A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75.

PubWeight™: 1.89‹?› | Rank: Top 3%

🔗 View Article (PMC 2606027)

Published in PLoS Pathog on January 09, 2009

Authors

Stephen Hare1, Ming-Chieh Shun, Saumya Shree Gupta, Eugene Valkov, Alan Engelman, Peter Cherepanov

Author Affiliations

1: Division of Medicine, Imperial College London, St Mary's Campus, London, United Kingdom.

Articles citing this

Integrase-mediated spacer acquisition during CRISPR-Cas adaptive immunity. Nature (2015) 1.89

HIV DNA integration. Cold Spring Harb Perspect Med (2012) 1.73

LEDGF hybrids efficiently retarget lentiviral integration into heterochromatin. Mol Ther (2010) 1.72

Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration. Proc Natl Acad Sci U S A (2010) 1.68

Multimode, cooperative mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem (2012) 1.55

Structural basis for functional tetramerization of lentiviral integrase. PLoS Pathog (2009) 1.53

Novel approaches to inhibiting HIV-1 replication. Antiviral Res (2009) 1.48

Structural basis for high-affinity binding of LEDGF PWWP to mononucleosomes. Nucleic Acids Res (2013) 1.37

Structural biology of retroviral DNA integration. Virology (2011) 1.37

Structural basis for HIV-1 DNA integration in the human genome, role of the LEDGF/P75 cofactor. EMBO J (2009) 1.32

Natural polymorphisms of human immunodeficiency virus type 1 integrase and inherent susceptibilities to a panel of integrase inhibitors. Antimicrob Agents Chemother (2009) 1.27

Piecing together the structure of retroviral integrase, an important target in AIDS therapy. FEBS J (2009) 1.27

LEDGF/p75 proteins with alternative chromatin tethers are functional HIV-1 cofactors. PLoS Pathog (2009) 1.22

Protein delivery using engineered virus-like particles. Proc Natl Acad Sci U S A (2011) 1.22

Retroviral integrase proteins and HIV-1 DNA integration. J Biol Chem (2012) 1.20

HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor. Nucleic Acids Res (2012) 1.18

A new class of multimerization selective inhibitors of HIV-1 integrase. PLoS Pathog (2014) 1.16

Cryo-EM structures and atomic model of the HIV-1 strand transfer complex intasome. Science (2017) 1.15

An allosteric mechanism for inhibiting HIV-1 integrase with a small molecule. Mol Pharmacol (2009) 1.15

FRET analysis reveals distinct conformations of IN tetramers in the presence of viral DNA or LEDGF/p75. Nucleic Acids Res (2011) 1.15

The A128T resistance mutation reveals aberrant protein multimerization as the primary mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem (2013) 1.14

The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering. Nucleic Acids Res (2010) 1.08

Virological and cellular roles of the transcriptional coactivator LEDGF/p75. Curr Top Microbiol Immunol (2009) 1.07

Allosteric inhibition of human immunodeficiency virus integrase: late block during viral replication and abnormal multimerization involving specific protein domains. J Biol Chem (2014) 1.04

Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem (2009) 1.04

Dual inhibition of HIV-1 replication by integrase-LEDGF allosteric inhibitors is predominant at the post-integration stage. Retrovirology (2013) 1.04

TALEN knockout of the PSIP1 gene in human cells: analyses of HIV-1 replication and allosteric integrase inhibitor mechanism. J Virol (2014) 1.02

Affinities between the binding partners of the HIV-1 integrase dimer-lens epithelium-derived growth factor (IN dimer-LEDGF) complex. J Biol Chem (2009) 1.01

Mutations affecting interaction of integrase with TNPO3 do not prevent HIV-1 cDNA nuclear import. Retrovirology (2011) 1.01

Molecular mechanisms of retroviral integration site selection. Nucleic Acids Res (2014) 1.01

Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase. Retrovirology (2009) 0.99

Structural properties of HIV integrase. Lens epithelium-derived growth factor oligomers. J Biol Chem (2010) 0.98

Molecular dynamics approaches estimate the binding energy of HIV-1 integrase inhibitors and correlate with in vitro activity. Antimicrob Agents Chemother (2011) 0.97

Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding. Nucleic Acids Res (2014) 0.96

The HIV-1 integrase monomer induces a specific interaction with LTR DNA for concerted integration. Biochemistry (2011) 0.95

Implication of serine residues 271, 273, and 275 in the human immunodeficiency virus type 1 cofactor activity of lens epithelium-derived growth factor/p75. J Virol (2009) 0.93

Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus. J Virol (2012) 0.92

Retroviral integration: Site matters: Mechanisms and consequences of retroviral integration site selection. Bioessays (2015) 0.91

Small molecule inhibitors of the LEDGF site of human immunodeficiency virus integrase identified by fragment screening and structure based design. PLoS One (2012) 0.90

Retroviral DNA Integration. Chem Rev (2016) 0.90

The mechanism of H171T resistance reveals the importance of Nδ-protonated His171 for the binding of allosteric inhibitor BI-D to HIV-1 integrase. Retrovirology (2014) 0.90

Cryo-EM reveals a novel octameric integrase structure for betaretroviral intasome function. Nature (2016) 0.89

Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model. Mol Ther (2012) 0.88

LEDGF dominant interference proteins demonstrate prenuclear exposure of HIV-1 integrase and synergize with LEDGF depletion to destroy viral infectivity. J Virol (2011) 0.86

Interrogating HIV integrase for compounds that bind--a SAMPL challenge. J Comput Aided Mol Des (2014) 0.85

The Interaction Between Lentiviral Integrase and LEDGF: Structural and Functional Insights. Viruses (2009) 0.85

Non-Enzymatic Functions of Retroviral Integrase: The Next Target for Novel Anti-HIV Drug Development. Front Microbiol (2011) 0.85

Engineered hyperactive integrase for concerted HIV-1 DNA integration. PLoS One (2014) 0.85

HIV-1 Integrase-DNA Recognition Mechanisms. Viruses (2009) 0.84

Intasome architecture and chromatin density modulate retroviral integration into nucleosome. Retrovirology (2015) 0.83

Host Factors in Retroviral Integration and the Selection of Integration Target Sites. Microbiol Spectr (2014) 0.83

Transient Expression of an LEDGF/p75 Chimera Retargets Lentivector Integration and Functionally Rescues in a Model for X-CGD. Mol Ther Nucleic Acids (2013) 0.83

A critical role of the C-terminal segment for allosteric inhibitor-induced aberrant multimerization of HIV-1 integrase. J Biol Chem (2014) 0.83

Identification of low molecular weight nuclear complexes containing integrase during the early stages of HIV-1 infection. Retrovirology (2013) 0.83

Retroviral Integrase Structure and DNA Recombination Mechanism. Microbiol Spectr (2015) 0.83

A cooperative and specific DNA-binding mode of HIV-1 integrase depends on the nature of the metallic cofactor and involves the zinc-containing N-terminal domain. Nucleic Acids Res (2010) 0.83

Structural and functional role of INI1 and LEDGF in the HIV-1 preintegration complex. PLoS One (2013) 0.82

HIV-1 integrase multimerization as a therapeutic target. Curr Top Microbiol Immunol (2015) 0.81

Biochemical characterization of novel retroviral integrase proteins. PLoS One (2013) 0.81

The Competitive Interplay between Allosteric HIV-1 Integrase Inhibitor BI/D and LEDGF/p75 during the Early Stage of HIV-1 Replication Adversely Affects Inhibitor Potency. ACS Chem Biol (2016) 0.81

Sites of retroviral DNA integration: From basic research to clinical applications. Crit Rev Biochem Mol Biol (2015) 0.80

Integration site and clonal expansion in human chronic retroviral infection and gene therapy. Viruses (2014) 0.78

Efficient Transduction of LEDGF/p75 Mutant Cells by Gain-of-Function HIV-1 Integrase Mutant Viruses. Mol Ther Methods Clin Dev (2014) 0.78

Targeting HIV-1 DNA integration by swapping tethers. Proc Natl Acad Sci U S A (2010) 0.78

Prospective strategies for targeting HIV-1 integrase function. Future Med Chem (2010) 0.78

DNA Physical Properties and Nucleosome Positions Are Major Determinants of HIV-1 Integrase Selectivity. PLoS One (2015) 0.78

Alpharetroviral vectors: from a cancer-causing agent to a useful tool for human gene therapy. Viruses (2014) 0.77

C-Terminal Domain of Integrase Binds between the Two Active Sites. J Chem Theory Comput (2015) 0.76

Structural and functional insights into foamy viral integrase. Viruses (2013) 0.76

Comparison Between Several Integrase-defective Lentiviral Vectors Reveals Increased Integration of an HIV Vector Bearing a D167H Mutant. Mol Ther Nucleic Acids (2014) 0.76

The dynamics of interconverting D- and E-forms of the HIV-1 integrase N-terminal domain. Eur Biophys J (2014) 0.76

Multiple cellular proteins interact with LEDGF/p75 through a conserved unstructured consensus motif. Nat Commun (2015) 0.76

Structural Basis for Inhibitor-Induced Aggregation of HIV Integrase. PLoS Biol (2016) 0.75

Retroviral integrase protein and intasome nucleoprotein complex structures. World J Biol Chem (2017) 0.75

Drug resistant integrase mutants cause aberrant HIV integrations. Retrovirology (2016) 0.75

Anti-HIV drug development through computational methods. AAPS J (2014) 0.75

Impact of Chromatin on HIV Replication. Genes (Basel) (2015) 0.75

Mechanistic and pharmacological analyses of HIV-1 integration. Methods (2009) 0.75

The Preserved HTH-Docking Cleft of HIV-1 Integrase Is Functionally Critical. Structure (2016) 0.75

Articles cited by this

Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr (2004) 227.01

The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Crystallogr (1994) 187.88

Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr (1997) 137.43

Phaser crystallographic software. J Appl Crystallogr (2007) 108.34

PHENIX: building new software for automated crystallographic structure determination. Acta Crystallogr D Biol Crystallogr (2002) 53.61

LMO2-associated clonal T cell proliferation in two patients after gene therapy for SCID-X1. Science (2003) 19.77

Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases. Science (1994) 6.69

Ulp1-SUMO crystal structure and genetic analysis reveal conserved interactions and a regulatory element essential for cell growth in yeast. Mol Cell (2000) 6.39

Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. J Virol (1992) 6.07

HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells. J Biol Chem (2002) 4.91

A role for LEDGF/p75 in targeting HIV DNA integration. Nat Med (2005) 4.88

Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. EMBO J (1993) 4.39

Making and breaking nucleic acids: two-Mg2+-ion catalysis and substrate specificity. Mol Cell (2006) 4.26

Menin critically links MLL proteins with LEDGF on cancer-associated target genes. Cancer Cell (2008) 4.20

An essential role for LEDGF/p75 in HIV integration. Science (2006) 4.18

Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. Proc Natl Acad Sci U S A (1993) 3.99

LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells. J Biol Chem (2003) 3.97

LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev (2007) 3.96

The structural biology of HIV assembly. Curr Opin Struct Biol (2008) 3.71

Retroviral DNA integration: reaction pathway and critical intermediates. EMBO J (2006) 3.68

LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes. J Virol (2004) 3.45

Structure of a two-domain fragment of HIV-1 integrase: implications for domain organization in the intact protein. EMBO J (2001) 3.23

Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75. Proc Natl Acad Sci U S A (2005) 3.18

Three new structures of the core domain of HIV-1 integrase: an active site that binds magnesium. Proc Natl Acad Sci U S A (1998) 2.98

Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. Nat Struct Biol (1997) 2.81

Solution structure of the DNA binding domain of HIV-1 integrase. Biochemistry (1995) 2.69

Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase. J Biol Chem (2004) 2.64

The DNA-binding domain of HIV-1 integrase has an SH3-like fold. Nat Struct Biol (1995) 2.53

Structural implications of spectroscopic characterization of a putative zinc finger peptide from HIV-1 integrase. J Biol Chem (1992) 2.52

Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting. PLoS One (2007) 2.48

Integrase mutants defective for interaction with LEDGF/p75 are impaired in chromosome tethering and HIV-1 replication. J Biol Chem (2005) 2.44

The interaction of LEDGF/p75 with integrase is lentivirus-specific and promotes DNA binding. J Biol Chem (2005) 2.28

LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro. Nucleic Acids Res (2006) 2.22

Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering. J Cell Sci (2005) 2.19

Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75. Nat Struct Mol Biol (2005) 2.17

Identification and characterization of the chromatin-binding domains of the HIV-1 integrase interactor LEDGF/p75. J Mol Biol (2006) 2.13

Functional and structural characterization of the integrase from the prototype foamy virus. Nucleic Acids Res (2008) 2.07

A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. Nucleic Acids Res (2006) 2.02

The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication. PLoS Pathog (2008) 2.00

HIV-1 integrase crosslinked oligomers are active in vitro. Nucleic Acids Res (2005) 1.96

Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication. J Virol (2004) 1.94

The unfolding story of three-dimensional domain swapping. Structure (2003) 1.89

Processing of viral DNA ends channels the HIV-1 integration reaction to concerted integration. J Biol Chem (2005) 1.84

In vivo analysis of retroviral integrase structure and function. Adv Virus Res (1999) 1.82

Retroviral DNA integration--mechanism and consequences. Adv Genet (2005) 1.66

Recombinant human immunodeficiency virus type 1 integrase exhibits a capacity for full-site integration in vitro that is comparable to that of purified preintegration complexes from virus-infected cells. J Virol (2005) 1.60

The solution structure of the amino-terminal HHCC domain of HIV-2 integrase: a three-helix bundle stabilized by zinc. Curr Biol (1997) 1.44

LEDGF binds to heat shock and stress-related element to activate the expression of stress-related genes. Biochem Biophys Res Commun (2001) 1.44

Virus evolution reveals an exclusive role for LEDGF/p75 in chromosomal tethering of HIV. PLoS Pathog (2007) 1.41

Wild-type levels of human immunodeficiency virus type 1 infectivity in the absence of cellular emerin protein. J Virol (2006) 1.33

Functional oligomeric state of avian sarcoma virus integrase. J Biol Chem (2002) 1.31

Modulating target site selection during human immunodeficiency virus DNA integration in vitro with an engineered tethering factor. Hum Gene Ther (2006) 1.27

Activity of recombinant HIV-1 integrase on mini-HIV DNA. Nucleic Acids Res (1999) 1.22

Transcriptional coactivator LEDGF/p75 modulates human immunodeficiency virus type 1 integrase-mediated concerted integration. J Virol (2007) 1.21

Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness. Virology (2006) 1.17

Caspase cleavage of the nuclear autoantigen LEDGF/p75 abrogates its pro-survival function: implications for autoimmunity in atopic disorders. Cell Death Differ (2002) 1.15

LEDGF/p75 interferes with the formation of synaptic nucleoprotein complexes that catalyze full-site HIV-1 DNA integration in vitro: implications for the mechanism of viral cDNA integration. Virology (2007) 1.08

In vitro initial attachment of HIV-1 integrase to viral ends: control of the DNA specific interaction by the oligomerization state. Nucleic Acids Res (2008) 1.03

Articles by these authors

Identification of host proteins required for HIV infection through a functional genomic screen. Science (2008) 12.44

Retroviral intasome assembly and inhibition of DNA strand transfer. Nature (2010) 4.99

LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells. J Biol Chem (2003) 3.97

LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev (2007) 3.96

Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75. Proc Natl Acad Sci U S A (2005) 3.18

Role of Yersinia murine toxin in survival of Yersinia pestis in the midgut of the flea vector. Science (2002) 3.10

Flexible use of nuclear import pathways by HIV-1. Cell Host Microbe (2010) 3.08

Human immunodeficiency virus type 1 cDNAs produced in the presence of APOBEC3G exhibit defects in plus-strand DNA transfer and integration. J Virol (2007) 2.95

Improved molecular replacement by density- and energy-guided protein structure optimization. Nature (2011) 2.74

Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase. J Biol Chem (2004) 2.64

The mechanism of retroviral integration from X-ray structures of its key intermediates. Nature (2010) 2.58

The requirement for nucleoporin NUP153 during human immunodeficiency virus type 1 infection is determined by the viral capsid. J Virol (2011) 2.44

Proteasome inhibition reveals that a functional preintegration complex intermediate can be generated during restriction by diverse TRIM5 proteins. J Virol (2006) 2.32

Human cell proteins and human immunodeficiency virus DNA integration. Front Biosci (2004) 2.32

Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75. Nat Struct Mol Biol (2005) 2.17

Molecular mechanisms of retroviral integrase inhibition and the evolution of viral resistance. Proc Natl Acad Sci U S A (2010) 2.16

Structure-based modeling of the functional HIV-1 intasome and its inhibition. Proc Natl Acad Sci U S A (2010) 2.16

Functional and structural characterization of the integrase from the prototype foamy virus. Nucleic Acids Res (2008) 2.07

A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. Nucleic Acids Res (2006) 2.02

The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication. PLoS Pathog (2008) 2.00

The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase. J Virol (2009) 1.99

Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication. J Virol (2004) 1.94

Structural and functional analyses of the second-generation integrase strand transfer inhibitor dolutegravir (S/GSK1349572). Mol Pharmacol (2011) 1.87

Structural and functional analysis of a plant resistance protein TIR domain reveals interfaces for self-association, signaling, and autoregulation. Cell Host Microbe (2011) 1.83

Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1. Virology (2006) 1.80

The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes. J Virol (2003) 1.76

Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication. Virology (2005) 1.73

Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration. Proc Natl Acad Sci U S A (2010) 1.68

Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import. J Virol (2002) 1.68

Transcriptional co-activator p75 binds and tethers the Myc-interacting protein JPO2 to chromatin. J Cell Sci (2006) 1.67

Wild-type levels of nuclear localization and human immunodeficiency virus type 1 replication in the absence of the central DNA flap. J Virol (2002) 1.62

A molecular mechanism for bacterial susceptibility to zinc. PLoS Pathog (2011) 1.61

Dynamic modulation of HIV-1 integrase structure and function by cellular lens epithelium-derived growth factor (LEDGF) protein. J Biol Chem (2008) 1.61

Multimode, cooperative mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem (2012) 1.55

Evolution of a cytoplasmic tripartite motif (TRIM) protein in cows that restricts retroviral infection. Proc Natl Acad Sci U S A (2006) 1.53

Structural basis for functional tetramerization of lentiviral integrase. PLoS Pathog (2009) 1.53

The host proteins transportin SR2/TNPO3 and cyclophilin A exert opposing effects on HIV-1 uncoating. J Virol (2012) 1.52

The structural biology of HIV-1: mechanistic and therapeutic insights. Nat Rev Microbiol (2012) 1.50

3'-processing and strand transfer catalysed by retroviral integrase in crystallo. EMBO J (2012) 1.49

Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation. Proc Natl Acad Sci U S A (2013) 1.49

Centralspindlin links the mitotic spindle to the plasma membrane during cytokinesis. Nature (2012) 1.48

Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75. J Biol Chem (2004) 1.47

Structural biology of retroviral DNA integration. Virology (2011) 1.37

Nucleoporin NUP153 phenylalanine-glycine motifs engage a common binding pocket within the HIV-1 capsid protein to mediate lentiviral infectivity. PLoS Pathog (2013) 1.36

Human immunodeficiency virus type 1 capsid mutation N74D alters cyclophilin A dependence and impairs macrophage infection. J Virol (2012) 1.35

Bromo- and extraterminal domain chromatin regulators serve as cofactors for murine leukemia virus integration. J Virol (2013) 1.34

Wild-type levels of human immunodeficiency virus type 1 infectivity in the absence of cellular emerin protein. J Virol (2006) 1.33

Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase. J Virol (2005) 1.30

The SET complex acts as a barrier to autointegration of HIV-1. PLoS Pathog (2009) 1.28

Genetic analyses of conserved residues in the carboxyl-terminal domain of human immunodeficiency virus type 1 integrase. J Virol (2005) 1.28

Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. J Virol (2008) 1.27

Subunit-specific protein footprinting reveals significant structural rearrangements and a role for N-terminal Lys-14 of HIV-1 Integrase during viral DNA binding. J Biol Chem (2007) 1.26

Differential effects of human immunodeficiency virus type 1 capsid and cellular factors nucleoporin 153 and LEDGF/p75 on the efficiency and specificity of viral DNA integration. J Virol (2012) 1.24

Retroviral integrase proteins and HIV-1 DNA integration. J Biol Chem (2012) 1.20

HIV-1 exploits importin 7 to maximize nuclear import of its DNA genome. Retrovirology (2009) 1.20

HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor. Nucleic Acids Res (2012) 1.18

Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness. Virology (2006) 1.17

The A128T resistance mutation reveals aberrant protein multimerization as the primary mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem (2013) 1.14

New class of HIV integrase inhibitors that block viral replication in cell culture. Curr Biol (2002) 1.13

Intracellular transport of human immunodeficiency virus type 1 integrase. J Cell Sci (2003) 1.10

LEDGF/p75 interferes with the formation of synaptic nucleoprotein complexes that catalyze full-site HIV-1 DNA integration in vitro: implications for the mechanism of viral cDNA integration. Virology (2007) 1.08

Molecular mechanisms of HIV integration and therapeutic intervention. Expert Rev Mol Med (2007) 1.08

Solution conformations of prototype foamy virus integrase and its stable synaptic complex with U5 viral DNA. Structure (2012) 1.07

Chromatinized templates reveal the requirement for the LEDGF/p75 PWWP domain during HIV-1 integration in vitro. Nucleic Acids Res (2008) 1.07

pH6 antigen of Yersinia pestis interacts with plasma lipoproteins and cell membranes. J Lipid Res (2002) 1.05

Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem (2009) 1.04

env chimeric virus technology for evaluating human immunodeficiency virus susceptibility to entry inhibitors. Antimicrob Agents Chemother (2002) 1.04

Viral and cellular requirements for the nuclear entry of retroviral preintegration nucleoprotein complexes. Viruses (2013) 1.03

Lys-34, dispensable for integrase catalysis, is required for preintegration complex function and human immunodeficiency virus type 1 replication. J Virol (2005) 1.01

HIV DNA is heavily uracilated, which protects it from autointegration. Proc Natl Acad Sci U S A (2011) 1.00

VPS29 is not an active metallo-phosphatase but is a rigid scaffold required for retromer interaction with accessory proteins. PLoS One (2011) 0.99

Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase. Retrovirology (2009) 0.99

Structural basis for nuclear import of splicing factors by human Transportin 3. Proc Natl Acad Sci U S A (2014) 0.98

Activities, crystal structures, and molecular dynamics of dihydro-1H-isoindole derivatives, inhibitors of HIV-1 integrase. ACS Chem Biol (2012) 0.96

HIV-1 incorporates and proteolytically processes human NDR1 and NDR2 serine-threonine kinases. Virology (2005) 0.96

Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding. Nucleic Acids Res (2014) 0.96