PubRank

Ben Shen

Author PubWeight™ 158.27‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature. Nat Prod Rep 2013 5.38
2 Inhibition of eukaryotic translation elongation by cycloheximide and lactimidomycin. Nat Chem Biol 2010 3.44
3 Decoding human cytomegalovirus. Science 2012 2.98
4 Global mapping of translation initiation sites in mammalian cells at single-nucleotide resolution. Proc Natl Acad Sci U S A 2012 2.79
5 Biosynthesis of the enediyne antitumor antibiotic C-1027. Science 2002 2.53
6 A genomics-guided approach for discovering and expressing cryptic metabolic pathways. Nat Biotechnol 2003 2.41
7 Type I polyketide synthase requiring a discrete acyltransferase for polyketide biosynthesis. Proc Natl Acad Sci U S A 2003 2.40
8 Thiopeptide biosynthesis featuring ribosomally synthesized precursor peptides and conserved posttranslational modifications. Chem Biol 2009 1.93
9 Antitumor antibiotics: bleomycin, enediynes, and mitomycin. Chem Rev 2005 1.91
10 Leinamycin biosynthesis revealing unprecedented architectural complexity for a hybrid polyketide synthase and nonribosomal peptide synthetase. Chem Biol 2004 1.89
11 Microbial genomics for the improvement of natural product discovery. Curr Opin Microbiol 2006 1.87
12 Characterization of the maduropeptin biosynthetic gene cluster from Actinomadura madurae ATCC 39144 supporting a unifying paradigm for enediyne biosynthesis. J Am Chem Soc 2007 1.69
13 The neocarzinostatin biosynthetic gene cluster from Streptomyces carzinostaticus ATCC 15944 involving two iterative type I polyketide synthases. Chem Biol 2005 1.61
14 Nosiheptide biosynthesis featuring a unique indole side ring formation on the characteristic thiopeptide framework. ACS Chem Biol 2009 1.56
15 Rapid PCR amplification of minimal enediyne polyketide synthase cassettes leads to a predictive familial classification model. Proc Natl Acad Sci U S A 2003 1.48
16 Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus. J Am Chem Soc 2005 1.46
17 A novel 4-methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic C-1027 biosynthesis. J Am Chem Soc 2003 1.42
18 Biosynthesis of enediyne antitumor antibiotics. Curr Top Med Chem 2008 1.39
19 Designer enediynes generate DNA breaks, interstrand cross-links, or both, with concomitant changes in the regulation of DNA damage responses. Proc Natl Acad Sci U S A 2007 1.38
20 Identification and localization of the gene cluster encoding biosynthesis of the antitumor macrolactam leinamycin in Streptomyces atroolivaceus S-140. J Bacteriol 2002 1.38
21 A phosphopantetheinylating polyketide synthase producing a linear polyene to initiate enediyne antitumor antibiotic biosynthesis. Proc Natl Acad Sci U S A 2008 1.36
22 Single chemical modifications of the C-1027 enediyne core, a radiomimetic antitumor drug, affect both drug potency and the role of ataxia-telangiectasia mutated in cellular responses to DNA double-strand breaks. Cancer Res 2007 1.31
23 Iso-migrastatin congeners from Streptomyces platensis and generation of a glutarimide polyketide library featuring the dorrigocin, lactimidomycin, migrastatin, and NK30424 scaffolds. J Am Chem Soc 2005 1.25
24 Migrastatin and dorrigocins are shunt metabolites of iso-migrastatin. J Am Chem Soc 2005 1.24
25 C-O bond formation by polyketide synthases. Science 2002 1.23
26 Engineered Streptomyces platensis strains that overproduce antibiotics platensimycin and platencin. Antimicrob Agents Chemother 2009 1.23
27 The role of tandem acyl carrier protein domains in polyunsaturated fatty acid biosynthesis. J Am Chem Soc 2008 1.21
28 The tallysomycin biosynthetic gene cluster from Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insights into the biosynthesis of the bleomycin family of antitumor antibiotics. Mol Biosyst 2006 1.20
29 Improvement of secondary metabolite production in Streptomyces by manipulating pathway regulation. Appl Microbiol Biotechnol 2010 1.19
30 Kinetic analysis of the 4-methylideneimidazole-5-one-containing tyrosine aminomutase in enediyne antitumor antibiotic C-1027 biosynthesis. Biochemistry 2003 1.16
31 Bacterial diterpene synthases: new opportunities for mechanistic enzymology and engineered biosynthesis. Curr Opin Chem Biol 2012 1.15
32 Oxidase domains in epothilone and bleomycin biosynthesis: thiazoline to thiazole oxidation during chain elongation. Biochemistry 2003 1.15
33 Dedicated ent-kaurene and ent-atiserene synthases for platensimycin and platencin biosynthesis. Proc Natl Acad Sci U S A 2011 1.15
34 Biosynthesis of the beta-amino acid moiety of the enediyne antitumor antibiotic C-1027 featuring beta-amino acyl-S-carrier protein intermediates. J Am Chem Soc 2005 1.14
35 The bifunctional glyceryl transferase/phosphatase OzmB belonging to the HAD superfamily that diverts 1,3-bisphosphoglycerate into polyketide biosynthesis. J Am Chem Soc 2006 1.13
36 Polyketide synthase chemistry does not direct biosynthetic divergence between 9- and 10-membered enediynes. Proc Natl Acad Sci U S A 2010 1.13
37 Tirandamycins from Streptomyces sp. 17944 inhibiting the parasite Brugia malayi asparagine tRNA synthetase. Org Lett 2011 1.12
38 Polyketide chain skipping mechanism in the biosynthesis of the hybrid nonribosomal peptide-polyketide antitumor antibiotic leinamycin in Streptomyces atroolivaceus S-140. J Nat Prod 2006 1.11
39 Engineered production of iso-migrastatin in heterologous Streptomyces hosts. Bioorg Med Chem 2008 1.11
40 Regiospecific chlorination of (S)-beta-tyrosyl-S-carrier protein catalyzed by SgcC3 in the biosynthesis of the enediyne antitumor antibiotic C-1027. J Am Chem Soc 2007 1.11
41 A free-standing condensation enzyme catalyzing ester bond formation in C-1027 biosynthesis. Proc Natl Acad Sci U S A 2009 1.11
42 Expression of biosynthetic gene clusters in heterologous hosts for natural product production and combinatorial biosynthesis. Expert Opin Drug Discov 2006 1.10
43 Radical-mediated enzymatic carbon chain fragmentation-recombination. Nat Chem Biol 2011 1.09
44 Evaluation of new migrastatin and dorrigocin congeners unveils cell migration inhibitors with dramatically improved potency. Bioorg Med Chem Lett 2008 1.08
45 iso-Migrastatin, migrastatin, and dorrigocin production in Streptomyces platensis NRRL 18993 is governed by a single biosynthetic machinery featuring an acyltransferase-less type I polyketide synthase. J Biol Chem 2009 1.08
46 The structure of L-tyrosine 2,3-aminomutase from the C-1027 enediyne antitumor antibiotic biosynthetic pathway. Biochemistry 2007 1.08
47 Identification and utility of FdmR1 as a Streptomyces antibiotic regulatory protein activator for fredericamycin production in Streptomyces griseus ATCC 49344 and heterologous hosts. J Bacteriol 2008 1.07
48 Signal mimics derived from a metagenomic analysis of the gypsy moth gut microbiota. Appl Environ Microbiol 2007 1.06
49 Characterization of intact antibody-drug conjugates from plasma/serum in vivo by affinity capture capillary liquid chromatography-mass spectrometry. Anal Biochem 2011 1.06
50 Tautomycetin and tautomycin suppress the growth of medullary thyroid cancer cells via inhibition of glycogen synthase kinase-3beta. Mol Cancer Ther 2009 1.05
51 Engineering of Streptomyces platensis MA7339 for overproduction of platencin and congeners. Org Lett 2010 1.05
52 Lactimidomycin, iso-migrastatin and related glutarimide-containing 12-membered macrolides are extremely potent inhibitors of cell migration. J Am Chem Soc 2009 1.04
53 Conversion of (2S)-arginine to (2S,3R)-capreomycidine by VioC and VioD from the viomycin biosynthetic pathway of Streptomyces sp. strain ATCC11861. Chembiochem 2004 1.04
54 Strain prioritization for natural product discovery by a high-throughput real-time PCR method. J Nat Prod 2014 1.03
55 Chain initiation in the leinamycin-producing hybrid nonribosomal peptide/polyketide synthetase from Streptomyces atroolivaceus S-140. Discrete, monofunctional adenylation enzyme and peptidyl carrier protein that directly load D-alanine. J Biol Chem 2007 1.03
56 The biosynthetic gene cluster of zorbamycin, a member of the bleomycin family of antitumor antibiotics, from Streptomyces flavoviridis ATCC 21892. Mol Biosyst 2008 1.03
57 Characterization of the tautomycetin biosynthetic gene cluster from Streptomyces griseochromogenes provides new insight into dialkylmaleic anhydride biosynthesis. J Nat Prod 2009 1.03
58 Biosynthetic potential-based strain prioritization for natural product discovery: a showcase for diterpenoid-producing actinomycetes. J Nat Prod 2014 1.02
59 New lactimidomycin congeners shed insight into lactimidomycin biosynthesis in Streptomyces amphibiosporus. Org Lett 2007 1.02
60 C-1027, a radiomimetic enediyne anticancer drug, preferentially targets hypoxic cells. Cancer Res 2009 1.02
61 Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism. Proc Natl Acad Sci U S A 2008 1.01
62 Actinopolysporins A-C and tubercidin as a Pdcd4 stabilizer from the halophilic actinomycete Actinopolyspora erythraea YIM 90600. J Nat Prod 2011 1.00
63 Bafilomycins produced by an endophytic actinomycete Streptomyces sp. YIM56209. J Antibiot (Tokyo) 2010 1.00
64 Advances in polyketide synthase structure and function. Curr Opin Drug Discov Devel 2008 1.00
65 Substrate specificity of the adenylation enzyme SgcC1 involved in the biosynthesis of the enediyne antitumor antibiotic C-1027. J Biol Chem 2006 1.00
66 Identification and characterization of a novel estrogenic ligand actinopolymorphol A. Biochem Pharmacol 2010 0.99
67 Type I polyketide synthases that require discrete acyltransferases. Methods Enzymol 2009 0.99
68 BlmIII and BlmIV nonribosomal peptide synthetase-catalyzed biosynthesis of the bleomycin bithiazole moiety involving both in cis and in trans aminoacylation. Biochemistry 2003 0.99
69 SHP2 is a target of the immunosuppressant tautomycetin. Chem Biol 2011 0.98
70 Characterization of the two-component, FAD-dependent monooxygenase SgcC that requires carrier protein-tethered substrates for the biosynthesis of the enediyne antitumor antibiotic C-1027. J Am Chem Soc 2008 0.97
71 Bifunctional acyltransferase/decarboxylase LnmK as the missing link for beta-alkylation in polyketide biosynthesis. J Am Chem Soc 2009 0.97
72 Comparative analysis of the biosynthetic gene clusters and pathways for three structurally related antitumor antibiotics: bleomycin, tallysomycin, and zorbamycin. J Nat Prod 2011 0.96
73 In vivo investigation of the roles of FdmM and FdmM1 in fredericamycin biosynthesis unveiling a new family of oxygenases. J Biol Chem 2009 0.96
74 Characterization of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus unveiling new insights into dialkylmaleic anhydride and polyketide biosynthesis. J Biol Chem 2008 0.96
75 Cloning and sequencing of the kedarcidin biosynthetic gene cluster from Streptoalloteichus sp. ATCC 53650 revealing new insights into biosynthesis of the enediyne family of antitumor antibiotics. Mol Biosyst 2013 0.96
76 Thermolysis of isomigrastatin and its congeners via [3,3]-sigmatropic rearrangement: a new route to the synthesis of migrastatin and its analogues. Org Lett 2006 0.95
77 Erythronolides H and I, new erythromycin congeners from a new halophilic actinomycete Actinopolyspora sp. YIM90600. Org Lett 2009 0.95
78 Characterization of the SgcF epoxide hydrolase supporting an (R)-vicinal diol intermediate for enediyne antitumor antibiotic C-1027 biosynthesis. J Am Chem Soc 2009 0.94
79 Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning of the tautomycin biosynthetic gene cluster from Streptomyces spiroverticillatus. J Bacteriol 2006 0.93
80 Iterative type I polyketide synthases for enediyne core biosynthesis. Methods Enzymol 2009 0.93
81 Tautomycin suppresses growth and neuroendocrine hormone markers in carcinoid cells through activation of the Raf-1 pathway. Am J Surg 2009 0.93
82 Molecular basis of substrate promiscuity for the SAM-dependent O-methyltransferase NcsB1, involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin. Biochemistry 2009 0.93
83 A dedicated phosphopantetheinyl transferase for the fredericamycin polyketide synthase from Streptomyces griseus. J Biol Chem 2006 0.92
84 Structure of the bifunctional acyltransferase/decarboxylase LnmK from the leinamycin biosynthetic pathway revealing novel activity for a double-hot-dog fold. Biochemistry 2013 0.92
85 Characterization of NcsB2 as a promiscuous naphthoic acid/coenzyme A ligase integral to the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin. J Am Chem Soc 2007 0.92
86 Functional characterization of TtnD and TtnF, unveiling new insights into tautomycetin biosynthesis. J Am Chem Soc 2010 0.92
87 Glycopeptide antitumor antibiotic zorbamycin from Streptomyces flavoviridis ATCC 21892: strain improvement and structure elucidation. J Nat Prod 2007 0.91
88 New WS9326A congeners from Streptomyces sp. 9078 inhibiting Brugia malayi asparaginyl-tRNA synthetase. Org Lett 2012 0.91
89 The crystal structure of BlmI as a model for nonribosomal peptide synthetase peptidyl carrier proteins. Proteins 2014 0.91
90 Oxazolomycin biosynthesis in Streptomyces albus JA3453 featuring an "acyltransferase-less" type I polyketide synthase that incorporates two distinct extender units. J Biol Chem 2010 0.91
91 Hybrid peptide-polyketide natural products: biosynthesis and prospects towards engineering novel molecules. Genet Eng (N Y) 2003 0.90
92 NosA catalyzing carboxyl-terminal amide formation in nosiheptide maturation via an enamine dealkylation on the serine-extended precursor peptide. J Am Chem Soc 2010 0.90
93 Cycloheximide and congeners as inhibitors of eukaryotic protein synthesis from endophytic actinomycetes Streptomyces sps. YIM56132 and YIM56141. J Antibiot (Tokyo) 2010 0.89
94 Discovery and total synthesis of a new estrogen receptor heterodimerizing actinopolymorphol A from Actinopolymorpha rutilus. Org Lett 2010 0.89
95 Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning the oxazolomycin biosynthetic gene cluster from Streptomyces albus JA3453. J Bacteriol 2006 0.89
96 Identification of fredericamycin E from Streptomyces griseus: Insights into fredericamycin A biosynthesis highlighting carbaspirocycle formation. J Nat Prod 2008 0.89
97 A genetically amenable platensimycin- and platencin-overproducer as a platform for biosynthetic explorations: a showcase of PtmO4, a long-chain acyl-CoA dehydrogenase. Mol Biosyst 2015 0.89
98 Isolation and structural elucidation of glucoside congeners of platencin from Streptomyces platensis SB12600. J Antibiot (Tokyo) 2013 0.88
99 Biochemical characterization of the SgcA1 alpha-D-glucopyranosyl-1-phosphate thymidylyltransferase from the enediyne antitumor antibiotic C-1027 biosynthetic pathway and overexpression of sgcA1 in Streptomyces globisporus to improve C-1027 production. J Nat Prod 2004 0.88
100 In vivo manipulation of the bleomycin biosynthetic gene cluster in Streptomyces verticillus ATCC15003 revealing new insights into its biosynthetic pathway. J Biol Chem 2008 0.88
101 Characterization of the lnmKLM genes unveiling key intermediates for β-alkylation in leinamycin biosynthesis. Org Lett 2010 0.88
102 Expression of the platencin biosynthetic gene cluster in heterologous hosts yielding new platencin congeners. J Nat Prod 2012 0.87
103 Functional characterization of ttmM unveils new tautomycin analogs and insight into tautomycin biosynthesis and activity. Org Lett 2009 0.87
104 Possible steps of complete disassembly of post-termination complex by yeast eEF3 deduced from inhibition by translocation inhibitors. Nucleic Acids Res 2012 0.86
105 The mechanism of MIO-based aminomutases in beta-amino acid biosynthesis. J Am Chem Soc 2007 0.86
106 Regiospecific O-methylation of naphthoic acids catalyzed by NcsB1, an O-methyltransferase involved in the biosynthesis of the enediyne antitumor antibiotic neocarzinostatin. J Biol Chem 2008 0.86
107 Natural product drug discovery: the times have never been better. Chem Biol 2007 0.85
108 Improvement of the enediyne antitumor antibiotic C-1027 production by manipulating its biosynthetic pathway regulation in Streptomyces globisporus. J Nat Prod 2011 0.85
109 Multifaceted modes of action for the glutarimide-containing polyketides revealed. Chembiochem 2010 0.85
110 Titer improvement of iso-migrastatin in selected heterologous Streptomyces hosts and related analysis of mRNA expression by quantitative RT-PCR. Appl Microbiol Biotechnol 2010 0.85
111 The missing C-17 O-methyltransferase in geldanamycin biosynthesis. Org Lett 2011 0.84
112 4-methylideneimidazole-5-one-containing aminomutases in enediyne biosynthesis. Methods Enzymol 2012 0.84
113 Manipulation of pathway regulation in Streptomyces globisporus for overproduction of the enediyne antitumor antibiotic C-1027. J Antibiot (Tokyo) 2010 0.84
114 Functional characterization of tlmH in Streptoalloteichus hindustanus E465-94 ATCC 31158 unveiling new insight into tallysomycin biosynthesis and affording a novel bleomycin analog. Mol Biosyst 2009 0.83
115 Comparative characterization of the lactimidomycin and iso-migrastatin biosynthetic machineries revealing unusual features for acyltransferase-less type I polyketide synthases and providing an opportunity to engineer new analogues. Biochemistry 2014 0.83
116 Characterization of FdmV as an amide synthetase for fredericamycin A biosynthesis in Streptomyces griseus ATCC 43944. J Biol Chem 2010 0.83
117 A designer bleomycin with significantly improved DNA cleavage activity. J Am Chem Soc 2012 0.83
118 Development of a chemically defined medium for the production of the antibiotic platensimycin by Streptomyces platensis. Appl Microbiol Biotechnol 2013 0.82
119 Angucyclines and Angucyclinones from Streptomyces sp. CB01913 Featuring C-Ring Cleavage and Expansion. J Nat Prod 2015 0.82
120 Design and characterization of mechanism-based inhibitors for the tyrosine aminomutase SgTAM. Bioorg Med Chem Lett 2007 0.82
121 Neaumycin: a new macrolide from Streptomyces sp. NEAU-x211. Org Lett 2012 0.82
122 A proteogenomics approach integrating proteomics and ribosome profiling increases the efficiency of protein identification and enables the discovery of alternative translation start sites. Proteomics 2014 0.82
123 Identification of antifungal natural products via Saccharomyces cerevisiae bioassay: insights into macrotetrolide drug spectrum, potency and mode of action. Med Mycol 2012 0.82
124 Tailoring enzymes acting on carrier protein-tethered substrates in natural product biosynthesis. Methods Enzymol 2012 0.82
125 Cycloheximide and actiphenol production in Streptomyces sp. YIM56141 governed by single biosynthetic machinery featuring an acyltransferase-less type I polyketide synthase. Org Lett 2014 0.80
126 Enediyne antitumor antibiotic maduropeptin biosynthesis featuring a C-methyltransferase that acts on a CoA-tethered aromatic substrate. J Am Chem Soc 2010 0.80
127 Characterization of the epoxide hydrolase NcsF2 from the neocarzinostatin biosynthetic gene cluster. Org Lett 2010 0.80
128 Functional characterization of tlmK unveiling unstable carbinolamide intermediates in the tallysomycin biosynthetic pathway. J Biol Chem 2009 0.80
129 Real-time quantification of antibody-short interfering RNA conjugate in serum by antigen capture reverse transcription-polymerase chain reaction. Anal Biochem 2012 0.80
130 Characterization of SgcE6, the flavin reductase component supporting FAD-dependent halogenation and hydroxylation in the biosynthesis of the enediyne antitumor antibiotic C-1027. FEMS Microbiol Lett 2009 0.80
131 Functional characterization of ttnI completing the tailoring steps for tautomycetin biosynthesis in Streptomyces griseochromogenes. Org Lett 2012 0.80
132 BlmB and TlmB provide resistance to the bleomycin family of antitumor antibiotics by N-acetylating metal-free bleomycin, tallysomycin, phleomycin, and zorbamycin. Biochemistry 2014 0.79
133 Specificity of the ester bond forming condensation enzyme SgcC5 in C-1027 biosynthesis. Org Lett 2012 0.79
134 Post-polyketide synthase steps in iso-migrastatin biosynthesis, featuring tailoring enzymes with broad substrate specificity. J Am Chem Soc 2013 0.78
135 Enediyne polyketide synthases stereoselectively reduce the β-ketoacyl intermediates to β-D-hydroxyacyl intermediates in enediyne core biosynthesis. Org Lett 2014 0.78
136 Platensimycin and platencin biosynthesis in Streptomyces platensis, showcasing discovery and characterization of novel bacterial diterpene synthases. Methods Enzymol 2012 0.78
137 Alternative method for site-directed mutagenesis of complex polyketide synthase in Streptomyces albus JA3453. Acta Biochim Biophys Sin (Shanghai) 2008 0.78
138 Improved production of the tallysomycin H-1 in Streptoalloteichus hindustanus SB8005 strain by fermentation optimization. Appl Microbiol Biotechnol 2010 0.77
139 A new member of the 4-methylideneimidazole-5-one-containing aminomutase family from the enediyne kedarcidin biosynthetic pathway. Proc Natl Acad Sci U S A 2013 0.76
140 Iso-migrastatin Titer Improvement in the Engineered Streptomyces lividans SB11002 Strain by Optimization of Fermentation Conditions. Biotechnol Bioprocess Eng 2010 0.76
141 Expanding nature's chemical repertoire through metabolic engineering and biocatalysis. Curr Opin Chem Biol 2012 0.76
142 Special issue of The Journal of Antibiotics dedicated to the late Professor C Richard Hutchinson. J Antibiot (Tokyo) 2011 0.76
143 Predictive model for epoxide hydrolase-generated stereochemistry in the biosynthesis of nine-membered enediyne antitumor antibiotics. Biochemistry 2013 0.76
144 [Experimental research and analysis on dielectric properties of blood in anemia mice]. Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2013 0.75
145 Tandem acyl carrier protein domains in polyunsaturated fatty acid synthases. Methods Enzymol 2009 0.75