Published in Chembiochem on September 22, 2008
In-cell fluorescence activation and labeling of proteins mediated by FRET-quenched split inteins. J Am Chem Soc (2012) 0.95
Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. J Biol Chem (2012) 0.94
Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human cells using compact expression cassettes. Sci Rep (2015) 0.90
Photomodulation of protein trans-splicing through backbone photocaging of the DnaE split intein. Chembiochem (2010) 0.89
Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein. PLoS One (2009) 0.84
SPLICEFINDER - a fast and easy screening method for active protein trans-splicing positions. PLoS One (2013) 0.75
Protein Chemical Modification Inside Living Cells Using Split Inteins. Methods Mol Biol (2017) 0.75
An Unprecedented Combination of Serine and Cysteine Nucleophiles in a Split Intein with an Atypical Split Site. J Biol Chem (2015) 0.75
Regeneration of misprimed nonribosomal peptide synthetases by type II thioesterases. Proc Natl Acad Sci U S A (2002) 1.64
The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction. FEBS Lett (2009) 1.63
Chemically induced and light-independent cryptochrome photoreceptor activation. Mol Plant (2007) 1.09
Recent progress in intein research: from mechanism to directed evolution and applications. Cell Mol Life Sci (2012) 1.03
Highly efficient and more general cis- and trans-splicing inteins through sequential directed evolution. J Biol Chem (2011) 1.01
Ligation of a synthetic peptide to the N terminus of a recombinant protein using semisynthetic protein trans-splicing. Angew Chem Int Ed Engl (2006) 1.01
Interaction studies and alanine scanning analysis of a semi-synthetic split intein reveal thiazoline ring formation from an intermediate of the protein splicing reaction. J Biol Chem (2008) 0.99
Engineering artificially split inteins for applications in protein chemistry: biochemical characterization of the split Ssp DnaB intein and comparison to the split Sce VMA intein. Biochemistry (2006) 0.98
In vivo production of artificial nonribosomal peptide products in the heterologous host Escherichia coli. Appl Environ Microbiol (2004) 0.97
Unprecedented rates and efficiencies revealed for new natural split inteins from metagenomic sources. J Biol Chem (2012) 0.94
Generation of site-specific and enzymatically stable conjugates of recombinant proteins with ubiquitin-like modifiers by the Cu(I)-catalyzed azide-alkyne cycloaddition. Chembiochem (2010) 0.94
Click synthesis of ubiquitin dimer analogs to interrogate linkage-specific UBA domain binding. Chem Commun (Camb) (2011) 0.94
Photocontrol of protein activity mediated by the cleavage reaction of a split intein. Angew Chem Int Ed Engl (2011) 0.90
Expanded click conjugation of recombinant proteins with ubiquitin-like modifiers reveals altered substrate preference of SUMO2-modified Ubc9. Angew Chem Int Ed Engl (2011) 0.90
Modification of transmembrane and GPI-anchored proteins on living cells by efficient protein trans-splicing using the Npu DnaE intein. Chem Commun (Camb) (2011) 0.88
Systematic and quantitative analysis of protein-protein recognition between nonribosomal peptide synthetases investigated in the tyrocidine biosynthetic template. Biochemistry (2003) 0.88
Regioselective cysteine bioconjugation by appending a labeled cystein tag to a protein by using protein splicing in trans. Angew Chem Int Ed Engl (2007) 0.87
Bioorthogonal ligation in the spotlight. Angew Chem Int Ed Engl (2009) 0.85
Covalent inhibition of SUMO and ubiquitin-specific cysteine proteases by an in situ thiol-alkyne addition. Bioorg Med Chem (2013) 0.84
An intein-cassette integration approach used for the generation of a split TEV protease activated by conditional protein splicing. Mol Biosyst (2011) 0.84
Generation of a genetically encoded, photoactivatable intein for the controlled production of cyclic peptides. Angew Chem Int Ed Engl (2014) 0.83
Biochemical evidence for conformational changes in the cross-talk between adenylation and peptidyl-carrier protein domains of nonribosomal peptide synthetases. FEBS J (2010) 0.81
Expanding the scope of protein trans-splicing to fragment ligation of an integral membrane protein: towards modulation of porin-based ion channels by chemical modification. Chembiochem (2009) 0.79
Design of an intein that can be inhibited with a small molecule ligand. J Am Chem Soc (2005) 0.78
Structure-activity studies on the upstream splice junction of a semisynthetic intein. Bioorg Med Chem (2013) 0.78
An atypical naturally split intein engineered for highly efficient protein labeling. Angew Chem Int Ed Engl (2014) 0.78
Semisynthesis of proteins using split inteins. Methods Enzymol (2009) 0.77
Numerical construction of estimators for single-molecule fluorescence measurements. J Phys Chem B (2013) 0.77
Chemical-tag labeling of proteins using fully recombinant split inteins. Methods Mol Biol (2015) 0.77
Chemical bypass of intein-catalyzed N-S acyl shift in protein splicing. Angew Chem Int Ed Engl (2013) 0.77
Probing intein-catalyzed thioester formation by unnatural amino acid substitutions in the active site. Biochemistry (2011) 0.77
Protein trans-splicing on an M13 bacteriophage: towards directed evolution of a semisynthetic split intein by phage display. J Pept Sci (2010) 0.77
Kinetics profiling of gramicidin S synthetase A, a member of nonribosomal peptide synthetases. Biochemistry (2014) 0.76
SPLICEFINDER - a fast and easy screening method for active protein trans-splicing positions. PLoS One (2013) 0.75
Extending the scope of site-specific cysteine bioconjugation by appending a prelabeled cysteine tag to proteins using protein trans-splicing. Methods Mol Biol (2011) 0.75
Ligation of synthetic peptides to proteins using semisynthetic protein trans-splicing. Methods Mol Biol (2015) 0.75
Probing the molecular basis of protein function through chemistry. Chembiochem (2006) 0.75
Click-tag and amine-tag: chemical tag approaches for efficient protein labeling in vitro and on live cells using the naturally split Npu DnaE intein. Angew Chem Int Ed Engl (2014) 0.75
Development of a screening system for inteins active in protein splicing based on intein insertion into the LacZα-peptide. Biol Chem (2017) 0.75