Published in Protoplasma on December 28, 2013
Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature (1953) 47.27
Surfing the p53 network. Nature (2000) 35.36
Atomic force microscope. Phys Rev Lett (1986) 32.69
Energy transfer: a spectroscopic ruler. Proc Natl Acad Sci U S A (1967) 11.78
Fluorescence spectroscopy of single biomolecules. Science (1999) 10.67
Structural basis of transcription: RNA polymerase II at 2.8 angstrom resolution. Science (2001) 8.92
A practical guide to single-molecule FRET. Nat Methods (2008) 8.62
Structural basis of transcription: an RNA polymerase II elongation complex at 3.3 A resolution. Science (2001) 8.24
Fluorescence resonance energy transfer and nucleic acids. Methods Enzymol (1992) 8.22
Enzymatic synthesis of deoxyribonucleic acid. I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. J Biol Chem (1958) 7.87
Zero-mode waveguides for single-molecule analysis at high concentrations. Science (2003) 7.69
Probing the interaction between two single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor. Proc Natl Acad Sci U S A (1996) 7.65
FRET imaging. Nat Biotechnol (2003) 6.57
Nonblinking and long-lasting single-molecule fluorescence imaging. Nat Methods (2006) 6.25
Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 A resolution. Cell (1999) 5.88
Analysis of single-molecule FRET trajectories using hidden Markov modeling. Biophys J (2006) 5.52
The orientational freedom of molecular probes. The orientation factor in intramolecular energy transfer. Biophys J (1979) 5.24
Structural basis of transcription initiation: RNA polymerase holoenzyme at 4 A resolution. Science (2002) 5.18
Initiation and re-initiation of DNA unwinding by the Escherichia coli Rep helicase. Nature (2002) 5.13
Initial transcription by RNA polymerase proceeds through a DNA-scrunching mechanism. Science (2006) 4.36
Selective elimination of the exonuclease activity of the deoxyribonucleic acid polymerase from Escherichia coli B by limited proteolysis. Proc Natl Acad Sci U S A (1970) 4.25
Fluorescence-aided molecule sorting: analysis of structure and interactions by alternating-laser excitation of single molecules. Proc Natl Acad Sci U S A (2004) 4.06
Structure and mechanism of the RNA polymerase II transcription machinery. Nat Struct Mol Biol (2004) 4.03
New directions in single-molecule imaging and analysis. Proc Natl Acad Sci U S A (2007) 3.83
Repetitive shuttling of a motor protein on DNA. Nature (2005) 3.82
Spring-loaded mechanism of DNA unwinding by hepatitis C virus NS3 helicase. Science (2007) 3.70
Processive DNA synthesis observed in a polymerase crystal suggests a mechanism for the prevention of frameshift mutations. Proc Natl Acad Sci U S A (2003) 3.67
Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Förster distance dependence and subpopulations. Proc Natl Acad Sci U S A (1999) 3.61
Accurate FRET measurements within single diffusing biomolecules using alternating-laser excitation. Biophys J (2005) 3.22
A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes. Angew Chem Int Ed Engl (2008) 2.67
RNA chain initiation by Escherichia coli RNA polymerase. Structural transitions of the enzyme in early ternary complexes. Biochemistry (1989) 2.63
Interaction of RNA polymerase with lacUV5 promoter DNA during mRNA initiation and elongation. Footprinting, methylation, and rifampicin-sensitivity changes accompanying transcription initiation. J Mol Biol (1985) 2.59
Shot-noise limited single-molecule FRET histograms: comparison between theory and experiments. J Phys Chem B (2006) 2.58
Do-it-yourself guide: how to use the modern single-molecule toolkit. Nat Methods (2008) 2.52
Distribution of end-to-end distances of oligopeptides in solution as estimated by energy transfer. Proc Natl Acad Sci U S A (1975) 2.50
Mechanism of bacterial transcription initiation: RNA polymerase - promoter binding, isomerization to initiation-competent open complexes, and initiation of RNA synthesis. J Mol Biol (2011) 2.50
Fluorescence-force spectroscopy maps two-dimensional reaction landscape of the holliday junction. Science (2007) 2.49
Multiparameter single-molecule fluorescence spectroscopy reveals heterogeneity of HIV-1 reverse transcriptase:primer/template complexes. Proc Natl Acad Sci U S A (2003) 2.48
DNA-binding orientation and domain conformation of the E. coli rep helicase monomer bound to a partial duplex junction: single-molecule studies of fluorescently labeled enzymes. J Mol Biol (2004) 2.40
The role of the transcription bubble and TFIIB in promoter clearance by RNA polymerase II. Mol Cell (2005) 2.31
Structural basis of transcription initiation. Science (2012) 2.23
Promoter clearance and escape in prokaryotes. Biochim Biophys Acta (2002) 2.20
Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging. Annu Rev Phys Chem (2012) 2.19
Fingers-closing and other rapid conformational changes in DNA polymerase I (Klenow fragment) and their role in nucleotide selectivity. Biochemistry (2008) 2.14
Conformational transitions in DNA polymerase I revealed by single-molecule FRET. Proc Natl Acad Sci U S A (2009) 2.10
Single-molecule biophysics: at the interface of biology, physics and chemistry. J R Soc Interface (2008) 2.06
Escherichia coli DNA helicases: mechanisms of DNA unwinding. Mol Microbiol (1992) 2.03
A stressed intermediate in the formation of stably initiated RNA chains at the Escherichia coli lac UV5 promoter. J Mol Biol (1987) 2.00
Separating structural heterogeneities from stochastic variations in fluorescence resonance energy transfer distributions via photon distribution analysis. J Phys Chem B (2006) 1.94
On the mechanism of Trolox as antiblinking and antibleaching reagent. J Am Chem Soc (2009) 1.86
A nano-positioning system for macromolecular structural analysis. Nat Methods (2008) 1.84
Single-molecule optomechanical cycle. Science (2002) 1.83
Biocompatible force sensor with optical readout and dimensions of 6 nm3. Nano Lett (2005) 1.82
Protein induced fluorescence enhancement as a single molecule assay with short distance sensitivity. Proc Natl Acad Sci U S A (2011) 1.79
Interlaced optical force-fluorescence measurements for single molecule biophysics. Biophys J (2006) 1.75
Opening and closing of the bacterial RNA polymerase clamp. Science (2012) 1.73
Single-molecule tracking of mRNA exiting from RNA polymerase II. Proc Natl Acad Sci U S A (2007) 1.59
A toolkit and benchmark study for FRET-restrained high-precision structural modeling. Nat Methods (2012) 1.56
SSB functions as a sliding platform that migrates on DNA via reptation. Cell (2011) 1.53
Combined optical trapping and single-molecule fluorescence. J Biol (2003) 1.52
Biology, one molecule at a time. Trends Biochem Sci (2009) 1.43
The structure of a high fidelity DNA polymerase bound to a mismatched nucleotide reveals an "ajar" intermediate conformation in the nucleotide selection mechanism. J Biol Chem (2011) 1.43
Defining the limits of single-molecule FRET resolution in TIRF microscopy. Biophys J (2010) 1.43
Detecting force-induced molecular transitions with fluorescence resonant energy transfer. Angew Chem Int Ed Engl (2007) 1.39
Single-molecule detection and identification of multiple species by multiparameter fluorescence detection. Anal Chem (2006) 1.38
Ultra-stable organic fluorophores for single-molecule research. Chem Soc Rev (2014) 1.31
Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave. Proc Natl Acad Sci U S A (2004) 1.30
The Rpb4 subunit of RNA polymerase II contributes to cotranscriptional recruitment of 3' processing factors. Mol Cell Biol (2008) 1.29
Single-molecule measurements of synthesis by DNA polymerase with base-pair resolution. Proc Natl Acad Sci U S A (2009) 1.27
The way to NMR structures of proteins. Nat Struct Biol (2001) 1.17
Solution NMR of large molecules and assemblies. Biochemistry (2007) 1.17
Single-molecule Förster resonance energy transfer reveals an innate fidelity checkpoint in DNA polymerase I. J Am Chem Soc (2012) 1.16
Characterizing single-molecule FRET dynamics with probability distribution analysis. Chemphyschem (2010) 1.14
Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis. Biophys J (2011) 1.08
Protein induced fluorescence enhancement (PIFE) for probing protein-nucleic acid interactions. Chem Soc Rev (2014) 1.07
Competition of Escherichia coli DNA polymerases I, II and III with DNA Pol IV in stressed cells. PLoS One (2010) 1.07
Probability distribution analysis of single-molecule fluorescence anisotropy and resonance energy transfer. J Phys Chem B (2007) 1.07
Conformational landscapes of DNA polymerase I and mutator derivatives establish fidelity checkpoints for nucleotide insertion. Nat Commun (2013) 1.06
Minute negative superhelicity is sufficient to induce the B-Z transition in the presence of low tension. Proc Natl Acad Sci U S A (2010) 1.06
Insight into helicase mechanism and function revealed through single-molecule approaches. Q Rev Biophys (2010) 1.03
Characterizing multiple molecular States in single-molecule multiparameter fluorescence detection by probability distribution analysis. J Phys Chem B (2008) 1.02
The DNA-gate of Bacillus subtilis gyrase is predominantly in the closed conformation during the DNA supercoiling reaction. Proc Natl Acad Sci U S A (2009) 1.02
Mechanical unfolding of human telomere G-quadruplex DNA probed by integrated fluorescence and magnetic tweezers spectroscopy. Nucleic Acids Res (2013) 1.01
Single-molecule measurements of the opening and closing of the DNA gate by eukaryotic topoisomerase II. Proc Natl Acad Sci U S A (2007) 1.01
Alternating-laser excitation: single-molecule FRET and beyond. Chem Soc Rev (2014) 1.01
Single-molecule microscopy reveals new insights into nucleotide selection by DNA polymerase I. Nucleic Acids Res (2012) 1.01
Dynamic architecture of a minimal RNA polymerase II open promoter complex. Mol Cell (2012) 1.01
Capturing reaction paths and intermediates in Cre-loxP recombination using single-molecule fluorescence. Proc Natl Acad Sci U S A (2012) 1.00
DNA cleavage and opening reactions of human topoisomerase IIα are regulated via Mg2+-mediated dynamic bending of gate-DNA. Proc Natl Acad Sci U S A (2012) 0.99
Application of the nano-positioning system to the analysis of fluorescence resonance energy transfer networks. J Phys Chem B (2011) 0.98
Surfing on a new wave of single-molecule fluorescence methods. Phys Biol (2010) 0.95
Single-molecule nanometry for biological physics. Rep Prog Phys (2012) 0.93
Nonnucleoside inhibitor binding affects the interactions of the fingers subdomain of human immunodeficiency virus type 1 reverse transcriptase with DNA. J Virol (2004) 0.92
Breaking the concentration limit of optical single-molecule detection. Chem Soc Rev (2013) 0.91
Sensing DNA opening in transcription using quenchable Förster resonance energy transfer. Biochemistry (2010) 0.91
Dynamics of site switching in DNA polymerase. J Am Chem Soc (2013) 0.90
Guiding strand passage: DNA-induced movement of the gyrase C-terminal domains defines an early step in the supercoiling cycle. Nucleic Acids Res (2011) 0.89
Single-molecule DNA biosensors for protein and ligand detection. Angew Chem Int Ed Engl (2010) 0.89
Sizing up single-molecule enzymatic conformational dynamics. Chem Soc Rev (2014) 0.89
Conformational transitions during FtsK translocase activation of individual XerCD-dif recombination complexes. Proc Natl Acad Sci U S A (2013) 0.88
Conformational transitions in DNA polymerase I revealed by single-molecule FRET. Proc Natl Acad Sci U S A (2009) 2.10
Defining the limits of single-molecule FRET resolution in TIRF microscopy. Biophys J (2010) 1.43
Identifying molecular dynamics in single-molecule FRET experiments with burst variance analysis. Biophys J (2011) 1.08
Recurrence and photon statistics in fluorescence fluctuation spectroscopy. Phys Rev Lett (2004) 0.80
Characterization of dark quencher chromophores as nonfluorescent acceptors for single-molecule FRET. Biophys J (2012) 0.77
Camera-based single-molecule FRET detection with improved time resolution. Phys Chem Chem Phys (2015) 0.76