spliceosomes. Nat Struct Mol Biol (2008) 1.04
LaSSO, a strategy for genome-wide mapping of intronic lariats and branch points using RNA-seq. Genome Res (2014) 1.02
The spliceosome: disorder and dynamics defined. Curr Opin Struct Biol (2014) 0.96
Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. PLoS One (2011) 0.96
Specific splicing defects in S. pombe carrying a degron allele of the Survival of Motor Neuron gene. EMBO J (2010) 0.93
Endogenous U2·U5·U6 snRNA complexes in S. pombe are intron lariat spliceosomes. RNA (2014) 0.86
Rearrangements within human spliceosomes captured after exon ligation. RNA (2013) 0.86
Structural and functional characterization of the N terminus of Schizosaccharomyces pombe Cwf10. Eukaryot Cell (2013) 0.84
Spliceosomal immunophilins. FEBS Lett (2008) 0.84
Detailed close-ups and the big picture of spliceosomes. Curr Opin Struct Biol (2008) 0.83
The use of trehalose in the preparation of specimens for molecular electron microscopy. Micron (2011) 0.81
CryoEM structures of two spliceosomal complexes: starter and dessert at the spliceosome feast. Curr Opin Struct Biol (2016) 0.80
The N-terminus of Prp1 (Prp6/U5-102 K) is essential for spliceosome activation in vivo. Nucleic Acids Res (2009) 0.79
RNA modularity for synthetic biology. F1000Prime Rep (2013) 0.79
Tls1 regulates splicing of shelterin components to control telomeric heterochromatin assembly and telomere length. Nucleic Acids Res (2014) 0.78
Splicing functions and global dependency on fission yeast slu7 reveal diversity in spliceosome assembly. Mol Cell Biol (2013) 0.77
Structural and mechanistic insights into human splicing factor SF3b complex derived using an integrated approach guided by the cryo-EM density maps. RNA Biol (2016) 0.76
Structure of spliceosomal ribonucleoproteins. F1000 Biol Rep (2010) 0.76
A unique spatial arrangement of the snRNPs within the native spliceosome emerges from in silico studies. Structure (2012) 0.76
While the revolution will not be crystallized, biochemistry reigns supreme. Protein Sci (2016) 0.75
FgPrp4 Kinase Is Important for Spliceosome B-Complex Activation and Splicing Efficiency in Fusarium graminearum. PLoS Genet (2016) 0.75
Human DBR1 modulates the recycling of snRNPs to affect alternative RNA splicing and contributes to the suppression of cancer development. Oncogene (2017) 0.75
Cryo-electron microscopy snapshots of the spliceosome: structural insights into a dynamic ribonucleoprotein machine. Nat Struct Mol Biol (2017) 0.75
UCSF Chimera--a visualization system for exploratory research and analysis. J Comput Chem (2004) 112.47
A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol (1999) 25.13
SPIDER and WEB: processing and visualization of images in 3D electron microscopy and related fields. J Struct Biol (1996) 22.89
Accurate determination of local defocus and specimen tilt in electron microscopy. J Struct Biol (2003) 14.19
A new generation of the IMAGIC image processing system. J Struct Biol (1996) 12.57
Optimal determination of particle orientation, absolute hand, and contrast loss in single-particle electron cryomicroscopy. J Mol Biol (2003) 12.04
Pre-mRNA splicing: awash in a sea of proteins. Mol Cell (2003) 8.28
MRC image processing programs. J Struct Biol (1996) 6.91
Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin. Nature (1994) 6.54
Negative Staining and Image Classification - Powerful Tools in Modern Electron Microscopy. Biol Proced Online (2004) 6.14
Three-dimensional reconstruction from a single-exposure, random conical tilt series applied to the 50S ribosomal subunit of Escherichia coli. J Microsc (1987) 5.18
Crystal structures of two Sm protein complexes and their implications for the assembly of the spliceosomal snRNPs. Cell (1999) 4.57
Angular reconstitution: a posteriori assignment of projection directions for 3D reconstruction. Ultramicroscopy (1987) 4.43
Crystal structure of the spliceosomal 15.5kD protein bound to a U4 snRNA fragment. Mol Cell (2000) 4.42
A doughnut-shaped heteromer of human Sm-like proteins binds to the 3'-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro. EMBO J (1999) 3.80
Small nuclear ribonucleoprotein remodeling during catalytic activation of the spliceosome. Science (2002) 3.59
Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. RNA (2002) 3.57
Crystal structure of the spliceosomal U2B"-U2A' protein complex bound to a fragment of U2 small nuclear RNA. Nature (1998) 3.08
The Prp19p-associated complex in spliceosome activation. Science (2003) 3.06
Noise bias in the refinement of structures derived from single particles. Ultramicroscopy (2004) 3.00
Cotranscriptional spliceosome assembly occurs in a stepwise fashion and requires the cap binding complex. Mol Cell (2005) 2.68
Molecular architecture of the multiprotein splicing factor SF3b. Science (2003) 2.63
Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol (2002) 2.32
RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex. EMBO J (2001) 2.28
Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe. Yeast (2001) 2.23
U2AF homolog required for splicing in vivo. Science (1993) 2.08
The crystal structure of a heptameric archaeal Sm protein: Implications for the eukaryotic snRNP core. Proc Natl Acad Sci U S A (2001) 1.94
Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing. Mol Cell Biol (1999) 1.92
Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA (2002) 1.85
Precise branch point mapping and quantification of splicing intermediates. Nucleic Acids Res (1997) 1.75
Crystal structure of a heptameric Sm-like protein complex from archaea: implications for the structure and evolution of snRNPs. J Mol Biol (2001) 1.71
Crystal structures of the Pyrococcus abyssi Sm core and its complex with RNA. Common features of RNA binding in archaea and eukarya. J Biol Chem (2002) 1.52
Three-dimensional structure of C complex spliceosomes by electron microscopy. Nat Struct Mol Biol (2004) 1.51
Three-dimensional reconstruction from random projections: orientational alignment via Radon transforms. Ultramicroscopy (1994) 1.42
Single particle reconstructions of the transferrin-transferrin receptor complex obtained with different specimen preparation techniques. J Mol Biol (2005) 1.38
Organization of core spliceosomal components U5 snRNA loop I and U4/U6 Di-snRNP within U4/U6.U5 Tri-snRNP as revealed by electron cryomicroscopy. Mol Cell (2006) 1.33
Three-dimensional structure of the native spliceosome by cryo-electron microscopy. Mol Cell (2004) 1.29
Pre-spliceosome formation in S.pombe requires a stable complex of SF1-U2AF(59)-U2AF(23). EMBO J (2002) 1.27
Three-dimensional structure of a pre-catalytic human spliceosomal complex B. Nat Struct Mol Biol (2004) 1.25
The structure and biochemical properties of the human spliceosomal protein U1C. J Mol Biol (2004) 1.23
Crystal structure of a core spliceosomal protein interface. Proc Natl Acad Sci U S A (2006) 1.20
Arrested yeast splicing complexes indicate stepwise snRNP recruitment during in vivo spliceosome assembly. RNA (2006) 1.15
Identification, characterization and crystal structure analysis of the human spliceosomal U5 snRNP-specific 15 kD protein. J Mol Biol (1999) 1.14
Biochemical and NMR analyses of an SF3b155-p14-U2AF-RNA interaction network involved in branch point definition during pre-mRNA splicing. RNA (2006) 1.09
The SF3b155 N-terminal domain is a scaffold important for splicing. Biochemistry (2006) 0.91
Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin. J Biol Chem (2000) 0.89
A virulence locus of Pseudomonas aeruginosa encodes a protein secretion apparatus. Science (2006) 7.84
Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Cell (2002) 7.03
STIM1 clusters and activates CRAC channels via direct binding of a cytosolic domain to Orai1. Cell (2009) 6.92
Negative Staining and Image Classification - Powerful Tools in Modern Electron Microscopy. Biol Proced Online (2004) 6.14
Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature (2005) 5.44
Neurodegenerative disease: amyloid pores from pathogenic mutations. Nature (2002) 5.32
Multiple associated proteins regulate proteasome structure and function. Mol Cell (2002) 4.97
Molecular model for a complete clathrin lattice from electron cryomicroscopy. Nature (2004) 4.48
The RIP1/RIP3 necrosome forms a functional amyloid signaling complex required for programmed necrosis. Cell (2012) 4.29
Shotgun identification of protein modifications from protein complexes and lens tissue. Proc Natl Acad Sci U S A (2002) 3.70
Structure of the human transferrin receptor-transferrin complex. Cell (2004) 3.64
Latent TGF-β structure and activation. Nature (2011) 3.42
Structure of the human mTOR complex I and its implications for rapamycin inhibition. Mol Cell (2010) 3.33
Activation of leukocyte beta2 integrins by conversion from bent to extended conformations. Immunity (2006) 3.11
Structure of integrin alpha5beta1 in complex with fibronectin. EMBO J (2003) 2.99
Aquaporin-0 membrane junctions reveal the structure of a closed water pore. Nature (2004) 2.94
Alpha-synuclein, especially the Parkinson's disease-associated mutants, forms pore-like annular and tubular protofibrils. J Mol Biol (2002) 2.92
ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins. Mol Cell (2005) 2.82
Implications of the aquaporin-4 structure on array formation and cell adhesion. J Mol Biol (2005) 2.50
Quaternary structure, protein dynamics, and synaptic function of SAP97 controlled by L27 domain interactions. Neuron (2004) 2.49
Death domain assembly mechanism revealed by crystal structure of the oligomeric PIDDosome core complex. Cell (2007) 2.48
Assembly of the SIR complex and its regulation by O-acetyl-ADP-ribose, a product of NAD-dependent histone deacetylation. Cell (2005) 2.47
Structural basis for dsRNA recognition, filament formation, and antiviral signal activation by MDA5. Cell (2012) 2.34
Proteomics analysis reveals stable multiprotein complexes in both fission and budding yeasts containing Myb-related Cdc5p/Cef1p, novel pre-mRNA splicing factors, and snRNAs. Mol Cell Biol (2002) 2.32
Structural insights into the U-box, a domain associated with multi-ubiquitination. Nat Struct Biol (2003) 2.26
A structure-based mechanism for vesicle capture by the multisubunit tethering complex Dsl1. Cell (2009) 2.24
Structural basis for allosteric regulation of human ribonucleotide reductase by nucleotide-induced oligomerization. Nat Struct Mol Biol (2011) 2.23
Structure and different conformational states of native AMPA receptor complexes. Nature (2005) 2.22
Cryo-electron tomography of clathrin-coated vesicles: structural implications for coat assembly. J Mol Biol (2006) 2.17
Signaling conformations of the tall cytokine receptor gp130 when in complex with IL-6 and IL-6 receptor. Nat Struct Mol Biol (2005) 2.12
Structure of the influenza virus A H5N1 nucleoprotein: implications for RNA binding, oligomerization, and vaccine design. FASEB J (2008) 2.10
The architecture of the multisubunit TRAPP I complex suggests a model for vesicle tethering. Cell (2006) 2.10
The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations. Nat Struct Mol Biol (2010) 2.08
Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site. Mol Cell (2010) 2.02
Structural basis of Dscam isoform specificity. Nature (2007) 2.02
Cooperative assembly and dynamic disassembly of MDA5 filaments for viral dsRNA recognition. Proc Natl Acad Sci U S A (2011) 1.99
The structure of aquaporins. Q Rev Biophys (2006) 1.90
Role of the conserved Sir3-BAH domain in nucleosome binding and silent chromatin assembly. Mol Cell (2007) 1.89
Tandem affinity purification and identification of protein complex components. Methods (2004) 1.87
Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. RNA (2002) 1.85
Mixtures of wild-type and a pathogenic (E22G) form of Abeta40 in vitro accumulate protofibrils, including amyloid pores. J Mol Biol (2003) 1.81
Structure of an auxilin-bound clathrin coat and its implications for the mechanism of uncoating. Nature (2004) 1.81
The HEAT repeat protein Blm10 regulates the yeast proteasome by capping the core particle. Nat Struct Mol Biol (2005) 1.78
The hexamer structure of Rift Valley fever virus nucleoprotein suggests a mechanism for its assembly into ribonucleoprotein complexes. PLoS Pathog (2011) 1.78
Identification and characterization of two novel proteins affecting fission yeast gamma-tubulin complex function. Mol Biol Cell (2004) 1.75
The PCH family protein, Cdc15p, recruits two F-actin nucleation pathways to coordinate cytokinetic actin ring formation in Schizosaccharomyces pombe. J Cell Biol (2003) 1.71
Three-dimensional reconstruction of the Shigella T3SS transmembrane regions reveals 12-fold symmetry and novel features throughout. Nat Struct Mol Biol (2009) 1.70
Properties of the hybrid form of the 26S proteasome containing both 19S and PA28 complexes. EMBO J (2002) 1.68
Architecture of Ure2p prion filaments: the N-terminal domains form a central core fiber. J Biol Chem (2003) 1.66
Structural snapshots of full-length Jak1, a transmembrane gp130/IL-6/IL-6Rα cytokine receptor complex, and the receptor-Jak1 holocomplex. Structure (2011) 1.63
Aquaporin-0 membrane junctions form upon proteolytic cleavage. J Mol Biol (2004) 1.63
Split decisions: coordinating cytokinesis in yeast. Trends Cell Biol (2005) 1.63
The spindle checkpoint functions of Mad3 and Mad2 depend on a Mad3 KEN box-mediated interaction with Cdc20-anaphase-promoting complex (APC/C). J Biol Chem (2008) 1.61
Allosteric beta1 integrin antibodies that stabilize the low affinity state by preventing the swing-out of the hybrid domain. J Biol Chem (2004) 1.60
Structural transitions of complement component C3 and its activation products. Proc Natl Acad Sci U S A (2006) 1.57
Proteomics analysis identifies new components of the fission and budding yeast anaphase-promoting complexes. Curr Biol (2002) 1.57
RIG-I forms signaling-competent filaments in an ATP-dependent, ubiquitin-independent manner. Mol Cell (2013) 1.57
Structural and functional characterization of the integral membrane protein VDAC-1 in lipid bilayer nanodiscs. J Am Chem Soc (2009) 1.55
Structural organization of the anaphase-promoting complex bound to the mitotic activator Slp1. Mol Cell (2007) 1.53
The monopolin complex crosslinks kinetochore components to regulate chromosome-microtubule attachments. Cell (2010) 1.48
Functional and structural stability of the epidermal growth factor receptor in detergent micelles and phospholipid nanodiscs. Biochemistry (2008) 1.48
Structure of a VEGF-VEGF receptor complex determined by electron microscopy. Nat Struct Mol Biol (2007) 1.48
Molecular basis for insulin fibril assembly. Proc Natl Acad Sci U S A (2009) 1.47
Fission yeast Clp1p phosphatase affects G2/M transition and mitotic exit through Cdc25p inactivation. EMBO J (2004) 1.45
Simultaneous visualization of the extracellular and cytoplasmic domains of the epidermal growth factor receptor. Nat Struct Mol Biol (2011) 1.45
An anillin homologue, Mid2p, acts during fission yeast cytokinesis to organize the septin ring and promote cell separation. J Cell Biol (2003) 1.43
The GIN4 family kinase, Cdr2p, acts independently of septins in fission yeast. J Cell Sci (2004) 1.43
An intersubunit disulfide bond prevents in vitro aggregation of a superoxide dismutase-1 mutant linked to familial amytrophic lateral sclerosis. Biochemistry (2004) 1.43
Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Mol Cell Biol (2005) 1.42
Molecular electron microscopy: state of the art and current challenges. ACS Chem Biol (2008) 1.41
Structure of hepatitis E virion-sized particle reveals an RNA-dependent viral assembly pathway. J Biol Chem (2010) 1.40
The Prp19 U-box crystal structure suggests a common dimeric architecture for a class of oligomeric E3 ubiquitin ligases. Biochemistry (2006) 1.38
The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1. J Cell Biol (2008) 1.38
Principles of membrane protein interactions with annular lipids deduced from aquaporin-0 2D crystals. EMBO J (2010) 1.38
Single particle reconstructions of the transferrin-transferrin receptor complex obtained with different specimen preparation techniques. J Mol Biol (2005) 1.38
The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring. J Cell Biol (2009) 1.37
Mass analysis by scanning transmission electron microscopy and electron diffraction validate predictions of stacked beta-solenoid model of HET-s prion fibrils. J Biol Chem (2006) 1.36
Removal of a single alpha-tubulin gene intron suppresses cell cycle arrest phenotypes of splicing factor mutations in Saccharomyces cerevisiae. Mol Cell Biol (2002) 1.36
Molecular architecture of the vesicular stomatitis virus RNA polymerase. Proc Natl Acad Sci U S A (2010) 1.35
The spindle pole body protein Cdc11p links Sid4p to the fission yeast septation initiation network. Mol Biol Cell (2002) 1.34
Monolayer purification: a rapid method for isolating protein complexes for single-particle electron microscopy. Proc Natl Acad Sci U S A (2008) 1.34
A pH-regulated dimeric bouquet in the structure of von Willebrand factor. EMBO J (2011) 1.34
Kinetic mechanism for viral dsRNA length discrimination by MDA5 filaments. Proc Natl Acad Sci U S A (2012) 1.33
Mapping and analysis of phosphorylation sites: a quick guide for cell biologists. Mol Biol Cell (2013) 1.33
Production and characterization of astrocyte-derived human apolipoprotein E isoforms from immortalized astrocytes and their interactions with amyloid-beta. Neurobiol Dis (2005) 1.32
Requirement of open headpiece conformation for activation of leukocyte integrin alphaXbeta2. Proc Natl Acad Sci U S A (2010) 1.32
Huntingtin facilitates polycomb repressive complex 2. Hum Mol Genet (2009) 1.32
Phospho-regulation of the Cdc14/Clp1 phosphatase delays late mitotic events in S. pombe. Dev Cell (2006) 1.31
The SIN kinase Sid2 regulates cytoplasmic retention of the S. pombe Cdc14-like phosphatase Clp1. Curr Biol (2008) 1.29
Structural insight into pre-B cell receptor function. Science (2007) 1.29
Requirements of fission yeast septins for complex formation, localization, and function. Mol Biol Cell (2004) 1.27
Oligomeric states of bacteriophage T7 gene 4 primase/helicase. J Mol Biol (2006) 1.26