Published in J Biotechnol on August 03, 2007
Synthetic biology for the directed evolution of protein biocatalysts: navigating sequence space intelligently. Chem Soc Rev (2015) 1.74
Assessing directed evolution methods for the generation of biosynthetic enzymes with potential in drug biosynthesis. Future Med Chem (2011) 0.90
Directed evolution to re-adapt a co-evolved network within an enzyme. J Biotechnol (2011) 0.80
A metabolic prototype for eliminating tryptophan from the genetic code. Sci Rep (2013) 0.76
Droplet millifluidics for kinetic study of transketolase. Biomicrofluidics (2016) 0.75
Two strategies to engineer flexible loops for improved enzyme thermostability. Sci Rep (2017) 0.75
Structural Analysis of an Evolved Transketolase Reveals Divergent Binding Modes. Sci Rep (2016) 0.75
Genomic scale profiling of nutrient and trace elements in Arabidopsis thaliana. Nat Biotechnol (2003) 4.20
Expression patterns of a novel AtCHX gene family highlight potential roles in osmotic adjustment and K+ homeostasis in pollen development. Plant Physiol (2004) 1.58
Molecular pathogenicity of the oral opportunistic pathogen Actinobacillus actinomycetemcomitans. Annu Rev Microbiol (2003) 1.57
Integrating membrane transport with male gametophyte development and function through transcriptomics. Plant Physiol (2006) 1.45
Aggregatibacter (Actinobacillus) actinomycetemcomitans: a triple A* periodontopathogen? Periodontol 2000 (2010) 1.45
Protein-protein interactions between sucrose transporters of different affinities colocalized in the same enucleate sieve element. Plant Cell (2002) 1.45
Wake up! Peptidoglycan lysis and bacterial non-growth states. Trends Microbiol (2006) 1.42
Substrate specificity of the Arabidopsis thaliana sucrose transporter AtSUC2. J Biol Chem (2003) 1.41
Bromomaleimides: new reagents for the selective and reversible modification of cysteine. Chem Commun (Camb) (2009) 1.40
Tunable reagents for multi-functional bioconjugation: reversible or permanent chemical modification of proteins and peptides by control of maleimide hydrolysis. Chem Commun (Camb) (2011) 1.38
Arabidopsis sucrose transporter AtSUC9. High-affinity transport activity, intragenic control of expression, and early flowering mutant phenotype. Plant Physiol (2006) 1.37
In situ maleimide bridging of disulfides and a new approach to protein PEGylation. Bioconjug Chem (2011) 1.33
Functional analysis of LjSUT4, a vacuolar sucrose transporter from Lotus japonicus. Plant Mol Biol (2008) 1.28
Intra- and intermolecular interactions in sucrose transporters at the plasma membrane detected by the split-ubiquitin system and functional assays. Structure (2002) 1.24
Arabidopsis sucrose transporter AtSUC1 is important for pollen germination and sucrose-induced anthocyanin accumulation. Plant Physiol (2008) 1.24
Evolution of plant sucrose uptake transporters. Front Plant Sci (2012) 1.18
Impaired function of the tonoplast-localized sucrose transporter in rice, OsSUT2, limits the transport of vacuolar reserve sucrose and affects plant growth. Plant Physiol (2011) 1.16
AtPTR1, a plasma membrane peptide transporter expressed during seed germination and in vascular tissue of Arabidopsis. Plant J (2004) 1.16
Bromopyridazinedione-mediated protein and peptide bioconjugation. Chem Commun (Camb) (2011) 1.15
Participation of endomembrane cation/H+ exchanger AtCHX20 in osmoregulation of guard cells. Plant Physiol (2007) 1.12
Bromomaleimide-linked bioconjugates are cleavable in mammalian cells. Chembiochem (2011) 1.09
Phage display in the study of infectious diseases. Trends Microbiol (2006) 1.05
Analysis of the transport activity of barley sucrose transporter HvSUT1. Plant Cell Physiol (2005) 1.04
Actinobacillus actinomycetemcomitans. J Med Microbiol (2002) 1.03
Analysis of transport activity of Arabidopsis sugar alcohol permease homolog AtPLT5. J Biol Chem (2004) 1.02
Sugarcane ShSUT1: analysis of sucrose transport activity and inhibition by sucralose. Plant Cell Environ (2006) 1.02
Arabidopsis NPCC6/NaKR1 is a phloem mobile metal binding protein necessary for phloem function and root meristem maintenance. Plant Cell (2010) 1.00
Homogeneous antibody fragment conjugation by disulfide bridging introduces 'spinostics'. Sci Rep (2013) 0.99
Evolutionary analysis of the TPP-dependent enzyme family. J Mol Evol (2007) 0.99
Transport activity of rice sucrose transporters OsSUT1 and OsSUT5. Plant Cell Physiol (2009) 0.98
High-throughput measurement of protein stability in microtiter plates. Biotechnol Bioeng (2005) 0.98
Incorporation of paramagnetic, fluorescent and PET/SPECT contrast agents into liposomes for multimodal imaging. Biomaterials (2012) 0.96
Tumor-specific gene transfer with receptor-mediated nanocomplexes modified by polyethylene glycol shielding and endosomally cleavable lipid and peptide linkers. FASEB J (2010) 0.95
AtCHX13 is a plasma membrane K+ transporter. Plant Physiol (2008) 0.93
A receptor-targeted nanocomplex vector system optimized for respiratory gene transfer. Mol Ther (2008) 0.93
Integrin-targeted nanocomplexes for tumour specific delivery and therapy by systemic administration. Biomaterials (2010) 0.92
Multifunctional receptor-targeted nanocomplexes for the delivery of therapeutic nucleic acids to the brain. Biomaterials (2013) 0.91
Masking of the Fc region in human IgG4 by constrained X-ray scattering modelling: implications for antibody function and therapy. Biochem J (2010) 0.91
Directed evolution of transketolase substrate specificity towards an aliphatic aldehyde. J Biotechnol (2008) 0.90
Non-alpha-hydroxylated aldehydes with evolved transketolase enzymes. Org Biomol Chem (2010) 0.90
Effects of fermentation strategy on the characteristics of plasmid DNA production. Biotechnol Appl Biochem (2003) 0.89
Directed evolution of biocatalytic processes. Biomol Eng (2005) 0.89
Molecular Evolution of Plant AAP and LHT Amino Acid Transporters. Front Plant Sci (2012) 0.88
Characterization of oxygen transfer in miniature and lab-scale bubble column bioreactors and comparison of microbial growth performance based on constant k(L)a. Biotechnol Prog (2005) 0.88
'Dopamine-first' mechanism enables the rational engineering of the norcoclaurine synthase aldehyde activity profile. FEBS J (2015) 0.88
Phosphate mediated biomimetic synthesis of tetrahydroisoquinoline alkaloids. Chem Commun (Camb) (2011) 0.87
Next generation maleimides enable the controlled assembly of antibody-drug conjugates via native disulfide bond bridging. Org Biomol Chem (2014) 0.87
Rapid optimization of protein freeze-drying formulations using ultra scale-down and factorial design of experiment in microplates. Biotechnol Bioeng (2009) 0.87
Stabilized integrin-targeting ternary LPD (lipopolyplex) vectors for gene delivery designed to disassemble within the target cell. Bioconjug Chem (2009) 0.87
Protein denaturation and protein:drugs interactions from intrinsic protein fluorescence measurements at the nanolitre scale. Protein Sci (2010) 0.87
Targeted gene delivery to human airway epithelial cells with synthetic vectors incorporating novel targeting peptides selected by phage display. J Drug Target (2004) 0.87
Pasteurellaceae ComE1 proteins combine the properties of fibronectin adhesins and DNA binding competence proteins. PLoS One (2008) 0.87
AtCCX3 is an Arabidopsis endomembrane H+ -dependent K+ transporter. Plant Physiol (2008) 0.87
A mild synthesis of N-functionalised bromomaleimides, thiomaleimides and bromopyridazinediones. Tetrahedron Lett (2013) 0.87
Impact of intrinsic DNA structure on processing of plasmids for gene therapy and DNA vaccines. J Biotechnol (2004) 0.87
Elemental concentrations in the seed of mutants and natural variants of Arabidopsis thaliana grown under varying soil conditions. PLoS One (2013) 0.87
Acid-cleavable thiomaleamic acid linker for homogeneous antibody-drug conjugation. Chem Commun (Camb) (2013) 0.86
Functional native disulfide bridging enables delivery of a potent, stable and targeted antibody-drug conjugate (ADC). Chem Commun (Camb) (2015) 0.86
Distributions of enzyme residues yielding mutants with improved substrate specificities from two different directed evolution strategies. Protein Eng Des Sel (2009) 0.86
The solution structure of rabbit IgG accounts for its interactions with the Fc receptor and complement C1q and its conformational stability. J Mol Biol (2012) 0.86
Convection-enhanced delivery of neprilysin: a novel amyloid-β-degrading therapeutic strategy. J Alzheimers Dis (2012) 0.86
The mechanism of phloem loading in rice (Oryza sativa). Mol Cells (2012) 0.86
Introns control expression of sucrose transporter LeSUT1 in trichomes, companion cells and in guard cells. Plant Mol Biol (2008) 0.85
Bioconjugation of green fluorescent protein via an unexpectedly stable cyclic sulfonium intermediate. Chembiochem (2012) 0.85
Enhanced heterologous expression of two Streptomyces griseolus cytochrome P450s and Streptomyces coelicolor ferredoxin reductase as potentially efficient hydroxylation catalysts. Appl Environ Microbiol (2003) 0.85
Rational substrate and enzyme engineering of transketolase for aromatics. Org Biomol Chem (2012) 0.85
Directed evolution strategies for improved enzymatic performance. Microb Cell Fact (2005) 0.85
Regioselective and stoichiometrically controlled conjugation of photodynamic sensitizers to a HER2 targeting antibody fragment. Bioconjug Chem (2014) 0.84
TTC-based screening assay for ω-transaminases: a rapid method to detect reduction of 2-hydroxy ketones. J Biotechnol (2012) 0.84
A novel fluorescent assay for sucrose transporters. Plant Methods (2012) 0.84
Bioprocess engineering issues that would be faced in producing a DNA vaccine at up to 100 m3 fermentation scale for an influenza pandemic. Biotechnol Prog (2005) 0.84
Receptor-targeted nanocomplexes optimized for gene transfer to primary vascular cells and explant cultures of rabbit aorta. Mol Ther (2008) 0.84
Comparative functional genomic analysis of Pasteurellaceae adhesins using phage display. Vet Microbiol (2007) 0.84
Fusion to GFP blocks intercellular trafficking of the sucrose transporter SUT1 leading to accumulation in companion cells. BMC Plant Biol (2003) 0.84
A high-throughput fluorescence chemical denaturation assay as a general screen for protein-ligand binding. Anal Biochem (2010) 0.84
Nebulisation of receptor-targeted nanocomplexes for gene delivery to the airway epithelium. PLoS One (2011) 0.84
Fluorescence characterization of clinically-important bacteria. PLoS One (2013) 0.84
α,α'-Dihydroxyketone formation using aromatic and heteroaromatic aldehydes with evolved transketolase enzymes. Chem Commun (Camb) (2010) 0.83
Reversible protein affinity-labelling using bromomaleimide-based reagents. Org Biomol Chem (2013) 0.83
Multifunctional receptor-targeted nanocomplexes for magnetic resonance imaging and transfection of tumours. Biomaterials (2012) 0.83
Novel adhesin from Pasteurella multocida that binds to the integrin-binding fibronectin FnIII9-10 repeats. Infect Immun (2007) 0.83
Insights into the PI3-K-PKB-mTOR signalling pathway from small molecules. J Chem Biol (2008) 0.83