Deletion by in vivo recombination shows that the 28-kilodalton cytolytic polypeptide from Bacillus thuringiensis subsp. israelensis is not essential for mosquitocidal activity.

PubWeight™: 1.94‹?› | Rank: Top 2%

🔗 View Article (PMC 207948)

Published in J Bacteriol on June 01, 1991

Authors

A Delécluse1, J F Charles, A Klier, G Rapoport

Author Affiliations

1: Unité de Biochimie Microbienne, URA 1300 CNRS, Paris, France.

Articles citing this

Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol Mol Biol Rev (1998) 13.47

Influence of Exposure to Single versus Multiple Toxins of Bacillus thuringiensis subsp. israelensis on Development of Resistance in the Mosquito Culex quinquefasciatus (Diptera: Culicidae). Appl Environ Microbiol (1997) 2.20

A 20-kilodalton protein preserves cell viability and promotes CytA crystal formation during sporulation in Bacillus thuringiensis. J Bacteriol (1993) 1.95

Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes. Microbiol Rev (1993) 1.88

CytA enables CryIV endotoxins of Bacillus thuringiensis to overcome high levels of CryIV resistance in the mosquito, Culex quinquefasciatus. Proc Natl Acad Sci U S A (1997) 1.84

High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes. Appl Environ Microbiol (1993) 1.83

Expression of cryIVA and cryIVB Genes, Independently or in Combination, in a Crystal-Negative Strain of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (1993) 1.79

Properties of a 72-kilodalton mosquitocidal protein from Bacillus thuringiensis subsp. morrisoni PG-14 expressed in B. thuringiensis subsp. kurstaki by using the shuttle vector pHT3101. Appl Environ Microbiol (1992) 1.59

Cloning and expression of a novel toxin gene from Bacillus thuringiensis subsp. jegathesan encoding a highly mosquitocidal protein. Appl Environ Microbiol (1995) 1.44

Characterization of two genes encoding Bacillus thuringiensis insecticidal crystal proteins toxic to Coleoptera species. Appl Environ Microbiol (1992) 1.40

PCR-based approach for detection of novel Bacillus thuringiensis cry genes. Appl Environ Microbiol (1997) 1.36

Characterization of the operon encoding the alternative sigma(B) factor from Bacillus anthracis and its role in virulence. J Bacteriol (2000) 1.23

Cloning and expression of the cryIVD gene of Bacillus thuringiensis subsp. israelensis in the cyanobacterium Agmenellum quadruplicatum PR-6 and its resulting larvicidal activity. Appl Environ Microbiol (1992) 1.18

Identification of a gene for Cyt1A-like hemolysin from Bacillus thuringiensis subsp. medellin and expression in a crystal-negative B. thuringiensis strain. Appl Environ Microbiol (1997) 1.18

Tn5401, a new class II transposable element from Bacillus thuringiensis. J Bacteriol (1994) 1.17

Cytolytic toxin Cyt1A and its mechanism of membrane damage: data and hypotheses. Appl Environ Microbiol (2003) 1.15

Improvement of Bacillus sphaericus toxicity against dipteran larvae by integration, via homologous recombination, of the Cry11A toxin gene from Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (1997) 1.13

Molecular characterization of two novel crystal protein genes from Bacillus thuringiensis subsp. thompsoni. J Bacteriol (1992) 1.12

Variable cross-resistance to Cry11B from Bacillus thuringiensis subsp. jegathesan in Culex quinquefasciatus (Diptera: Culicidae) resistant to single or multiple toxins of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (1998) 1.10

New strategy for identification of novel Cry-type genes from Bacillus thuringiensis strains. Appl Environ Microbiol (2005) 1.09

Cloning and expression of the first anaerobic toxin gene from Clostridium bifermentans subsp. malaysia, encoding a new mosquitocidal protein with homologies to Bacillus thuringiensis delta-endotoxins. J Bacteriol (1996) 1.04

Mosquito larvicidal activity of Escherichia coli with combinations of genes from Bacillus thuringiensis subsp. israelensis. J Bacteriol (1995) 1.00

Cloning and characterization of a cytolytic and mosquitocidal delta-endotoxin from Bacillus thuringiensis subsp. jegathesan. Appl Environ Microbiol (1997) 0.98

Role of the CryIVD polypeptide in the overall toxicity of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (1993) 0.95

Effect of promoter modification on mosquitocidal cryIVB gene expression in Synechococcus sp. strain PCC 7942. Appl Environ Microbiol (1993) 0.93

The Bacillus thuringiensis cyt genes for hemolytic endotoxins constitute a gene family. Appl Environ Microbiol (2001) 0.88

Production of Cry11A and Cry11Ba toxins in Bacillus sphaericus confers toxicity towards Aedes aegypti and resistant Culex populations. Appl Environ Microbiol (1999) 0.85

Conjugal transfer of a toxin-coding megaplasmid from Bacillus thuringiensis subsp. israelensis to mosquitocidal strains of Bacillus sphaericus. Appl Environ Microbiol (2006) 0.85

Enhanced production of insecticidal proteins in Bacillus thuringiensis strains carrying an additional crystal protein gene in their chromosomes. Appl Environ Microbiol (1995) 0.83

Mtx toxins from Lysinibacillus sphaericus enhance mosquitocidal cry-toxin activity and suppress cry-resistance in Culex quinquefasciatus. J Invertebr Pathol (2013) 0.80

A nonribosomal peptide synthetase gene tzw1 is involved in zwittermicin A biosynthesis in Bacillus thuringiensis G03. Curr Microbiol (2008) 0.79

Cloning and characterization of a novel crystal protein from a native Bacillus thuringiensis isolate highly active against Aedes aegypti. Curr Microbiol (2007) 0.75

Articles cited by this

Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol (1975) 503.08

A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res (1979) 214.95

Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev (1989) 16.67

Construction and properties of an integrable plasmid for Bacillus subtilis. J Bacteriol (1983) 7.12

Bacillus thuringiensis var israelensis crystal delta-endotoxin: effects on insect and mammalian cells in vitro and in vivo. J Cell Sci (1983) 4.85

Transformation and expression of a cloned delta-endotoxin gene in Bacillus thuringiensis. FEMS Microbiol Lett (1989) 3.83

Nucleotide sequence of the erythromycin resistance gene of the conjugative transposon Tn1545. Nucleic Acids Res (1990) 3.74

Gene amplification in Bacillus subtilis. J Gen Microbiol (1984) 2.87

Bacillus thuringiensis var. israelensis delta-endotoxin. Nucleotide sequence and characterization of the transcripts in Bacillus thuringiensis and Escherichia coli. J Mol Biol (1986) 2.62

Isolation of a relatively nontoxic 65-kilodalton protein inclusion from the parasporal body of Bacillus thuringiensis subsp. israelensis. J Bacteriol (1986) 2.45

Comparative toxicity of Bacillus thuringiensis var. israelensis crystal proteins in vivo and in vitro. J Gen Microbiol (1988) 2.30

A large transmissible plasmid is required for crystal toxin production in Bacillus thuringiensis variety israelensis. Plasmid (1984) 2.30

Stable gene amplification in the chromosome of Bacillus subtilis. Gene (1985) 2.25

Delta endotoxin of Bacillus thuringiensis subsp. israelensis. J Bacteriol (1985) 2.07

Generalized transduction in Bacillus thuringiensis var. berliner 1715 using bacteriophage CP-54Ber. J Gen Microbiol (1980) 2.07

Characterization of the genes encoding the haemolytic toxin and the mosquitocidal delta-endotoxin of Bacillus thuringiensis israelensis. Mol Gen Genet (1986) 2.01

A 20-kilodalton protein is required for efficient production of the Bacillus thuringiensis subsp. israelensis 27-kilodalton crystal protein in Escherichia coli. J Bacteriol (1989) 1.92

Expression in Escherichia coli of a cloned crystal protein gene of Bacillus thuringiensis subsp. israelensis. J Bacteriol (1987) 1.83

Specificity of action on mosquito larvae of Bacillus thuringiensis israelensis toxins encoded by two different genes. Mol Gen Genet (1988) 1.82

Immunological relationships among proteins making up the Bacillus thuringiensis subsp. israelensis crystalline toxin. Appl Environ Microbiol (1986) 1.82

Separation of the cytolytic and mosquitocidal proteins of Bacillus thuringiensis subsp. israelensis. Biochem Biophys Res Commun (1985) 1.79

Cloning and expression in Escherichia coli of the insecticidal delta-endotoxin gene of Bacillus thuringiensis var. israelensis. FEBS Lett (1984) 1.77

Bacillus thuringiensis var. israelensis delta-endotoxin. Cloning and expression of the toxin in sporogenic and asporogenic strains of Bacillus subtilis. J Mol Biol (1986) 1.74

A Bacillus thuringiensis subsp. israelensis gene encoding a 125-kilodalton larvicidal polypeptide is associated with inverted repeat sequences. J Bacteriol (1988) 1.60

Amplification of a chromosomal region in Bacillus subtilis. J Bacteriol (1985) 1.58

Transformation of vegetative cells of Bacillus thuringiensis by plasmid DNA. J Bacteriol (1987) 1.33

Cloning and expression of two homologous genes of Bacillus thuringiensis subsp. israelensis which encode 130-kilodalton mosquitocidal proteins. J Bacteriol (1988) 1.30

Composition and Toxicity of the Inclusion of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (1985) 1.25

[Preparation and properties of antistreptolysin O horse sera]. Ann Inst Pasteur (Paris) (1965) 1.23

Effect of removal of the cytolytic factor of Bacillus thuringiensis subsp. israelensis on mosquito toxicity. Biochem Biophys Res Commun (1986) 1.04

Clostridium bifermentans serovar malaysia: sporulation, biogenesis of inclusion bodies and larvicidal effect on mosquito. Res Microbiol (1991) 0.88

Fractionation of two mosquitocidal activities from alkali-solubilized extracts of Bacillus thuringiensis subspecies israelensis spores and parasporal inclusions. J Invertebr Pathol (1988) 0.80

Articles by these authors

The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature (1997) 33.47

Essential Bacillus subtilis genes. Proc Natl Acad Sci U S A (2003) 14.01

Inhibitory phosphorylation of the APC regulator Hct1 is controlled by the kinase Cdc28 and the phosphatase Cdc14. Curr Biol (1999) 3.96

Salt stress is an environmental signal affecting degradative enzyme synthesis in Bacillus subtilis. J Bacteriol (1995) 3.66

Signal transduction pathway controlling synthesis of a class of degradative enzymes in Bacillus subtilis: expression of the regulatory genes and analysis of mutations in degS and degU. J Bacteriol (1990) 3.57

CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in gram-positive bacteria. Mol Microbiol (1999) 3.36

A late mitotic regulatory network controlling cyclin destruction in Saccharomyces cerevisiae. Mol Biol Cell (1998) 3.18

Deduced polypeptides encoded by the Bacillus subtilis sacU locus share homology with two-component sensor-regulator systems. J Bacteriol (1988) 2.65

The two-component system ArlS-ArlR is a regulator of virulence gene expression in Staphylococcus aureus. Mol Microbiol (2001) 2.57

The sacT gene regulating the sacPA operon in Bacillus subtilis shares strong homology with transcriptional antiterminators. J Bacteriol (1990) 2.40

Biosynthesis of the parasporal inclusion of Bacillus thuringiensis: half-life of its corresponding messenger RNA. Biochimie (1972) 2.37

Cloning and expression of the crystal protein genes from Bacillus thuringiensis strain berliner 1715. EMBO J (1982) 2.34

ClpP of Bacillus subtilis is required for competence development, motility, degradative enzyme synthesis, growth at high temperature and sporulation. Mol Microbiol (1998) 2.20

PRD--a protein domain involved in PTS-dependent induction and carbon catabolite repression of catabolic operons in bacteria. Mol Microbiol (1998) 2.08

Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT. Mol Microbiol (1997) 2.06

MecB of Bacillus subtilis, a member of the ClpC ATPase family, is a pleiotropic regulator controlling competence gene expression and growth at high temperature. Proc Natl Acad Sci U S A (1994) 2.04

The Bacillus subtilis sigL gene encodes an equivalent of sigma 54 from gram-negative bacteria. Proc Natl Acad Sci U S A (1991) 2.03

Characterization of the genes encoding the haemolytic toxin and the mosquitocidal delta-endotoxin of Bacillus thuringiensis israelensis. Mol Gen Genet (1986) 2.01

Characterization of the sacQ genes from Bacillus licheniformis and Bacillus subtilis. J Bacteriol (1987) 2.01

Two different mechanisms mediate catabolite repression of the Bacillus subtilis levanase operon. J Bacteriol (1995) 1.95

Role of bkdR, a transcriptional activator of the sigL-dependent isoleucine and valine degradation pathway in Bacillus subtilis. J Bacteriol (1999) 1.94

Construction of a colony bank of E. coli containing hybrid plasmids representative of the Bacillus subtilis 168 genome. Expression of functions harbored by the recombinant plasmids in B. subtilis. Mol Gen Genet (1979) 1.94

DegS-DegU and ComP-ComA modulator-effector pairs control expression of the Bacillus subtilis pleiotropic regulatory gene degQ. J Bacteriol (1991) 1.92

Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol (1990) 1.91

RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators. J Bacteriol (1994) 1.91

Specificity of action on mosquito larvae of Bacillus thuringiensis israelensis toxins encoded by two different genes. Mol Gen Genet (1988) 1.82

Induction and metabolite regulation of levanase synthesis in Bacillus subtilis. J Bacteriol (1989) 1.81

Expression of cryIVA and cryIVB Genes, Independently or in Combination, in a Crystal-Negative Strain of Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (1993) 1.79

The transcriptional regulator LevR of Bacillus subtilis has domains homologous to both sigma 54- and phosphotransferase system-dependent regulators. Proc Natl Acad Sci U S A (1991) 1.76

ClpE, a novel type of HSP100 ATPase, is part of the CtsR heat shock regulon of Bacillus subtilis. Mol Microbiol (1999) 1.75

The phosphorylation state of the DegU response regulator acts as a molecular switch allowing either degradative enzyme synthesis or expression of genetic competence in Bacillus subtilis. J Biol Chem (1992) 1.73

Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis. J Mol Biol (1995) 1.67

Mutational analysis of the Bacillus subtilis DegU regulator and its phosphorylation by the DegS protein kinase. J Bacteriol (1991) 1.63

The CtsR regulator of stress response is active as a dimer and specifically degraded in vivo at 37 degrees C. Mol Microbiol (2000) 1.61

Search for supersymmetry using final states with one lepton, jets, and missing transverse momentum with the ATLAS detector in √s=7 TeV pp collisions. Phys Rev Lett (2011) 1.60

A Bacillus thuringiensis subsp. israelensis gene encoding a 125-kilodalton larvicidal polypeptide is associated with inverted repeat sequences. J Bacteriol (1988) 1.60

Mutagenesis of the Bacillus subtilis "-12, -24" promoter of the levanase operon and evidence for the existence of an upstream activating sequence. J Mol Biol (1992) 1.58

Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase. Mol Gen Genet (1987) 1.58

A transposon-like structure related to the delta-endotoxin gene of Bacillus thuringiensis. EMBO J (1984) 1.57

Bacillus subtilis sucrose-specific enzyme II of the phosphotransferase system: expression in Escherichia coli and homology to enzymes II from enteric bacteria. Proc Natl Acad Sci U S A (1987) 1.56

Transcriptional regulation of the cryIVD gene operon from Bacillus thuringiensis subsp. israelensis. J Bacteriol (1995) 1.45

DNA sequences specifying the transcription of the streptococcal kanamycin resistance gene in Escherichia coli and Bacillus subtilis. Mol Gen Genet (1985) 1.43

Observation of a centrality-dependent dijet asymmetry in lead-lead collisions at sqrt[S(NN)] =2.76 TeV with the ATLAS detector at the LHC. Phys Rev Lett (2010) 1.41

Measurement of dijet azimuthal decorrelations in pp collisions at sqrt(s)=7  TeV. Phys Rev Lett (2011) 1.39

Role of the transcriptional activator RocR in the arginine-degradation pathway of Bacillus subtilis. Mol Microbiol (1997) 1.37

Sporulation of Bacillus sphaericus 2297: an electron microscope study of crystal-like inclusion biogenesis and toxicity to mosquito larvae. J Gen Microbiol (1984) 1.36

Molecular cloning, sequencing, and physiological characterization of the qox operon from Bacillus subtilis encoding the aa3-600 quinol oxidase. J Biol Chem (1992) 1.36

Binding of Bacillus sphaericus binary toxin to a specific receptor on midgut brush-border membranes from mosquito larvae. Eur J Biochem (1992) 1.35

Determination of the strange-quark density of the proton from ATLAS measurements of the W→ℓν and Z→ℓℓ cross sections. Phys Rev Lett (2012) 1.34

Regulation of the sacPA operon of Bacillus subtilis: identification of phosphotransferase system components involved in SacT activity. J Bacteriol (1992) 1.32

Search for new particles in two-jet final states in 7 TeV proton-proton collisions with the ATLAS detector at the LHC. Phys Rev Lett (2010) 1.32

Nucleotide sequence and characterization of a new insertion element, IS240, from Bacillus thuringiensis israelensis. Plasmid (1989) 1.32

Resistance in a laboratory population of Culex quinquefasciatus (Diptera: Culicidae) to Bacillus sphaericus binary toxin is due to a change in the receptor on midgut brush-border membranes. Eur J Biochem (1995) 1.31

Nucleotide sequence of the sucrase gene of Bacillus subtilis. Gene (1986) 1.30

Observation of associated near-side and away-side long-range correlations in sqrt[s(NN)]=5.02 TeV proton-lead collisions with the ATLAS detector. Phys Rev Lett (2013) 1.27

Characterization of a novel member of the DegS-DegU regulon affected by salt stress in Bacillus subtilis. J Bacteriol (1998) 1.27

Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis. J Bacteriol (2001) 1.27

The HPr protein of the phosphotransferase system links induction and catabolite repression of the Bacillus subtilis levanase operon. J Bacteriol (1995) 1.26

Antagonistic effects of dual PTS-catalysed phosphorylation on the Bacillus subtilis transcriptional activator LevR. Mol Microbiol (1998) 1.24

Search for dark matter candidates and large extra dimensions in events with a photon and missing transverse momentum in pp collision data at sqrt[s]=7 TeV with the ATLAS detector. Phys Rev Lett (2013) 1.19

Cloning and expression in Escherichia coli of the regulatory sacU gene from Bacillus subtilis. J Bacteriol (1985) 1.14

Search for a supersymmetric partner to the top quark in final states with jets and missing transverse momentum at sqrt[s] = 7 TeV with the ATLAS detector. Phys Rev Lett (2012) 1.13

New serovars of Bacillus thuringiensis: B. thuringiensis ser. coreanensis (serotype H25), B. thuringiensis ser. leesis (serotype H33), and B. thuringiensis ser. konkukian (serotype H34). J Invertebr Pathol (1994) 1.13

Improvement of Bacillus sphaericus toxicity against dipteran larvae by integration, via homologous recombination, of the Cry11A toxin gene from Bacillus thuringiensis subsp. israelensis. Appl Environ Microbiol (1997) 1.13

The receptor of Bacillus sphaericus binary toxin in Culex pipiens (Diptera: Culicidae) midgut: molecular cloning and expression. Insect Biochem Mol Biol (2001) 1.13

[Electron microscope study of "Bacillus thuringiensis" var. "Israelensis" sporulation and crystal biogenesis (author's transl)]. Ann Microbiol (Paris) (1982) 1.12

In vitro reconstitution of transcriptional antitermination by the SacT and SacY proteins of Bacillus subtilis. J Biol Chem (1996) 1.12

The DegS/DegU and ComP/ComA two-component systems are part of a network controlling degradative enzyme synthesis and competence in Bacillus subtilis. Res Microbiol (1995) 1.12

Interactions of wild-type and truncated LevR of Bacillus subtilis with the upstream activating sequence of the levanase operon. J Mol Biol (1994) 1.12

Characterization of an intracellular protease isolated from Bacillus thuringiensis sporulating cells and able to modify homologous RNA polymerase. Eur J Biochem (1977) 1.10

Cloning and expression in Escherichia coli of the sucrase gene from Bacillus subtilis. Mol Gen Genet (1982) 1.10