A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes.

PubWeight™: 2.67‹?› | Rank: Top 1%

🔗 View Article (PMID 18601270)

Published in Angew Chem Int Ed Engl on January 01, 2008

Authors

Jan Vogelsang1, Robert Kasper, Christian Steinhauer, Britta Person, Mike Heilemann, Markus Sauer, Philip Tinnefeld

Author Affiliations

1: Angewandte Physik-Biophysik, and Center for NanoScience, Ludwig-Maximilians-Universität, Amalienstrasse 54, 80799 München, Germany.

Articles citing this

A guide to super-resolution fluorescence microscopy. J Cell Biol (2010) 4.71

Cyanine fluorophore derivatives with enhanced photostability. Nat Methods (2011) 2.85

Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging. Annu Rev Phys Chem (2012) 2.19

Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy. Proc Natl Acad Sci U S A (2009) 2.12

Mitigating unwanted photophysical processes for improved single-molecule fluorescence imaging. Biophys J (2009) 2.05

The large conformational changes of Hsp90 are only weakly coupled to ATP hydrolysis. Nat Struct Mol Biol (2009) 2.03

Subnanometre single-molecule localization, registration and distance measurements. Nature (2010) 1.98

Multicolor fluorescence nanoscopy in fixed and living cells by exciting conventional fluorophores with a single wavelength. Biophys J (2010) 1.74

Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT. Nat Methods (2014) 1.70

Ultrabright photoactivatable fluorophores created by reductive caging. Nat Methods (2012) 1.57

Quantitative localization microscopy: effects of photophysics and labeling stoichiometry. PLoS One (2015) 1.47

Advances in fluorescence labeling strategies for dynamic cellular imaging. Nat Chem Biol (2014) 1.47

Ultra-stable organic fluorophores for single-molecule research. Chem Soc Rev (2014) 1.31

Acidification of the oxygen scavenging system in single-molecule fluorescence studies: in situ sensing with a ratiometric dual-emission probe. Anal Chem (2010) 1.29

Superresolution optical fluctuation imaging with organic dyes. Angew Chem Int Ed Engl (2010) 1.24

Eight years of single-molecule localization microscopy. Histochem Cell Biol (2014) 1.24

Microfluidic device for single-molecule experiments with enhanced photostability. J Am Chem Soc (2009) 1.20

Single-molecule fluorescence probes dynamics of barrier crossing. Nature (2013) 1.15

Single-molecule FRET of protein-nucleic acid and protein-protein complexes: surface passivation and immobilization. Methods (2010) 1.11

Supramolecular assemblies underpin turnover of outer membrane proteins in bacteria. Nature (2015) 1.04

The bright future of single-molecule fluorescence imaging. Curr Opin Chem Biol (2014) 1.03

Dendrimer probes for enhanced photostability and localization in fluorescence imaging. Biophys J (2013) 0.99

Quantifying heterogeneity and conformational dynamics from single molecule FRET of diffusing molecules: recurrence analysis of single particles (RASP). Phys Chem Chem Phys (2011) 0.98

The changing point-spread function: single-molecule-based super-resolution imaging. Histochem Cell Biol (2014) 0.96

Accelerated single photon emission from dye molecule-driven nanoantennas assembled on DNA. Nat Commun (2012) 0.95

Phosphine quenching of cyanine dyes as a versatile tool for fluorescence microscopy. J Am Chem Soc (2013) 0.94

The contribution of reactive oxygen species to the photobleaching of organic fluorophores. Photochem Photobiol (2013) 0.94

Ultrafast, temporally stochastic STED nanoscopy of millisecond dynamics. Nat Methods (2015) 0.94

Molecular architecture of native fibronectin fibrils. Nat Commun (2015) 0.94

Visualizing helicases unwinding DNA at the single molecule level. Nucleic Acids Res (2010) 0.93

Opportunities and challenges in single-molecule and single-particle fluorescence microscopy for mechanistic studies of chemical reactions. Nat Chem (2013) 0.92

Identifying mechanisms of interfacial dynamics using single-molecule tracking. Langmuir (2012) 0.90

FRET-enabled optical modulation for high sensitivity fluorescence imaging. J Am Chem Soc (2010) 0.90

Multicolor single-molecule FRET to explore protein folding and binding. Mol Biosyst (2010) 0.90

Sequential superresolution imaging of multiple targets using a single fluorophore. PLoS One (2015) 0.89

Receptor dimerization dynamics as a regulatory valve for plasticity of type I interferon signaling. J Cell Biol (2015) 0.88

Triplet imaging of oxygen consumption during the contraction of a single smooth muscle cell (A7r5). Biophys J (2010) 0.87

Achieving λ/10 resolution CW STED nanoscopy with a Ti:Sapphire oscillator. PLoS One (2012) 0.86

Measuring ultrafast protein folding rates from photon-by-photon analysis of single molecule fluorescence trajectories. Chem Phys (2013) 0.86

Optical saturation as a versatile tool to enhance resolution in confocal microscopy. Biophys J (2009) 0.85

Fluorescent labeling and modification of proteins. J Chem Biol (2013) 0.85

DNA hairpin stabilization on a hydrophobic surface. Small (2012) 0.85

Thioamide quenching of fluorescent probes through photoinduced electron transfer: mechanistic studies and applications. J Am Chem Soc (2013) 0.85

Quantum yield and excitation rate of single molecules close to metallic nanostructures. Nat Commun (2014) 0.85

Live cell micropatterning reveals the dynamics of signaling complexes at the plasma membrane. J Cell Biol (2014) 0.84

Does super-resolution fluorescence microscopy obsolete previous microscopic approaches to protein co-localization? Methods Mol Biol (2015) 0.84

Chromophore photoreduction in red fluorescent proteins is responsible for bleaching and phototoxicity. J Phys Chem B (2014) 0.84

Mapping molecules in scanning far-field fluorescence nanoscopy. Nat Commun (2015) 0.83

Fluorescent Polymer Nanoparticles Based on Dyes: Seeking Brighter Tools for Bioimaging. Small (2016) 0.82

Fast single-molecule FRET spectroscopy: theory and experiment. Phys Chem Chem Phys (2014) 0.82

A dendritic single-molecule fluorescent probe that is monovalent, photostable and minimally blinking. Nat Chem (2013) 0.82

Photobleaching lifetimes of cyanine fluorophores used for single-molecule Förster resonance energy transfer in the presence of various photoprotection systems. Chembiochem (2013) 0.82

Helicase-mediated changes in RNA structure at the single-molecule level. RNA Biol (2013) 0.82

Single molecule fluorescence detection and tracking in mammalian cells: the state-of-the-art and future perspectives. Int J Mol Sci (2012) 0.82

A new method for inferring hidden markov models from noisy time sequences. PLoS One (2012) 0.82

Resonant Scanning with Large Field of View Reduces Photobleaching and Enhances Fluorescence Yield in STED Microscopy. Sci Rep (2015) 0.81

Instructive roles for cytokine-receptor binding parameters in determining signaling and functional potency. Sci Signal (2015) 0.80

Conformational transitions in the glycine-bound GluN1 NMDA receptor LBD via single-molecule FRET. Biophys J (2015) 0.80

Model-independent counting of molecules in single-molecule localization microscopy. Mol Biol Cell (2016) 0.80

Optimizing Imaging Conditions for Demanding Multi-Color Super Resolution Localization Microscopy. PLoS One (2016) 0.79

A novel tri-enzyme system in combination with laser-driven NMR enables efficient nuclear polarization of biomolecules in solution. J Phys Chem B (2013) 0.79

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation. Cell (2017) 0.78

Strong signal increase in STED fluorescence microscopy by imaging regions of subdiffraction extent. Proc Natl Acad Sci U S A (2017) 0.78

Automated Analysis of Single-Molecule Photobleaching Data by Statistical Modeling of Spot Populations. Biophys J (2015) 0.78

A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization. Nat Commun (2016) 0.78

Studying DNA-protein interactions with single-molecule Förster resonance energy transfer. Protoplasma (2013) 0.78

In cellulo evaluation of phototransformation quantum yields in fluorescent proteins used as markers for single-molecule localization microscopy. PLoS One (2014) 0.78

Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling. Nature (2017) 0.77

Microfluidics-based selection of red-fluorescent proteins with decreased rates of photobleaching. Integr Biol (Camb) (2015) 0.77

Direct quantification of microRNA at low picomolar level in sera of glioma patients using a competitive hybridization followed by amplified voltammetric detection. Anal Chem (2012) 0.77

Localization-based super-resolution imaging of cellular structures. Methods Mol Biol (2013) 0.77

Coclustering of ErbB1 and ErbB2 revealed by FRET-sensitized acceptor bleaching. Biophys J (2010) 0.77

Biocompatible fluorescent silicon nanocrystals for single-molecule tracking and fluorescence imaging. J Cell Biol (2013) 0.77

Electronic tuning of self-healing fluorophores for live-cell and single-molecule imaging. Chem Sci (2016) 0.77

Characterization of dark quencher chromophores as nonfluorescent acceptors for single-molecule FRET. Biophys J (2012) 0.77

In vitro RNA release from a human rhinovirus monitored by means of a molecular beacon and chip electrophoresis. Anal Bioanal Chem (2016) 0.77

From single molecules to life: microscopy at the nanoscale. Anal Bioanal Chem (2016) 0.76

Intra-molecular triplet energy transfer is a general approach to improve organic fluorophore photostability. Photochem Photobiol Sci (2015) 0.76

STAT2 is an essential adaptor in USP18-mediated suppression of type I interferon signaling. Nat Struct Mol Biol (2017) 0.76

Molecular approaches to chromatography using single molecule spectroscopy. Anal Chem (2014) 0.75

Spectral properties of single gold nanoparticles in close proximity to biological fluorophores excited by 2-photon excitation. PLoS One (2015) 0.75

Simple method to enhance the photostability of the fluorescence reporter R6G for prolonged single-molecule studies. J Phys Chem A (2013) 0.75

Optimization of Cyanine Dye Stability and Analysis of FRET Interaction on DNA Microarrays. Biology (Basel) (2016) 0.75

An in-vitro tag-and-modify protein sample generation method for singlemolecule FRET. J Biol Chem (2017) 0.75

Single-molecule Förster resonance energy transfer studies of RNA structure, dynamics and function. Biophys Rev (2009) 0.75

Photostable and photoswitching fluorescent dyes for super-resolution imaging. J Biol Inorg Chem (2017) 0.75

Chromophore Renewal and Fluorogen-Binding Tags: A Match Made to Last. Sci Rep (2017) 0.75

Articles by these authors

Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angew Chem Int Ed Engl (2008) 7.15

Carbocyanine dyes as efficient reversible single-molecule optical switch. J Am Chem Soc (2005) 5.56

Direct stochastic optical reconstruction microscopy with standard fluorescent probes. Nat Protoc (2011) 3.07

Live-cell super-resolution imaging with trimethoprim conjugates. Nat Methods (2010) 2.94

Super-resolution imaging with small organic fluorophores. Angew Chem Int Ed Engl (2009) 2.80

Reconfigurable, braced, three-dimensional DNA nanostructures. Nat Nanotechnol (2008) 2.49

rapidSTORM: accurate, fast open-source software for localization microscopy. Nat Methods (2012) 2.22

Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging. Annu Rev Phys Chem (2012) 2.19

Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy. Proc Natl Acad Sci U S A (2009) 2.12

Super-resolution imaging visualizes the eightfold symmetry of gp210 proteins around the nuclear pore complex and resolves the central channel with nanometer resolution. J Cell Sci (2012) 1.94

Fluorescence quenching by photoinduced electron transfer: a reporter for conformational dynamics of macromolecules. Chemphyschem (2009) 1.90

Direct observation of abortive initiation and promoter escape within single immobilized transcription complexes. Biophys J (2005) 1.90

Branching out of single-molecule fluorescence spectroscopy: challenges for chemistry and influence on biology. Angew Chem Int Ed Engl (2005) 1.86

On the mechanism of Trolox as antiblinking and antibleaching reagent. J Am Chem Soc (2009) 1.86

Inter- and intramolecular fluorescence quenching of organic dyes by tryptophan. Bioconjug Chem (2003) 1.83

A microscopic view of miniprotein folding: enhanced folding efficiency through formation of an intermediate. Proc Natl Acad Sci U S A (2005) 1.82

Superresolution microscopy on the basis of engineered dark states. J Am Chem Soc (2008) 1.81

Single-molecule kinetics and super-resolution microscopy by fluorescence imaging of transient binding on DNA origami. Nano Lett (2010) 1.79

Live-cell dSTORM with SNAP-tag fusion proteins. Nat Methods (2011) 1.75

A close look at fluorescence quenching of organic dyes by tryptophan. Chemphyschem (2005) 1.52

Click chemistry for the conservation of cellular structures and fluorescent proteins: ClickOx. Biotechnol J (2014) 1.52

DNA origami as a nanoscopic ruler for super-resolution microscopy. Angew Chem Int Ed Engl (2009) 1.49

Spectroscopic study and evaluation of red-absorbing fluorescent dyes. Bioconjug Chem (2003) 1.48

p53 family members in myogenic differentiation and rhabdomyosarcoma development. Cancer Cell (2006) 1.47

Live-cell super-resolution imaging with synthetic fluorophores. Annu Rev Phys Chem (2012) 1.47

DNA binding cooperativity of p53 modulates the decision between cell-cycle arrest and apoptosis. Mol Cell (2010) 1.47

Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging. Photochem Photobiol Sci (2010) 1.46

Quantitative single-molecule microscopy reveals that CENP-A(Cnp1) deposition occurs during G2 in fission yeast. Open Biol (2012) 1.42

Fluorescence quenching of dyes by tryptophan: interactions at atomic detail from combination of experiment and computer simulation. J Am Chem Soc (2003) 1.42

The initial step of DNA hairpin folding: a kinetic analysis using fluorescence correlation spectroscopy. Nucleic Acids Res (2006) 1.38

In situ measurements of the formation and morphology of intracellular β-amyloid fibrils by super-resolution fluorescence imaging. J Am Chem Soc (2011) 1.37

Probing polyproline structure and dynamics by photoinduced electron transfer provides evidence for deviations from a regular polyproline type II helix. Proc Natl Acad Sci U S A (2007) 1.37

Make them blink: probes for super-resolution microscopy. Chemphyschem (2010) 1.36

Measurement of submicrosecond intramolecular contact formation in peptides at the single-molecule level. J Am Chem Soc (2003) 1.35

Multistep energy transfer in single molecular photonic wires. J Am Chem Soc (2004) 1.35

Multicolor photoswitching microscopy for subdiffraction-resolution fluorescence imaging. Photochem Photobiol Sci (2009) 1.26

Counting molecules in single organelles with superresolution microscopy allows tracking of the endosome maturation trajectory. Proc Natl Acad Sci U S A (2013) 1.26

Super-resolution microscopy reveals specific recruitment of HIV-1 envelope proteins to viral assembly sites dependent on the envelope C-terminal tail. PLoS Pathog (2013) 1.25

'Self-healing' dyes: intramolecular stabilization of organic fluorophores. Nat Methods (2012) 1.25

Superresolution optical fluctuation imaging with organic dyes. Angew Chem Int Ed Engl (2010) 1.24

The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. J Biotechnol (2010) 1.24

Eight years of single-molecule localization microscopy. Histochem Cell Biol (2014) 1.24

Dynamics of unfolded polypeptide chains in crowded environment studied by fluorescence correlation spectroscopy. J Mol Biol (2006) 1.23

Application of multiline two-photon microscopy to functional in vivo imaging. J Neurosci Methods (2006) 1.23

Coordinate-based colocalization analysis of single-molecule localization microscopy data. Histochem Cell Biol (2011) 1.23

Chemically induced photoswitching of fluorescent probes--a general concept for super-resolution microscopy. Molecules (2011) 1.22

Multiscale spatial organization of RNA polymerase in Escherichia coli. Biophys J (2013) 1.20

Fluorescence resonance energy transfer (FRET) and competing processes in donor-acceptor substituted DNA strands: a comparative study of ensemble and single-molecule data. J Biotechnol (2002) 1.20

Three-dimensional, tomographic super-resolution fluorescence imaging of serially sectioned thick samples. PLoS One (2012) 1.18

Distance dependence of single-fluorophore quenching by gold nanoparticles studied on DNA origami. ACS Nano (2012) 1.17

Single-molecule FRET ruler based on rigid DNA origami blocks. Chemphyschem (2011) 1.17

Super-resolution imaging reveals the internal architecture of nano-sized syntaxin clusters. J Biol Chem (2012) 1.15

High-resolution colocalization of single dye molecules by fluorescence lifetime imaging microscopy. Anal Chem (2002) 1.13

Measuring localization performance of super-resolution algorithms on very active samples. Opt Express (2011) 1.12

Resolving single-molecule assembled patterns with superresolution blink-microscopy. Nano Lett (2010) 1.11

Polymer properties of polythymine as revealed by translational diffusion. Biophys J (2007) 1.11

A simple method to estimate the average localization precision of a single-molecule localization microscopy experiment. Histochem Cell Biol (2014) 1.10

Single-molecule STED microscopy with photostable organic fluorophores. Small (2010) 1.10

Defective proteolytic systems in Mybpc3-targeted mice with cardiac hypertrophy. Basic Res Cardiol (2011) 1.10

Monitoring multiple distances within a single molecule using switchable FRET. Nat Methods (2010) 1.09

Single-molecule four-color FRET visualizes energy-transfer paths on DNA origami. J Am Chem Soc (2011) 1.07

Colocalization and FRET-analysis of subunits c and a of the vacuolar H+-ATPase in living plant cells. J Biotechnol (2004) 1.07

Radiative and nonradiative rate fluctuations of single colloidal semiconductor nanocrystals. J Phys Chem B (2006) 1.04

Super-resolution fluorescence imaging of chromosomal DNA. J Struct Biol (2011) 1.04

Fluorescent proteins for single-molecule fluorescence applications. J Biophotonics (2008) 1.03

Fluorescence of single molecules in polymer films: sensitivity of blinking to local environment. J Phys Chem B (2007) 1.03

Fluorescence and super-resolution standards based on DNA origami. Nat Methods (2012) 1.02

Cellular uptake studies with beta-peptides. Chembiochem (2002) 1.02