A protocol for phenotypic detection and enumeration of circulating endothelial cells and circulating progenitor cells in human blood.

PubWeight™: 2.59‹?› | Rank: Top 1%

🔗 View Article (PMC 2686125)

Published in Nat Protoc on January 01, 2007

Authors

Dan G Duda1, Kenneth S Cohen, David T Scadden, Rakesh K Jain

Author Affiliations

1: Steele Laboratory for Tumor Biology, Department of Radiation Oncology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.

Associated clinical trials:

Neuromuscular Electrical Stimulation and Septic Shock | NCT03193164

Articles citing this

Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study. J Clin Oncol (2009) 4.54

Tumor microvasculature and microenvironment: targets for anti-angiogenesis and normalization. Microvasc Res (2007) 3.64

Phase II study of cediranib, an oral pan-vascular endothelial growth factor receptor tyrosine kinase inhibitor, in patients with recurrent glioblastoma. J Clin Oncol (2010) 3.43

Efficacy, safety, and biomarkers of neoadjuvant bevacizumab, radiation therapy, and fluorouracil in rectal cancer: a multidisciplinary phase II study. J Clin Oncol (2009) 3.39

Bone marrow-derived circulating endothelial precursors do not contribute to vascular endothelium and are not needed for tumor growth. Proc Natl Acad Sci U S A (2008) 3.27

Endothelial progenitor cells: identity defined? J Cell Mol Med (2009) 2.96

Human cardiomyocyte progenitor cells differentiate into functional mature cardiomyocytes: an in vitro model for studying human cardiac physiology and pathophysiology. Nat Protoc (2009) 2.16

Flow cytometric identification and functional characterization of immature and mature circulating endothelial cells. Arterioscler Thromb Vasc Biol (2012) 2.07

Human endothelial progenitor cells. Cold Spring Harb Perspect Med (2012) 1.68

VEGF-targeted cancer therapy strategies: current progress, hurdles and future prospects. Trends Mol Med (2007) 1.64

Bone marrow-derived endothelial progenitor cells contribute to the angiogenic switch in tumor growth and metastatic progression. Biochim Biophys Acta (2009) 1.54

Rare cell isolation and analysis in microfluidics. Lab Chip (2014) 1.42

Circulating endothelial cells, microparticles and progenitors: key players towards the definition of vascular competence. J Cell Mol Med (2009) 1.35

Endothelial progenitor cells and vascular dysfunction in children with obstructive sleep apnea. Am J Respir Crit Care Med (2010) 1.32

Antiangiogenics: the potential role of integrating this novel treatment modality with chemoradiation for solid cancers. J Clin Oncol (2007) 1.23

Application of polychromatic flow cytometry to identify novel subsets of circulating cells with angiogenic potential. Cytometry A (2010) 1.22

Endothelial abnormalities in adolescents with type 1 diabetes: a biomarker for vascular sequelae? J Pediatr (2010) 1.18

Oxidative stress impairs endothelial progenitor cell function. Antioxid Redox Signal (2008) 1.15

Vitamin D improves the angiogenic properties of endothelial progenitor cells. Am J Physiol Cell Physiol (2012) 1.11

The definition of EPCs and other bone marrow cells contributing to neoangiogenesis and tumor growth: is there common ground for understanding the roles of numerous marrow-derived cells in the neoangiogenic process? Biochim Biophys Acta (2009) 1.11

New molecular targets in angiogenic vessels of glioblastoma tumours. Expert Rev Mol Med (2008) 1.08

Circulating CD34(+) progenitor cell frequency is associated with clinical and genetic factors. Blood (2013) 1.07

Human endothelial stem/progenitor cells, angiogenic factors and vascular repair. J R Soc Interface (2010) 1.04

Effects of sorafenib on intra-tumoral interstitial fluid pressure and circulating biomarkers in patients with refractory sarcomas (NCI protocol 6948). PLoS One (2012) 1.03

Phase I trial with biomarker studies of vatalanib (PTK787) in patients with newly diagnosed glioblastoma treated with enzyme inducing anti-epileptic drugs and standard radiation and temozolomide. J Neurooncol (2010) 1.02

Phase II study of neoadjuvant bevacizumab and radiotherapy for resectable soft tissue sarcomas. Int J Radiat Oncol Biol Phys (2010) 1.02

Influence of BMI on level of circulating progenitor cells. Obesity (Silver Spring) (2011) 0.99

Gestational diabetes mellitus alters maternal and neonatal circulating endothelial progenitor cell subsets. Am J Obstet Gynecol (2010) 0.97

Late release of circulating endothelial cells and endothelial progenitor cells after chemotherapy predicts response and survival in cancer patients. Neoplasia (2010) 0.97

Exploratory analysis of early toxicity of sunitinib in advanced hepatocellular carcinoma patients: kinetics and potential biomarker value. Clin Cancer Res (2010) 0.96

Circulating CD34+ cell subsets in patients with coronary endothelial dysfunction. Nat Clin Pract Cardiovasc Med (2008) 0.96

Quantification of circulating endothelial and progenitor cells: comparison of quantitative PCR and four-channel flow cytometry. BMC Res Notes (2008) 0.94

Stromal cell-derived factor 1 as a biomarker of heart failure and mortality risk. Arterioscler Thromb Vasc Biol (2014) 0.93

Complete pathological response to bevacizumab and chemoradiation in advanced rectal cancer. Nat Clin Pract Oncol (2007) 0.92

Quantity and clinical relevance of circulating endothelial progenitor cells in human ovarian cancer. J Exp Clin Cancer Res (2010) 0.90

Antiangiogenic therapy for glioma. J Signal Transduct (2012) 0.89

A phase 1/2 and biomarker study of preoperative short course chemoradiation with proton beam therapy and capecitabine followed by early surgery for resectable pancreatic ductal adenocarcinoma. Int J Radiat Oncol Biol Phys (2014) 0.87

Endothelial colony-forming cells derived from pregnancies complicated by intrauterine growth restriction are fewer and have reduced vasculogenic capacity. J Clin Endocrinol Metab (2013) 0.87

Safety, Efficacy, and Biomarker Exploration in a Phase II Study of Bevacizumab, Oxaliplatin, and Gemcitabine in Recurrent Müllerian Carcinoma. Clin Ovarian Cancer Other Gynecol Malig (2011) 0.87

High-density lipoprotein exerts vasculoprotection via endothelial progenitor cells. J Cell Mol Med (2010) 0.87

Biology and flow cytometry of proangiogenic hematopoietic progenitors cells. Cytometry A (2014) 0.84

A pilot study using dynamic contrast enhanced-MRI as a response biomarker of the radioprotective effect of memantine in patients receiving whole brain radiotherapy. Oncotarget (2016) 0.84

Design and validation of an endothelial progenitor cell capture chip and its application in patients with pulmonary arterial hypertension. J Mol Med (Berl) (2011) 0.83

Circulating endothelial cells and circulating endothelial precursor cells in patients with osteosarcoma. Pediatr Blood Cancer (2011) 0.83

Association of colony-forming units with coronary artery and abdominal aortic calcification. Circulation (2010) 0.83

Cord blood endothelial progenitor cells as therapeutic and imaging probes. Imaging Med (2012) 0.82

Regeneration of the vascular compartment. Herz (2010) 0.80

Mobilization of circulating endothelial progenitor cells correlates with the clinical course of hantavirus disease. J Virol (2013) 0.80

Cell-based therapy in lung regenerative medicine. Regen Med Res (2014) 0.80

Endothelial progenitor cells are differentially impaired in ANCA-associated vasculitis compared to healthy controls. Arthritis Res Ther (2016) 0.79

Are Endothelial Progenitor Cells the Real Solution for Cardiovascular Diseases? Focus on Controversies and Perspectives. Biomed Res Int (2015) 0.79

Fetal exposure to a diabetic intrauterine environment resulted in a failure of cord blood endothelial progenitor cell adaptation against chronic hypoxia. Stem Cells Cloning (2014) 0.79

Effects of acute exercise on circulating endothelial and progenitor cells in children and adolescents with juvenile idiopathic arthritis and healthy controls: a pilot study. Pediatr Rheumatol Online J (2015) 0.79

Circulating CD31+ leukocyte frequency is associated with cardiovascular risk factors. Atherosclerosis (2013) 0.79

Histone deacetylase (HDAC) inhibitors down-regulate endothelial lineage commitment of umbilical cord blood derived endothelial progenitor cells. Int J Mol Sci (2012) 0.79

Recombinant human interleukin-11 treatment enhances collateral vessel growth after femoral artery ligation. Arterioscler Thromb Vasc Biol (2010) 0.78

Cholesterol and hematopoietic stem cells: inflammatory mediators of atherosclerosis. Stem Cells Transl Med (2014) 0.78

Circulating angiogenic factors in patients with thromboangiitis obliterans. PLoS One (2012) 0.78

Effects of acute exercise on markers of inflammation in pediatric chronic kidney disease: a pilot study. Pediatr Nephrol (2014) 0.78

VEGF Neutralization Plus CTLA-4 Blockade Alters Soluble and Cellular Factors Associated with Enhancing Lymphocyte Infiltration and Humoral Recognition in Melanoma. Cancer Immunol Res (2016) 0.78

Phase 1 study of combination treatment with PTK 787/ZK 222584 and cetuximab for patients with advanced solid tumors: safety, pharmacokinetics, pharmacodynamics analysis. Neoplasia (2010) 0.78

In vitro endothelialization of electrospun terpolymer scaffolds: evaluation of scaffold type and cell source. Tissue Eng Part A (2012) 0.77

Hidden keys in stroma: Unlocking the tumor progression. J Oral Maxillofac Pathol (2013) 0.77

Circulating angiogenic cell populations, vascular function, and arterial stiffness. Atherosclerosis (2011) 0.77

Endothelial progenitor cells and endothelial vesicles - what is the significance for patients with chronic kidney disease? Blood Purif (2010) 0.77

Biomarkers of evasive resistance predict disease progression in cancer patients treated with antiangiogenic therapies. Oncotarget (2016) 0.76

A module of human peripheral blood mononuclear cell transcriptional network containing primitive and differentiation markers is related to specific cardiovascular health variables. PLoS One (2014) 0.76

Neuromuscular electrical stimulation acutely mobilizes endothelial progenitor cells in critically ill patients with sepsis. Ann Intensive Care (2016) 0.76

A Phase I Clinical, Pharmacokinetic, and Pharmacodynamic Study of Weekly or Every Three Week Ixabepilone and Daily Sunitinib in Patients with Advanced Solid Tumors. Clin Cancer Res (2016) 0.75

Isolation of a circulating CD45-, CD34dim cell population and validation of their endothelial phenotype. Thromb Haemost (2014) 0.75

Evaluation of neural damage in Duchenne muscular dystrophy patients. Acta Myol (2014) 0.75

Endothelial Progenitor Cells of the Human Placenta and Fetoplacental Circulation: A Potential Link to Fetal, Neonatal, and Long-term Health. Front Pediatr (2017) 0.75

Modulation of Endothelial Injury Biomarkers by Traditional Chinese Medicine LC in Systemic Lupus Erythematosus Patients Receiving Standard Treatments. Sci Rep (2016) 0.75

Association of obesity and circulating adipose stromal cells among breast cancer survivors. Mol Biol Rep (2014) 0.75

Cryopreservation and Enumeration of Human Endothelial Progenitor and Endothelial Cells for Clinical Trials. J Blood Disord Transfus (2013) 0.75

The effectiveness of Robot-Assisted Gait Training versus conventional therapy on mobility in severely disabled progressIve MultiplE sclerosis patients (RAGTIME): study protocol for a randomized controlled trial. Trials (2017) 0.75

Distribution of endothelial progenitor cells in tissues from patients with gastric cancer. Oncol Lett (2014) 0.75

Clinical significance of 5-(and 6)-carboxyfluorescein diacetate succinimidyl ester-labeled microspheres for detecting endothelial progenitor cells in human peripheral blood. Exp Ther Med (2017) 0.75

Articles cited by this

AZD2171, a pan-VEGF receptor tyrosine kinase inhibitor, normalizes tumor vasculature and alleviates edema in glioblastoma patients. Cancer Cell (2007) 12.52

Direct evidence that the VEGF-specific antibody bevacizumab has antivascular effects in human rectal cancer. Nat Med (2004) 9.80

Lessons from phase III clinical trials on anti-VEGF therapy for cancer. Nat Clin Pract Oncol (2006) 7.19

Essential role of endothelial nitric oxide synthase for mobilization of stem and progenitor cells. Nat Med (2003) 6.09

Vascular and haematopoietic stem cells: novel targets for anti-angiogenesis therapy? Nat Rev Cancer (2002) 5.02

The multifaceted circulating endothelial cell in cancer: towards marker and target identification. Nat Rev Cancer (2006) 4.55

Surrogate markers for antiangiogenic therapy and dose-limiting toxicities for bevacizumab with radiation and chemotherapy: continued experience of a phase I trial in rectal cancer patients. J Clin Oncol (2005) 3.83

Resting and activated endothelial cells are increased in the peripheral blood of cancer patients. Blood (2001) 3.24

Differential CD146 expression on circulating versus tissue endothelial cells in rectal cancer patients: implications for circulating endothelial and progenitor cells as biomarkers for antiangiogenic therapy. J Clin Oncol (2006) 2.58

Kinetics and viability of circulating endothelial cells as surrogate angiogenesis marker in an animal model of human lymphoma. Cancer Res (2001) 2.17

Detection of circulating endothelial cells: CD146-based magnetic separation enrichment or flow cytometric assay? J Clin Oncol (2007) 1.27

Articles by these authors

Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature (2010) 15.28

Molecular mechanisms and clinical applications of angiogenesis. Nature (2011) 13.36

AZD2171, a pan-VEGF receptor tyrosine kinase inhibitor, normalizes tumor vasculature and alleviates edema in glioblastoma patients. Cancer Cell (2007) 12.52

Direct evidence that the VEGF-specific antibody bevacizumab has antivascular effects in human rectal cancer. Nat Med (2004) 9.80

Stem-cell ageing modified by the cyclin-dependent kinase inhibitor p16INK4a. Nature (2006) 9.20

Kinetics of vascular normalization by VEGFR2 blockade governs brain tumor response to radiation: role of oxygenation, angiopoietin-1, and matrix metalloproteinases. Cancer Cell (2004) 8.78

Photodynamic therapy for cancer. Nat Rev Cancer (2003) 8.00

Deconstructing stem cell self-renewal: genetic insights into cell-cycle regulation. Nat Rev Genet (2008) 7.30

Live-animal tracking of individual haematopoietic stem/progenitor cells in their niche. Nature (2008) 6.61

In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment. Nature (2005) 6.60

Vascular normalization by vascular endothelial growth factor receptor 2 blockade induces a pressure gradient across the vasculature and improves drug penetration in tumors. Cancer Res (2004) 5.65

Normalization of the vasculature for treatment of cancer and other diseases. Physiol Rev (2011) 5.64

Principles and mechanisms of vessel normalization for cancer and other angiogenic diseases. Nat Rev Drug Discov (2011) 5.59

Stem cell engraftment at the endosteal niche is specified by the calcium-sensing receptor. Nature (2005) 5.54

Dynamic imaging of collagen and its modulation in tumors in vivo using second-harmonic generation. Nat Med (2003) 5.50

Bone progenitor dysfunction induces myelodysplasia and secondary leukaemia. Nature (2010) 5.43

Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging. Nat Med (2009) 5.41

Lymphatic metastasis in the absence of functional intratumor lymphatics. Science (2002) 5.32

Abnormalities in pericytes on blood vessels and endothelial sprouts in tumors. Am J Pathol (2002) 5.04

Normalization of tumour blood vessels improves the delivery of nanomedicines in a size-dependent manner. Nat Nanotechnol (2012) 4.84

Endogenous bone marrow MSCs are dynamic, fate-restricted participants in bone maintenance and regeneration. Cell Stem Cell (2012) 4.67

A microenvironment-induced myeloproliferative syndrome caused by retinoic acid receptor gamma deficiency. Cell (2007) 4.59

Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study. J Clin Oncol (2009) 4.54

Tissue engineering: creation of long-lasting blood vessels. Nature (2004) 4.50

Osteopontin is a hematopoietic stem cell niche component that negatively regulates stem cell pool size. J Exp Med (2005) 4.17

Restoration of tubular epithelial cells during repair of the postischemic kidney occurs independently of bone marrow-derived stem cells. J Clin Invest (2005) 4.10

Therapeutic targeting of a stem cell niche. Nat Biotechnol (2007) 3.88

Hearing improvement after bevacizumab in patients with neurofibromatosis type 2. N Engl J Med (2009) 3.85

MicroRNA-mediated control of cell fate in megakaryocyte-erythrocyte progenitors. Dev Cell (2008) 3.84

Surrogate markers for antiangiogenic therapy and dose-limiting toxicities for bevacizumab with radiation and chemotherapy: continued experience of a phase I trial in rectal cancer patients. J Clin Oncol (2005) 3.83

Phase III randomized trial comparing the efficacy of cediranib as monotherapy, and in combination with lomustine, versus lomustine alone in patients with recurrent glioblastoma. J Clin Oncol (2013) 3.81

Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood. Cell (2005) 3.77

The hematopoietic stem cell in its place. Nat Immunol (2006) 3.70

Tumor microvasculature and microenvironment: targets for anti-angiogenesis and normalization. Microvasc Res (2007) 3.64

Wnt signaling in the niche enforces hematopoietic stem cell quiescence and is necessary to preserve self-renewal in vivo. Cell Stem Cell (2008) 3.62

Pathology: cancer cells compress intratumour vessels. Nature (2004) 3.60

The Lkb1 metabolic sensor maintains haematopoietic stem cell survival. Nature (2010) 3.55

Phase II study of cediranib, an oral pan-vascular endothelial growth factor receptor tyrosine kinase inhibitor, in patients with recurrent glioblastoma. J Clin Oncol (2010) 3.43

Tumour biology: herceptin acts as an anti-angiogenic cocktail. Nature (2002) 3.40

Efficacy, safety, and biomarkers of neoadjuvant bevacizumab, radiation therapy, and fluorouracil in rectal cancer: a multidisciplinary phase II study. J Clin Oncol (2009) 3.39

Multistage nanoparticle delivery system for deep penetration into tumor tissue. Proc Natl Acad Sci U S A (2011) 3.34

BIM expression in treatment-naive cancers predicts responsiveness to kinase inhibitors. Cancer Discov (2011) 3.34

Rituximab does not improve clinical outcome in a randomized phase 3 trial of CHOP with or without rituximab in patients with HIV-associated non-Hodgkin lymphoma: AIDS-Malignancies Consortium Trial 010. Blood (2005) 3.26

Diffusion and convection in collagen gels: implications for transport in the tumor interstitium. Biophys J (2002) 3.25

Quantum dots spectrally distinguish multiple species within the tumor milieu in vivo. Nat Med (2005) 3.19

Bone marrow-derived mesenchymal stem cells facilitate engineering of long-lasting functional vasculature. Blood (2008) 3.13

The role of nitric oxide in tumour progression. Nat Rev Cancer (2006) 3.08

Edema control by cediranib, a vascular endothelial growth factor receptor-targeted kinase inhibitor, prolongs survival despite persistent brain tumor growth in mice. J Clin Oncol (2009) 3.02

A "vascular normalization index" as potential mechanistic biomarker to predict survival after a single dose of cediranib in recurrent glioblastoma patients. Cancer Res (2009) 3.00

Challenges and key considerations of the enhanced permeability and retention effect for nanomedicine drug delivery in oncology. Cancer Res (2013) 2.98

Xist RNA is a potent suppressor of hematologic cancer in mice. Cell (2013) 2.93

Limiting factors in murine hematopoietic stem cell assays. Cell Stem Cell (2007) 2.89

The leukemic stem cell niche: current concepts and therapeutic opportunities. Blood (2009) 2.85

AKT/FOXO signaling enforces reversible differentiation blockade in myeloid leukemias. Cell (2011) 2.77

CD133 expression defines a tumor initiating cell population in primary human ovarian cancer. Stem Cells (2009) 2.76

Response criteria for glioma. Nat Clin Pract Oncol (2008) 2.69

Engineering vascularized tissue. Nat Biotechnol (2005) 2.69

Tumor microenvironment abnormalities: causes, consequences, and strategies to normalize. J Cell Biochem (2007) 2.67

Causes, consequences, and remedies for growth-induced solid stress in murine and human tumors. Proc Natl Acad Sci U S A (2012) 2.66

Notch1 activation increases hematopoietic stem cell self-renewal in vivo and favors lymphoid over myeloid lineage outcome. Blood (2002) 2.66

In vivo self-renewing divisions of haematopoietic stem cells are increased in the absence of the early G1-phase inhibitor, p18INK4C. Nat Cell Biol (2004) 2.65

Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat Biotechnol (2007) 2.65

Targeting placental growth factor/neuropilin 1 pathway inhibits growth and spread of medulloblastoma. Cell (2013) 2.64

Differential in vivo potential of endothelial progenitor cells from human umbilical cord blood and adult peripheral blood to form functional long-lasting vessels. Blood (2007) 2.64

Malignant cells facilitate lung metastasis by bringing their own soil. Proc Natl Acad Sci U S A (2010) 2.62

Differential CD146 expression on circulating versus tissue endothelial cells in rectal cancer patients: implications for circulating endothelial and progenitor cells as biomarkers for antiangiogenic therapy. J Clin Oncol (2006) 2.58

Tumor microvasculature and microenvironment: novel insights through intravital imaging in pre-clinical models. Microcirculation (2010) 2.53

MicroRNA miR-125a controls hematopoietic stem cell number. Proc Natl Acad Sci U S A (2010) 2.53

Vascular normalizing doses of antiangiogenic treatment reprogram the immunosuppressive tumor microenvironment and enhance immunotherapy. Proc Natl Acad Sci U S A (2012) 2.50

Differential regulation of myeloid leukemias by the bone marrow microenvironment. Nat Med (2013) 2.48

Haploinsufficiency of GATA-2 perturbs adult hematopoietic stem-cell homeostasis. Blood (2005) 2.47

Prospects for stem cell-based therapy. Cell (2008) 2.45

In vivo imaging of Treg cells providing immune privilege to the haematopoietic stem-cell niche. Nature (2011) 2.44

Imaging steps of lymphatic metastasis reveals that vascular endothelial growth factor-C increases metastasis by increasing delivery of cancer cells to lymph nodes: therapeutic implications. Cancer Res (2006) 2.42

Tumor stromal-derived factor-1 recruits vascular progenitors to mitotic neovasculature, where microenvironment influences their differentiated phenotypes. Cancer Res (2006) 2.42

Increased survival of glioblastoma patients who respond to antiangiogenic therapy with elevated blood perfusion. Cancer Res (2011) 2.40

CXCL12 (SDF1alpha)-CXCR4/CXCR7 pathway inhibition: an emerging sensitizer for anticancer therapies? Clin Cancer Res (2011) 2.39

Degradation of fibrillar collagen in a human melanoma xenograft improves the efficacy of an oncolytic herpes simplex virus vector. Cancer Res (2006) 2.35

BM mesenchymal stromal cell-derived exosomes facilitate multiple myeloma progression. J Clin Invest (2013) 2.34

Angiotensin inhibition enhances drug delivery and potentiates chemotherapy by decompressing tumour blood vessels. Nat Commun (2013) 2.31

Acid production in glycolysis-impaired tumors provides new insights into tumor metabolism. Clin Cancer Res (2002) 2.29

Losartan inhibits collagen I synthesis and improves the distribution and efficacy of nanotherapeutics in tumors. Proc Natl Acad Sci U S A (2011) 2.27

Cancers predispose neutrophils to release extracellular DNA traps that contribute to cancer-associated thrombosis. Proc Natl Acad Sci U S A (2012) 2.23

The candidate tumour suppressor protein ING4 regulates brain tumour growth and angiogenesis. Nature (2004) 2.22