Structural basis for the recognition between HIV-1 integrase and transcriptional coactivator p75.

PubWeight™: 3.18‹?› | Rank: Top 1%

🔗 View Article (PMC 1297672)

Published in Proc Natl Acad Sci U S A on October 31, 2005

Authors

Peter Cherepanov1, Andre L B Ambrosio, Shaila Rahman, Tom Ellenberger, Alan Engelman

Author Affiliations

1: Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA 02115, USA. alan_engelman@dfci.harvard.edu

Articles citing this

(truncated to the top 100)

LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev (2007) 3.96

Role of PSIP1/LEDGF/p75 in lentiviral infectivity and integration targeting. PLoS One (2007) 2.48

Rational design of small-molecule inhibitors of the LEDGF/p75-integrase interaction and HIV replication. Nat Chem Biol (2010) 2.48

LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro. Nucleic Acids Res (2006) 2.22

Functional and structural characterization of the integrase from the prototype foamy virus. Nucleic Acids Res (2008) 2.07

A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. Nucleic Acids Res (2006) 2.02

The lentiviral integrase binding protein LEDGF/p75 and HIV-1 replication. PLoS Pathog (2008) 2.00

Small-molecule inhibitors of protein-protein interactions: progressing toward the reality. Chem Biol (2014) 1.95

A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75. PLoS Pathog (2009) 1.89

Inhibiting HIV-1 integrase by shifting its oligomerization equilibrium. Proc Natl Acad Sci U S A (2007) 1.85

Overexpression of the lens epithelium-derived growth factor/p75 integrase binding domain inhibits human immunodeficiency virus replication. J Virol (2006) 1.82

Psip1/Ledgf p52 binds methylated histone H3K36 and splicing factors and contributes to the regulation of alternative splicing. PLoS Genet (2012) 1.82

Natural variation of HIV-1 group M integrase: implications for a new class of antiretroviral inhibitors. Retrovirology (2008) 1.79

Resistance to integrase inhibitors. Viruses (2010) 1.75

HIV DNA integration. Cold Spring Harb Perspect Med (2012) 1.73

Dynamic modulation of HIV-1 integrase structure and function by cellular lens epithelium-derived growth factor (LEDGF) protein. J Biol Chem (2008) 1.61

Multimode, cooperative mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem (2012) 1.55

In-Silico docking of HIV-1 integrase inhibitors reveals a novel drug type acting on an enzyme/DNA reaction intermediate. Retrovirology (2007) 1.55

Structural basis for functional tetramerization of lentiviral integrase. PLoS Pathog (2009) 1.53

New class of HIV-1 integrase (IN) inhibitors with a dual mode of action. J Biol Chem (2012) 1.52

Disruption of Ledgf/Psip1 results in perinatal mortality and homeotic skeletal transformations. Mol Cell Biol (2006) 1.51

Integrase, LEDGF/p75 and HIV replication. Cell Mol Life Sci (2008) 1.50

The structural biology of HIV-1: mechanistic and therapeutic insights. Nat Rev Microbiol (2012) 1.50

Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation. Proc Natl Acad Sci U S A (2013) 1.49

Integrase and integration: biochemical activities of HIV-1 integrase. Retrovirology (2008) 1.48

BET proteins promote efficient murine leukemia virus integration at transcription start sites. Proc Natl Acad Sci U S A (2013) 1.47

Virus evolution reveals an exclusive role for LEDGF/p75 in chromosomal tethering of HIV. PLoS Pathog (2007) 1.41

Small-molecule inhibitors of the LEDGF/p75 binding site of integrase block HIV replication and modulate integrase multimerization. Antimicrob Agents Chemother (2012) 1.40

Structural basis for high-affinity binding of LEDGF PWWP to mononucleosomes. Nucleic Acids Res (2013) 1.37

Structural biology of retroviral DNA integration. Virology (2011) 1.37

Structural basis for HIV-1 DNA integration in the human genome, role of the LEDGF/P75 cofactor. EMBO J (2009) 1.32

Lens epithelium-derived growth factor/p75 interacts with the transposase-derived DDE domain of PogZ. J Biol Chem (2009) 1.30

Natural polymorphisms of human immunodeficiency virus type 1 integrase and inherent susceptibilities to a panel of integrase inhibitors. Antimicrob Agents Chemother (2009) 1.27

Piecing together the structure of retroviral integrase, an important target in AIDS therapy. FEBS J (2009) 1.27

Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. J Virol (2008) 1.27

LEDGF/p75-independent HIV-1 replication demonstrates a role for HRP-2 and remains sensitive to inhibition by LEDGINs. PLoS Pathog (2012) 1.25

Gammaretroviral integration into nucleosomal target DNA in vivo. J Virol (2011) 1.23

LEDGF/p75 proteins with alternative chromatin tethers are functional HIV-1 cofactors. PLoS Pathog (2009) 1.22

Transcriptional coactivator LEDGF/p75 modulates human immunodeficiency virus type 1 integrase-mediated concerted integration. J Virol (2007) 1.21

Retroviral integrase proteins and HIV-1 DNA integration. J Biol Chem (2012) 1.20

Discovery of BI 224436, a Noncatalytic Site Integrase Inhibitor (NCINI) of HIV-1. ACS Med Chem Lett (2014) 1.20

HIV-1 exploits importin 7 to maximize nuclear import of its DNA genome. Retrovirology (2009) 1.20

Features of protein-protein interactions that translate into potent inhibitors: topology, surface area and affinity. Expert Rev Mol Med (2012) 1.18

HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor. Nucleic Acids Res (2012) 1.18

Discovery of a small-molecule HIV-1 integrase inhibitor-binding site. Proc Natl Acad Sci U S A (2006) 1.16

A new class of multimerization selective inhibitors of HIV-1 integrase. PLoS Pathog (2014) 1.16

An allosteric mechanism for inhibiting HIV-1 integrase with a small molecule. Mol Pharmacol (2009) 1.15

FRET analysis reveals distinct conformations of IN tetramers in the presence of viral DNA or LEDGF/p75. Nucleic Acids Res (2011) 1.15

The A128T resistance mutation reveals aberrant protein multimerization as the primary mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem (2013) 1.14

Enhancers are major targets for murine leukemia virus vector integration. J Virol (2014) 1.10

Retroviral integration site selection. Viruses (2010) 1.10

The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering. Nucleic Acids Res (2010) 1.08

von Hippel Lindau binding protein 1-mediated degradation of integrase affects HIV-1 gene expression at a postintegration step. Proc Natl Acad Sci U S A (2007) 1.08

Virological and cellular roles of the transcriptional coactivator LEDGF/p75. Curr Top Microbiol Immunol (2009) 1.07

Chromatinized templates reveal the requirement for the LEDGF/p75 PWWP domain during HIV-1 integration in vitro. Nucleic Acids Res (2008) 1.07

Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem (2009) 1.04

Dual inhibition of HIV-1 replication by integrase-LEDGF allosteric inhibitors is predominant at the post-integration stage. Retrovirology (2013) 1.04

Revealing domain structure through linker-scanning analysis of the murine leukemia virus (MuLV) RNase H and MuLV and human immunodeficiency virus type 1 integrase proteins. J Virol (2006) 1.03

Role of the PWWP domain of lens epithelium-derived growth factor (LEDGF)/p75 cofactor in lentiviral integration targeting. J Biol Chem (2011) 1.03

TALEN knockout of the PSIP1 gene in human cells: analyses of HIV-1 replication and allosteric integrase inhibitor mechanism. J Virol (2014) 1.02

Affinities between the binding partners of the HIV-1 integrase dimer-lens epithelium-derived growth factor (IN dimer-LEDGF) complex. J Biol Chem (2009) 1.01

Mutations affecting interaction of integrase with TNPO3 do not prevent HIV-1 cDNA nuclear import. Retrovirology (2011) 1.01

Defining the DNA substrate binding sites on HIV-1 integrase. J Mol Biol (2008) 1.01

Molecular mechanisms of retroviral integration site selection. Nucleic Acids Res (2014) 1.01

The role of lysine 186 in HIV-1 integrase multimerization. Virology (2007) 1.00

Allosteric inhibitor development targeting HIV-1 integrase. ChemMedChem (2011) 1.00

Blind prediction of HIV integrase binding from the SAMPL4 challenge. J Comput Aided Mol Des (2014) 0.99

Allosteric inhibition of HIV-1 integrase activity. Curr Opin Chem Biol (2013) 0.99

Structural properties of HIV integrase. Lens epithelium-derived growth factor oligomers. J Biol Chem (2010) 0.98

Molecular dynamics approaches estimate the binding energy of HIV-1 integrase inhibitors and correlate with in vitro activity. Antimicrob Agents Chemother (2011) 0.97

The molecular biology of HIV integrase. Future Virol (2012) 0.97

Inhibiting the HIV integration process: past, present, and the future. J Med Chem (2013) 0.93

Implication of serine residues 271, 273, and 275 in the human immunodeficiency virus type 1 cofactor activity of lens epithelium-derived growth factor/p75. J Virol (2009) 0.93

Multimodal mechanism of action of allosteric HIV-1 integrase inhibitors. Expert Rev Mol Med (2013) 0.92

Correlation of recombinant integrase activity and functional preintegration complex formation during acute infection by replication-defective integrase mutant human immunodeficiency virus. J Virol (2012) 0.92

BET-independent MLV-based Vectors Target Away From Promoters and Regulatory Elements. Mol Ther Nucleic Acids (2014) 0.91

Determinants that specify the integration pattern of retrotransposon Tf1 in the fbp1 promoter of Schizosaccharomyces pombe. J Virol (2010) 0.91

Retroviral integration: Site matters: Mechanisms and consequences of retroviral integration site selection. Bioessays (2015) 0.91

Small molecule inhibitors of the LEDGF site of human immunodeficiency virus integrase identified by fragment screening and structure based design. PLoS One (2012) 0.90

Retroviral DNA Integration. Chem Rev (2016) 0.90

The mechanism of H171T resistance reveals the importance of Nδ-protonated His171 for the binding of allosteric inhibitor BI-D to HIV-1 integrase. Retrovirology (2014) 0.90

Dolutegravir interactions with HIV-1 integrase-DNA: structural rationale for drug resistance and dissociation kinetics. PLoS One (2013) 0.89

Phage display-directed discovery of LEDGF/p75 binding cyclic peptide inhibitors of HIV replication. Mol Ther (2012) 0.89

LEDGF/p75 interacts with mRNA splicing factors and targets HIV-1 integration to highly spliced genes. Genes Dev (2015) 0.89

Lens epithelium-derived growth factor/p75 qualifies as a target for HIV gene therapy in the NSG mouse model. Mol Ther (2012) 0.88

Uneven genetic robustness of HIV-1 integrase. J Virol (2014) 0.88

3-Hydroxypyrimidine-2,4-diones as an inhibitor scaffold of HIV integrase. J Med Chem (2011) 0.87

SUMOylation of the lens epithelium-derived growth factor/p75 attenuates its transcriptional activity on the heat shock protein 27 promoter. J Mol Biol (2010) 0.87

Characterization of the HIV-1 integrase chromatin- and LEDGF/p75-binding abilities by mutagenic analysis within the catalytic core domain of integrase. Virol J (2010) 0.86

Measuring rapid hydrogen exchange in the homodimeric 36 kDa HIV-1 integrase catalytic core domain. Protein Sci (2011) 0.86

LEDGF dominant interference proteins demonstrate prenuclear exposure of HIV-1 integrase and synergize with LEDGF depletion to destroy viral infectivity. J Virol (2011) 0.86

Design of HIV-1 integrase inhibitors targeting the catalytic domain as well as its interaction with LEDGF/p75: a scaffold hopping approach using salicylate and catechol groups. Bioorg Med Chem (2011) 0.85

Interrogating HIV integrase for compounds that bind--a SAMPL challenge. J Comput Aided Mol Des (2014) 0.85

The Interaction Between Lentiviral Integrase and LEDGF: Structural and Functional Insights. Viruses (2009) 0.85

Mass spectrometry-based footprinting of protein-protein interactions. Methods (2008) 0.84

Allosteric modulation of protein oligomerization: an emerging approach to drug design. Front Chem (2014) 0.84

Bimodal high-affinity association of Brd4 with murine leukemia virus integrase and mononucleosomes. Nucleic Acids Res (2014) 0.84

TALEN gene editing takes aim on HIV. Hum Genet (2016) 0.84

Inhibition of HIV-1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signal. Retrovirology (2009) 0.83

A critical role of the C-terminal segment for allosteric inhibitor-induced aberrant multimerization of HIV-1 integrase. J Biol Chem (2014) 0.83

Articles cited by this

Coot: model-building tools for molecular graphics. Acta Crystallogr D Biol Crystallogr (2004) 227.01

The CCP4 suite: programs for protein crystallography. Acta Crystallogr D Biol Crystallogr (1994) 187.88

Crystallography & NMR system: A new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr (1998) 169.28

Refinement of macromolecular structures by the maximum-likelihood method. Acta Crystallogr D Biol Crystallogr (1997) 137.43

Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallogr A (1991) 119.63

MOLMOL: a program for display and analysis of macromolecular structures. J Mol Graph (1996) 39.73

In vivo activation of the p53 pathway by small-molecule antagonists of MDM2. Science (2004) 24.42

ESPript: analysis of multiple sequence alignments in PostScript. Bioinformatics (1999) 21.85

The atomic structure of protein-protein recognition sites. J Mol Biol (1999) 12.63

Macromolecular TLS refinement in REFMAC at moderate resolutions. Methods Enzymol (2003) 9.69

Crystal structure of the catalytic domain of HIV-1 integrase: similarity to other polynucleotidyl transferases. Science (1994) 6.69

Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. J Virol (1992) 6.07

Small-molecule antagonists of the oncogenic Tcf/beta-catenin protein complex. Cancer Cell (2004) 4.98

HIV-1 integrase forms stable tetramers and associates with LEDGF/p75 protein in human cells. J Biol Chem (2002) 4.91

Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. EMBO J (1993) 4.39

Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. Proc Natl Acad Sci U S A (1993) 3.99

LEDGF/p75 is essential for nuclear and chromosomal targeting of HIV-1 integrase in human cells. J Biol Chem (2003) 3.97

Comparison of ARM and HEAT protein repeats. J Mol Biol (2001) 3.78

LEDGF/p75 determines cellular trafficking of diverse lentiviral but not murine oncoretroviral integrase proteins and is a component of functional lentiviral preintegration complexes. J Virol (2004) 3.45

Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type I integrase protein. Nucleic Acids Res (1993) 3.42

Structure of a two-domain fragment of HIV-1 integrase: implications for domain organization in the intact protein. EMBO J (2001) 3.23

Three new structures of the core domain of HIV-1 integrase: an active site that binds magnesium. Proc Natl Acad Sci U S A (1998) 2.98

Targeting survival: integration site selection by retroviruses and LTR-retrotransposons. Cell (2003) 2.84

Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. Nat Struct Biol (1997) 2.81

Solution structure of the DNA binding domain of HIV-1 integrase. Biochemistry (1995) 2.69

Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase. J Biol Chem (2004) 2.64

A soluble active mutant of HIV-1 integrase: involvement of both the core and carboxyl-terminal domains in multimerization. J Biol Chem (1996) 2.58

The DNA-binding domain of HIV-1 integrase has an SH3-like fold. Nat Struct Biol (1995) 2.53

Crystal structures of the catalytic domain of HIV-1 integrase free and complexed with its metal cofactor: high level of similarity of the active site with other viral integrases. J Mol Biol (1998) 2.49

Zinc folds the N-terminal domain of HIV-1 integrase, promotes multimerization, and enhances catalytic activity. Proc Natl Acad Sci U S A (1996) 2.46

Integrase mutants defective for interaction with LEDGF/p75 are impaired in chromosome tethering and HIV-1 replication. J Biol Chem (2005) 2.44

Human cell proteins and human immunodeficiency virus DNA integration. Front Biosci (2004) 2.32

The interaction of LEDGF/p75 with integrase is lentivirus-specific and promotes DNA binding. J Biol Chem (2005) 2.28

HIV-1 infection requires a functional integrase NLS. Mol Cell (2001) 2.26

Identification of the LEDGF/p75 HIV-1 integrase-interaction domain and NLS reveals NLS-independent chromatin tethering. J Cell Sci (2005) 2.19

Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75. Nat Struct Mol Biol (2005) 2.17

Catalytic domain of human immunodeficiency virus type 1 integrase: identification of a soluble mutant by systematic replacement of hydrophobic residues. Proc Natl Acad Sci U S A (1995) 2.09

Comparative architecture of transposase and integrase complexes. Nat Struct Biol (2001) 1.99

Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication. J Virol (2004) 1.94

Lens epithelium-derived growth factor/p75 prevents proteasomal degradation of HIV-1 integrase. J Biol Chem (2004) 1.79

Crystal structure of an active two-domain derivative of Rous sarcoma virus integrase. J Mol Biol (2000) 1.69

Zn2+ promotes the self-association of human immunodeficiency virus type-1 integrase in vitro. Biochemistry (1997) 1.69

X-ray structure of simian immunodeficiency virus integrase containing the core and C-terminal domain (residues 50-293)--an initial glance of the viral DNA binding platform. J Mol Biol (2000) 1.63

Oligomerization within virions and subcellular localization of human immunodeficiency virus type 1 integrase. J Virol (1999) 1.52

Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75. J Biol Chem (2004) 1.47

Identification of a small-molecule binding site at the dimer interface of the HIV integrase catalytic domain. Acta Crystallogr D Biol Crystallogr (2001) 1.39

Nuclear localization of human immunodeficiency virus type 1 integrase expressed as a fusion protein with green fluorescent protein. Virology (1999) 1.27

Biophysical and enzymatic properties of the catalytic domain of HIV-1 integrase. J Biol Chem (1994) 1.22

RNA recognition via the SAM domain of Smaug. Mol Cell (2003) 1.22

The ups and downs of gene expression and retroviral DNA integration. Proc Natl Acad Sci U S A (2005) 1.17

High-level expression of active HIV-1 integrase from a synthetic gene in human cells. FASEB J (2000) 1.13

Identification of an inhibitor-binding site to HIV-1 integrase with affinity acetylation and mass spectrometry. Proc Natl Acad Sci U S A (2004) 1.13

Atomic resolution structures of the core domain of avian sarcoma virus integrase and its D64N mutant. Biochemistry (1999) 1.04

Chicoric acid analogues as HIV-1 integrase inhibitors. J Med Chem (1999) 0.95

Articles by these authors

Identification of host proteins required for HIV infection through a functional genomic screen. Science (2008) 12.44

Targeting mitochondrial glutaminase activity inhibits oncogenic transformation. Cancer Cell (2010) 5.02

Retroviral intasome assembly and inhibition of DNA strand transfer. Nature (2010) 4.99

LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration. Genes Dev (2007) 3.96

Single-molecule kinetics of lambda exonuclease reveal base dependence and dynamic disorder. Science (2003) 3.22

Flexible use of nuclear import pathways by HIV-1. Cell Host Microbe (2010) 3.08

Human immunodeficiency virus type 1 cDNAs produced in the presence of APOBEC3G exhibit defects in plus-strand DNA transfer and integration. J Virol (2007) 2.95

Human DNA ligase I completely encircles and partially unwinds nicked DNA. Nature (2004) 2.85

Identification of an evolutionarily conserved domain in human lens epithelium-derived growth factor/transcriptional co-activator p75 (LEDGF/p75) that binds HIV-1 integrase. J Biol Chem (2004) 2.64

DNA ligases: structure, reaction mechanism, and function. Chem Rev (2006) 2.57

The requirement for nucleoporin NUP153 during human immunodeficiency virus type 1 infection is determined by the viral capsid. J Virol (2011) 2.44

Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase. EMBO J (2004) 2.43

Proteasome inhibition reveals that a functional preintegration complex intermediate can be generated during restriction by diverse TRIM5 proteins. J Virol (2006) 2.32

Human cell proteins and human immunodeficiency virus DNA integration. Front Biosci (2004) 2.32

A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA. Mol Cell (2006) 2.23

Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75. Nat Struct Mol Biol (2005) 2.17

Structure-based modeling of the functional HIV-1 intasome and its inhibition. Proc Natl Acad Sci U S A (2010) 2.16

A structural basis for allosteric control of DNA recombination by lambda integrase. Nature (2005) 2.05

A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo. Nucleic Acids Res (2006) 2.02

The requirement for cellular transportin 3 (TNPO3 or TRN-SR2) during infection maps to human immunodeficiency virus type 1 capsid and not integrase. J Virol (2009) 1.99

The crystal structure of the bifunctional primase-helicase of bacteriophage T7. Mol Cell (2003) 1.99

Class II integrase mutants with changes in putative nuclear localization signals are primarily blocked at a postnuclear entry step of human immunodeficiency virus type 1 replication. J Virol (2004) 1.94

A novel co-crystal structure affords the design of gain-of-function lentiviral integrase mutants in the presence of modified PSIP1/LEDGF/p75. PLoS Pathog (2009) 1.89

Dissecting the broad substrate specificity of human 3-methyladenine-DNA glycosylase. J Biol Chem (2003) 1.82

Requirements for capsid-binding and an effector function in TRIMCyp-mediated restriction of HIV-1. Virology (2006) 1.80

Chk1-mediated phosphorylation of FANCE is required for the Fanconi anemia/BRCA pathway. Mol Cell Biol (2007) 1.77

A lysine residue in the fingers subdomain of T7 DNA polymerase modulates the miscoding potential of 8-oxo-7,8-dihydroguanosine. Structure (2005) 1.76

The barrier-to-autointegration factor is a component of functional human immunodeficiency virus type 1 preintegration complexes. J Virol (2003) 1.76

Biochemical and genetic analyses of integrase-interacting proteins lens epithelium-derived growth factor (LEDGF)/p75 and hepatoma-derived growth factor related protein 2 (HRP2) in preintegration complex function and HIV-1 replication. Virology (2005) 1.73

Srs2 disassembles Rad51 filaments by a protein-protein interaction triggering ATP turnover and dissociation of Rad51 from DNA. Mol Cell (2009) 1.73

Structure of the coiled-coil dimerization motif of Sir4 and its interaction with Sir3. Structure (2003) 1.71

Lens epithelium-derived growth factor fusion proteins redirect HIV-1 DNA integration. Proc Natl Acad Sci U S A (2010) 1.68

Nuclear localization of human immunodeficiency virus type 1 preintegration complexes (PICs): V165A and R166A are pleiotropic integrase mutants primarily defective for integration, not PIC nuclear import. J Virol (2002) 1.68

Transcriptional co-activator p75 binds and tethers the Myc-interacting protein JPO2 to chromatin. J Cell Sci (2006) 1.67

Domain-swapped dimerization of the HIV-1 capsid C-terminal domain. Proc Natl Acad Sci U S A (2007) 1.66

Wild-type levels of nuclear localization and human immunodeficiency virus type 1 replication in the absence of the central DNA flap. J Virol (2002) 1.62

Modular architecture of the bacteriophage T7 primase couples RNA primer synthesis to DNA synthesis. Mol Cell (2003) 1.61

Dynamic modulation of HIV-1 integrase structure and function by cellular lens epithelium-derived growth factor (LEDGF) protein. J Biol Chem (2008) 1.61

Multimode, cooperative mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem (2012) 1.55

Crystal structure and DNA binding functions of ERCC1, a subunit of the DNA structure-specific endonuclease XPF-ERCC1. Proc Natl Acad Sci U S A (2005) 1.54

Structural basis for functional tetramerization of lentiviral integrase. PLoS Pathog (2009) 1.53

Evolution of a cytoplasmic tripartite motif (TRIM) protein in cows that restricts retroviral infection. Proc Natl Acad Sci U S A (2006) 1.53

The host proteins transportin SR2/TNPO3 and cyclophilin A exert opposing effects on HIV-1 uncoating. J Virol (2012) 1.52

Mitochondrial localization and structure-based phosphate activation mechanism of Glutaminase C with implications for cancer metabolism. Proc Natl Acad Sci U S A (2012) 1.51

Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation. Proc Natl Acad Sci U S A (2013) 1.49

Identification and characterization of a functional nuclear localization signal in the HIV-1 integrase interactor LEDGF/p75. J Biol Chem (2004) 1.47

A nonhistone protein-protein interaction required for assembly of the SIR complex and silent chromatin. Mol Cell Biol (2005) 1.42

Human alkyladenine DNA glycosylase uses acid-base catalysis for selective excision of damaged purines. Biochemistry (2003) 1.42

Structure-based drug design and optimization of mannoside bacterial FimH antagonists. J Med Chem (2010) 1.40

Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7. Nat Struct Mol Biol (2004) 1.40

The Escherichia coli 3-methyladenine DNA glycosylase AlkA has a remarkably versatile active site. J Biol Chem (2004) 1.39

The molecular basis for the regulation of the cap-binding complex by the importins. Nat Struct Mol Biol (2009) 1.38

Structural biology of retroviral DNA integration. Virology (2011) 1.37

Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair. Cancer Res (2008) 1.36

Nucleoporin NUP153 phenylalanine-glycine motifs engage a common binding pocket within the HIV-1 capsid protein to mediate lentiviral infectivity. PLoS Pathog (2013) 1.36

Human immunodeficiency virus type 1 capsid mutation N74D alters cyclophilin A dependence and impairs macrophage infection. J Virol (2012) 1.35

Identification of a peptide fragment of DSCR1 that competitively inhibits calcineurin activity in vitro and in vivo. Proc Natl Acad Sci U S A (2005) 1.33

Wild-type levels of human immunodeficiency virus type 1 infectivity in the absence of cellular emerin protein. J Virol (2006) 1.33

Human DNA ligase III recognizes DNA ends by dynamic switching between two DNA-bound states. Biochemistry (2010) 1.32

Structural dynamics in DNA damage signaling and repair. Curr Opin Struct Biol (2010) 1.31

Genetic analyses of DNA-binding mutants in the catalytic core domain of human immunodeficiency virus type 1 integrase. J Virol (2005) 1.30

Two DNA-binding and nick recognition modules in human DNA ligase III. J Biol Chem (2008) 1.30

Functional evidence for a small and rigid active site in a high fidelity DNA polymerase: probing T7 DNA polymerase with variably sized base pairs. J Biol Chem (2005) 1.29

Multiple interactions with the Rad51 recombinase govern the homologous recombination function of Rad54. J Biol Chem (2004) 1.28

The SET complex acts as a barrier to autointegration of HIV-1. PLoS Pathog (2009) 1.28

Genetic analyses of conserved residues in the carboxyl-terminal domain of human immunodeficiency virus type 1 integrase. J Virol (2005) 1.28

Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity. J Virol (2008) 1.27

Subunit-specific protein footprinting reveals significant structural rearrangements and a role for N-terminal Lys-14 of HIV-1 Integrase during viral DNA binding. J Biol Chem (2007) 1.26

Structural determinants for inhibitor specificity and selectivity in PDE2A using the wheat germ in vitro translation system. Biochemistry (2005) 1.24

Differential effects of human immunodeficiency virus type 1 capsid and cellular factors nucleoporin 153 and LEDGF/p75 on the efficiency and specificity of viral DNA integration. J Virol (2012) 1.24

Structural basis for the recruitment of ERCC1-XPF to nucleotide excision repair complexes by XPA. EMBO J (2007) 1.23

Structural rearrangements in the thyroid hormone receptor hinge domain and their putative role in the receptor function. J Mol Biol (2006) 1.22

Crystal structures of 2-acetylaminofluorene and 2-aminofluorene in complex with T7 DNA polymerase reveal mechanisms of mutagenesis. Proc Natl Acad Sci U S A (2004) 1.21

Retroviral integrase proteins and HIV-1 DNA integration. J Biol Chem (2012) 1.20

A conformational switch controls the DNA cleavage activity of lambda integrase. Mol Cell (2003) 1.19

The XPA-binding domain of ERCC1 is required for nucleotide excision repair but not other DNA repair pathways. J Biol Chem (2009) 1.18

HRP2 determines the efficiency and specificity of HIV-1 integration in LEDGF/p75 knockout cells but does not contribute to the antiviral activity of a potent LEDGF/p75-binding site integrase inhibitor. Nucleic Acids Res (2012) 1.18

Structure-based mutagenesis of the integrase-LEDGF/p75 interface uncouples a strict correlation between in vitro protein binding and HIV-1 fitness. Virology (2006) 1.17

Structure of mammalian poly(ADP-ribose) glycohydrolase reveals a flexible tyrosine clasp as a substrate-binding element. Nat Struct Mol Biol (2012) 1.16

The A128T resistance mutation reveals aberrant protein multimerization as the primary mechanism of action of allosteric HIV-1 integrase inhibitors. J Biol Chem (2013) 1.14

Identification and validation of human DNA ligase inhibitors using computer-aided drug design. J Med Chem (2008) 1.10

Intracellular transport of human immunodeficiency virus type 1 integrase. J Cell Sci (2003) 1.10

A molecular handoff between bacteriophage T7 DNA primase and T7 DNA polymerase initiates DNA synthesis. J Biol Chem (2004) 1.09

LEDGF/p75 interferes with the formation of synaptic nucleoprotein complexes that catalyze full-site HIV-1 DNA integration in vitro: implications for the mechanism of viral cDNA integration. Virology (2007) 1.08

Molecular mechanisms of HIV integration and therapeutic intervention. Expert Rev Mol Med (2007) 1.08

Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases. EMBO J (2003) 1.07

Domain structure and protein interactions of the silent information regulator Sir3 revealed by screening a nested deletion library of protein fragments. J Biol Chem (2006) 1.07

Chromatinized templates reveal the requirement for the LEDGF/p75 PWWP domain during HIV-1 integration in vitro. Nucleic Acids Res (2008) 1.07

Arm sequences contribute to the architecture and catalytic function of a lambda integrase-Holliday junction complex. Mol Cell (2003) 1.06

Transcriptional co-activator LEDGF interacts with Cdc7-activator of S-phase kinase (ASK) and stimulates its enzymatic activity. J Biol Chem (2009) 1.04

Human DNA ligases I, III, and IV-purification and new specific assays for these enzymes. Methods Enzymol (2006) 1.04

Viral and cellular requirements for the nuclear entry of retroviral preintegration nucleoprotein complexes. Viruses (2013) 1.03

Differential affinity and cooperativity functions of the amino-terminal 70 residues of lambda integrase. J Mol Biol (2002) 1.02

DNA arms do the legwork to ensure the directionality of lambda site-specific recombination. Curr Opin Struct Biol (2005) 1.02

Lys-34, dispensable for integrase catalysis, is required for preintegration complex function and human immunodeficiency virus type 1 replication. J Virol (2005) 1.01

HIV DNA is heavily uracilated, which protects it from autointegration. Proc Natl Acad Sci U S A (2011) 1.00

Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase. Retrovirology (2009) 0.99