Nonnatural amino acid incorporation into the methionine 214 position of the metzincin Pseudomonas aeruginosa alkaline protease.

PubWeight™: 0.79‹?›

🔗 View Article (PMC 1266349)

Published in BMC Biochem on October 12, 2005

Authors

Paula Walasek1, John F Honek

Author Affiliations

1: Department of Chemistry, University of Waterloo, 200 University Avenue, Waterloo, Ontario, Canada N2L 3G1. pwalasek@scimail.uwaterloo.ca

Articles cited by this

Matrix metalloproteinases. J Biol Chem (1999) 12.08

Association of the ADAM33 gene with asthma and bronchial hyperresponsiveness. Nature (2002) 8.28

Tissue inhibitors of metalloproteinases: evolution, structure and function. Biochim Biophys Acta (2000) 5.39

Matrix metalloproteinases: they're not just for matrix anymore! Curr Opin Cell Biol (2001) 4.99

Recent Advances in Zinc Enzymology. Chem Rev (1996) 4.28

Families of zinc metalloproteases. FEBS Lett (1994) 3.40

Suggestions for "safe" residue substitutions in site-directed mutagenesis. J Mol Biol (1991) 2.80

Three-dimensional structure of the alkaline protease of Pseudomonas aeruginosa: a two-domain protein with a calcium binding parallel beta roll motif. EMBO J (1993) 2.80

Astacins, serralysins, snake venom and matrix metalloproteinases exhibit identical zinc-binding environments (HEXXHXXGXXH and Met-turn) and topologies and should be grouped into a common family, the 'metzincins'. FEBS Lett (1993) 2.72

The metzincins--topological and sequential relations between the astacins, adamalysins, serralysins, and matrixins (collagenases) define a superfamily of zinc-peptidases. Protein Sci (1995) 2.63

New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol Bioeng (1999) 2.04

Structural aspects of the metzincin clan of metalloendopeptidases. Mol Biotechnol (2003) 1.87

Sequence of a cluster of genes controlling synthesis and secretion of alkaline protease in Pseudomonas aeruginosa: relationships to other secretory pathways. Gene (1992) 1.81

Epidemic population structure of Pseudomonas aeruginosa: evidence for a clone that is pathogenic to the eye and that has a distinct combination of virulence factors. Infect Immun (2001) 1.74

Site-specific incorporation of biophysical probes into proteins. Proc Natl Acad Sci U S A (1994) 1.66

Incorporation of nonnatural amino acids into proteins. Annu Rev Biochem (2004) 1.64

Penetration, adhesion, and fusion in mammalian sperm-egg interaction. Science (2002) 1.53

Shedding light on sheddases: role in growth and development. Bioessays (2002) 1.47

Practical considerations in refolding proteins from inclusion bodies. Protein Expr Purif (2003) 1.45

Mechanisms involved in the evasion of the host defence by Pseudomonas aeruginosa. Immunol Lett (1991) 1.40

Construction and deconstruction of bacterial inclusion bodies. J Biotechnol (2002) 1.31

Molecular chaperones, folding catalysts, and the recovery of active recombinant proteins from E. coli. To fold or to refold. Appl Biochem Biotechnol (1997) 1.30

Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455. Infect Immun (1990) 1.24

Open conformation of a substrate-binding cleft: 19F NMR studies of cleft angle in the D-galactose chemosensory receptor. Biochemistry (1991) 1.23

Structural features of a superfamily of zinc-endopeptidases: the metzincins. Curr Opin Struct Biol (1995) 1.22

Role of bacterial proteases in pseudomonal and serratial keratitis. Biol Chem (2004) 1.21

Cloning of the Pseudomonas aeruginosa alkaline protease gene and secretion of the protease into the medium by Escherichia coli. J Bacteriol (1990) 1.15

Context-dependent protein stabilization by methionine-to-leucine substitution shown in T4 lysozyme. Protein Sci (1998) 1.14

The preparation of 19F-labeled proteins for NMR studies. Methods Enzymol (2004) 1.13

Insights into the mechanism of Escherichia coli methionine aminopeptidase from the structural analysis of reaction products and phosphorus-based transition-state analogues. Biochemistry (1999) 1.10

Tolerance of point substitution of methionine for isoleucine in hen egg white lysozyme. Protein Eng (2001) 1.06

Interactions of non-detergent sulfobetaines with early folding intermediates facilitate in vitro protein renaturation. Eur J Biochem (1998) 1.04

Pseudomonas aeruginosa alkaline protease: evidence for secretion genes and study of secretion mechanism. J Bacteriol (1991) 1.04

Comparison of vertebrate collagenase and gelatinase using a new fluorogenic substrate peptide. J Biol Chem (1989) 1.04

The role of bacterial proteases in the pathogenesis of cystic fibrosis. Am J Respir Crit Care Med (1994) 1.04

The canonical methionine 392 of matrix metalloproteinase 2 (gelatinase A) is not required for catalytic efficiency or structural integrity: probing the role of the methionine-turn in the metzincin metalloprotease superfamily. J Biol Chem (2004) 0.99

Design, synthesis, and evaluation of a mechanism-based inhibitor for gelatinase A. J Org Chem (2005) 0.96

The recombinant catalytic domain of human neutrophil collagenase lacks type I collagen substrate specificity. Biochem Biophys Res Commun (1993) 0.92

Purification and characterization of the human stromelysin catalytic domain expressed in Escherichia coli. Biochemistry (1992) 0.92

Crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 and its conformational changes on ligand binding. J Biochem (1995) 0.87

Efforts towards the design of 'teflon' proteins: in vivo translation with trifluorinated leucine and methionine analogues. Chem Biodivers (2004) 0.86

The conserved methionine residue of the metzincins: a site-directed mutagenesis study. J Mol Biol (2001) 0.86

(19)F NMR studies of the leucine-isoleucine-valine binding protein: evidence that a closed conformation exists in solution. J Biomol Struct Dyn (2003) 0.86

Cloning and expression of the alkaline proteinase gene from Pseudomonas aeruginosa IFO 3455. J Bacteriol (1989) 0.85

Production of selenomethionine-labeled recombinant human neutrophil collagenase in Escherichia coli. J Biotechnol (1995) 0.84

Incorporation of fluorescently labeled nonnatural amino acids into proteins in an E. coli in vitro translation system. Nucleic Acids Res Suppl (2003) 0.82

cDNA cloning, bacterial expression, in vitro renaturation and affinity purification of the zinc endopeptidase astacin. Biochem J (1999) 0.82

Elucidation of solvent exposure, side-chain reactivity, and steric demands of the trifluoromethionine residue in a recombinant protein. Biochemistry (2001) 0.81

Kinetic characterization of the serralysins: a divergent catalytic mechanism pertaining to astacin-type metalloproteases. Biochemistry (1997) 0.79

Expression, purification, characterization, and X-ray analysis of selenomethionine 215 variant of leukocyte collagenase. J Protein Chem (1997) 0.79

Analysis of protease activity in human otitis media. Otolaryngol Head Neck Surg (1998) 0.79

Shedding growth factors in cardiac hypertrophy. Nat Med (2002) 0.79

Long-range effect of mutation of calcium binding aspartates [correction of asparates] on the catalytic activity of alkaline protease from Pseudomonas aeruginosa. J Biochem (1998) 0.78

Articles by these authors

Methionine in and out of proteins: targets for drug design. Curr Med Chem (2002) 1.38

Reduction potential tuning of the blue copper center in Pseudomonas aeruginosa azurin by the axial methionine as probed by unnatural amino acids. J Am Chem Soc (2006) 1.33

Biological chemistry of naturally occurring thiols of microbial and marine origin. J Nat Prod (2005) 1.18

Use of analogues of methionine and methionyl adenylate to sample conformational changes during catalysis in Escherichia coli methionyl-tRNA synthetase. J Mol Biol (2003) 1.11

Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis, Pseudomonas aeruginosa and Neisseria meningitidis enzymes. Biochem J (2004) 1.06

Phosphate transfer from inositol pyrophosphates InsP5PP and InsP4(PP)2: a semi-empirical investigation. Bioorg Med Chem Lett (2006) 1.05

Structure of the thiostrepton resistance methyltransferase.S-adenosyl-L-methionine complex and its interaction with ribosomal RNA. J Biol Chem (2009) 0.96

Enzyme-assisted suicide: molecular basis for the antifungal activity of 5-hydroxy-4-oxonorvaline by potent inhibition of homoserine dehydrogenase. Chem Biol (2003) 0.93

Structural variation in bacterial glyoxalase I enzymes: investigation of the metalloenzyme glyoxalase I from Clostridium acetobutylicum. J Biol Chem (2011) 0.93

Investigation of metal binding and activation of Escherichia coli glyoxalase I: kinetic, thermodynamic and mutagenesis studies. Biochem J (2004) 0.89

Pseudomonas aeruginosa contains multiple glyoxalase I-encoding genes from both metal activation classes. Biochim Biophys Acta (2007) 0.89

(19)F NMR studies of the leucine-isoleucine-valine binding protein: evidence that a closed conformation exists in solution. J Biomol Struct Dyn (2003) 0.86

Bacterial glyoxalase enzymes. Semin Cell Dev Biol (2011) 0.86

Semi-synthetic analogues of thiostrepton delimit the critical nature of tail region modifications in the control of protein biosynthesis and antibacterial activity. Bioorg Med Chem (2010) 0.86

Microbial glyoxalase enzymes: metalloenzymes controlling cellular levels of methylglyoxal. Drug Metabol Drug Interact (2008) 0.85

Escherichia coli glyoxalase II is a binuclear zinc-dependent metalloenzyme. Arch Biochem Biophys (2006) 0.84

Synthesis of an alpha-aminophosphonate nucleoside as an inhibitor of S-adenosyl-L-homocysteine hydrolase. Bioorg Med Chem Lett (2002) 0.83

Ab initio studies of the properties of intracellular thiols ergothioneine and ovothiol. Bioorg Med Chem Lett (2005) 0.81

Emerging bacterial enzyme targets. Curr Opin Investig Drugs (2007) 0.80

Synthesis and biological activity of novel S-adenosyl-L-homocysteine hydrolase inhibitors. Bioorg Med Chem (2003) 0.79

Conformational selectivity of peptides for single-walled carbon nanotubes. J Phys Chem B (2006) 0.78

15N-1H HSQC NMR evidence for distinct specificity of two active sites in Escherichia coli glyoxalase I. Biochemistry (2008) 0.78

Glyoxalase biochemistry. Biomol Concepts (2015) 0.78

Modulating glyoxalase I metal selectivity by deletional mutagenesis: underlying structural factors contributing to nickel activation profiles. Metallomics (2015) 0.77

Controllable delivery of small-molecule compounds to targeted cells utilizing carbon nanotubes. J Am Chem Soc (2011) 0.77

Molecular interactions between thiostrepton and the TipAS protein from Streptomyces lividans. Chembiochem (2014) 0.77

Mechanistic studies on the enzymatic processing of fluorinated methionine analogues by Trichomonas vaginalis methionine γ-lyase. Biochem J (2011) 0.77

Conformational analyses of mycothiol, a critical intracellular glycothiol in Mycobacteria. Carbohydr Res (2006) 0.77

Ni2+-activated glyoxalase I from Escherichia coli: substrate specificity, kinetic isotope effects and evolution within the βαβββ superfamily. J Inorg Biochem (2011) 0.76

Electronic structure calculations on the thiazole-containing antibiotic thiostrepton: molecular mechanics, semi-empirical and ab initio analyses. Bioorg Med Chem Lett (2005) 0.75

Current and future perspectives on the chemotherapy of the parasitic protozoa Trichomonas vaginalis and Entamoeba histolytica. Future Med Chem (2009) 0.75

The crystal structure of a homodimeric Pseudomonas glyoxalase I enzyme reveals asymmetric metallation commensurate with half-of-sites activity. Chemistry (2014) 0.75

Research spotlight: Bionanotechnology: small can have a big impact in the medical sciences: a WIN-win situation. Part 1. Future Med Chem (2010) 0.75

Bionanotechnology and bionanomaterials: John Honek explains the good things that can come in very small packages. BMC Biochem (2013) 0.75