High-resolution structure of a Na+/H+ antiporter dimer obtained by pulsed electron paramagnetic resonance distance measurements.

PubWeight™: 1.21‹?› | Rank: Top 10%

🔗 View Article (PMC 2072073)

Published in Biophys J on August 17, 2007

Authors

D Hilger1, Y Polyhach, E Padan, H Jung, G Jeschke

Author Affiliations

1: Ludwig-Maximilians-Universität München, Department Biologie I, D-80638 Munich, Germany.

Articles citing this

Toward the fourth dimension of membrane protein structure: insight into dynamics from spin-labeling EPR spectroscopy. Structure (2011) 1.68

Benchmarking membrane protein detergent stability for improving throughput of high-resolution X-ray structures. Structure (2011) 1.62

PELDOR spectroscopy distance fingerprinting of the octameric outer-membrane protein Wza from Escherichia coli. Angew Chem Int Ed Engl (2009) 1.10

Backbone structure of transmembrane domain IX of the Na+/proline transporter PutP of Escherichia coli. Biophys J (2009) 1.10

KTN (RCK) domains regulate K+ channels and transporters by controlling the dimer-hinge conformation. Structure (2009) 1.05

Structure-based functional study reveals multiple roles of transmembrane segment IX and loop VIII-IX in NhaA Na+/H+ antiporter of Escherichia coli at physiological pH. J Biol Chem (2008) 1.03

Algorithm for selection of optimized EPR distance restraints for de novo protein structure determination. J Struct Biol (2010) 1.02

Global structure of a three-way junction in a phi29 packaging RNA dimer determined using site-directed spin labeling. J Am Chem Soc (2012) 0.98

Determination of structural models of the complex between the cytoplasmic domain of erythrocyte band 3 and ankyrin-R repeats 13-24. J Biol Chem (2011) 0.96

The global analysis of DEER data. J Magn Reson (2012) 0.95

Structure and dynamics of the force-generating domain of myosin probed by multifrequency electron paramagnetic resonance. Biophys J (2008) 0.93

Structural modeling and electron paramagnetic resonance spectroscopy of the human Na+/H+ exchanger isoform 1, NHE1. J Biol Chem (2010) 0.92

Electron spin resonance shows common structural features for different classes of EcoRI-DNA complexes. Angew Chem Int Ed Engl (2008) 0.85

Lesion-induced DNA weak structural changes detected by pulsed EPR spectroscopy combined with site-directed spin labelling. Nucleic Acids Res (2009) 0.85

A comparative study of structures and structural transitions of secondary transporters with the LeuT fold. Eur Biophys J (2012) 0.82

Structural and functional importance of transmembrane domain 3 (TM3) in the aspartate:alanine antiporter AspT: topology and function of the residues of TM3 and oligomerization of AspT. J Bacteriol (2009) 0.81

Differential effects of mutations on the transport properties of the Na+/H+ antiporter NhaA from Escherichia coli. J Biol Chem (2013) 0.80

mtsslSuite: In silico spin labelling, trilateration and distance-constrained rigid body docking in PyMOL. Mol Phys (2013) 0.80

Simulating the dynamics and orientations of spin-labeled side chains in a protein-DNA complex. J Phys Chem B (2012) 0.79

Accurate Extraction of Nanometer Distances in Multimers by Pulse EPR. Chemistry (2016) 0.78

Measurement of rate constants for homodimer subunit exchange using double electron-electron resonance and paramagnetic relaxation enhancements. J Biomol NMR (2012) 0.78

High-resolution crystal structure of spin labelled (T21R1) azurin from Pseudomonas aeruginosa: a challenging structural benchmark for in silico spin labelling algorithms. BMC Struct Biol (2014) 0.78

Integrating mass spectrometry with MD simulations reveals the role of lipids in Na(+)/H(+) antiporters. Nat Commun (2017) 0.78

Computational study of the Na+/H + antiporter from Vibrio parahaemolyticus. J Mol Model (2010) 0.76

Relative Orientation of POTRA Domains from Cyanobacterial Omp85 Studied by Pulsed EPR Spectroscopy. Biophys J (2016) 0.75

Articles cited by this

SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling. Electrophoresis (1997) 57.27

HADDOCK: a protein-protein docking approach based on biochemical or biophysical information. J Am Chem Soc (2003) 11.65

Automated analysis of interatomic contacts in proteins. Bioinformatics (1999) 5.60

Dead-time free measurement of dipole-dipole interactions between electron spins. J Magn Reson (2000) 5.26

Distance measurements on spin-labelled biomacromolecules by pulsed electron paramagnetic resonance. Phys Chem Chem Phys (2007) 4.08

Transmembrane protein structure: spin labeling of bacteriorhodopsin mutants. Science (1990) 4.07

Reconstruction of the chemotaxis receptor-kinase assembly. Nat Struct Mol Biol (2006) 3.94

Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH. Nature (2005) 3.74

Determination of the distance between two spin labels attached to a macromolecule. Proc Natl Acad Sci U S A (1995) 3.44

The determination of pair distance distributions by pulsed ESR using Tikhonov regularization. J Magn Reson (2005) 3.26

Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli. J Biol Chem (1991) 2.26

Explicit treatment of spin labels in modeling of distance constraints from dipolar EPR and DEER. J Am Chem Soc (2005) 1.72

Direct conversion of EPR dipolar time evolution data to distance distributions. J Magn Reson (2002) 1.64

Calculation of electron paramagnetic resonance spectra from Brownian dynamics trajectories: application to nitroxide side chains in proteins. Biophys J (1996) 1.55

Three-dimensional structure of the ion-coupled transport protein NhaA. Nature (2000) 1.53

NhaA of Escherichia coli, as a model of a pH-regulated Na+/H+antiporter. Biochim Biophys Acta (2004) 1.51

Inter-helix distances in lysophospholipid micelle-bound alpha-synuclein from pulsed ESR measurements. J Am Chem Soc (2006) 1.44

Sensitivity enhancement in pulse EPR distance measurements. J Magn Reson (2004) 1.31

Projection structure of NhaA, a secondary transporter from Escherichia coli, at 4.0 A resolution. EMBO J (1999) 1.28

Assessing oligomerization of membrane proteins by four-pulse DEER: pH-dependent dimerization of NhaA Na+/H+ antiporter of E. coli. Biophys J (2005) 1.24

A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside. J Biol Chem (1999) 1.16

Unidirectional reconstitution and characterization of purified Na+/proline transporter of Escherichia coli. Biochemistry (1998) 1.15

Oligomerization of NhaA, the Na+/H+ antiporter of Escherichia coli in the membrane and its functional and structural consequences. Biochemistry (2001) 1.14

A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli. J Biol Chem (1998) 1.13

Multiconformation continuum electrostatics analysis of the NhaA Na+/H+ antiporter of Escherichia coli with functional implications. Proc Natl Acad Sci U S A (2006) 1.08

Calculation of rigid-body conformational changes using restraint-driven Cartesian transformations. Biophys J (2001) 0.95

Crucial steps in the structure determination of the Na+/H+ antiporter NhaA in its native conformation. J Mol Biol (2006) 0.95

Distance between a native cofactor and a spin label in the reaction centre of Rhodobacter sphaeroides by a two-frequency pulsed electron paramagnetic resonance method and molecular dynamics simulations. J Magn Reson (2006) 0.94

Proximity of cytoplasmic and periplasmic loops in NhaA Na+/H+ antiporter of Escherichia coli as determined by site-directed thiol cross-linking. Biochemistry (2002) 0.89

Articles by these authors

(truncated to the top 100)

Dead-time free measurement of dipole-dipole interactions between electron spins. J Magn Reson (2000) 5.26

The proton electrochemical gradient in Escherichia coli cells. Eur J Biochem (1976) 5.13

pH homeostasis in bacteria. Biochim Biophys Acta (1981) 4.72

Proton electrochemical gradient in Escherichia coli cells and its relation to active transport of lactose. Biochemistry (1979) 3.06

Characterization of a Na+/H+ antiporter gene of Escherichia coli. Proc Natl Acad Sci U S A (1987) 2.51

Escherichia coli intracellular pH, membrane potential, and cell growth. J Bacteriol (1984) 2.51

Occurrence of facultative anoxygenic photosynthesis among filamentous and unicellular cyanobacteria. J Bacteriol (1977) 2.51

Facultative anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. J Bacteriol (1975) 2.40

Energy transduction in the photosynthetic membranes of the cyanobacterium (blue-green alga) P-lectonema boryanum. J Biol Chem (1978) 2.29

Overproduction and purification of a functional Na+/H+ antiporter coded by nhaA (ant) from Escherichia coli. J Biol Chem (1991) 2.26

The sodium/proton antiporter is part of the pH homeostasis mechanism in Escherichia coli. J Biol Chem (1982) 2.20

Induction of anaerobic, photoautotrophic growth in the cyanobacterium Oscillatoria limnetica. J Bacteriol (1978) 2.18

Cyanophages-viruses attacking blue-green algae. Bacteriol Rev (1973) 2.08

Isolation of "cyanophages" from freshwater ponds and their interaction with Plectonema boryanum. Virology (1967) 1.97

The role of T cells in anti-herpes simplex virus immunity. I. Induction of antigen-specific cytotoxic T lymphocytes. J Immunol (1977) 1.86

Cloning and characterization of a putative Ca2+/H+ antiporter gene from Escherichia coli upon functional complementation of Na+/H+ antiporter-deficient strains by the overexpressed gene. J Biol Chem (1993) 1.75

Intracellular pH and membrane potential as regulators in the prokaryotic cell. J Membr Biol (1987) 1.69

Transverse-momentum and pseudorapidity distributions of charged hadrons in pp collisions at square root of s = 7 TeV. Phys Rev Lett (2010) 1.67

Cloning, sequencing, and expression of the nhaB gene, encoding a Na+/H+ antiporter in Escherichia coli. J Biol Chem (1992) 1.65

Physiological role of nhaB, a specific Na+/H+ antiporter in Escherichia coli. J Biol Chem (1993) 1.65

A single locus in Escherichia coli governs growth in alkaline pH and on carbon sources whose transport is sodium dependent. FEBS Lett (1980) 1.60

Respiratory control and the proton electrochemical gradient in mitochondria. Eur J Biochem (1973) 1.58

Dead-time free measurement of dipole-dipole interactions between electron spins. 2000. J Magn Reson (2011) 1.58

Expression of a sodium proton antiporter (NhaA) in Escherichia coli is induced by Na+ and Li+ ions. J Biol Chem (1991) 1.57

Study of the mass and spin-parity of the Higgs boson candidate via its decays to Z boson pairs. Phys Rev Lett (2013) 1.56

An alkaline shift induces the heat shock response in Escherichia coli. J Bacteriol (1987) 1.55

Sequencing of the gene ant which affects the Na+/H+ antiporter activity in Escherichia coli. J Biol Chem (1988) 1.55

Use of site-directed fluorescence labeling to study proximity relationships in the lactose permease of Escherichia coli. Biochemistry (1993) 1.54

Sulfide inhibition of photosystem II in cyanobacteria (blue-green algae) and tobacco chloroplasts. Biochim Biophys Acta (1979) 1.48

Proton-sodium stoichiometry of NhaA, an electrogenic antiporter from Escherichia coli. J Biol Chem (1993) 1.47

Properties and purification of an active biotinylated lactose permease from Escherichia coli. Proc Natl Acad Sci U S A (1993) 1.43

Histidine-226 is part of the pH sensor of NhaA, a Na+/H+ antiporter in Escherichia coli. Proc Natl Acad Sci U S A (1993) 1.42

Regulation of ntcA expression and nitrite uptake in the marine Synechococcus sp. strain WH 7803. J Bacteriol (1998) 1.42

Delta pH and membrane potential in bacterial chromatophores. FEBS Lett (1974) 1.41

Quantum yields for oxygenic and anoxygenic photosynthesis in the cyanobacterium Oscillatoria limnetica. Proc Natl Acad Sci U S A (1977) 1.40

Late complications after a combined pre and postoperative (sandwich) radiotherapy for rectal cancer. Radiother Oncol (1999) 1.40

Distance measurements in the borderline region of applicability of CW EPR and DEER: a model study on a homologous series of spin-labelled peptides. J Magn Reson (2007) 1.37

NhaR, a protein homologous to a family of bacterial regulatory proteins (LysR), regulates nhaA, the sodium proton antiporter gene in Escherichia coli. J Biol Chem (1992) 1.36

Induction of SOS functions by alkaline intracellular pH in Escherichia coli. J Bacteriol (1986) 1.36

Light effect on the internal osmotic pressure of the blue-green algae Plectonema boryanum and Phormidium luridum. Arch Mikrobiol (1972) 1.35

Active transport of glucose and alpha-methylglucoside in the cyanobacterium Plectonema boryanum. J Biol Chem (1978) 1.34

Effect of diethylpyrocarbonate on lactose/proton symport in Escherichia coli membrane vesicles. Proc Natl Acad Sci U S A (1979) 1.34

The reproductive cycle of cyanophage LPP1-G in Plectonema boryanum and its dependence on photosynthetic and respiratory systems. Virology (1970) 1.32

The lactose permease meets Frankenstein. J Exp Biol (1994) 1.32

Kinetic properties of NhaB, a Na+/H+ antiporter from Escherichia coli. J Biol Chem (1994) 1.29

Projection structure of NhaA, a secondary transporter from Escherichia coli, at 4.0 A resolution. EMBO J (1999) 1.28

Intracellular pH regulation in bacterial cells. Methods Enzymol (1986) 1.27

Osmosensor and osmoregulator properties of the betaine carrier BetP from Corynebacterium glutamicum in proteoliposomes. J Biol Chem (2000) 1.27

Plasma levels of praziquantel decrease when dexamethasone is given simultaneously. Neurology (1987) 1.25

Measurement of the electron charge asymmetry in inclusive W production in pp collisions at sqrt[s]=7  TeV. Phys Rev Lett (2012) 1.23

Requirement for a membrane potential for cellulose synthesis in intact cells of Acetobacter xylinum. Proc Natl Acad Sci U S A (1982) 1.22

Search for new physics in the multijet and missing transverse momentum final state in proton-proton collisions at √s = 7 TeV. Phys Rev Lett (2012) 1.19

Azimuthal anisotropy of charged particles at high transverse momenta in Pb-Pb collisions at sqrt[s(NN)] = 2.76  TeV. Phys Rev Lett (2012) 1.19

Purification and reconstitution of an osmosensor: transporter ProP of Escherichia coli senses and responds to osmotic shifts. Biochemistry (1999) 1.18

Sulfur metabolism in Beggiatoa alba. J Bacteriol (1987) 1.18

Hydrogen metabolism in the facultative anoxygenic cyanobacteria (blue-green algae) Oscillatoria limnetica and Aphanothece halophytica. Arch Microbiol (1978) 1.18

Na+-induced transcription of nhaA, which encodes an Na+/H+ antiporter in Escherichia coli, is positively regulated by nhaR and affected by hns. J Bacteriol (1996) 1.17

A pH-dependent conformational change of NhaA Na(+)/H(+) antiporter of Escherichia coli involves loop VIII-IX, plays a role in the pH response of the protein, and is maintained by the pure protein in dodecyl maltoside. J Biol Chem (1999) 1.16

Endogenous dark respiration of the blue-green alga, Plectonema boryanum. J Bacteriol (1971) 1.14

Oligomerization of NhaA, the Na+/H+ antiporter of Escherichia coli in the membrane and its functional and structural consequences. Biochemistry (2001) 1.14

A point mutation (G338S) and its suppressor mutations affect both the pH response of the NhaA-Na+/H+ antiporter as well as the growth phenotype of Escherichia coli. J Biol Chem (1998) 1.13

Molecular cloning and sequencing of a gene from alkaliphilic Bacillus firmus OF4 that functionally complements an Escherichia coli strain carrying a deletion in the nhaA Na+/H+ antiporter gene. J Biol Chem (1991) 1.13

Na+ gradient-coupled porters of EScherichia coli share a common subunit. J Biol Chem (1982) 1.13

A conserved aspartate residue, Asp187, is important for Na+-dependent proline binding and transport by the Na+/proline transporter of Escherichia coli. Biochemistry (1998) 1.13

Characterization of site-directed mutants in the lac permease of Escherichia coli. 1. Replacement of histidine residues. Biochemistry (1989) 1.12

Effect of cyanophage infection on CO2 photoassimilation in Plectonema boryanum. J Virol (1968) 1.12

Purification and characterization of sulfide-quinone reductase, a novel enzyme driving anoxygenic photosynthesis in Oscillatoria limnetica. J Biol Chem (1994) 1.12

Elliptic flow of charged particles in Pb-Pb collisions at sqrt[S(NN)] = 2.76 TeV. Phys Rev Lett (2010) 1.12

Particle-yield modification in jetlike azimuthal dihadron correlations in Pb-Pb collisions at √s(NN)=2.76 TeV. Phys Rev Lett (2012) 1.11

Histidine 225, a residue of the NhaA-Na+/H+ antiporter of Escherichia coli is exposed and faces the cell exterior. J Biol Chem (1997) 1.10

Search for a W' or techni-ρ decaying into WZ in pp Collisions at sqrt[s] = 7  TeV. Phys Rev Lett (2012) 1.10

What's new with lactose permease. J Bioenerg Biomembr (1993) 1.10

Search for new physics with same-sign isolated dilepton events with jets and missing transverse energy. Phys Rev Lett (2012) 1.09

Dynamics of lactose permease of Escherichia coli determined by site-directed fluorescence labeling. Biochemistry (1994) 1.08

Search for supersymmetry at the LHC in events with jets and missing transverse energy. Phys Rev Lett (2011) 1.08

Transverse momentum distribution and nuclear modification factor of charged particles in p+Pb collisions at sqrt[s(NN)] = 5.02 TeV. Phys Rev Lett (2013) 1.08

Role of glycine residues in the structure and function of lactose permease, an Escherichia coli membrane transport protein. Biochemistry (1995) 1.07

Search for stopped Gluinos in pp collisions at square root s=7 TeV. Phys Rev Lett (2011) 1.07

Heterotrophic capacities of Plectonema boryanum. Arch Microbiol (1976) 1.07

Bioavailability of praziquantel increases with concomitant administration of food. Antimicrob Agents Chemother (2000) 1.06

Site-specific mutagenesis of histidine residues in the lac permease of Escherichia coli. Proc Natl Acad Sci U S A (1985) 1.06

Spin pair geometry revealed by high-field DEER in the presence of conformational distributions. J Magn Reson (2006) 1.06

Higher harmonic anisotropic flow measurements of charged particles in Pb-Pb collisions at sqrt(s(NN)) = 2.76  TeV. Phys Rev Lett (2011) 1.05

Measurement of the Υ1S, Υ2S, and Υ3S polarizations in pp collisions at sqrt[s] = 7 TeV. Phys Rev Lett (2013) 1.05

Oligonucleotide-directed site-specific mutagenesis of the lac permease of Escherichia coli. Methods Enzymol (1986) 1.04

Multi-institutional outcome study on the efficacy of closed-suction drainage of the scrotum in three-piece inflatable penile prosthesis surgery. Int J Impot Res (2005) 1.04

Changes of histological and proliferative indices in the Walker carcinoma with tumour size and distance from blood vessel. Eur J Cancer (1979) 1.03

Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli. Biochim Biophys Acta (2005) 1.02

Studies on the oxygen radical mechanism involved in the small intestinal reperfusion damage. Acta Physiol Scand (1985) 1.02

MH1, a second-site revertant of an Escherichia coli mutant lacking Na+/H+ antiporters (delta nhaA delta nhaB), regains Na+ resistance and a capacity to excrete Na+ in a delta microH(+)-independent fashion. J Biol Chem (1995) 1.02

Biosynthesis of the lactose permease in Escherichia coli minicells and effect of carrier amplification on cell physiology. J Biol Chem (1983) 1.01

Sulfide-quinone reductase from Rhodobacter capsulatus. Purification, cloning, and expression. J Biol Chem (1997) 1.01

Single-strand breaks induced in DNA by vacuum-ultraviolet radiation. Photochem Photobiol (1972) 1.00

Glucose levels are associated with cardiovascular disease and death in an international cohort of normal glycaemic and dysglycaemic men and women: the EpiDREAM cohort study. Eur J Prev Cardiol (2011) 1.00

Measurement of the polarization of W bosons with large transverse momenta in W + jets events at the LHC. Phys Rev Lett (2011) 0.99

Production of muons from heavy flavor decays at forward rapidity in pp and Pb-Pb collisions at sqrt[s(NN)]=2.76  TeV. Phys Rev Lett (2012) 0.98

A multicenter, randomized, controlled trial testing the effects of acupuncture on allergic rhinitis. Allergy (2012) 0.98

Measurement of the inclusive jet cross section in pp collisions at √s = 7 TeV. Phys Rev Lett (2011) 0.98

Search for neutral minimal supersymmetric standard model Higgs bosons decaying to tau pairs in pp collisions at √s=7 TeV. Phys Rev Lett (2011) 0.97

The monoclonal antibody 1F6 identifies a pH-dependent conformational change in the hydrophilic NH(2) terminus of NhaA Na(+)/H(+) antiporter of Escherichia coli. J Biol Chem (2000) 0.97