Published in Future Med Chem on May 01, 2011
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An active enzyme constructed from a 9-amino acid alphabet. J Biol Chem (2005) 1.12
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Evolution of highly active enzymes by homology-independent recombination. Proc Natl Acad Sci U S A (2005) 1.08
Modifying the stereochemistry of an enzyme-catalyzed reaction by directed evolution. Proc Natl Acad Sci U S A (2003) 1.07
Directed evolution of a thermostable phosphite dehydrogenase for NAD(P)H regeneration. Appl Environ Microbiol (2005) 1.06
Direct conversion of ethane to ethanol by engineered cytochrome P450 BM3. Chembiochem (2005) 1.05
Highly L and D enantioselective variants of horseradish peroxidase discovered by an ultrahigh-throughput selection method. Proc Natl Acad Sci U S A (2008) 1.04
Directed evolution of endoglucanase III (Cel12A) from Trichoderma reesei. Appl Microbiol Biotechnol (2009) 1.03
Autodisplay: efficient bacterial surface display of recombinant proteins. Appl Microbiol Biotechnol (2005) 1.02
Site-saturation mutagenesis is more efficient than DNA shuffling for the directed evolution of beta-fucosidase from beta-galactosidase. J Mol Biol (2005) 1.02
Directed evolution of D-sialic acid aldolase to L-3-deoxy-manno-2-octulosonic acid (L-KDO) aldolase. Proc Natl Acad Sci U S A (2005) 1.01
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Directed evolution of an orthogonal nucleoside analog kinase via fluorescence-activated cell sorting. Nucleic Acids Res (2009) 0.97
A comparison of directed evolution approaches using the beta-glucuronidase model system. J Mol Biol (2003) 0.97
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Further improvement of phosphite dehydrogenase thermostability by saturation mutagenesis. Biotechnol Bioeng (2008) 0.94
Expanding substrate specificity of GT-B fold glycosyltransferase via domain swapping and high-throughput screening. Biotechnol Bioeng (2009) 0.93
Novel methods for biocatalyst screening. Curr Opin Chem Biol (2001) 0.93
The diversity challenge in directed protein evolution. Comb Chem High Throughput Screen (2006) 0.92
Altering the substrate specificity of organophosphorus hydrolase for enhanced hydrolysis of chlorpyrifos. Appl Environ Microbiol (2004) 0.91
Catalytic turnover-based phage selection for engineering the substrate specificity of Sfp phosphopantetheinyl transferase. J Mol Biol (2009) 0.91
Directed evolution of Pseudomonas aeruginosa lipase for improved amide-hydrolyzing activity. Protein Eng Des Sel (2005) 0.90
Directed evolution of the surface chemistry of the reporter enzyme beta-glucuronidase. Nat Biotechnol (1999) 0.89
Design and directed evolution of a dideoxy purine nucleoside phosphorylase. Protein Eng Des Sel (2010) 0.89
Improved catalytic efficiency of endo-beta-1,4-glucanase from Bacillus subtilis BME-15 by directed evolution. Appl Microbiol Biotechnol (2008) 0.88
Engineering of Pseudomonas aeruginosa lipase by directed evolution for enhanced amidase activity: mechanistic implication for amide hydrolysis by serine hydrolases. Protein Eng Des Sel (2007) 0.88
Creation of a tailored aldolase for the parallel synthesis of sialic acid mimetics. Angew Chem Int Ed Engl (2005) 0.87
Directed evolution of transketolase activity on non-phosphorylated substrates. J Biotechnol (2007) 0.87
Engineering novel carotenoids in microorganisms. Curr Opin Biotechnol (2000) 0.87
Two alternative modes for optimizing nylon-6 byproduct hydrolytic activity from a carboxylesterase with a beta-lactamase fold: X-ray crystallographic analysis of directly evolved 6-aminohexanoate-dimer hydrolase. Protein Sci (2009) 0.87
Distributions of enzyme residues yielding mutants with improved substrate specificities from two different directed evolution strategies. Protein Eng Des Sel (2009) 0.86
The evolution of catalytic efficiency and substrate promiscuity in human theta class 1-1 glutathione transferase. J Mol Biol (2006) 0.86
Engineering monolignol 4-O-methyltransferases to modulate lignin biosynthesis. J Biol Chem (2009) 0.86
Directed evolution of Streptomyces clavuligerus deacetoxycephalosporin C synthase for enhancement of penicillin G expansion. Appl Environ Microbiol (2005) 0.85
Selection strategies for improved biocatalysts. FEBS J (2007) 0.83
Specificity of spontaneous mutations induced in mutA mutator cells. Mutat Res (2004) 0.82
Directed evolution of Bacillus subtilis lipase A by use of enantiomeric phosphonate inhibitors: crystal structures and phage display selection. Chembiochem (2006) 0.82
Identification of amino acid residues involved in 4-chloroindole 3-hydroxylation by cytochrome P450 2A6 using screening of random libraries. J Biotechnol (2008) 0.82
Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase. Protein Eng (2001) 0.81
Changing the substrate specificity of cytochrome c peroxidase using directed evolution. Biochem Biophys Res Commun (2001) 0.81
A new approach to random mutagenesis in vitro. Biotechnol Bioeng (2004) 0.79
Investigation of the mechanism of resistance to third-generation cephalosporins by class C beta-lactamases by using chemical complementation. Chembiochem (2005) 0.77
A genomics-guided approach for discovering and expressing cryptic metabolic pathways. Nat Biotechnol (2003) 2.41
The calicheamicin gene cluster and its iterative type I enediyne PKS. Science (2002) 2.40
Microbial genomics as a guide to drug discovery and structural elucidation: ECO-02301, a novel antifungal agent, as an example. J Nat Prod (2005) 1.88
The IsdG-family of haem oxygenases degrades haem to a novel chromophore. Mol Microbiol (2010) 1.40
Benzodiazepine biosynthesis in Streptomyces refuineus. Chem Biol (2007) 1.21
Antimicrobial drug resistance affects broad changes in metabolomic phenotype in addition to secondary metabolism. Proc Natl Acad Sci U S A (2013) 1.07
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A unifying nitrososynthase involved in nitrosugar biosynthesis. J Am Chem Soc (2008) 0.96
Phenotypic mapping of metabolic profiles using self-organizing maps of high-dimensional mass spectrometry data. Anal Chem (2014) 0.93
Combined chemical and biosynthetic route to access a new apoptolidin congener. Org Lett (2009) 0.91
Design and directed evolution of a dideoxy purine nucleoside phosphorylase. Protein Eng Des Sel (2010) 0.89
Crystal structure of a phosphonotripeptide K-26 in complex with angiotensin converting enzyme homologue (AnCE) from Drosophila melanogaster. Biochem Biophys Res Commun (2010) 0.89
Bioactive oligosaccharide natural products. Nat Prod Rep (2014) 0.89
Biosynthesis of the Apoptolidins in Nocardiopsis sp. FU 40. Tetrahedron (2011) 0.88
Structural mass spectrometry: rapid methods for separation and analysis of peptide natural products. J Nat Prod (2012) 0.88
Structure and mechanism of ORF36, an amino sugar oxidizing enzyme in everninomicin biosynthesis . Biochemistry (2010) 0.87
Phosphonopeptide K-26 biosynthetic intermediates in Astrosporangium hypotensionis. Chem Commun (Camb) (2006) 0.87
Five-component cascade synthesis of nucleotide analogues in an engineered self-immobilized enzyme aggregate. Chembiochem (2010) 0.86
Adenylation enzyme characterization using gamma -(18)O(4)-ATP pyrophosphate exchange. Chem Biol (2009) 0.86
Hops (Humulus lupulus) inhibits oxidative estrogen metabolism and estrogen-induced malignant transformation in human mammary epithelial cells (MCF-10A). Cancer Prev Res (Phila) (2011) 0.85
Identification of ACE pharmacophore in the phosphonopeptide metabolite K-26. Bioorg Med Chem Lett (2007) 0.83
Reassembly of anthramycin biosynthetic gene cluster by using recombinogenic cassettes. Chembiochem (2008) 0.83
Bacillus cereus phosphopentomutase is an alkaline phosphatase family member that exhibits an altered entry point into the catalytic cycle. J Biol Chem (2010) 0.83
Fluorescent probes of the apoptolidins and their utility in cellular localization studies. Angew Chem Int Ed Engl (2014) 0.81
Synthesis of nucleotide analogues by a promiscuous phosphoribosyltransferase. Org Lett (2007) 0.80
Crystallization and preliminary X-ray analysis of a phosphopentomutase from Bacillus cereus. Acta Crystallogr Sect F Struct Biol Cryst Commun (2010) 0.80
Nitrososynthase-triggered oxidative carbon-carbon bond cleavage in baumycin biosynthesis. J Am Chem Soc (2013) 0.79
Biocatalysis in pharmaceutical preparation and alteration. Curr Opin Chem Biol (2006) 0.77
Molecular differences between a mutase and a phosphatase: investigations of the activation step in Bacillus cereus phosphopentomutase. Biochemistry (2012) 0.77
Evolution of the antibiotic resistance protein, FosA, is linked to a catalytically promiscuous progenitor. Biochemistry (2009) 0.77
Decoding chemical structures from genomes. Nat Chem Biol (2005) 0.76
SERMs attenuate estrogen-induced malignant transformation of human mammary epithelial cells by upregulating detoxification of oxidative metabolites. Cancer Prev Res (Phila) (2014) 0.76
Lactococcus lactis fabH, encoding beta-ketoacyl-acyl carrier protein synthase, can be functionally replaced by the Plasmodium falciparum congener. Appl Environ Microbiol (2010) 0.75
Distance geometry protocol to generate conformations of natural products to structurally interpret ion mobility-mass spectrometry collision cross sections. J Phys Chem B (2014) 0.75
Characterisation of a bacterial galactokinase with high activity and broad substrate tolerance for chemoenzymatic synthesis of 6-aminogalactose-1-phosphate and analogues. Chembiochem (2017) 0.75
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