PubRank

Joseph D Puglisi

Author PubWeight™ 85.56‹?›

Top papers

Rank Title Journal Year PubWeight™‹?›
1 tRNA dynamics on the ribosome during translation. Proc Natl Acad Sci U S A 2004 5.38
2 tRNA selection and kinetic proofreading in translation. Nat Struct Mol Biol 2004 4.54
3 An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. Biophys J 2007 4.49
4 Real-time tRNA transit on single translating ribosomes at codon resolution. Nature 2010 3.87
5 Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor. Nature 2010 3.19
6 Structure of HCV IRES domain II determined by NMR. Nat Struct Biol 2003 2.93
7 The pathway of HCV IRES-mediated translation initiation. Cell 2004 2.72
8 Site-specific labeling of the ribosome for single-molecule spectroscopy. Nucleic Acids Res 2005 1.97
9 Heterogeneous pathways and timing of factor departure during translation initiation. Nature 2012 1.91
10 Irreversible chemical steps control intersubunit dynamics during translation. Proc Natl Acad Sci U S A 2008 1.88
11 Translation at the single-molecule level. Annu Rev Biochem 2008 1.87
12 Fluctuations of transfer RNAs between classical and hybrid states. Biophys J 2007 1.82
13 The role of fluctuations in tRNA selection by the ribosome. Proc Natl Acad Sci U S A 2007 1.69
14 Solution structure and proposed domain domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase. Protein Sci 2007 1.68
15 GTP hydrolysis by IF2 guides progression of the ribosome into elongation. Mol Cell 2009 1.59
16 Following the intersubunit conformation of the ribosome during translation in real time. Nat Struct Mol Biol 2010 1.56
17 Peptide bond formation destabilizes Shine-Dalgarno interaction on the ribosome. Nature 2007 1.53
18 Ribosomal proteins mediate the hepatitis C virus IRES-HeLa 40S interaction. RNA 2002 1.48
19 NMR study of 100 kDa HCV IRES RNA using segmental isotope labeling. J Am Chem Soc 2002 1.47
20 Coordinated conformational and compositional dynamics drive ribosome translocation. Nat Struct Mol Biol 2013 1.47
21 Quantitative polysome analysis identifies limitations in bacterial cell-free protein synthesis. Biotechnol Bioeng 2005 1.44
22 Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides. RNA 2004 1.42
23 Solution structure and backbone dynamics of the holo form of the frenolicin acyl carrier protein. Biochemistry 2003 1.34
24 Purification and characterization of transcribed RNAs using gel filtration chromatography. Nat Protoc 2007 1.30
25 Rapid purification of RNAs using fast performance liquid chromatography (FPLC). RNA 2006 1.25
26 Comparison of X-ray crystal structure of the 30S subunit-antibiotic complex with NMR structure of decoding site oligonucleotide-paromomycin complex. Structure 2003 1.25
27 Molecular framework for the activation of RNA-dependent protein kinase. J Biol Chem 2007 1.21
28 Uncoupling of RNA binding and PKR kinase activation by viral inhibitor RNAs. J Mol Biol 2006 1.18
29 Single ribosome dynamics and the mechanism of translation. Annu Rev Biophys 2010 1.18
30 Unraveling the dynamics of ribosome translocation. Curr Opin Struct Biol 2012 1.15
31 Specific recognition of HIV TAR RNA by the dsRNA binding domains (dsRBD1-dsRBD2) of PKR. J Mol Biol 2006 1.13
32 High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence. Proc Natl Acad Sci U S A 2013 1.11
33 Thiostrepton inhibition of tRNA delivery to the ribosome. RNA 2007 1.09
34 Dynamics of the translational machinery. Curr Opin Struct Biol 2011 1.08
35 Solution mapping of T cell receptor docking footprints on peptide-MHC. Proc Natl Acad Sci U S A 2007 1.06
36 Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket. J Am Chem Soc 2012 1.06
37 The anti-hepatitis C agent nitazoxanide induces phosphorylation of eukaryotic initiation factor 2alpha via protein kinase activated by double-stranded RNA activation. Gastroenterology 2009 1.05
38 Single-molecule analysis of translational dynamics. Cold Spring Harb Perspect Biol 2012 1.04
39 Nonfluorescent quenchers to correlate single-molecule conformational and compositional dynamics. J Am Chem Soc 2012 1.04
40 Translational insensitivity to potent activation of PKR by HCV IRES RNA. Antiviral Res 2009 1.02
41 Structure determination of large biological RNAs. Methods Enzymol 2005 0.98
42 Viral dsRNA inhibitors prevent self-association and autophosphorylation of PKR. J Mol Biol 2007 0.98
43 Single-molecule imaging of full protein synthesis by immobilized ribosomes. Nucleic Acids Res 2008 0.95
44 Thermodynamic stability and structural features of the J4/5 loop in a Pneumocystis carinii group I intron. Biochemistry 2003 0.93
45 The impact of aminoglycosides on the dynamics of translation elongation. Cell Rep 2013 0.92
46 Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor. Proc Natl Acad Sci U S A 2013 0.92
47 Design of a cyclic peptide that targets a viral RNA. J Am Chem Soc 2003 0.88
48 Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations. Nucleic Acids Res 2010 0.87
49 Biophysical and biochemical investigations of dsRNA-activated kinase PKR. Methods Enzymol 2007 0.86
50 Sequence-specific recognition of the major groove of RNA by deoxystreptamine. Biochemistry 2002 0.86
51 Dynamic recognition of the mRNA cap by Saccharomyces cerevisiae eIF4E. Structure 2013 0.83
52 Probing the conformation of human tRNA(3)(Lys) in solution by NMR. FEBS Lett 2007 0.82
53 Initiation factor 2, tRNA, and 50S subunits cooperatively stabilize mRNAs on the ribosome during initiation. Proc Natl Acad Sci U S A 2012 0.80
54 RNA purification by preparative polyacrylamide gel electrophoresis. Methods Enzymol 2013 0.80
55 Secondary structure of the HIV reverse transcription initiation complex by NMR. J Mol Biol 2011 0.79
56 Analysis of RNA by analytical polyacrylamide gel electrophoresis. Methods Enzymol 2013 0.76
57 Structural biology: the dance of domains. Nature 2007 0.75
58 Nucleic acids continue to surprise. Curr Opin Struct Biol 2010 0.75
59 Nucleic acids and their protein partners. Curr Opin Struct Biol 2008 0.75
60 Digging deep into nucleic acid structure and nucleic acid protein recognition. Curr Opin Struct Biol 2012 0.75