Published in Biophys J on October 05, 2007
A practical guide to single-molecule FRET. Nat Methods (2008) 8.62
Real-time tRNA transit on single translating ribosomes at codon resolution. Nature (2010) 3.87
Structure and immune recognition of trimeric pre-fusion HIV-1 Env. Nature (2014) 3.50
Cyanine fluorophore derivatives with enhanced photostability. Nat Methods (2011) 2.85
The membrane skeleton controls diffusion dynamics and signaling through the B cell receptor. Immunity (2010) 2.75
Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging. Annu Rev Phys Chem (2012) 2.19
VASP is a processive actin polymerase that requires monomeric actin for barbed end association. J Cell Biol (2010) 2.13
Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy. Proc Natl Acad Sci U S A (2009) 2.12
Mitigating unwanted photophysical processes for improved single-molecule fluorescence imaging. Biophys J (2009) 2.05
Characterizing the initial encounter complex in cadherin adhesion. Structure (2009) 1.90
Cell adhesion. The minimal cadherin-catenin complex binds to actin filaments under force. Science (2014) 1.89
Irreversible chemical steps control intersubunit dynamics during translation. Proc Natl Acad Sci U S A (2008) 1.88
Resolving cadherin interactions and binding cooperativity at the single-molecule level. Proc Natl Acad Sci U S A (2008) 1.81
Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT. Nat Methods (2014) 1.70
Histone H4 tail mediates allosteric regulation of nucleosome remodelling by linker DNA. Nature (2014) 1.59
GTP hydrolysis by IF2 guides progression of the ribosome into elongation. Mol Cell (2009) 1.59
Following the intersubunit conformation of the ribosome during translation in real time. Nat Struct Mol Biol (2010) 1.56
Synthesis and applications of RNAs with position-selective labelling and mosaic composition. Nature (2015) 1.53
Formation of salt bridges mediates internal dimerization of myosin VI medial tail domain. Structure (2010) 1.53
Resolution doubling in 3D-STORM imaging through improved buffers. PLoS One (2013) 1.44
Defining the limits of single-molecule FRET resolution in TIRF microscopy. Biophys J (2010) 1.43
Myosin VI dimerization triggers an unfolding of a three-helix bundle in order to extend its reach. Mol Cell (2009) 1.42
Correlated conformational events in EF-G and the ribosome regulate translocation. Nat Struct Mol Biol (2010) 1.39
Single-molecule-based super-resolution images in the presence of multiple fluorophores. Nano Lett (2011) 1.35
Ultra-stable organic fluorophores for single-molecule research. Chem Soc Rev (2014) 1.31
Acidification of the oxygen scavenging system in single-molecule fluorescence studies: in situ sensing with a ratiometric dual-emission probe. Anal Chem (2010) 1.29
Single-molecule analysis of a molecular disassemblase reveals the mechanism of Hsc70-driven clathrin uncoating. Nat Struct Mol Biol (2011) 1.26
Analysis of complex single-molecule FRET time trajectories. Methods Enzymol (2010) 1.23
A bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation. Science (2012) 1.23
Single molecule measurement of the "speed limit" of DNA polymerase. Proc Natl Acad Sci U S A (2009) 1.21
Condensed mitotic chromosome structure at nanometer resolution using PALM and EGFP- histones. PLoS One (2010) 1.21
Enzymatic oxygen scavenging for photostability without pH drop in single-molecule experiments. ACS Nano (2012) 1.20
Biological mechanisms, one molecule at a time. Genes Dev (2011) 1.19
High-resolution structure of the Escherichia coli ribosome. Nat Struct Mol Biol (2015) 1.14
DCDHF fluorophores for single-molecule imaging in cells. Chemphyschem (2009) 1.14
Dynamic pathways of -1 translational frameshifting. Nature (2014) 1.12
High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence. Proc Natl Acad Sci U S A (2013) 1.11
Single-molecule three-color FRET with both negligible spectral overlap and long observation time. PLoS One (2010) 1.10
Characterization of photoactivated singlet oxygen damage in single-molecule optical trap experiments. Biophys J (2009) 1.10
Counting bungarotoxin binding sites of nicotinic acetylcholine receptors in mammalian cells with high signal/noise ratios. Biophys J (2010) 1.10
A single-molecule dissection of ligand binding to a protein with intrinsic dynamics. Nat Chem Biol (2013) 1.08
A mobile kinesin-head intermediate during the ATP-waiting state. Proc Natl Acad Sci U S A (2009) 1.05
Chromophore formation in DsRed occurs by a branched pathway. J Am Chem Soc (2010) 1.03
The bright future of single-molecule fluorescence imaging. Curr Opin Chem Biol (2014) 1.03
Gel-assisted formation of giant unilamellar vesicles. Biophys J (2013) 1.02
Multicolor super-resolution DNA imaging for genetic analysis. Nano Lett (2012) 1.00
The dynamics of SecM-induced translational stalling. Cell Rep (2014) 0.97
Caught in the act: the lifetime of synaptic intermediates during the search for homology on DNA. Nucleic Acids Res (2009) 0.96
The changing point-spread function: single-molecule-based super-resolution imaging. Histochem Cell Biol (2014) 0.96
Signal recognition particle-ribosome binding is sensitive to nascent chain length. J Biol Chem (2014) 0.95
Accelerated single photon emission from dye molecule-driven nanoantennas assembled on DNA. Nat Commun (2012) 0.95
N(6)-methyladenosine in mRNA disrupts tRNA selection and translation-elongation dynamics. Nat Struct Mol Biol (2016) 0.95
Single-molecule imaging of an in vitro-evolved RNA aptamer reveals homogeneous ligand binding kinetics. J Am Chem Soc (2009) 0.94
The contribution of reactive oxygen species to the photobleaching of organic fluorophores. Photochem Photobiol (2013) 0.94
Single molecule fluorescence approaches shed light on intracellular RNAs. Chem Rev (2014) 0.94
Entropic origin of Mg2+-facilitated RNA folding. Proc Natl Acad Sci U S A (2012) 0.93
Prestin in HEK cells is an obligate tetramer. J Neurophysiol (2011) 0.93
Single-cell, real-time detection of oxidative stress induced in Escherichia coli by the antimicrobial peptide CM15. Proc Natl Acad Sci U S A (2015) 0.92
Opportunities and challenges in single-molecule and single-particle fluorescence microscopy for mechanistic studies of chemical reactions. Nat Chem (2013) 0.92
Gold Nanoparticles for In Vitro Diagnostics. Chem Rev (2015) 0.92
The eukaryotic initiation factor eIF4H facilitates loop-binding, repetitive RNA unwinding by the eIF4A DEAD-box helicase. Nucleic Acids Res (2012) 0.92
The impact of aminoglycosides on the dynamics of translation elongation. Cell Rep (2013) 0.92
Regulation of microtubule minus-end dynamics by CAMSAPs and Patronin. Proc Natl Acad Sci U S A (2014) 0.92
Single-molecule studies of the lysine riboswitch reveal effector-dependent conformational dynamics of the aptamer domain. Biochemistry (2012) 0.91
Single-molecule motions enable direct visualization of biomolecular interactions in solution. Nat Methods (2014) 0.91
Chemiluminescent detection of enzymatically produced hydrogen sulfide: substrate hydrogen bonding influences selectivity for H2S over biological thiols. J Am Chem Soc (2013) 0.91
Oxygen depletion triggers switching between discrete speed modes of gonococcal type IV pili. Biophys J (2012) 0.90
Small-molecule photostabilizing agents are modifiers of lipid bilayer properties. Biophys J (2013) 0.90
The ribosome uses cooperative conformational changes to maximize and regulate the efficiency of translation. Proc Natl Acad Sci U S A (2014) 0.90
Quantifying elongation rhythm during full-length protein synthesis. J Am Chem Soc (2013) 0.89
Single-molecule imaging of the oligomer formation of the nonhexameric Escherichia coli UvrD helicase. Biophys J (2013) 0.89
Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation. Nat Struct Mol Biol (2016) 0.88
Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations. Nucleic Acids Res (2010) 0.87
Tyrosination of α-tubulin controls the initiation of processive dynein-dynactin motility. EMBO J (2016) 0.87
The AAA3 domain of cytoplasmic dynein acts as a switch to facilitate microtubule release. Nat Struct Mol Biol (2014) 0.87
Mg(2+) Shifts Ligand-Mediated Folding of a Riboswitch from Induced-Fit to Conformational Selection. J Am Chem Soc (2015) 0.86
Structural Analysis of Multi-Helical RNAs by NMR-SAXS/WAXS: Application to the U4/U6 di-snRNA. J Mol Biol (2015) 0.86
Thermodynamic origins of monovalent facilitated RNA folding. Biochemistry (2012) 0.85
Assembly and dynamics of the U4/U6 di-snRNP by single-molecule FRET. Nucleic Acids Res (2015) 0.85
A bird's eye view tracking slow nanometer-scale movements of single molecular nano-assemblies. Methods Enzymol (2010) 0.85
Bright, Red Single-Molecule Emitters: Synthesis and Properties of Environmentally Sensitive Dicyanomethylenedihydrofuran (DCDHF) Fluorophores with Bisaromatic Conjugation. Chem Mater (2009) 0.84
Automated system for single molecule fluorescence measurements of surface-immobilized biomolecules. J Vis Exp (2009) 0.84
Single-molecule fluorescence resonance energy transfer studies of the human telomerase RNA pseudoknot: temperature-/urea-dependent folding kinetics and thermodynamics. J Phys Chem B (2014) 0.84
Bidirectional helical motility of cytoplasmic dynein around microtubules. Elife (2014) 0.84
The spectroscopic basis of fluorescence triple correlation spectroscopy. J Phys Chem B (2012) 0.84
An improved optical tweezers assay for measuring the force generation of single kinesin molecules. Methods Mol Biol (2014) 0.83
Chemically related 4,5-linked aminoglycoside antibiotics drive subunit rotation in opposite directions. Nat Commun (2015) 0.83
Dynamic recognition of the mRNA cap by Saccharomyces cerevisiae eIF4E. Structure (2013) 0.83
A dynamic RNA loop in an IRES affects multiple steps of elongation factor-mediated translation initiation. Elife (2015) 0.83
Pulsed IR heating studies of single-molecule DNA duplex dissociation kinetics and thermodynamics. Biophys J (2014) 0.82
The transition in spliceosome assembly from complex E to complex A purges surplus U1 snRNPs from alternative splice sites. Nucleic Acids Res (2012) 0.82
Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems. Chembiochem (2013) 0.82
Fast single-molecule FRET spectroscopy: theory and experiment. Phys Chem Chem Phys (2014) 0.82
Single-molecule conformational dynamics of a biologically functional hydroxocobalamin riboswitch. J Am Chem Soc (2014) 0.82
The covalent trimethoprim chemical tag facilitates single molecule imaging with organic fluorophores. Biophys J (2014) 0.82
Single-molecule spectroscopy of LHCSR1 protein dynamics identifies two distinct states responsible for multi-timescale photosynthetic photoprotection. Nat Chem (2017) 0.81
Widespread nuclease contamination in commonly used oxygen-scavenging systems. Nat Methods (2015) 0.81
Processive cytoskeletal motors studied with single-molecule fluorescence techniques. FEBS Lett (2014) 0.81
Kinetic fingerprinting to identify and count single nucleic acids. Nat Biotechnol (2015) 0.81
Direct observation of tropomyosin binding to actin filaments. Cytoskeleton (Hoboken) (2015) 0.81
Fluorescence spectroscopy of single biomolecules. Science (1999) 10.67
DITHIOTHREITOL, A NEW PROTECTIVE REAGENT FOR SH GROUPS. Biochemistry (1964) 10.04
Nonblinking and long-lasting single-molecule fluorescence imaging. Nat Methods (2006) 6.25
Fading of immunofluorescence during microscopy: a study of the phenomenon and its remedy. J Immunol Methods (1982) 5.95
Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science (1982) 5.92
Force measurements by micromanipulation of a single actin filament by glass needles. Nature (1988) 5.62
Carbocyanine dyes as efficient reversible single-molecule optical switch. J Am Chem Soc (2005) 5.56
tRNA dynamics on the ribosome during translation. Proc Natl Acad Sci U S A (2004) 5.38
Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase. Nature (2002) 5.13
Mechanochemical coupling in actomyosin energy transduction studied by in vitro movement assay. J Mol Biol (1990) 4.78
tRNA selection and kinetic proofreading in translation. Nat Struct Mol Biol (2004) 4.54
Identification of two distinct hybrid state intermediates on the ribosome. Mol Cell (2007) 4.00
Real-time observation of RecA filament dynamics with single monomer resolution. Cell (2006) 3.44
Single-molecule measurement of protein folding kinetics. Science (2003) 3.08
Rigidity of microtubules is increased by stabilizing agents. J Cell Biol (1995) 2.93
Fluctuating enzymes: lessons from single-molecule studies. Acc Chem Res (2005) 2.33
Single-molecule folding. Curr Opin Struct Biol (2003) 2.28
Single-molecule enzymology. J Biol Chem (1999) 2.10
Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy. J Phys Chem A (2007) 1.99
Biochemistry without oxygen. Anal Biochem (1987) 1.77
A comparison between the sulfhydryl reductants tris(2-carboxyethyl)phosphine and dithiothreitol for use in protein biochemistry. Anal Biochem (1999) 1.76
Single-molecule fluorescence spectroscopy of protein folding. Chemphyschem (2005) 1.70
A survey of single-molecule techniques in chemical biology. ACS Chem Biol (2007) 1.68
Reactive species formed on proteins exposed to singlet oxygen. Photochem Photobiol Sci (2003) 1.65
The use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments. Anal Biochem (2000) 1.61
Combining optical trapping and single-molecule fluorescence spectroscopy: enhanced photobleaching of fluorophores. J Phys Chem B (2004) 1.55
Spectroscopic study and evaluation of red-absorbing fluorescent dyes. Bioconjug Chem (2003) 1.48
Analysis of photobleaching in single-molecule multicolor excitation and Förster resonance energy transfer measurements. J Phys Chem A (2006) 1.42
Comparison of anti-fading agents used in fluorescence microscopy: image analysis and laser confocal microscopy study. J Histochem Cytochem (1993) 1.37
Single-molecule analysis of RNA polymerase transcription. Annu Rev Biophys Biomol Struct (2006) 1.37
Purification and properties of protocatechuate 3,4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry. J Biol Chem (1981) 1.34
Singlet oxygen induced DNA damage. Mutat Res (1992) 1.29
Photodestruction intermediates probed by an adjacent reporter molecule. Phys Rev Lett (2003) 1.27
Spectral identification of specific photophysics of cy5 by means of ensemble and single molecule measurements. J Phys Chem A (2006) 1.26
Analysis of antifading reagents for fluorescence microscopy. Cytometry (1995) 1.13
Measuring the folding transition time of single RNA molecules. Biophys J (2007) 1.09
Studies on ascorbic acid. I. Factors influencing the ascorbate-mediated inhibition of catalase. Biochemistry (1967) 1.06
Studies on ascorbic acid. II. Physical changes in catalase following incubation with ascorbate or ascorbate and copper (II). Biochemistry (1967) 1.01
Chemical reduction of disulfides. Methods Enzymol (1987) 1.00
Identification of an antioxidant, ethyl protocatechuate, in peanut seed testa. J Agric Food Chem (2003) 0.97
Direct observation of delayed fluorescence from a remarkable back-isomerization in Cy5. J Am Chem Soc (2005) 0.96
Antifading agents for confocal fluorescence microscopy. Methods Enzymol (1999) 0.96
Blinking fluorophores: what do they tell us about protein dynamics? Biochem Soc Trans (2006) 0.92
Biophysical analyses of designed and selected mutants of protocatechuate 3,4-dioxygenase1. Annu Rev Microbiol (2004) 0.87
Hemoglobin-oxygen equilibrium curves measured during enzymatic oxygen consumption. Anal Biochem (1998) 0.86
Singlet oxygen and dye-triplet-state quenching in solid-state dye lasers consisting of pyrromethene 567-doped poly(methyl methacrylate). Appl Opt (1999) 0.84
tRNA dynamics on the ribosome during translation. Proc Natl Acad Sci U S A (2004) 5.38
tRNA selection and kinetic proofreading in translation. Nat Struct Mol Biol (2004) 4.54
Real-time tRNA transit on single translating ribosomes at codon resolution. Nature (2010) 3.87
Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor. Nature (2010) 3.19
Structure of HCV IRES domain II determined by NMR. Nat Struct Biol (2003) 2.93
The pathway of HCV IRES-mediated translation initiation. Cell (2004) 2.72
Site-specific labeling of the ribosome for single-molecule spectroscopy. Nucleic Acids Res (2005) 1.97
Heterogeneous pathways and timing of factor departure during translation initiation. Nature (2012) 1.91
Irreversible chemical steps control intersubunit dynamics during translation. Proc Natl Acad Sci U S A (2008) 1.88
Translation at the single-molecule level. Annu Rev Biochem (2008) 1.87
Fluctuations of transfer RNAs between classical and hybrid states. Biophys J (2007) 1.82
The role of fluctuations in tRNA selection by the ribosome. Proc Natl Acad Sci U S A (2007) 1.69
Solution structure and proposed domain domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase. Protein Sci (2007) 1.68
GTP hydrolysis by IF2 guides progression of the ribosome into elongation. Mol Cell (2009) 1.59
Following the intersubunit conformation of the ribosome during translation in real time. Nat Struct Mol Biol (2010) 1.56
Peptide bond formation destabilizes Shine-Dalgarno interaction on the ribosome. Nature (2007) 1.53
Ribosomal proteins mediate the hepatitis C virus IRES-HeLa 40S interaction. RNA (2002) 1.48
NMR study of 100 kDa HCV IRES RNA using segmental isotope labeling. J Am Chem Soc (2002) 1.47
Coordinated conformational and compositional dynamics drive ribosome translocation. Nat Struct Mol Biol (2013) 1.47
Quantitative polysome analysis identifies limitations in bacterial cell-free protein synthesis. Biotechnol Bioeng (2005) 1.44
Large-scale preparation and purification of polyacrylamide-free RNA oligonucleotides. RNA (2004) 1.42
Solution structure and backbone dynamics of the holo form of the frenolicin acyl carrier protein. Biochemistry (2003) 1.34
Purification and characterization of transcribed RNAs using gel filtration chromatography. Nat Protoc (2007) 1.30
Rapid purification of RNAs using fast performance liquid chromatography (FPLC). RNA (2006) 1.25
Comparison of X-ray crystal structure of the 30S subunit-antibiotic complex with NMR structure of decoding site oligonucleotide-paromomycin complex. Structure (2003) 1.25
Molecular framework for the activation of RNA-dependent protein kinase. J Biol Chem (2007) 1.21
Uncoupling of RNA binding and PKR kinase activation by viral inhibitor RNAs. J Mol Biol (2006) 1.18
Single ribosome dynamics and the mechanism of translation. Annu Rev Biophys (2010) 1.18
Unraveling the dynamics of ribosome translocation. Curr Opin Struct Biol (2012) 1.15
Specific recognition of HIV TAR RNA by the dsRNA binding domains (dsRBD1-dsRBD2) of PKR. J Mol Biol (2006) 1.13
High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence. Proc Natl Acad Sci U S A (2013) 1.11
Thiostrepton inhibition of tRNA delivery to the ribosome. RNA (2007) 1.09
Dynamics of the translational machinery. Curr Opin Struct Biol (2011) 1.08
Solution mapping of T cell receptor docking footprints on peptide-MHC. Proc Natl Acad Sci U S A (2007) 1.06
Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket. J Am Chem Soc (2012) 1.06
The anti-hepatitis C agent nitazoxanide induces phosphorylation of eukaryotic initiation factor 2alpha via protein kinase activated by double-stranded RNA activation. Gastroenterology (2009) 1.05
Single-molecule analysis of translational dynamics. Cold Spring Harb Perspect Biol (2012) 1.04
Nonfluorescent quenchers to correlate single-molecule conformational and compositional dynamics. J Am Chem Soc (2012) 1.04
Translational insensitivity to potent activation of PKR by HCV IRES RNA. Antiviral Res (2009) 1.02
Structure determination of large biological RNAs. Methods Enzymol (2005) 0.98
Viral dsRNA inhibitors prevent self-association and autophosphorylation of PKR. J Mol Biol (2007) 0.98
Single-molecule imaging of full protein synthesis by immobilized ribosomes. Nucleic Acids Res (2008) 0.95
Thermodynamic stability and structural features of the J4/5 loop in a Pneumocystis carinii group I intron. Biochemistry (2003) 0.93
The impact of aminoglycosides on the dynamics of translation elongation. Cell Rep (2013) 0.92
Involvement of protein IF2 N domain in ribosomal subunit joining revealed from architecture and function of the full-length initiation factor. Proc Natl Acad Sci U S A (2013) 0.92
Design of a cyclic peptide that targets a viral RNA. J Am Chem Soc (2003) 0.88
Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations. Nucleic Acids Res (2010) 0.87
Biophysical and biochemical investigations of dsRNA-activated kinase PKR. Methods Enzymol (2007) 0.86
Sequence-specific recognition of the major groove of RNA by deoxystreptamine. Biochemistry (2002) 0.86
Dynamic recognition of the mRNA cap by Saccharomyces cerevisiae eIF4E. Structure (2013) 0.83
Probing the conformation of human tRNA(3)(Lys) in solution by NMR. FEBS Lett (2007) 0.82
Initiation factor 2, tRNA, and 50S subunits cooperatively stabilize mRNAs on the ribosome during initiation. Proc Natl Acad Sci U S A (2012) 0.80
RNA purification by preparative polyacrylamide gel electrophoresis. Methods Enzymol (2013) 0.80
Secondary structure of the HIV reverse transcription initiation complex by NMR. J Mol Biol (2011) 0.79
Templated conversion of a crown ether-containing macrobicycle into [2]rotaxanes. J Org Chem (2002) 0.76
Analysis of RNA by analytical polyacrylamide gel electrophoresis. Methods Enzymol (2013) 0.76
Structural biology: the dance of domains. Nature (2007) 0.75
Nucleic acids continue to surprise. Curr Opin Struct Biol (2010) 0.75
Digging deep into nucleic acid structure and nucleic acid protein recognition. Curr Opin Struct Biol (2012) 0.75
Nucleic acids and their protein partners. Curr Opin Struct Biol (2008) 0.75